Aspartate aminotransferase
Escherichia coli
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count | Chain A； UniProt 1–396 | Mutation：V39L,K41Y,T47I,N69L,T109S,A293D,N297S Non-standard monomer：Yes (specific site not provided by mmCIF) | HCI HYDROCINNAMIC ACID × 2 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7.5；294 K；potassium phosphate, PLP, EDTA, DTT, PEG 400, N-methylmorpholine, ammonium sulfate, hydrocinnamic acid, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K | Resolution 1.90 Å R-free 0.197 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 1TOI | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AAM THE STRUCTURAL BASIS FOR THE ALTERED SUBSTRATE SPECIFICITY OF THE R292D ACTIVE SITE MUTANT OF ASPARTATE AMINOTRANSFERASE FROM E. COLI Deposited 1993-07-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：R280D Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SUBTILISIN CRYSTALS WERE CROSS-LINKED WITH GLUTARALDEHYDE
AND PLACED IN ANHYDROUS ACETONITRILE PRIOR TO DATA
COLLECTION.
|
Resolution 2.80 Å |
| 1AAW THE STRUCTURAL BASIS FOR THE ALTERED SUBSTRATE SPECIFICITY OF THE R292D ACTIVE SITE MUTANT OF ASPARTATE AMINOTRANSFERASE FROM E. COLI Deposited 1993-07-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.40 Å |
| 1AHE ASPARTATE AMINOTRANSFERASE HEXAMUTANT Deposited 1995-02-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：V39L, K41Y, T47I, N69L, T109S, N297S Mutation：V39L, K41Y, T47I, N69L, T109S, N297S | SO4 SULFATE ION × 2 PLP PYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 1AHF ASPARTATE AMINOTRANSFERASE HEXAMUTANT Deposited 1995-02-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：V39L, K41Y, T47I, N69L, T109S, N297S Mutation：V39L, K41Y, T47I, N69L, T109S, N297S | PLP PYRIDOXAL-5'-PHOSPHATE × 2 IOP INDOLYLPROPIONIC ACID × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 1AHG ASPARTATE AMINOTRANSFERASE HEXAMUTANT Deposited 1995-02-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: tetrameric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Not recorded | TYR TYROSINE × 2 PLP PYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 1AHX ASPARTATE AMINOTRANSFERASE HEXAMUTANT Deposited 1995-02-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：V39L, K41Y, T47I, N69L, T109S, N297S Mutation：V39L, K41Y, T47I, N69L, T109S, N297S | PLP PYRIDOXAL-5'-PHOSPHATE × 2 HCI HYDROCINNAMIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1AHY ASPARTATE AMINOTRANSFERASE HEXAMUTANT Deposited 1995-02-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：V39L, K41Y, T47I, N69L, T109S, N297S Mutation：V39L, K41Y, T47I, N69L, T109S, N297S | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 1AIA STRUCTURAL BASIS FOR THE CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE K258H LACKING THE PYRIDOXAL-5'-PHOSPHATE BINDING LYSINE RESIDUE Deposited 1994-05-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：K246H Mutation：K246H | PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1AIB STRUCTURAL BASIS FOR THE CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE K258H LACKING THE PYRIDOXAL-5'-PHOSPHATE BINDING LYSINE RESIDUE Deposited 1994-05-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：K246H Mutation：K246H | PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 AKG 2-OXOGLUTARIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1AIC STRUCTURAL BASIS FOR THE CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE K258H LACKING THE PYRIDOXAL-5'-PHOSPHATE BINDING LYSINE RESIDUE Deposited 1994-05-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：K246H Mutation：K246H | SO4 SULFATE ION × 2 PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.40 Å |
| 1AMQ X-RAY CRYSTALLOGRAPHIC STUDY OF PYRIDOXAMINE 5'-PHOSPHATE-TYPE ASPARTATE AMINOTRANSFERASES FROM ESCHERICHIA COLI IN THREE FORMS Deposited 1994-07-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1AMR X-RAY CRYSTALLOGRAPHIC STUDY OF PYRIDOXAMINE 5'-PHOSPHATE-TYPE ASPARTATE AMINOTRANSFERASES FROM ESCHERICHIA COLI IN THREE FORMS Deposited 1994-07-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 1AMS X-RAY CRYSTALLOGRAPHIC STUDY OF PYRIDOXAMINE 5'-PHOSPHATE-TYPE ASPARTATE AMINOTRANSFERASES FROM ESCHERICHIA COLI IN THREE FORMS Deposited 1994-07-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 GUA GLUTARIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.70 Å |
| 1ARG Aspartate aminotransferase, phospho-5'-pyridoxyl aspartate complex Deposited 1995-08-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Not recorded | PPD 2-[(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYLENE)-AMINO]-SUCCINIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.20 Å |
| 1ARH ASPARTATE AMINOTRANSFERASE, Y225R/R386A MUTANT Deposited 1995-08-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：Y225R, R386A Mutation：Y225R, R386A | PPD 2-[(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYLENE)-AMINO]-SUCCINIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.30 Å |
| 1ARI Aspartate aminotransferase, W140H mutant, maleate complex Deposited 1995-08-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：W140H Mutation：W140H | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.30 Å |
| 1ARS X-RAY CRYSTALLOGRAPHIC STUDY OF PYRIDOXAL 5'-PHOSPHATE-TYPE ASPARTATE AMINOTRANSFERASES FROM ESCHERICHIA COLI IN OPEN AND CLOSED FORM Deposited 1993-08-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 1ART X-RAY CRYSTALLOGRAPHIC STUDY OF PYRIDOXAL 5'-PHOSPHATE-TYPE ASPARTATE AMINOTRANSFERASES FROM ESCHERICHIA COLI IN OPEN AND CLOSED FORM Deposited 1993-08-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 0A0 2-methyl-L-aspartic acid × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 1ASA THE STRUCTURAL BASIS FOR THE REDUCED ACTIVITY OF THE Y226F(Y225F) ACTIVE SITE MUTANT OF E. COLI ASPARTATE AMINOTRANSFERASE Deposited 1993-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.40 Å |
| 1ASB THE STRUCTURAL BASIS FOR THE REDUCED ACTIVITY OF THE D223A(D222A) ACTIVE SITE MUTANT OF E. COLI ASPARTATE AMINOTRANSFERASE Deposited 1993-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：D211A | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1ASC THE STRUCTURAL BASIS FOR THE REDUCED ACTIVITY OF THE D223A(D222A) ACTIVE SITE MUTANT OF E. COLI ASPARTATE AMINOTRANSFERASE Deposited 1993-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：D211A | NPL N-METHYL-4-DEOXY-4-AMINO-PYRIDOXAL-5-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.40 Å |
| 1ASD THE STRUCTURE OF WILD TYPE E. COLI ASPARTATE AMINOTRANSFERASE RECONSTITUTED WITH N-MEPLP Deposited 1993-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | MPL N-METHYL-PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1ASE THE STRUCTURE OF WILD TYPE E. COLI ASPARTATE AMINOTRANSFERASE RECONSTITUTED WITH PLP-N-OXIDE Deposited 1993-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | NOP PYRIDOXAL-5'-PHOSPHATE-N-OXIDE × 2 MAE MALEIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 1ASF THE STRUCTURAL BASIS FOR THE REDUCED ACTIVITY OF THE Y226F(Y225F) ACTIVE SITE MUTANT OF E. COLI ASPARTATE AMINOTRANSFERASE Deposited 1993-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：Y214F | SO4 SULFATE ION × 2 PLP PYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1ASG THE STRUCTURAL BASIS FOR THE REDUCED ACTIVITY OF THE Y226F(Y225F) ACTIVE SITE MUTANT OF E. COLI ASPARTATE AMINOTRANSFERASE Deposited 1993-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：Y214F | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1ASL CRYSTAL STRUCTURES OF ESCHERICHIA COLI ASPARTATE AMINOTRANSFERASE IN TWO CONFORMATIONS: COMPARISON OF AN UNLIGANDED OPEN AND TWO LIGANDED CLOSED FORMS Deposited 1993-09-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Not recorded | PLA 2-[(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL)-AMINO]-2-METHYL-SUCCINIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1ASM CRYSTAL STRUCTURES OF ESCHERICHIA COLI ASPARTATE AMINOTRANSFERASE IN TWO CONFORMATIONS: COMPARISON OF AN UNLIGANDED OPEN AND TWO LIGANDED CLOSED FORMS Deposited 1993-09-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.35 Å |
| 1ASN CRYSTAL STRUCTURES OF ESCHERICHIA COLI ASPARTATE AMINOTRANSFERASE IN TWO CONFORMATIONS: COMPARISON OF AN UNLIGANDED OPEN AND TWO LIGANDED CLOSED FORMS Deposited 1993-09-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Not recorded | SO4 SULFATE ION × 2 PLP PYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.50 Å |
| 1B4X ASPARTATE AMINOTRANSFERASE FROM E. COLI, C191S MUTATION, WITH BOUND MALEATE Deposited 1998-12-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Fragment：COMPLETE SUBUNIT
|
Mutation：YES | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 2.45 Å |
| 1BQA ASPARTATE AMINOTRANSFERASE P195A MUTANT Deposited 1998-08-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：P195A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P195A Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.10 Å R-free 0.230 |
| 1BQD ASPARTATE AMINOTRANSFERASE P138A/P195A DOUBLE MUTANT Deposited 1998-08-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：P138A, P195A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：P138A, P195A Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.10 Å R-free 0.240 |
| 1C9C ASPARTATE AMINOTRANSFERASE COMPLEXED WITH C3-PYRIDOXAL-5'-PHOSPHATE Deposited 1999-08-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PP3 ALANYL-PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；ammonium sulfate, potassium phosphate, C3-pyridoxal-5p-phosphate, sodium azide, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.269 |
| 1CQ6 ASPARTATE AMINOTRANSFERASE COMPLEX WITH C4-PYRIDOXAL-5P-PHOSPHATE Deposited 1999-08-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PY4 2-[O-PHOSPHONOPYRIDOXYL]-AMINO- BUTYRIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；ammonium sulfate, potassium phosphate, C4-pyridoxal-5p-phosphate, sodium azide , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.70 Å R-free 0.297 |
| 1CQ7 ASPARTATE AMINOTRANSFERASE (E.C. 2.6.1.1) COMPLEXED WITH C5-PYRIDOXAL-5P-PHOSPHATE Deposited 1999-08-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PY5 2-[O-PHOSPHONOPYRIDOXYL]-AMINO-PENTANOIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；ammonium sulfate, potassium phosphate, C4-pyridoxal-5p-phosphate,sodium azide , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.251 |
| 1CQ8 ASPARTATE AMINOTRANSFERASE (E.C. 2.6.1.1) COMPLEXED WITH C6-PYRIDOXAL-5P-PHOSPHATE Deposited 1999-08-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PY6 2-[O-PHOSPHONOPYRIDOXYL]-AMINO-HEXANOIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；ammonium sulfate, potassium phosphate, C6-pyridoxal-5p-phosphate,sodium azide, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.270 |
| 1CZC ASPARTATE AMINOTRANSFERASE MUTANT ATB17/139S/142N WITH GLUTARIC ACID Deposited 1999-09-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T,F24L,N34D,I37M,K41N,K126R,A269T,A293V, N297S,S311G,I353T,S361F,S363G, V387L,M397L | PLP PYRIDOXAL-5'-PHOSPHATE × 2 GUA GLUTARIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PROTEIN WAS CRYSTALLIZED FROM 1.7M AMMONIUM SULFATE, 0.1 M SODIUM HEPES, 0.167
M GLUTARIC ACID, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
|
Resolution 2.50 Å R-free 0.243 |
| 1CZE ASPARTATE AMINOTRANSFERASE MUTANT ATB17/139S/142N WITH SUCCINIC ACID Deposited 1999-09-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T,F24L,N34D,I37M,K41N,K126R,A269T,A293V, N297S,S311G,I353T,S361F,S363G, V387L,M397L | PLP PYRIDOXAL-5'-PHOSPHATE × 2 SIN SUCCINIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PROTEIN WAS CRYSTALLIZED FROM 1.7M AMMONIUM SULFATE, 0.1 M SODIUM HEPES, 0.167
M SUCCINIC ACID, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
|
Resolution 2.40 Å R-free 0.244 |
| 1G4V ASPARTATE AMINOTRANSFERASE ACTIVE SITE MUTANT N194A/Y225F Deposited 2000-10-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：N194A, Y225F | PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Sodium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 20.0K
|
Resolution 2.00 Å |
| 1G4X ASPARTATE AMINOTRANSFERASE ACTIVE SITE MUTANT N194A/R292L Deposited 2000-10-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：N194A, R292L | PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Sodium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å |
| 1G7W ASPARTATE AMINOTRANSFERASE ACTIVE SITE MUTANT N194A/R386L Deposited 2000-11-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：N194A, R386L | PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Sodium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å |
| 1G7X ASPARTATE AMINOTRANSFERASE ACTIVE SITE MUTANT N194A/R292L/R386L Deposited 2000-11-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：N194A, R292L, R386L | PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Sodium Sulfate , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å |
| 1IX6 Aspartate Aminotransferase Active Site Mutant V39F Deposited 2002-06-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：V39F | PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Ammonium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.20 Å R-free 0.258 |
| 1IX7 Aspartate Aminotransferase Active Site Mutant V39F maleate complex Deposited 2002-06-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：V39F | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Ammonium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.247 |
| 1IX8 Aspartate Aminotransferase Active Site Mutant V39F/N194A Deposited 2002-06-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：V39F/N194A | PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Ammonium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.259 |
| 1QIR ASPARTATE AMINOTRANSFERASE FROM ESCHERICHIA COLI, C191Y MUTATION, WITH BOUND MALEATE Deposited 1999-06-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Fragment：COMPLETE SUBUNIT
|
Mutation：YES | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；PROTEIN SOLUTION: 6MG/ML PROTEIN, 20 MM POTASSIUM PHOSPHATE BUFFER, PH 7.5, 10 UM PLP, 5 MM EDTA, RESERVOIR SOLUTION: 20MM POTASSIUM PHOSPHATE BUFFER, PH 7.5, AND 45-50% AMMONIUM SULFATE
|
Resolution 2.20 Å |
| 1QIS ASPARTATE AMINOTRANSFERASE FROM ESCHERICHIA COLI, C191F MUTATION, WITH BOUND MALEATE Deposited 1999-06-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Fragment：COMPLETE SUBUNIT
|
Mutation：YES | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；PROTEIN SOLUTION: 13MG/ML PROTEIN, 20 MM POTASSIUM PHOSPHATE BUFFER, PH 7.5, 10 UM PLP, 5 MM EDTA, RESERVOIR SOLUTION: 20MM POTASSIUM PHOSPHATE BUFFER, PH 7.5, AND 45-50% AMMONIUM SULFATE
|
Resolution 1.90 Å |
| 1QIT ASPARTATE AMINOTRANSFERASE FROM ESCHERICHIA COLI, C191W MUTATION, WITH BOUND MALEATE Deposited 1999-06-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Fragment：COMPLETE SUBUNIT
|
Mutation：YES | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；PROTEIN SOLUTION: 9MG/ML PROTEIN, 20 MM POTASSIUM PHOSPHATE BUFFER, PH 7.5, 10 UM PLP, 5 MM EDTA, RESERVOIR SOLUTION: 20MM POTASSIUM PHOSPHATE BUFFER, PH 7.5, AND 45-50% AMMONIUM SULFATE
|
Resolution 1.90 Å |
| 1SPA ROLE OF ASP222 IN THE CATALYTIC MECHANISM OF ESCHERICHIA COLI ASPARTATE AMINOTRANSFERASE: THE AMINO ACID RESIDUE WHICH ENHANCES THE FUNCTION OF THE ENZYME-BOUND COENZYME PYRIDOXAL 5'-PHOSPHATE Deposited 1993-01-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | NPL N-METHYL-4-DEOXY-4-AMINO-PYRIDOXAL-5-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1TOE Unliganded structure of Hexamutant + A293D mutant of E. coli aspartate aminotransferase Deposited 2004-06-14 | Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：V39L,K41Y,T47I,N69L,T109S,A293D,N297S Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；294 K；potassium phosphate, PLP, EDTA, DTT, PEG 400, N-methylmorpholine, ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 2.00 Å R-free 0.202 |
| 1TOG Hydrocinnamic acid-bound structure of SRHEPT + A293D mutant of E. coli aspartate aminotransferase Deposited 2004-06-14 | Different construct Different mutation/modification Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：A12T,P13T,N34D,T109S,G261A,S285G,A293D,N297S Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：A12T,P13T,N34D,T109S,G261A,S285G,A293D,N297S Non-standard monomer：Yes (specific site not provided by mmCIF) | HCI HYDROCINNAMIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；294 K；potassium phosphate, PLP, EDTA, DTT, PEG 400, N-methylmorpholine, ammonium sulfate, hydrocinnamic acid, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 2.31 Å R-free 0.232 |
| 1TOJ Hydrocinnamic acid-bound structure of SRHEPT mutant of E. coli aspartate aminotransferase Deposited 2004-06-14 | Different mutation/modification Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A12T,P13T,N34D,T109S,G261A,S285G,N297S Non-standard monomer：Yes (specific site not provided by mmCIF) | HCI HYDROCINNAMIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；294 K；potassium phosphate, PLP, EDTA, DTT, PEG 400, N-methylmorpholine, ammonium sulfate, hydrocinnamic acid, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.90 Å R-free 0.201 |
| 1TOK Maleic acid-bound structure of SRHEPT mutant of E. coli aspartate aminotransferase Deposited 2004-06-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Mutation：A12T,P13T,N34D,T109S,G261A,S285G,N297S Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：A12T,P13T,N34D,T109S,G261A,S285G,N297S Non-standard monomer：Yes (specific site not provided by mmCIF) | MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；294 K；potassium phosphate, PLP, EDTA, DTT, PEG 400, N-methylmorpholine, ammonium sulfate, maleic acid, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 1.85 Å R-free 0.227 |
| 1X28 Crystal Structure of e.coli AspAT complexed with N-phosphopyridoxyl-L-glutamic acid Deposited 2005-04-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Not recorded | PGU N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG 400, Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.243 |
| 1X29 Crystal Structure of e.coli AspAT complexed with N-phosphopyridoxyl-2-methyl-L-glutamic acid Deposited 2005-04-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Not recorded | PMG N-({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)-2-METHYL-L-GLUTAMIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG 400, Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.232 |
| 1X2A Crystal Structure of e.coli AspAT complexed with N-phosphopyridoxyl-D-glutamic acid Deposited 2005-04-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain B
1–396（396 aa）
|
Not recorded | PDG N-({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)-D-GLUTAMIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；PEG 400, Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.234 |
| 1YOO ASPARTATE AMINOTRANSFERASE MUTANT ATB17 WITH ISOVALERIC ACID Deposited 1998-06-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T, F24L, N34D, I37M, K41N, K126R, S139G, N142T, A269T, A293V, N297S, S311G, I353T, S361F, S363G, V387L, M397L | PLP PYRIDOXAL-5'-PHOSPHATE × 2 IVA ISOVALERIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.40 Å R-free 0.235 |
| 2AAT 2.8-ANGSTROMS-RESOLUTION CRYSTAL STRUCTURE OF AN ACTIVE-SITE MUTANT OF ASPARTATE AMINOTRANSFERASE FROM ESCHERICHIA COLI Deposited 1989-05-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：K246A | SO4 SULFATE ION × 2 PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 2D5Y Aspartate Aminotransferase Mutant MC With Isovaleric Acid Deposited 2005-11-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T, A293V, N297S, I353T, S361F, S363G, V387L, M397L | PLP PYRIDOXAL-5'-PHOSPHATE × 2 IVA ISOVALERIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；35% Na2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.98 Å R-free 0.216 |
| 2D61 Aspartate Aminotransferase Mutant MA With Maleic Acid Deposited 2005-11-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T, F24L, N34D, I37M, K41N, A293V, N297S | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；35% Na2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.01 Å R-free 0.221 |
| 2D63 Aspartate Aminotransferase Mutant MA With Isovaleric Acid Deposited 2005-11-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T, F24L, N34D, I37M, K41N, A293V, N297S | PLP PYRIDOXAL-5'-PHOSPHATE × 2 IVA ISOVALERIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；35% Na2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.05 Å R-free 0.216 |
| 2D64 Aspartate Aminotransferase Mutant MABC With Isovaleric Acid Deposited 2005-11-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T, F24L, N34D, I37M, K41N, S139G, N142T, A293V, N297S, I353T, S361F, S363G, V387L, M397L | PLP PYRIDOXAL-5'-PHOSPHATE × 2 IVA ISOVALERIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；35% Na2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.05 Å R-free 0.231 |
| 2D65 Aspartate Aminotransferase Mutant MABC Deposited 2005-11-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T, F24L, N34D, I37M, K41N, S139G, N142T, A293V, N297S, I353T, S361F, S363G, V387L, M397L | SO4 SULFATE ION × 2 PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；35% Na2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.202 |
| 2D66 Aspartate Aminotransferase Mutant MAB Deposited 2005-11-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T, F24L, N34D, I37M, K41N, S139G, N142T, A293V, N297S | SO4 SULFATE ION × 2 PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；35% Na2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.18 Å R-free 0.216 |
| 2D7Y Aspartate Aminotransferase Mutant MA Deposited 2005-11-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T, F24L, N34D, I37M, K41N, A293V, N297S | PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；35% Na2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.66 Å R-free 0.215 |
| 2D7Z Aspartate Aminotransferase Mutant MAB Complexed with Maleic Acid Deposited 2005-11-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：A11T, F24L, N34D, I37M, K41N, S139G, N142T, A293V, N297S | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；35% Na2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.65 Å R-free 0.219 |
| 2Q7W Structural Studies Reveals the Inactivation of E. coli L-aspartate aminotransferase (S)-4,5-amino-dihydro-2-thiophenecarboxylic acid (SADTA) via two mechanisms at pH 6.0 Deposited 2007-06-07 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 2 PSZ 4-[({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)AMINO]THIOPHENE-2-CARBOXYLIC ACID × 2 PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 GOL GLYCEROL × 22 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6；298 K；25 mM potassium phosphate, 43% saturated ammonium sulfate, 20 mM SADTA, pH 6.0, EVAPORATION, temperature 298K
|
Resolution 1.40 Å R-free 0.188 |
| 2QA3 Structural Studies Reveal the Inactivation of E. coli L-aspartate aminotransferase by (S)-4,5-amino-dihydro-2-thiophenecarboxylic acid (SADTA) via two mechanisms (at pH6.5) Deposited 2007-06-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 6 PSZ 4-[({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)AMINO]THIOPHENE-2-CARBOXYLIC ACID × 2 PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 GOL GLYCEROL × 26 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 6.5；298 K；The well solutions contained 25 mM potassium phosphate and 43% saturated ammonium sulfate with 20 mM of SADTA at pH 6.5, EVAPORATION, temperature 298K
|
Resolution 1.75 Å R-free 0.197 |
| 2QB2 Structural Studies Reveal the Inactivation of E. coli L-aspartate aminotransferase by (s)-4,5-dihydro-2thiophenecarboylic acid (SADTA) via two mechanisms (at pH 7.0). Deposited 2007-06-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 4 PSZ 4-[({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)AMINO]THIOPHENE-2-CARBOXYLIC ACID × 2 PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 GOL GLYCEROL × 24 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 7；298 K；The well solutions contained 25 mM potassium phosphate and 43% saturated ammonium sulfate with 20 mM of SADTA at pH 7.0, EVAPORATION, temperature 298K
|
Resolution 1.70 Å R-free 0.184 |
| 2QB3 Structural Studies Reveal the Inactivation of E. coli L-Aspartate Aminotransferase by (s)-4,5-dihydro-2-thiophenecarboxylic acid (SADTA) via Two Mechanisms (at pH 7.5) Deposited 2007-06-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 4 PSZ 4-[({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)AMINO]THIOPHENE-2-CARBOXYLIC ACID × 2 PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 GOL GLYCEROL × 14 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 7.5；298 K；The well solutions contained 25 mM potassium phosphate and 43% saturated ammonium sulfate with 20 mM of SADTA at pH 7.5, EVAPORATION, temperature 298K
|
Resolution 1.45 Å R-free 0.178 |
| 2QBT Structural Studies Reveal The Inactivation of E. coli L-aspartate aminotransferase by (S)-4,5-amino-dihydro-2-thiophenecarboxylic acid (SADTA) via Two Mechanisms (at pH 8.0) Deposited 2007-06-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 6 PSZ 4-[({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)AMINO]THIOPHENE-2-CARBOXYLIC ACID × 2 PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 GOL GLYCEROL × 16 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 8；298 K；25 mM potassium phosphate, 43% saturated ammonium sulfate, 20 mM SADTA, pH 8.0, EVAPORATION, temperature 298K
|
Resolution 1.75 Å R-free 0.190 |
| 3AAT ACTIVITY AND STRUCTURE OF THE ACTIVE-SITE MUTANTS R386Y AND R386F OF ESCHERICHIA COLI ASPARTATE AMINOTRANSFERASE Deposited 1990-12-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：R374F | SO4 SULFATE ION × 2 PLP PYRIDOXAL-5'-PHOSPHATE × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 3QN6 Crystal Structures of Escherichia coli Aspartate Aminotransferase Reconstituted with 1-Deaza-Pyridoxal 5'-Phosphate: Internal Aldimine and Stable L-Aspartate External Aldimine Deposited 2011-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 10 EDO 1,2-ETHANEDIOL × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 L of protein (15-20 mg/ml, 50 mM TEA, pH 7.5, 100 mM KCL, 2 mM DTT, 10 M deaza-PLP) mixed with 2 L reservoir buffer (53-60% saturated ammonium sulfate and 50 mM TEA, pH 7.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.79 Å R-free 0.188 |
| 3ZZJ Structure of an engineered aspartate aminotransferase Deposited 2011-09-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：YES | PLP PYRIDOXAL-5'-PHOSPHATE × 2 SO4 SULFATE ION × 4 PEG DI(HYDROXYETHYL)ETHER × 4 BME BETA-MERCAPTOETHANOL × 2 GOL GLYCEROL × 10 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 2.50 Å R-free 0.262 |
| 3ZZK Structure of an engineered aspartate aminotransferase Deposited 2011-09-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：YES | PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 SO4 SULFATE ION × 8 GOL GLYCEROL × 10 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 1.78 Å R-free 0.199 |
| 4A00 Structure of an engineered aspartate aminotransferase Deposited 2011-09-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：YES | PP3 ALANYL-PYRIDOXAL-5'-PHOSPHATE × 2 PEG DI(HYDROXYETHYL)ETHER × 4 GOL GLYCEROL × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 2.34 Å R-free 0.256 |
| 4DBC Substrate Activation in Aspartate Aminotransferase Deposited 2012-01-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Mutation：K258A | SO4 SULFATE ION × 8 EDO 1,2-ETHANEDIOL × 2 3QP (E)-N-{2-hydroxy-3-methyl-6-[(phosphonooxy)methyl]benzylidene}-L-aspartic acid × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 uL of protein solution(15-20 mg/ml, 50 mM TEA, pH 7.5, 100 mM KCL, 2 mM DTT, 10 mM deaza-PLP, 50 mM L-aspartate) mixed with 2 uL reservoir buffer (53-60% saturated ammonium sulfate and 50 mM TEA), VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.50 Å R-free 0.194 |
| 4F5F Structure of Aspartate Aminotransferase Conversion to Tyrosine Aminotransferase: Chimera P1. Deposited 2012-05-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：I39V, N40D, I43V, N74T, I78L, I81L, T114S, A120G, S145A, V146I, F220I, A228G, V385I Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：I39V, N40D, I43V, N74T, I78L, I81L, T114S, A120G, S145A, V146I, F220I, A228G, V385I Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；277 K；8% PEG 4000, 0.1 M Sodium Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.25 Å R-free 0.213 |
| 4F5G Rational Design and Directed Evolution of E. coli Apartate Aminotransferase to Tyrosine Aminotransferase: Mutant P2. Deposited 2012-05-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：I39V, N40D, I43V, N74T, I78L, I81L, T114S, S145A, V146I, I197A, F220I, F222I, A228G, Y254C, S283G, I351V Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：I39V, N40D, I43V, N74T, I78L, I81L, T114S, S145A, V146I, I197A, F220I, F222I, A228G, Y254C, S283G, I351V Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；277 K；8% PEG 4000, 0.1 M Sodium Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.67 Å R-free 0.191 |
| 4F5H Intercoversion of Substrate Specificity: E. coli Aspatate Aminotransferase to Tyrosine Aminotransferase: Chimera P3. Deposited 2012-05-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：I39V, N40D, L56M, N74T, I78L, I81L, T114S, S145A, V146I, I197A, F220I, A228G, N295S, M331L Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：I39V, N40D, L56M, N74T, I78L, I81L, T114S, S145A, V146I, I197A, F220I, A228G, N295S, M331L Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；8% PEG 4000, 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.187 |
| 4F5I Substrate Specificity Conversion of E. coli Pyridoxal-5'-Phosphate Dependent Aspartate Aminotransferase to Tyrosine Aminotransferase: Chimera P4. Deposited 2012-05-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：I39V, N40D, L56M, N74T, T114S, S139T, S145A, V146I, F220I, F222I, A228G, Y254C, G259S, N295S, V385I Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：I39V, N40D, L56M, N74T, T114S, S139T, S145A, V146I, F220I, F222I, A228G, Y254C, G259S, N295S, V385I Non-standard monomer：Yes (specific site not provided by mmCIF) | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；8% PEG 4000, 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.212 |
| 4F5J Rational Design and Directed Evolution for Conversion of Substrate Specificity from E.coli Aspartate Aminotransferase to Tyrosine Aminotransferase: Mutant P5. Deposited 2012-05-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：I39V, N40D, I43V, N74T, I78L, I81L, T114S, S145A, V146I, I197A, F220I, F222I, A228G, Y254C Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：I39V, N40D, I43V, N74T, I78L, I81L, T114S, S145A, V146I, I197A, F220I, F222I, A228G, Y254C Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；277 K；8% PEG 4000, 0.1 M Sodium Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.95 Å R-free 0.204 |
| 4F5K Substrate Specificity Conversion of Aspartate Aminotransferase to Tyrosine Aminotransferase By The JANUS Algorithm: Chimera P6. Deposited 2012-05-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：I39V, N40D, I43V, L56M, N74T, I78L, I81L, T114S, S139T, S145A, V146I, I197A, F220I, F222I, A228G, Y254C, G259S, S283G, V385I Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：I39V, N40D, I43V, L56M, N74T, I78L, I81L, T114S, S139T, S145A, V146I, I197A, F220I, F222I, A228G, Y254C, G259S, S283G, V385I Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；277 K；8% PEG 4000, 0.1 M Sodium Acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.227 |
| 4F5L A Theoretical Optimized Mutant for the Conversion of Substrate Specificity and Activity of Aspartate Aminotransferase to Tyrosine Aminotransferase: Chimera P7. Deposited 2012-05-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：I39V, N40D, I43V, L56M, N74T, I78L, I81L, T114S, S139T, S145A, V146I, I197A, F220I, F222I, A228G, Y254C, N295S, V385I Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：I39V, N40D, I43V, L56M, N74T, I78L, I81L, T114S, S139T, S145A, V146I, I197A, F220I, F222I, A228G, Y254C, N295S, V385I Non-standard monomer：Yes (specific site not provided by mmCIF) | EDO 1,2-ETHANEDIOL × 13 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；277 K；8% PEG 4000, 0.1 M Sodium Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.40 Å R-free 0.181 |
| 4F5M Wild-Type E. coli Aspartate Aminotransferase: A Template For The Interconversion of Substrate Specificity and Activity To Tyrosine Aminotransferase By The JANUS Algorithm. Deposited 2012-05-13 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | EDO 1,2-ETHANEDIOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；277 K；8% PEG 4000, 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.65 Å R-free 0.210 |
| 5EAA ASPARTATE AMINOTRANSFERASE FROM E. COLI, C191S MUTATION Deposited 1998-12-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Fragment：COMPLETE SUBUNIT
|
Mutation：YES | PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.50
|
Resolution 2.40 Å |
| 5T4L PLP and GABA Trigger GabR-Mediated Transcription Regulation in Bacillus subsidies via External Aldimine Formation Deposited 2016-08-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | 77E (4R)-4-amino-6-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}hexanoic acid × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；25 mM Potassium phosphate
43% Ammonium sulfate
|
Resolution 1.53 Å R-free 0.166 |
| 5VWQ E.coli Aspartate aminotransferase-(1R,3S,4S)-3-amino-4-fluorocyclopentane-1-carboxylic acid (FCP) Deposited 2017-05-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
Chain D
1–396（396 aa）
|
Not recorded | PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；25 mM potassium phosphate and 43% saturated ammonium sulfate
|
Resolution 1.80 Å R-free 0.192 |
| 5VWQ E.coli Aspartate aminotransferase-(1R,3S,4S)-3-amino-4-fluorocyclopentane-1-carboxylic acid (FCP) Deposited 2017-05-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain G
1–396（396 aa）
Chain J
1–396（396 aa）
|
Not recorded | PMP 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；25 mM potassium phosphate and 43% saturated ammonium sulfate
|
Resolution 1.80 Å R-free 0.192 |
| 5VWR E.coli Aspartate aminotransferase-(1R,3S,4S)-3-amino-4-fluorocyclopentane-1-carboxylic acid (FCP)-alpha-ketoglutarate Deposited 2017-05-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
1–396（396 aa）
|
Not recorded | PL6 (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-glutamic acid × 2 GOL GLYCEROL × 10 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；25 mM potassium phosphate, 43% saturated ammonium sulfate
|
Resolution 1.72 Å R-free 0.190 |
| 8E9C Crystal structure of E. coli aspartate aminotransferase mutant AIFS in the ligand-free form at 100 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：V35A K37I T43F N64S Mutation：V35A K37I T43F N64S | PLP PYRIDOXAL-5'-PHOSPHATE × 2 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；Ammonium sulfate, HEPES, PEG-400, maleate
|
Resolution 2.18 Å R-free 0.224 |
| 8E9D Crystal structure of E. coli aspartate aminotransferase mutant AIFS bound to maleic acid at 100 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Not recorded | MAE MALEIC ACID × 2 PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEG 400, maleate, ammonium sulfate, HEPES
|
Resolution 1.37 Å R-free 0.160 |
| 8E9J Crystal structure of E. coli aspartate aminotransferase mutant HEX in the ligand-free form at 278 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：V35L, K37Y, T43I, N64L, T104S, N285S Mutation：V35L, K37Y, T43I, N64L, T104S, N285S | PLP PYRIDOXAL-5'-PHOSPHATE × 2 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, PEG 400, maleate
|
Resolution 2.09 Å R-free 0.198 |
| 8E9K Crystal structure of wild-type E. coli aspartate aminotransferase bound to maleate at 278 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, maleate, PEG 400
|
Resolution 1.83 Å R-free 0.183 |
| 8E9L Crystal structure of E. coli aspartate aminotransferase mutant VFIT in the ligand-free form at 278 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：K37F, T43I, N64T Mutation：K37F, T43I, N64T | PLP PYRIDOXAL-5'-PHOSPHATE × 2 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, maleate, PEG 400, ammonium sulfate
|
Resolution 2.31 Å R-free 0.222 |
| 8E9M Crystal structure of E. coli aspartate aminotransferase mutant VFIT bound to maleic acid at 278 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, maleate, PEG 400
|
Resolution 1.76 Å R-free 0.175 |
| 8E9N Crystal structure of E. coli aspartate aminotransferase mutant VFIY in the ligand-free form at 278 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：K37F, T43I, N64Y Mutation：K37F, T43I, N64Y | SO4 SULFATE ION × 2 PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, maleate, PEG 400
|
Resolution 1.88 Å R-free 0.178 |
| 8E9O Crystal structure of E. coli aspartate aminotransferase mutant VFIY bound to maleic acid at 278 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, PEG 400, maleate
|
Resolution 1.96 Å R-free 0.189 |
| 8E9P Crystal structure of wild-type E. coli aspartate aminotransferase in the ligand-free form at 278 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, PEG 400, maleate
|
Resolution 2.08 Å R-free 0.205 |
| 8E9Q Crystal structure of E. coli aspartate aminotransferase mutant HEX bound to maleic acid at 278 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, PEG 400, maleate
|
Resolution 1.80 Å R-free 0.172 |
| 8E9R Crystal structure of E. coli aspartate aminotransferase mutant VFCS in the ligand-free form at 278 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：K37F, T43C, N64S Mutation：K37F, T43C, N64S | SO4 SULFATE ION × 2 PLP PYRIDOXAL-5'-PHOSPHATE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, maleate, ammonium sulfate, PEG 400
|
Resolution 1.90 Å R-free 0.191 |
| 8E9S Crystal structure of E. coli aspartate aminotransferase mutant VFCS bound to maleic acid at 278 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 MAE MALEIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, PEG 400, maleate
|
Resolution 2.00 Å R-free 0.185 |
| 8E9T Crystal structure of wild-type E. coli aspartate aminotransferase in the ligand-free form at 303 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, PEG 400, maleate
|
Resolution 2.13 Å R-free 0.200 |
| 8E9U Crystal structure of E. coli aspartate aminotransferase mutant HEX in the ligand-free form at 303 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Mutation：V35L, K37Y, T43I, N64L, T104S, N285S Mutation：V35L, K37Y, T43I, N64L, T104S, N285S | PLP PYRIDOXAL-5'-PHOSPHATE × 2 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, PEG 400, maleate
|
Resolution 1.94 Å R-free 0.178 |
| 8E9V Crystal structure of E. coli aspartate aminotransferase mutant VFIT in the ligand-free form at 303 K Deposited 2022-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
2–396（395 aa）
Chain B
2–396（395 aa）
|
Not recorded | PLP PYRIDOXAL-5'-PHOSPHATE × 2 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；HEPES, ammonium sulfate, PEG 400, maleate
|
Resolution 2.01 Å R-free 0.196 |
103 other PDB entries and 104 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | AAT_ECOLI |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–388； UniProt 1–396 |
