1rom

CRYSTAL STRUCTURE OF NITRIC REDUCTASE FROM DENITRIFYING FUNGUS FUSARIUM OXYSPORUM

Method: X-RAY DIFFRACTION D_max: 69.2 Å Quality: EXCELLENT

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

CYTOCHROME P450

物种未注明

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白单体单体蛋白 × 1 PDB 声明：monomeric（1）与蛋白拷贝数一致	链 A； UniProt 1–402	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件：pH 5.5；PROTEIN WAS CRYSTALLIZED FROM 100MM-MES BUFFER AT PH5.5 USING PEG4000 AS PRECIPITANT	分辨率 2.00 Å R-free 0.266

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 1ROM	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1CL6 CRYSTAL STRUCTURES OF FERRIC-NO COMPLEXES OF FUNGAL NITRIC OXIDE REDUCTASE AND ITS SER286 MUTANTS AT CRYOGENIC TEMPERATURE 提交 1999-05-06	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7；100MM-MES BUFFER AT PH 7.0 USING PEG 4K	分辨率 1.70 Å R-free 0.251
1CMJ CRYSTAL STRUCTURES OF FERRIC-NO COMPLEXES OF FUNGAL NITRIC OXIDE REDUCTASE AND THEIR SER286 MUTANTS AT CRYOGENIC TEMPERATURE 提交 1999-05-07	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	突变：S286T	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7；100MM-MES BUFFER AT PH 7.0 USING PEG4K	分辨率 1.70 Å R-free 0.250
1CMN CRYSTAL STRUCTURES OF FERRIC-NO COMPLEXES OF FUNGAL NITRIC OXIDE REDUCTASE AND THEIR SER286 MUTANTS AT CRYOGENIC TEMPERATURE 提交 1999-05-07	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	突变：S286V	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7；100MM-MES BUFFER AT PH 7.0 USING PEG4K	分辨率 1.70 Å R-free 0.228
1EHE CRYSTAL STRUCTURES OF CYTOCHROME P450NOR AND ITS MUTANTS (SER286 VAL, THR) IN THE FERRIC RESTING STATE AT CRYOGENIC TEMPERATURE: A COMPARATIVE ANALYSIS WITH MONOOXYGENASE CYTOCHROME P450S 提交 2000-02-21	实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；protein was crystallized from 100mM-Mes buffer, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 1.70 Å R-free 0.272
1EHF CRYSTAL STRUCTURES OF CYTOCHROME P450NOR AND ITS MUTANTS (SER286 VAL, THR) IN THE FERRIC RESTING STATE AT CRYOGENIC TEMPERATURE: A COMPARATIVE ANALYSIS WITH MONOOXYGENASE CYTOCHROME P450S 提交 2000-02-21	突变/修饰不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	突变：S286T	HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；protein was crystallized from 100mM-Mes buffer , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 1.70 Å R-free 0.265
1EHG CRYSTAL STRUCTURES OF CYTOCHROME P450NOR AND ITS MUTANTS (SER286 VAL, THR) IN THE FERRIC RESTING STATE AT CRYOGENIC TEMPERATURE: A COMPARATIVE ANALYSIS WITH MONOOXYGENASE CYTOCHROME P450S 提交 2000-02-21	突变/修饰不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	突变：S286V	HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；protein was crystallized from 100mM-Mes buffer, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 1.70 Å R-free 0.223
1F24 CRYSTAL STRUCTURE OF NO COMPLEX OF THR243ALA MUTANTS OF CYTOCHROME P450NOR 提交 2000-05-23	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	突变：T243A	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；PEG3350, MES, Glycerol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 1.40 Å R-free 0.207
1F25 CRYSTAL STRUCTURE OF NO COMPLEX OF THR243ASN MUTANTS OF CYTOCHROME P450NOR 提交 2000-05-23	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	突变：T243N	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；PEG3350, MES, Glycerol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 1.40 Å R-free 0.204
1F26 CRYSTAL STRUCTURE OF NO COMPLEX OF THR243VAL MUTANTS OF CYTOCHROME P450NOR 提交 2000-05-23	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	突变：T243V	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；PEG3350, MES, Glycerol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 1.40 Å R-free 0.207
1GED A positive charge route for the access of nadh to heme formed in the distal heme pocket of cytochrome p450nor 提交 2000-11-02	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）	未记录	BR BROMIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；PEG4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K	分辨率 2.00 Å R-free 0.279
1GEI STRUCTURAL CHARACTERIZATION OF N-BUTYL-ISOCYANIDE COMPLEXES OF CYTOCHROMES P450NOR AND P450CAM 提交 2000-11-13	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NBN N-BUTYL ISOCYANIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEG 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 1.60 Å R-free 0.258
1GEJ STRUCTURAL CHARACTERIZATION OF N-BUTYL-ISOCYANIDE COMPLEXES OF CYTOCHROMES P450NOR AND P450CAM 提交 2000-11-13	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NBN N-BUTYL ISOCYANIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；PEG4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 1.50 Å R-free 0.241
1JFB X-ray structure of nitric oxide reductase (cytochrome P450nor) in the ferric resting state at atomic resolution 提交 2001-06-20	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–404（404 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；PEG3350, MES, Glycerol, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 1.00 Å R-free 0.139
1JFC X-ray structure of nitric oxide reductase (cytochrome P450nor) in the ferrous CO state at atomic resolution 提交 2001-06-20	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–404（404 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CMO CARBON MONOXIDE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；PEG3350, MES, Glycerol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 1.05 Å R-free 0.152
1ULW Crystal structure of P450nor Ser73Gly/Ser75Gly mutant 提交 2003-09-16	突变/修饰不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	突变：S73G, S75G	HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.5；278 K；PEG8000, 0.2M sodium acetate, sodium cacodylate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K	分辨率 2.00 Å R-free 0.248
1XQD Crystal structure of P450NOR complexed with 3-pyridinealdehyde adenine dinucleotide 提交 2004-10-12	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 0–402（403 aa）	突变：S73G/S75G	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 DND NICOTINIC ACID ADENINE DINUCLEOTIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.5；278 K；PEG 8000, sodium acetate, sodium cacodylate, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 278K	分辨率 1.80 Å R-free 0.243
2ROM CRYSTAL STRUCTURE OF NITRIC REDUCTASE FROM DENITRIFYING FUNGUS FUSARIUM OXYSPORUM COMPLEX WITH CARBON MONOXIDE 提交 1997-03-24	配体/离子不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–402（402 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 CMO CARBON MONOXIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 5.5；PROTEIN WAS CRYSTALLIZED FROM 100MM-MES BUFFER AT PH5.5 USING PEG4000 AS PRECIPITANT	分辨率 2.00 Å R-free 0.269
5Y5F Structure of cytochrome P450nor in NO-bound state: damaged by low-dose (0.72 MGy) X-ray 提交 2017-08-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）片段：nitric-oxide reductase cytochrome P450	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.6；293 K；17% PEG 10000, 0.1 M BIS-TRIS 0.1 M Ammonium acetate	分辨率 1.50 Å R-free 0.186
5Y5G Structure of cytochrome P450nor in NO-bound state: damaged by high-dose (5.7 MGy) X-ray 提交 2017-08-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 6.6；293 K；17% PEG 10000, 0.1 M BIS-TRIS 0.1 M Ammonium acetate	分辨率 1.36 Å R-free 0.166
5Y5H SF-ROX structure of cytochrome P450nor (NO-bound state) determined at SACLA 提交 2017-08-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）片段：nitric-oxide reductase cytochrome P450	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 1.50 Å R-free 0.196
5Y5I Time-resolved SFX structure of cytochrome P450nor: 20 ms after photo-irradiation of caged NO in the presence of NADH (NO-bound state), light data 提交 2017-08-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）片段：nitric-oxide reductase cytochrome P450	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；34-38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 2.10 Å R-free 0.196
5Y5I Time-resolved SFX structure of cytochrome P450nor: 20 ms after photo-irradiation of caged NO in the presence of NADH (NO-bound state), light data 提交 2017-08-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 1–403（403 aa）片段：nitric-oxide reductase cytochrome P450	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1 GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；34-38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 2.10 Å R-free 0.196
5Y5J Time-resolved SFX structure of cytochrome P450nor: dark-2 data in the presence of NADH (resting state) 提交 2017-08-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；34-38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 2.00 Å R-free 0.197
5Y5J Time-resolved SFX structure of cytochrome P450nor: dark-2 data in the presence of NADH (resting state) 提交 2017-08-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；34-38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 2.00 Å R-free 0.197
5Y5K Time-resolved SFX structure of cytochrome P450nor : 20 ms after photo-irradiation of caged NO in the absence of NADH (NO-bound state), light data 提交 2017-08-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；34-38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 2.10 Å R-free 0.194
5Y5K Time-resolved SFX structure of cytochrome P450nor : 20 ms after photo-irradiation of caged NO in the absence of NADH (NO-bound state), light data 提交 2017-08-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；34-38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 2.10 Å R-free 0.194
5Y5L Time-resolved SFX structure of cytochrome P450nor: dark-2 data in the absence of NADH (resting state) 提交 2017-08-09	构建体不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；34-38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 2.10 Å R-free 0.193
5Y5L Time-resolved SFX structure of cytochrome P450nor: dark-2 data in the absence of NADH (resting state) 提交 2017-08-09	构建体不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；34-38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 2.10 Å R-free 0.193
5Y5M SFX structure of cytochrome P450nor: a complete dark data without pump laser (resting state) 提交 2017-08-09	构建体不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；34-38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 2.10 Å R-free 0.218
5Y5M SFX structure of cytochrome P450nor: a complete dark data without pump laser (resting state) 提交 2017-08-09	构建体不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；34-38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 2.10 Å R-free 0.218
7DVO Structure of Reaction Intermediate of Cytochrome P450 NO Reductase (P450nor) Determined by XFEL 提交 2021-01-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1 GOL GLYCEROL × 3	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 1.80 Å R-free 0.219
7DVO Structure of Reaction Intermediate of Cytochrome P450 NO Reductase (P450nor) Determined by XFEL 提交 2021-01-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 1–403（403 aa）	未记录	HEM PROTOPORPHYRIN IX CONTAINING FE × 1 NO NITRIC OXIDE × 1 GOL GLYCEROL × 3	X-RAY DIFFRACTION X-ray结晶条件 BATCH MODE；pH 8.5；293 K；38% PEG 10000, 0.1 M BIS-TRIS PROPANE 0.15 M Ammonium acetate	分辨率 1.80 Å R-free 0.219

共 26 个其他 PDB 条目、32 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	NOR_FUSOX
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 2–403； UniProt 1–402

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1rom
沉积日期 deposition_date	1997-03-24
结构标题 title	CRYSTAL STRUCTURE OF NITRIC REDUCTASE FROM DENITRIFYING FUNGUS FUSARIUM OXYSPORUM
关键词 keywords	NITRIC OXIDE REDUCTASE, CYTOCHROME P450NOR, OXIDOREDUCTASE; OXIDOREDUCTASE
实验方法 method	X-RAY DIFFRACTION

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	22.30 Å
回转半径 Rg (电子) rg_electron	21.21 Å
零角强度 I(0) i0	32499200.00
分子量 molecular_weight	44636.0 kDa
排除体积 excluded_volume	56237 Å³
包络体积 envelope_volume	65773 Å³
水化壳体积 shell_volume	25418 Å³
包络直径 envelope_diameter	70.8 Å
壳层 Rg shell_rg	28.30 Å
包络 Rg envelope_rg	21.22 Å
形状 Rg shape_rg	21.19 Å
总 Rg total_rg	22.14 Å
总原子数 total_atoms	3142
残基数 n_residues	399
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	69.2 Å
Rg (实空间) rg_real	22.17 Å
Rg 误差 (实空间) rg_real_error	0.31 Å
I(0) (实空间) i0_real	3.2500e+07
I(0) 误差 (实空间) i0_real_error	3.9390e+05
Rg (倒空间) rg_reciprocal	22.20 Å
I(0) (倒空间) i0_reciprocal	32500000.0000
解质量估计 total_estimate	0.9009
解质量评级 solution_quality	EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	28.3 Å
偏度 Skewness skewness	0.127
峰度 Kurtosis kurtosis	-0.493
角度范围 angular_range	— – 0.3550 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	5927000.0000
实空间数据点数 n_real_points	67
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.913; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.984; Smooth: 0.983

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (3)

实体 1 Entity 1 polymer

描述 description	CYTOCHROME P450
分子量 formula_weight	44420.7 kDa
EC 编号 ec_number	1.14.-.-
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	3301

MASGAPSFPFSRASGPEPPAEFAKLRATNPVSQVKLFDGSLAWLVTKHKDVCFVATSEKLSKVRTRQGFPELSASGKQAAKAKPTFVDMDPPEHMHQRSMVEPTFTPEAVKNLQPYIQRTVDDLLEQMKQKGCANGPVDLVKEFALPVPSYIIYTLLGVPFNDLEYLTQQNAIRTNGSSTAREASAANQELLDYLAILVEQRLVEPKDDIISKLCTEQVKPGNIDKSDAVQIAFLLLVAGNATMVNMIALGVATLAQHPDQLAQLKANPSLAPQFVEELCRYHTASALAIKRTAKEDVMIGDKLVRANEGIIASNQSANRDEEVFENPDEFNMNRKWPPQDPLGFGFGDHRCIAEHLAKAELTTVFSTLYQKFPDLKVAVPLGKINYTPLNRDVGIVDLPVIF

实体 2 Entity 2 non-polymer

描述 description	PROTOPORPHYRIN IX CONTAINING FE
分子量 formula_weight	616.5 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

实体 3 Entity 3 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	159

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7. 折叠分类 (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

结构域编号 domain_id	d1roma_
类 Class class	a — All alpha proteins
折叠类型 Fold fold	a.104 — Cytochrome P450
超家族 Superfamily superfamily	a.104.1 — Cytochrome P450
家族 Family family	a.104.1.1 — Cytochrome P450

CATH v4.4 (1 domains)

结构域编号 domain_id	1romA00
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	630 — Cytochrome p450
同源超家族 H-superfamily homologous superfamily	10 — Cytochrome P450

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8. 引用文献 (1)

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晶胞边长 a length_a	54.990 Å
晶胞边长 b length_b	82.660 Å
晶胞边长 c length_c	87.210 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	4

空间群 space_group	P 21 21 21
空间群编号 int_tables_number	19
晶系 crystal_system	—

分辨率 resolution	2.000 Å
最低分辨率 resolution_low	10.000 Å
R 因子 r_factor	0.1970
R_work r_work	0.1970
R_free r_free	0.2660

fit	/app/data/saxs_computation/ro/1rom/1rom.fit
pddf	/app/data/pr_computation/ro/1rom/1rom.pr.out
profile	/app/data/saxs_computation/ro/1rom/1rom.dat