ASTACIN
Astacus astacus
当前结构中的状态
| Assembly | 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与共同组分 | 实验方法与环境 | 结构质量 |
|---|---|---|---|---|---|---|
| 1 | 蛋白单体 单体 蛋白 × 1 PDB 声明：monomeric（1） 与蛋白拷贝数一致 | 链 A； UniProt 50–249 | 未记录 | HG MERCURY (II) ION × 1 | X-RAY DIFFRACTION mmCIF 未提供已读取环境条件 | 分辨率 2.10 Å |
数据库中的同蛋白其他状态
以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。
| 其他 PDB | 相对当前条目 1IAC | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法与环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1AST STRUCTURE OF ASTACIN AND IMPLICATIONS FOR ACTIVATION OF ASTACINS AND ZINC-LIGATION OF COLLAGENASES 提交 1993-04-21 | 配体/离子不同 结构质量不同 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric |
链 A
50–249（200 aa）
|
未记录 | ZN ZINC ION × 1 | X-RAY DIFFRACTION mmCIF 未提供已读取条件 | 分辨率 1.80 Å |
| 1IAA CRYSTAL STRUCTURES, SPECTROSCOPIC FEATURES, AND CATALYTIC PROPERTIES OF COBALT(II), COPPER(II), NICKEL(II), AND MERCURY(II) DERIVATIVES OF THE ZINC ENDOPEPTIDASE ASTACIN. A CORRELATION OF STRUCTURE AND PROTEOLYTIC ACTIVITY 提交 1994-05-09 | 配体/离子不同 结构质量不同 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric |
链 A
50–249（200 aa）
|
未记录 | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF 未提供已读取条件 | 分辨率 1.90 Å |
| 1IAB CRYSTAL STRUCTURES, SPECTROSCOPIC FEATURES, AND CATALYTIC PROPERTIES OF COBALT(II), COPPER(II), NICKEL(II), AND MERCURY(II) DERIVATIVES OF THE ZINC ENDOPEPTIDASE ASTACIN. A CORRELATION OF STRUCTURE AND PROTEOLYTIC ACTIVITY 提交 1994-05-09 | 配体/离子不同 结构质量不同 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric |
链 A
50–249（200 aa）
|
未记录 | CO COBALT (II) ION × 1 | X-RAY DIFFRACTION mmCIF 未提供已读取条件 | 分辨率 1.79 Å |
| 1IAD REFINED 1.8 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF ASTACIN, A ZINC-ENDOPEPTIDASE FROM THE CRAYFISH ASTACUS ASTACUS L. STRUCTURE DETERMINATION, REFINEMENT, MOLECULAR STRUCTURE AND COMPARISON TO THERMOLYSIN 提交 1994-05-09 | 配体/离子不同 结构质量不同 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric |
链 A
50–249（200 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION mmCIF 未提供已读取条件 | 分辨率 2.30 Å |
| 1IAE CRYSTAL STRUCTURES, SPECTROSCOPIC FEATURES, AND CATALYTIC PROPERTIES OF COBALT(II), COPPER(II), NICKEL(II), AND MERCURY(II) DERIVATIVES OF THE ZINC ENDOPEPTIDASE ASTACIN. A CORRELATION OF STRUCTURE AND PROTEOLYTIC ACTIVITY 提交 1994-05-09 | 配体/离子不同 结构质量不同 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric |
链 A
50–249（200 aa）
|
未记录 | NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION mmCIF 未提供已读取条件 | 分辨率 1.83 Å |
| 1QJI Structure of astacin with a transition-state analogue inhibitor 提交 1999-06-24 | 构建体不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric |
链 A
50–249（200 aa）
片段：CATALYTIC DOMAIN
|
未记录 | ZN ZINC ION × 1 PKF CARBOBENZOXY-PRO-LYS-PHE-Y(PO2)-ALA-PRO-OME × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；HANGING DROP VAPOUR DIFFUSION PH 7.0, 1M AMMONIUM SULFATE
|
分辨率 2.14 Å |
| 1QJJ Structure of astacin with a hydroxamic acid inhibitor 提交 1999-06-24 | 构建体不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 A
50–249（200 aa）
片段：CATALYTIC DOMAIN
|
未记录 | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；HANGING DROP VAPOUR DIFFUSION PH 7.0, 1M AMMONIUM SULFATE
|
分辨率 1.86 Å |
| 3LQ0 Zymogen structure of crayfish astacin metallopeptidase 提交 2010-02-08 | 构建体不同 突变/修饰不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric |
链 A
16–250（235 aa）
|
突变：I91L,E93A | ZN ZINC ION × 1 GOL GLYCEROL × 3 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；For crystallization, reservoir solutions were prepared by a Tecan robot and 200-nL crystallization drops were dispensed on 96x2-well MRC plates (Innovadyne) by a Cartesian (Genomic Solutions) nanodrop robot at the High-Throughput Crystallography Platform of the Barcelona Science Park. Best crystals appeared in a Bruker steady-temperature crystal farm at 4C with protein solution (10 mg/mL in 50mM AMPSO pH9.0) and 20% PEG 8000, 0.1M (NH4)2SO4, 0.01M MgCl2, 0.05M MES pH5.6 as reservoir solution. These conditions were efficiently scaled up to the microliter range with 24-well Cryschem crystallization dishes (Hampton Research). Crystals were cryo-protected with 16% PEG 8000, 20% glycerol, 0.1M (NH4)2SO4, 0.01M MgCl2, 0.05M MES pH5.6. , VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.45 Å R-free 0.180 |
| 6HT9 Mouse fetuin-B in complex with crayfish astacin 提交 2018-10-03 | 构建体不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 其他组合 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 A
1–251（251 aa）
|
未记录 | ZN ZINC ION × 1 GOL GLYCEROL × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；ammonium sulfate, polyethylene glycol 2000, sodium acetate, pH 4.6.
|
分辨率 3.10 Å R-free 0.270 |
| 6HT9 Mouse fetuin-B in complex with crayfish astacin 提交 2018-10-03 | 构建体不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 C
1–251（251 aa）
|
未记录 | ZN ZINC ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；ammonium sulfate, polyethylene glycol 2000, sodium acetate, pH 4.6.
|
分辨率 3.10 Å R-free 0.270 |
| 6SAZ Cleaved human fetuin-b in complex with crayfish astacin 提交 2019-07-18 | 构建体不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 1 其他组合 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 A
50–251（202 aa）
|
未记录 | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；Crystallization assays were set up following the sitting-drop vapor diffusion method at the joint IBMB/IRB Automated Crystallography Platform of Barcelona Science Park. A Tecan robot (Tecan Trading) was used to prepare reservoir solutions, and a Cartesian Microsys 4000 XL robot (Genomic Solutions) or a Phoenix nanodrop robot (Art Robbins Instruments) dispensed nanocrystallization drops on 96x2-well Swissci Polystyrene MRC Crystallization Plates (Molecular Dimensions). Plates were stored at 4 or 20 degrees in thermostatic crystal farms (Bruker AXS). The astacin-hFB complex only crystallized after incubating the inhibitor (at 7.5 mg/mL) with six-fold molar excess of the peptidase in 10 mM Tris-HCl, 140 mM sodium chloride, pH 6.8. Crystals were obtained at 20 degrees in 200 nL:100 nL drops with protein complex solution and 20 percent (w/v) polyethylene glycol 3,350, 0.2 M sodium tartrate dibasic as reservoir solution.
|
分辨率 3.00 Å R-free 0.247 |
| 6SAZ Cleaved human fetuin-b in complex with crayfish astacin 提交 2019-07-18 | 构建体不同 聚集状态不同 配体/离子不同 实验环境不同 结构质量不同 | Assembly 2 其他组合 异源复合物；蛋白 × 2 PDB 声明：dimeric |
链 C
50–251（202 aa）
|
未记录 | ZN ZINC ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；Crystallization assays were set up following the sitting-drop vapor diffusion method at the joint IBMB/IRB Automated Crystallography Platform of Barcelona Science Park. A Tecan robot (Tecan Trading) was used to prepare reservoir solutions, and a Cartesian Microsys 4000 XL robot (Genomic Solutions) or a Phoenix nanodrop robot (Art Robbins Instruments) dispensed nanocrystallization drops on 96x2-well Swissci Polystyrene MRC Crystallization Plates (Molecular Dimensions). Plates were stored at 4 or 20 degrees in thermostatic crystal farms (Bruker AXS). The astacin-hFB complex only crystallized after incubating the inhibitor (at 7.5 mg/mL) with six-fold molar excess of the peptidase in 10 mM Tris-HCl, 140 mM sodium chloride, pH 6.8. Crystals were obtained at 20 degrees in 200 nL:100 nL drops with protein complex solution and 20 percent (w/v) polyethylene glycol 3,350, 0.2 M sodium tartrate dibasic as reservoir solution.
|
分辨率 3.00 Å R-free 0.247 |
共 10 个其他 PDB 条目、12 个 assembly。 打开独立比较页并筛选聚集状态
查看构建体与数据证据
| UniProt名称 | ASTA_ASTFL |
| Isoform | — |
| PDB实体 | 1 |
| 链与序列区间 | 作者链 A； PDB构建体 1–200； UniProt 50–249 |
