1hc1

CRYSTAL STRUCTURE OF HEXAMERIC HAEMOCYANIN FROM PANULIRUS INTERRUPTUS REFINED AT 3.2 ANGSTROMS RESOLUTION

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

ARTHROPODAN HEMOCYANIN

Panulirus interruptus

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 6	COPPER (II) ION × 12 water × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	HCYA_PANIN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–657； UniProt 1–657 Author chain B； PDBConstruct 1–657； UniProt 1–657 Author chain C； PDBConstruct 1–657； UniProt 1–657 Author chain D； PDBConstruct 1–657； UniProt 1–657 Author chain E； PDBConstruct 1–657； UniProt 1–657 Author chain F； PDBConstruct 1–657； UniProt 1–657

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1hc1
Deposition date deposition_date	1991-05-15
Structure title title	CRYSTAL STRUCTURE OF HEXAMERIC HAEMOCYANIN FROM PANULIRUS INTERRUPTUS REFINED AT 3.2 ANGSTROMS RESOLUTION
Keywords keywords	OXYGEN TRANSPORT; OXYGEN TRANSPORT
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1hc1__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1hc1__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1hc1__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	48.59 Å
Rg (electron density)	47.58 Å
Total Rg	47.96 Å
Atom count	31050
Residues	3804
Excluded volume	545390 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1hc1__assembly_1__model_1`	hexameric （6）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	ARTHROPODAN HEMOCYANIN
Molecular weight formula_weight	75696.2 kDa
Source method src_method	man
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F
Atom count atom_count	32166

DALGTGNAQKQQDINHLLDKIYEPTKYPDLKDIAENFNPLGDTSIYNDHGAAVETLMKELNDHRLLEQRHWYSLFNTRQRKEALMLFAVLNQCKEWYCFRSNAAYFRERMNEGEFVYALYVSVIHSKLGDGIVLPPLYQITPHMFTNSEVIDKAYSAKMTQKPGTFNVSFTGTKKNREQRVAYFGEDIGMNIHHVTWHMDFPFWWEDSYGYHLDRKGELFFWVHHQLTARFDFERLSNWLDPVDELHWDRIIREGFAPLTSYKYGGEFPVRPDNIHFEDVDGVAHVHDLEITESRIHEAIDHGYITDSDGHTIDIRQPKGIELLGDIIESSKYSSNVQYYGSLHNTAHVMLGRQGDPHGKFNLPPGVMEHFETATRDPSFFRLHKYMDNIFKKHTDSFPPYTHDNLEFSGMVVNGVAIDGELITFFDEFQYSLINAVDSGENIEDVEINARVHRLNHNEFTYKITMSNNNDGERLATFRIFLCPIEDNNGITLTLDEARWFCIELDKFFQKVPSGPETIERSSKDSSVTVPDMPSFQSLKEQADNAVNGGHDLDLSAYERSCGIPDRMLLPKSKPEGMEFNLYVAVTDGDKDTEGHNGGHDYGGTHAQCGVHGEAYPDNRPLGYPLERRIPDERVIDGVSNIKHVVVKIVHHLEHHD

Source Source

Source organism organism	Panulirus interruptus
Common name common_name	California spiny lobster
NCBI taxonomy ID ncbi_taxonomy_id	6735

Entity 2 Entity 2 non-polymer

Description description	COPPER (II) ION
Molecular weight formula_weight	63.5 kDa
Source method src_method	syn
Copy count entity_count	12

Entity 3 Entity 3 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	1116

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6. Fold Classification (SCOP + CATH) 15 domains

SCOP 2.08 (3 domains)

Domain ID domain_id	d1hc1a1
Class class	a — All alpha proteins
Fold Fold fold	a.85 — Hemocyanin, N-terminal domain
Superfamily Superfamily superfamily	a.85.1 — Hemocyanin, N-terminal domain
Family Family family	a.85.1.1 — Hemocyanin, N-terminal domain

Domain ID domain_id	d1hc1a2
Class class	a — All alpha proteins
Fold Fold fold	a.86 — Di-copper centre-containing domain
Superfamily Superfamily superfamily	a.86.1 — Di-copper centre-containing domain
Family Family family	a.86.1.1 — Hemocyanin middle domain

Domain ID domain_id	d1hc1a3
Class class	b — All beta proteins
Fold Fold fold	b.1 — Immunoglobulin-like beta-sandwich
Superfamily Superfamily superfamily	b.1.18 — E set domains
Family Family family	b.1.18.3 — Arthropod hemocyanin, C-terminal domain

CATH v4.4 (12 domains)

Domain ID domain_id	1hc1A02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1280 — di-copper center containing domain from catechol oxidase
Homologous superfamily homologous superfamily	10 — Di-copper center containing domain from catechol oxidase

Domain ID domain_id	1hc1A03
Class class	2 — Mainly Beta
Architecture architecture	60 — Sandwich
Topology topology	40 — Immunoglobulin-like
Homologous superfamily homologous superfamily	1520 — Hemocyanin, C-terminal domain

Domain ID domain_id	1hc1B02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1280 — di-copper center containing domain from catechol oxidase
Homologous superfamily homologous superfamily	10 — Di-copper center containing domain from catechol oxidase

Domain ID domain_id	1hc1B03
Class class	2 — Mainly Beta
Architecture architecture	60 — Sandwich
Topology topology	40 — Immunoglobulin-like
Homologous superfamily homologous superfamily	1520 — Hemocyanin, C-terminal domain

Domain ID domain_id	1hc1C02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1280 — di-copper center containing domain from catechol oxidase
Homologous superfamily homologous superfamily	10 — Di-copper center containing domain from catechol oxidase

Domain ID domain_id	1hc1C03
Class class	2 — Mainly Beta
Architecture architecture	60 — Sandwich
Topology topology	40 — Immunoglobulin-like
Homologous superfamily homologous superfamily	1520 — Hemocyanin, C-terminal domain

Domain ID domain_id	1hc1D02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1280 — di-copper center containing domain from catechol oxidase
Homologous superfamily homologous superfamily	10 — Di-copper center containing domain from catechol oxidase

Domain ID domain_id	1hc1D03
Class class	2 — Mainly Beta
Architecture architecture	60 — Sandwich
Topology topology	40 — Immunoglobulin-like
Homologous superfamily homologous superfamily	1520 — Hemocyanin, C-terminal domain

Domain ID domain_id	1hc1E02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1280 — di-copper center containing domain from catechol oxidase
Homologous superfamily homologous superfamily	10 — Di-copper center containing domain from catechol oxidase

Domain ID domain_id	1hc1E03
Class class	2 — Mainly Beta
Architecture architecture	60 — Sandwich
Topology topology	40 — Immunoglobulin-like
Homologous superfamily homologous superfamily	1520 — Hemocyanin, C-terminal domain

Domain ID domain_id	1hc1F02
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1280 — di-copper center containing domain from catechol oxidase
Homologous superfamily homologous superfamily	10 — Di-copper center containing domain from catechol oxidase

Domain ID domain_id	1hc1F03
Class class	2 — Mainly Beta
Architecture architecture	60 — Sandwich
Topology topology	40 — Immunoglobulin-like
Homologous superfamily homologous superfamily	1520 — Hemocyanin, C-terminal domain

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7. Citations (8)

Crystal structure of hexameric haemocyanin from Panulirus interruptus refined at 3.2 A resolution.

J.Mol.Biol. (1989)

PMID: 2585484 · DOI: 10.1016/0022-2836(89)90276-3 PRIMARY

Spectroscopic Investigations of Panulirus Interruptus Hemocyanin in the Crystalline State

Eur.J.Biochem. (1989)

;Pseudo 2-Fold Symmetry in the Copper-Binding Domain of Arthropodan Haemocyanins. Possible Implications for the Evolution of Oxygen Transport Proteins ;

J.Mol.Biol. (1989)

;Panulirus Interruptus Hemocyanin. The Amino Acid Sequence of Subunit B and Anomalous Behavior of Subunits a and B on Polyacrylamide Gel Electrophoresis in the Presence of Sds ;

Eur.J.Biochem. (1988)

Panulirus Interruptus Hemocyanin. The Elucidation of the Complete Amino Acid Sequence of Subunit A

Eur.J.Biochem. (1987)

;Structure Determination of Panulirus Interruptus Haemocyanin at 3.2 Angstroms Resolution. Successful Phase Extension by Sixfold Density Averaging ;

J.Mol.Biol. (1986)

The Structure of Arthropod Hemocyanins

Science (1985)

3.2 Angstroms Structure of the Copper-Containing, Oxygen-Carrying Protein Panulirus Interruptus Haemocyanin

Nature (1984)

Unit-cell length a length_a	119.800 Å
Unit-cell length b length_b	193.100 Å
Unit-cell length c length_c	122.200 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	118.10 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	12

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	3.200 Å
Low-resolution limit resolution_low	8.000 Å
R factor r_factor	0.2010
R_work r_work	—
R_free r_free	—

CRYSTAL STRUCTURE OF HEXAMERIC HAEMOCYANIN FROM PANULIRUS INTERRUPTUS REFINED AT 3.2 ANGSTROMS RESOLUTION

1. Protein Identity and Related Structures Protein Identity & Related Structures

ARTHROPODAN HEMOCYANIN

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1hc1__assembly_1__model_1

1hc1__assembly_1__model_1 | I(q)

1hc1__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 water

6. Fold Classification (SCOP + CATH) 15 domains

SCOP 2.08 (3 domains)

CATH v4.4 (12 domains)

7. Citations (8)

CRYSTAL STRUCTURE OF HEXAMERIC HAEMOCYANIN FROM PANULIRUS INTERRUPTUS REFINED AT 3.2 ANGSTROMS RESOLUTION

1. Protein Identity and Related Structures Protein Identity & Related Structures

ARTHROPODAN HEMOCYANIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1hc1__assembly_1__model_1

1hc1__assembly_1__model_1 | I(q)

1hc1__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 water

6. Fold Classification (SCOP + CATH) 15 domains

SCOP 2.08 (3 domains)

CATH v4.4 (12 domains)

7. Citations (8)