1g9d

CRYSTAL STRUCTURE OF CLOSTRIDIUM BOTULINUM NEUROTOXIN B COMPLEXED WITH AN INHIBITOR (EXPERIMENT 2)

Method: X-RAY DIFFRACTION Dmax: 142.3 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1g9d

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1g9d
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1g9d
沉积日期 deposition_date2000-11-22
结构标题 titleCRYSTAL STRUCTURE OF CLOSTRIDIUM BOTULINUM NEUROTOXIN B COMPLEXED WITH AN INHIBITOR (EXPERIMENT 2)
关键词 keywordsbotulinum, neurotoxin, inhibitor, complex, HYDROLASE; HYDROLASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier40.56
回转半径 Rg (电子) rg_electron40.47
零角强度 I(0) i0316774000.00
分子量 molecular_weight150600.0 kDa
排除体积 excluded_volume190360 ų
包络体积 envelope_volume244330 ų
水化壳体积 shell_volume51609 ų
包络直径 envelope_diameter153.1
壳层 Rg shell_rg44.38
包络 Rg envelope_rg40.57
形状 Rg shape_rg40.49
总 Rg total_rg40.64
总原子数 total_atoms10638
残基数 n_residues1287
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax142.3
Rg (实空间) rg_real40.72
Rg 误差 (实空间) rg_real_error1.52
I(0) (实空间) i0_real3.1680e+08
I(0) 误差 (实空间) i0_real_error5.9180e+06
Rg (倒空间) rg_reciprocal40.56
I(0) (倒空间) i0_reciprocal316700000.0000
解质量估计 total_estimate0.8540
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary40.1
偏度 Skewness skewness0.454
峰度 Kurtosis kurtosis-0.291
角度范围 angular_range— – 0.1950 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha51420000.0000
实空间数据点数 n_real_points40
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.800; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.836; Smooth: 0.862

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (4)

6. 折叠分类 (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

结构域编号 domain_idd1g9da1
类 Class classb — All beta proteins
折叠类型 Fold foldb.29 — Concanavalin A-like lectins/glucanases
超家族 Superfamily superfamilyb.29.1 — Concanavalin A-like lectins/glucanases
家族 Family familyb.29.1.6 — Clostridium neurotoxins, the second last domain
结构域编号 domain_idd1g9da2
类 Class classb — All beta proteins
折叠类型 Fold foldb.42 — beta-Trefoil
超家族 Superfamily superfamilyb.42.4 — STI-like
家族 Family familyb.42.4.2 — Clostridium neurotoxins, C-terminal domain
结构域编号 domain_idd1g9da3
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.92 — Zincin-like
超家族 Superfamily superfamilyd.92.1 — Metalloproteases ('zincins'), catalytic domain
家族 Family familyd.92.1.7 — Clostridium neurotoxins, catalytic domain
结构域编号 domain_idd1g9da4
类 Class classh — Coiled coil proteins
折叠类型 Fold foldh.4 — Antiparallel coiled-coil
超家族 Superfamily superfamilyh.4.2 — Clostridium neurotoxins, 'coiled-coil' domain
家族 Family familyh.4.2.1 — Clostridium neurotoxins, 'coiled-coil' domain

CATH v4.4 (4 domains)

结构域编号 domain_id1g9dA01
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1240 — Zincin-like
同源超家族 H-superfamily homologous superfamily10 — Metalloproteases ("zincins"), catalytic domain like
结构域编号 domain_id1g9dA02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1120 — Clostridium botulinum neurotoxin B, "coiled-coil" domain
同源超家族 H-superfamily homologous superfamily10 — Clostridium botulinum neurotoxin b, "coiled-coil" domain
结构域编号 domain_id1g9dA03
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology120 — Jelly Rolls
同源超家族 H-superfamily homologous superfamily200
结构域编号 domain_id1g9dA04
类 Class class2 — Mainly Beta
架构 Architecture architecture80 — Trefoil
拓扑 Topology topology10 — Trefoil (Acidic Fibroblast Growth Factor, subunit A)
同源超家族 H-superfamily homologous superfamily50

7. 引用文献 (3)

8. 文件与曲线 (10)