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1pjt

The structure of the Ser128Ala point-mutant variant of CysG, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Siroheme synthase

Salmonella typhimurium

UniProt P25924

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein homooligomer Homooligomer Protein 2 S-ADENOSYL-L-HOMOCYSTEINE × 2 PHOSPHATE ION × 1 water × 2 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer
1pjq 1pjs 1pjt

3 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex

None

View Construct and Data Evidence
UniProt name CYSG_SALTY
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–457； UniProt 1–457 Author chain B； PDBConstruct 1–457； UniProt 1–457

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id1pjt
Deposition date deposition_date2003-06-03
Structure title titleThe structure of the Ser128Ala point-mutant variant of CysG, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis
Keywords keywordsrossmann fold, nucleotide binding motif, SAH, SAM, NAD, phosphoserine, TRANSFERASE-OXIDOREDUCTASE-LYASE COMPLEX; TRANSFERASE/OXIDOREDUCTASE/LYASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1pjt__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1pjt__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

1pjt__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）30.87 Å
Rg (electron density)29.95 Å
Total Rg30.57 Å
Atom count7028
Residues903
Excluded volume125120 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 1pjt__assembly_1__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

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6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (6 domains)

Domain ID domain_idd1pjta1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.2 — NAD(P)-binding Rossmann-fold domains
Superfamily Superfamily superfamilyc.2.1 — NAD(P)-binding Rossmann-fold domains
Family Family familyc.2.1.11 — Siroheme synthase N-terminal domain-like
Domain ID domain_idd1pjta2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.90 — Tetrapyrrole methylase
Superfamily Superfamily superfamilyc.90.1 — Tetrapyrrole methylase
Family Family familyc.90.1.1 — Tetrapyrrole methylase
Domain ID domain_idd1pjta3
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.37 — Siroheme synthase middle domains-like
Superfamily Superfamily superfamilye.37.1 — Siroheme synthase middle domains-like
Family Family familye.37.1.1 — Siroheme synthase middle domains-like
Domain ID domain_idd1pjtb1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.2 — NAD(P)-binding Rossmann-fold domains
Superfamily Superfamily superfamilyc.2.1 — NAD(P)-binding Rossmann-fold domains
Family Family familyc.2.1.11 — Siroheme synthase N-terminal domain-like
Domain ID domain_idd1pjtb2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.90 — Tetrapyrrole methylase
Superfamily Superfamily superfamilyc.90.1 — Tetrapyrrole methylase
Family Family familyc.90.1.1 — Tetrapyrrole methylase
Domain ID domain_idd1pjtb3
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.37 — Siroheme synthase middle domains-like
Superfamily Superfamily superfamilye.37.1 — Siroheme synthase middle domains-like
Family Family familye.37.1.1 — Siroheme synthase middle domains-like

CATH v4.4 (10 domains)

Domain ID domain_id1pjtA01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily720 — NAD(P)-binding Rossmann-like Domain
Domain ID domain_id1pjtA02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology160 — Double Stranded RNA Binding Domain
Homologous superfamily homologous superfamily110 — Sirohaem synthase, central domain
Domain ID domain_id1pjtA03
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology8 — Helicase, Ruva Protein; domain 3
Homologous superfamily homologous superfamily210 — Sirohaem synthase, dimerisation domain
Domain ID domain_id1pjtA04
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology1010 — Cobalt-precorrin-4 Transmethylase; domain 1
Homologous superfamily homologous superfamily10 — Tetrapyrrole methylase, N-terminal domain
Domain ID domain_id1pjtA05
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology950 — Methyltransferase, Cobalt-precorrin-4 Transmethylase; Domain 2
Homologous superfamily homologous superfamily10 — Tetrapyrrole methylase, C-terminal domain
Domain ID domain_id1pjtB01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily720 — NAD(P)-binding Rossmann-like Domain
Domain ID domain_id1pjtB02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology160 — Double Stranded RNA Binding Domain
Homologous superfamily homologous superfamily110 — Sirohaem synthase, central domain
Domain ID domain_id1pjtB03
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology8 — Helicase, Ruva Protein; domain 3
Homologous superfamily homologous superfamily210 — Sirohaem synthase, dimerisation domain
Domain ID domain_id1pjtB04
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology1010 — Cobalt-precorrin-4 Transmethylase; domain 1
Homologous superfamily homologous superfamily10 — Tetrapyrrole methylase, N-terminal domain
Domain ID domain_id1pjtB05
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology950 — Methyltransferase, Cobalt-precorrin-4 Transmethylase; Domain 2
Homologous superfamily homologous superfamily10 — Tetrapyrrole methylase, C-terminal domain
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7. Citations (1)