1s4e

Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium

Method: X-RAY DIFFRACTION

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Galactokinase

Pyrococcus furiosus

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	蛋白单体	单体	蛋白 1	alpha-D-galactopyranose × 1 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	与蛋白数一致
2	蛋白单体	单体	蛋白 1	alpha-D-galactopyranose × 1 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	与蛋白数一致
3	蛋白单体	单体	蛋白 1	alpha-D-galactopyranose × 1 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	与蛋白数一致
4	蛋白单体	单体	蛋白 1	alpha-D-galactopyranose × 1 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	与蛋白数一致
5	蛋白单体	单体	蛋白 1	alpha-D-galactopyranose × 1 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	与蛋白数一致
6	蛋白单体	单体	蛋白 1	alpha-D-galactopyranose × 1 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	与蛋白数一致
7	蛋白单体	单体	蛋白 1	alpha-D-galactopyranose × 1 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	与蛋白数一致
8	蛋白单体	单体	蛋白 1	alpha-D-galactopyranose × 1 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	与蛋白数一致
9	蛋白单体	单体	蛋白 1	alpha-D-galactopyranose × 1 MAGNESIUM ION × 1 ADENOSINE-5'-DIPHOSPHATE × 1	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	GAL1_PYRFU
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–352； UniProt 1–352 作者链 B； PDB构建体 1–352； UniProt 1–352 作者链 C； PDB构建体 1–352； UniProt 1–352 作者链 D； PDB构建体 1–352； UniProt 1–352 作者链 E； PDB构建体 1–352； UniProt 1–352 作者链 F； PDB构建体 1–352； UniProt 1–352 作者链 G； PDB构建体 1–352； UniProt 1–352 作者链 H； PDB构建体 1–352； UniProt 1–352 作者链 I； PDB构建体 1–352； UniProt 1–352

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1s4e
沉积日期 deposition_date	2004-01-16
结构标题 title	Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium
关键词 keywords	GHMP kinase superfamily, P-loop, transferase; TRANSFERASE
实验方法 method	X-RAY DIFFRACTION

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3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

1s4e__assembly_7__model_1

Assembly 7 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

1s4e__assembly_7__model_1 | I(q)

100 个绘图点；横轴和纵轴均采用对数尺度。

1s4e__assembly_7__model_1 | P(r) · Pending

P(r) 将按相同 assembly/model 分别计算和展示。

Rg（Guinier）	18.91 Å
Rg（电子密度）	17.81 Å
总 Rg	18.67 Å
原子数	1851
残基数	252
排除体积	32582 Å³
最大 q	0.500 Å⁻¹

Assembly	Model	结构单元	聚集说明	状态	CRYSOL	操作
1	1	`1s4e__assembly_1__model_1`	monomeric （1）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
2	1	`1s4e__assembly_2__model_1`	monomeric （1）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
3	1	`1s4e__assembly_3__model_1`	monomeric （1）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
4	1	`1s4e__assembly_4__model_1`	monomeric （1）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
5	1	`1s4e__assembly_5__model_1`	monomeric （1）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
6	1	`1s4e__assembly_6__model_1`	monomeric （1）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
7	1	`1s4e__assembly_7__model_1`	monomeric （1）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
8	1	`1s4e__assembly_8__model_1`	monomeric （1）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
9	1	`1s4e__assembly_9__model_1`	monomeric （1）	成功	4.1.3-1-20251215 (887e7ef)	查看下载

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

描述 description	Galactokinase
分子量 formula_weight	39614.9 kDa
EC 编号 ec_number	2.7.1.6
来源方法 src_method	man
拷贝数 entity_count	9

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G,H,I
原子数 atom_count	22711

MSKITVKSPGRVNLIGEHTDYTYGYVMPMAIDLYTIITAEKYDKVQLYSEHFNEEKTFTLDNLTKEGSWIDYVKGVLWVLIQEGYKIGGLKGKITGDLPLGAGLSSSASFEVGILEVLNQLYNLNIDPLKKALLAKKAENEFVGVPCGILDQFAVVFGKKDNVIFLDTQTLQYEYIPFPKDVSVLVFYTGVKRELASSEYAERKRIAEESLRILGKESSKEVTEKDLGKLPPLHRKFFSYIVRENARVLEVRDALKEGDIEKVGKILTTAHWDLAENYRVSCEELDFFVKKAMELGAYGARLTGAGFGGSAIALVDKDKAKTIGDAILREYLAKFSWKAKYFVVKPSDGVGV

来源 Source

来源生物 organism	Pyrococcus furiosus
NCBI 分类编号 ncbi_taxonomy_id	2261
宿主生物 host_organism	Escherichia coli BL21(DE3)

实体 2 Entity 2 non-polymer

描述 description	alpha-D-galactopyranose
分子量 formula_weight	180.2 kDa
来源方法 src_method	man
拷贝数 entity_count	9

实体 3 Entity 3 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	9

实体 4 Entity 4 non-polymer

描述 description	ADENOSINE-5'-DIPHOSPHATE
分子量 formula_weight	427.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	9

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6. 折叠分类 (SCOP + CATH) 36 domains

SCOP 2.08 (18 domains)

结构域编号 domain_id	d1s4ea1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.5 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	d1s4ea2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.26 — GHMP Kinase, C-terminal domain
家族 Family family	d.58.26.7 — Galactokinase

结构域编号 domain_id	d1s4eb1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.5 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	d1s4eb2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.26 — GHMP Kinase, C-terminal domain
家族 Family family	d.58.26.7 — Galactokinase

结构域编号 domain_id	d1s4ec1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.5 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	d1s4ec2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.26 — GHMP Kinase, C-terminal domain
家族 Family family	d.58.26.7 — Galactokinase

结构域编号 domain_id	d1s4ed1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.5 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	d1s4ed2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.26 — GHMP Kinase, C-terminal domain
家族 Family family	d.58.26.7 — Galactokinase

结构域编号 domain_id	d1s4ee1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.5 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	d1s4ee2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.26 — GHMP Kinase, C-terminal domain
家族 Family family	d.58.26.7 — Galactokinase

结构域编号 domain_id	d1s4ef1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.5 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	d1s4ef2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.26 — GHMP Kinase, C-terminal domain
家族 Family family	d.58.26.7 — Galactokinase

结构域编号 domain_id	d1s4eg1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.5 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	d1s4eg2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.26 — GHMP Kinase, C-terminal domain
家族 Family family	d.58.26.7 — Galactokinase

结构域编号 domain_id	d1s4eh1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.5 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	d1s4eh2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.26 — GHMP Kinase, C-terminal domain
家族 Family family	d.58.26.7 — Galactokinase

结构域编号 domain_id	d1s4ei1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.14 — Ribosomal protein S5 domain 2-like
超家族 Superfamily superfamily	d.14.1 — Ribosomal protein S5 domain 2-like
家族 Family family	d.14.1.5 — GHMP Kinase, N-terminal domain

结构域编号 domain_id	d1s4ei2
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.26 — GHMP Kinase, C-terminal domain
家族 Family family	d.58.26.7 — Galactokinase

CATH v4.4 (18 domains)

结构域编号 domain_id	1s4eA01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1s4eA02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	890 — GHMP kinase, C-terminal domain

结构域编号 domain_id	1s4eB01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1s4eB02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	890 — GHMP kinase, C-terminal domain

结构域编号 domain_id	1s4eC01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1s4eC02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	890 — GHMP kinase, C-terminal domain

结构域编号 domain_id	1s4eD01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1s4eD02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	890 — GHMP kinase, C-terminal domain

结构域编号 domain_id	1s4eE01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1s4eE02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	890 — GHMP kinase, C-terminal domain

结构域编号 domain_id	1s4eF01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1s4eF02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	890 — GHMP kinase, C-terminal domain

结构域编号 domain_id	1s4eG01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1s4eG02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	890 — GHMP kinase, C-terminal domain

结构域编号 domain_id	1s4eH01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1s4eH02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	890 — GHMP kinase, C-terminal domain

结构域编号 domain_id	1s4eI01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	230 — Ribosomal Protein S5; domain 2
同源超家族 H-superfamily homologous superfamily	10 —

结构域编号 domain_id	1s4eI02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	890 — GHMP kinase, C-terminal domain

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晶胞边长 a length_a	211.974 Å
晶胞边长 b length_b	355.672 Å
晶胞边长 c length_c	165.613 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	72

空间群 space_group	C 2 2 21
空间群编号 int_tables_number	20
晶系 crystal_system	—

分辨率 resolution	2.900 Å
最低分辨率 resolution_low	10.000 Å
R 因子 r_factor	0.2321
R_work r_work	0.2302
R_free r_free	0.2697

Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Galactokinase

当前结构中的状态

数据库中的同蛋白其他状态

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1s4e__assembly_7__model_1

1s4e__assembly_7__model_1 | I(q)

1s4e__assembly_7__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 non-polymer

6. 折叠分类 (SCOP + CATH) 36 domains

SCOP 2.08 (18 domains)

CATH v4.4 (18 domains)

7. 引用文献 (1)

Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Galactokinase

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1s4e__assembly_7__model_1

1s4e__assembly_7__model_1 | I(q)

1s4e__assembly_7__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 non-polymer

6. 折叠分类 (SCOP + CATH) 36 domains

SCOP 2.08 (18 domains)

CATH v4.4 (18 domains)

7. 引用文献 (1)