1tug

Aspartate Transcarbamoylase Catalytic Chain Mutant E50A Complex with Phosphonoacetamide, Malonate, and Cytidine-5-Prime-Triphosphate (CTP)

Method: X-RAY DIFFRACTION D_max: 104.0 Å Quality: GOOD

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Aspartate carbamoyltransferase catalytic chain

Escherichia coli

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 12 PDB 声明：dodecameric（12）与蛋白拷贝数一致	链 A； UniProt 1–310 链 C； UniProt 1–310	突变：E50A	Aspartate carbamoyltransferase regulatory chain × 6 （P0A7F3） MLI MALONATE ION × 6 PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件：pH 6；295 K；100 mM sodium citrate, 1 mM 2-mercaptoethanol, 0.2 mM EDTA and 1.0 mM CTP pH 6.0 then soaked for 24 hours in 100 mM malonate, 2 mM CTP, 3 mM sodium azide, 2 mM 2-mercaptoethanol, and 15% PEG 8000 pH 7.0. Before mounting, 50 mM PAM was added and the crystal allowed to soak until data collected., MICRODIALYSIS, temperature 295K, pH 6.00	分辨率 2.10 Å R-free 0.272

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 1TUG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1ACM ARGININE 54 IN THE ACTIVE SITE OF ESCHERICHIA COLI ASPARTATE TRANSCARBAMOYLASE IS CRITICAL FOR CATALYSIS: A SITE-SPECIFIC MUTAGENESIS, NMR AND X-RAY CRYSTALLOGRAPHY STUDY 提交 1992-07-08	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å
1AT1 CRYSTAL STRUCTURES OF PHOSPHONOACETAMIDE LIGATED T AND PHOSPHONOACETAMIDE AND MALONATE LIGATED R STATES OF ASPARTATE CARBAMOYLTRANSFERASE AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL P*H 提交 1989-09-22	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	MLI MALONATE ION × 6 PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å
1D09 ASPARTATE TRANSCARBAMOYLASE COMPLEXED WITH N-PHOSPHONACETYL-L-ASPARTATE (PALA) 提交 1999-09-09	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 LIQUID DIFFUSION；pH 5.9；ENZYME: 12 MG/ML; BUFFER: 50 MM MALEIC ACID, 3 MM SOLDIUM AZIDE 1 MM N- PHOSPHONACETYL-L-ASPARTATE, pH 5.9, LIQUID DIFFUSION	分辨率 2.10 Å R-free 0.234
1EKX THE ISOLATED, UNREGULATED CATALYTIC TRIMER OF ASPARTATE TRANSCARBAMOYLASE COMPLEXED WITH BISUBSTRATE ANALOG PALA (N-(PHOSPHONACETYL)-L-ASPARTATE) 提交 2000-03-09	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 1–311（311 aa）片段：CATALYTIC SUBUNIT 链 B 1–311（311 aa）片段：CATALYTIC SUBUNIT 链 C 1–311（311 aa）片段：CATALYTIC SUBUNIT	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 3 CA CALCIUM ION × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；Calcium Acetate, PEG 8000, tris-HCL, 2-mercaptoethanol, PALA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 1.95 Å R-free 0.214
1EZZ CRYSTAL STRUCTURE OF E. COLI ASPARTATE TRANSCARBAMOYLASE P268A MUTANT IN THE T-STATE 提交 2000-05-12	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	突变：P268A 突变：P268A	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；ENZYME: 24 mg/ml; 1:1 enzyme to-buffer ratio, BUFFER: 20 mm HEPES, 14% (w/v) PEG 1450, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	分辨率 2.70 Å R-free 0.242
1F1B CRYSTAL STRUCTURE OF E. COLI ASPARTATE TRANSCARBAMOYLASE P268A MUTANT IN THE R-STATE IN THE PRESENCE OF N-PHOSPHONACETYL-L-ASPARTATE 提交 2000-05-18	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	突变：P268A 突变：P268A	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.75；293 K；ENZYME: 7.5 mg/ml in 50 uL microdialysis button. BUFFER: 20 mm maleic acid, 3 mM sodium azide, 1 mM N-phosphonacetyl-L-aspartate, pH 5.75, MICRODIALYSIS, temperature 293K	分辨率 2.30 Å R-free 0.232
1I5O CRYSTAL STRUCTURE OF MUTANT R105A OF E. COLI ASPARTATE TRANSCARBAMOYLASE 提交 2001-02-28	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	突变：R105A 突变：R105A	ZN ZINC ION × 6 PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 3	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；293 K；Tris buffer, maleic acid, pH 5.7, MICRODIALYSIS, temperature 293K	分辨率 2.80 Å R-free 0.212
1NBE ASPARTATE TRANSCARBAMOYLASE REGULATORY CHAIN MUTANT (T82A) 提交 1998-04-25	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	突变：T82A 突变：T82A	MLT D-MALATE × 12 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 5.85；7.5 MG/ML PROTEIN DIALYZED AGAINST 100MM MALIC ACID AT PH 5.85,	分辨率 2.60 Å R-free 0.237
1Q95 Aspartate Transcarbamylase (ATCase) of Escherichia coli: A New Crystalline R State Bound to PALA, or to Product Analogues Phosphate and Citrate 提交 2003-08-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 B 1–310（310 aa）链 C 1–310（310 aa）链 D 1–310（310 aa）链 E 1–310（310 aa）链 F 1–310（310 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；294 K；PEG-mmes 2000, lithium sulfate, sodium azide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K	分辨率 2.46 Å R-free 0.270
1R0B Aspartate Transcarbamylase (ATCase) of Escherichia coli: A New Crystalline R State Bound to PALA, or to Product Analogues Phosphate and Citrate 提交 2003-09-19	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 B 1–310（310 aa）链 C 1–310（310 aa）链 D 1–310（310 aa）链 E 1–310（310 aa）链 F 1–310（310 aa）	未记录	FLC CITRATE ANION × 6 PO4 PHOSPHATE ION × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.8；294 K；sodium citrate, sodium phosphate, N-ethylmorpholine, pH 5.8, MICRODIALYSIS, temperature 294K	分辨率 2.90 Å R-free 0.300
1R0C Products in the T State of Aspartate Transcarbamylase: Crystal Structure of the Phosphate and N-carbamyl-L-aspartate Ligated Enzyme 提交 2003-09-19	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 G 1–310（310 aa）	未记录	PO4 PHOSPHATE ION × 6 NCD N-CARBAMOYL-L-ASPARTATE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；294 K；PEG 4000, Hepes-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K	分辨率 2.37 Å R-free 0.274
1RAA CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAB CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAC CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAD CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAE CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAF CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAG CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAH CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAI CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1SKU E. coli Aspartate Transcarbamylase 240's Loop Mutant (K244N) 提交 2004-03-05	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	突变：K244N 突变：K244N	MLI MALONATE ION × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；Prior to crystallization the enzyme was dialyzed into 40 mM KH2PO4, 2.0 mM 2-mercaptoethanol, 0.2 mM EDTA, pH 7.0 for 24 hours. Single crystals of the K244N mutant were obtained by mixing a 20 mg/ml filtered solution (0.22 m) of the K244N enzyme with a solution of 17% (w/v) PEG 1450, 50 mM malonate, 0.2 mM EDTA, 1 mM sodium azide and 20 mM Bis-Tris buffer pH 7.0 in a 1:1 ratio (v/v), VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.60 Å R-free 0.231
1TTH Aspartate Transcarbamoylase Catalytic Chain Mutant Glu50Ala Complexed with N-(Phosphonacetyl-L-Aspartate) (PALA) 提交 2004-06-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	突变：E50A 突变：E50A	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；295 K；50 mM maleic-acid-N-ethyl-morpholine buffer (pH 5.84) containing 3 mM sodium azide and 1 mM PALA, pH 5.90, MICRODIALYSIS, temperature 295K	分辨率 2.80 Å R-free 0.264
1TU0 Aspartate Transcarbamoylase Catalytic Chain Mutant E50A Complex with Phosphonoacetamide 提交 2004-06-23	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	突变：E50A 突变：E50A	PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6；295 K；The Glu50Ala mutant was crystallized in 100 mM sodium citrate, 1 mM 2-mercaptoethanol, and 15% PEG 8000 pH 7.0. Before mounting, 50 mM PAM was added and the crystal soaked, pH 6.0, MICRODIALYSIS, temperature 295K, pH 6.00	分辨率 2.55 Å R-free 0.279
1XJW The Structure of E. coli Aspartate Transcarbamoylase Q137A Mutant in The R-State 提交 2004-09-25	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	突变：Q137A 突变：Q137A	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.9；295 K；12 mg/ml Buffer 50 mM Maleic Acid, 3 mM Sodium Azide 1 mM N-Phosphonacetyl-L-Aspartate, pH 5.9, MICRODIALYSIS, temperature 295K	分辨率 2.71 Å R-free 0.223
2A0F Structure of D236A mutant E. coli Aspartate Transcarbamoylase in presence of Phosphonoacetamide at 2.90 A resolution 提交 2005-06-16	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	突变：D236A 突变：D236A	PCT PHOSPHONOACETAMIDE × 3 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；maleic acid, sodium azide, phosphonoacetamide, pH 5.7, MICRODIALYSIS, temperature 298K	分辨率 2.90 Å R-free 0.279
2AIR T-state Active Site of Aspartate Transcarbamylase:Crystal Structure of the Carbamyl Phosphate and L-alanosine Ligated Enzyme 提交 2005-07-30	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 G 1–310（310 aa）	未记录	CP PHOSPHORIC ACID MONO(FORMAMIDE)ESTER × 6 AL0 3-[HYDROXY(NITROSO)AMINO]-L-ALANINE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；294 K；PEG-4000, iso-propranol, sodium azide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K	分辨率 2.00 Å R-free 0.273
2AT1 CRYSTAL STRUCTURES OF PHOSPHONOACETAMIDE LIGATED T AND PHOSPHONOACETAMIDE AND MALONATE LIGATED R STATES OF ASPARTATE CARBAMOYLTRANSFERASE AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL PH 提交 1989-09-22	突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å
2ATC CRYSTAL AND MOLECULAR STRUCTURES OF NATIVE AND CTP-LIGANDED ASPARTATE CARBAMOYLTRANSFERASE FROM ESCHERICHIA COLI 提交 1982-03-24	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 3.00 Å
2FZC The Structure of Wild-Type E. Coli Aspartate Transcarbamoylase in Complex with Novel T State Inhibitors at 2.10 Resolution 提交 2006-02-09	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	EOP {ETHANE-1,2-DIYLBIS[IMINO(2-OXOETHANE-2,1-DIYL)]}BIS(PHOSPHONIC ACID) × 6 ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 5.7；298 K；ATCase holoenzyme was crystallized by microdialysis, using 50 L wells. The enzyme solution, at ~18 mg/mL, was dialyzed against a solution of 40 mM citric acid, 3 mM sodium azide, 1 mM 2-mercaptoethanol, 1 mM cytidine 5 -triphosphate, 0.2 mM EDTA (pH 5.7) , VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.10 Å R-free 0.250
2FZG The Structure of Wild-Type E. Coli Aspartate Transcarbamoylase in Complex with Novel T State Inhibitors at 2.25 Resolution 提交 2006-02-09	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	EOB {1,3-PHENYLENEBIS[IMINO(2-OXOETHANE-2,1-DIYL)]}BIS(PHOSPHONIC ACID) × 6 ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；ATCase holoenzyme was crystallized by microdialysis, using 50 L wells. The enzyme solution, at ~18 mg/mL, was dialyzed against a solution of 40 mM citric acid, 3 mM sodium azide, 1 mM 2-mercaptoethanol, 1 mM cytidine 5 -triphosphate, 0.2 mM EDTA (pH 5.7), MICRODIALYSIS, temperature 298K	分辨率 2.25 Å R-free 0.237
2FZK The Structure of Wild-Type E. Coli Aspartate Transcarbamoylase in Complex with Novel T State Inhibitors at 2.50 Resolution 提交 2006-02-09	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	EOZ 3,5-BIS[(PHOSPHONOACETYL)AMINO]BENZOIC ACID × 6 ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；ATCase holoenzyme was crystallized by microdialysis, using 50 L wells. The enzyme solution, at ~18 mg/mL, was dialyzed against a solution of 40 mM citric acid, 3 mM sodium azide, 1 mM 2-mercaptoethanol, 1 mM cytidine 5 -triphosphate, 0.2 mM EDTA (pH 5.7), MICRODIALYSIS, temperature 298K	分辨率 2.50 Å R-free 0.239
2H3E Structure of wild-type E. coli Aspartate Transcarbamoylase in the presence of N-phosphonacetyl-L-isoasparagine at 2.3A resolution 提交 2006-05-22	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	6PR (S)-4-AMINO-4-OXO-3-(2-PHOSPHONOACETAMIDO)BUTANOIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；293 K；50 mM Maleic acid, 3 mM sodium azide, 1 mM N-phosphonacetyl-L-isoasparagine, pH 5.7, MICRODIALYSIS, temperature 293K	分辨率 2.30 Å R-free 0.250
2HSE Structure of D236A E. coli Aspartate Transcarbamoylase in the presence of phosphonoacetamide and l-Aspartate at 2.60 A resolution 提交 2006-07-21	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	PO4 PHOSPHATE ION × 3 PCT PHOSPHONOACETAMIDE × 6 ASP ASPARTIC ACID × 9 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.8；293 K；50mM maleic acid, 3mM sodium azide, 10mM phosphonoacetamide, 20mM L-aspartate, pH 5.8, MICRODIALYSIS, temperature 293K	分辨率 2.60 Å R-free 0.254
2IPO E. coli Aspartate Transcarbamoylase complexed with N-phosphonacetyl-L-asparagine 提交 2006-10-12	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	1IP N~2~-(PHOSPHONOACETYL)-L-ASPARAGINE × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 6 ZN ZINC ION × 6 MAE MALEIC ACID × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；295 K；50 mM Maleic acid, 1 mM N-phosphonacetyl-L-asparagine, 3 mM Sodium Azide, pH 5.7, MICRODIALYSIS, temperature 295K	分辨率 2.60 Å R-free 0.253
2QG9 Structure of a regulatory subunit mutant D19A of ATCase from E. coli 提交 2007-06-28	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；0.1M citrate buffer, pH 5.7, MICRODIALYSIS, temperature 298.0K	分辨率 2.70 Å R-free 0.244
2QGF Structure of regulatory chain mutant H20A of asparate transcarbamoylase from E. coli 提交 2007-06-28	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；0.1M citric acid, pH 5.7, MICRODIALYSIS, temperature 298.0K	分辨率 2.20 Å R-free 0.223
3AT1 CRYSTAL STRUCTURES OF PHOSPHONOACETAMIDE LIGATED T AND PHOSPHONOACETAMIDE AND MALONATE LIGATED R STATES OF ASPARTATE CARBAMOYLTRANSFERASE AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL PH 提交 1989-09-22	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å
3CSU CATALYTIC TRIMER OF ESCHERICHIA COLI ASPARTATE TRANSCARBAMOYLASE 提交 1999-04-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 1–310（310 aa）片段：CATALYTIC SUBUNIT 链 B 1–310（310 aa）片段：CATALYTIC SUBUNIT 链 C 1–310（310 aa）片段：CATALYTIC SUBUNIT	未记录	CA CALCIUM ION × 2	X-RAY DIFFRACTION X-ray结晶条件 pH 7.8；pH 7.8	分辨率 1.88 Å R-free 0.291
3D7S Crystal structure of Wild-Type E. Coli Asparate Transcarbamoylase at pH 8.5 at 2.80 A Resolution 提交 2008-05-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；50 mM Tris 8K PEG, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.80 Å R-free 0.235
3MPU Crystal structure of the C47A/A241C disulfide-linked E. coli Aspartate Transcarbamoylase holoenzyme 提交 2010-04-27	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	突变：C47A, A241C 突变：C47A, A241C	PO4 PHOSPHATE ION × 12 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.9；293 K；Protein at 10 mg/ml was dialyzed against solution containing 100 mM KH2PO4, 3mM NaN3, pH 5.9, MICRODIALYSIS, temperature 293K	分辨率 2.85 Å R-free 0.240
3MPU Crystal structure of the C47A/A241C disulfide-linked E. coli Aspartate Transcarbamoylase holoenzyme 提交 2010-04-27	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 E 2–311（310 aa）	突变：C47A, A241C	PO4 PHOSPHATE ION × 12 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.9；293 K；Protein at 10 mg/ml was dialyzed against solution containing 100 mM KH2PO4, 3mM NaN3, pH 5.9, MICRODIALYSIS, temperature 293K	分辨率 2.85 Å R-free 0.240
3NPM Crystal Structure of the C47A/A241C disulfide-linked C6 Aspartate Transcarbamoylase enzyme 提交 2010-06-28	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 6 PDB 声明：hexameric	链 A 2–311（310 aa）	突变：A48C; C242A	PO4 PHOSPHATE ION × 12	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；10 mg/mL of C47A/A241C c6 in 100 mM tris-acetate pH 8.3, in a 1:1 ratio (v/v), with reservoir. Crystallization buffer consisted of 40% PEG 1000, 100 mM NH4H2PO4, and 100 mM HEPES, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.10 Å R-free 0.205
4AT1 STRUCTURAL CONSEQUENCES OF EFFECTOR BINDING TO THE T STATE OF ASPARTATE CARBAMOYLTRANSFERASE. CRYSTAL STRUCTURES OF THE UNLIGATED AND ATP-, AND CTP-COMPLEXED ENZYMES AT 2.6-ANGSTROMS RESOLUTION 提交 1990-04-26	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.60 Å
4E2F Crystal Structure of E. coli Aspartate Transcarbamoylase K164E/E239K Mutant in an intermediate state 提交 2012-03-08	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）链 E 2–311（310 aa）链 G 2–311（310 aa）链 I 2–311（310 aa）链 K 2–311（310 aa）	突变：K164E, E239K 突变：K164E, E239K 突变：K164E, E239K 突变：K164E, E239K 突变：K164E, E239K 突变：K164E, E239K	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8.75；293 K；16% (w/v) PEG 4000, 0.04 M Na2MoO4, 0.04 M N-cyclohexyl-3-aminopropanesulfonic acid, 30 mM Tris-acetate, pH 8.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.80 Å R-free 0.274
4F04 A Second Allosteric site in E. coli Aspartate Transcarbamoylase: R-state ATCase with UTP bound 提交 2012-05-03	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6 UTP URIDINE 5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.95；298 K；50 mM maleic acid, 3 mM sodium azide, 1 mM PALA, adjusted to pH=5.95 with N-ethylmorpholine, MICRODIALYSIS, temperature 298K	分辨率 2.30 Å R-free 0.207
4FYV Aspartate Transcarbamoylase Complexed with dCTP 提交 2012-07-05	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	PO4 PHOSPHATE ION × 3 ZN ZINC ION × 6 DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；40 mM sodium citrate, 1 mM 2-mercaptoethanol, 0.2 mM EDTA, 1.0 mM dCTP, pH 5.7, MICRODIALYSIS, temperature 298K	分辨率 2.10 Å R-free 0.215
4FYW E. coli Aspartate Transcarbamoylase complexed with CTP 提交 2012-07-05	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；40 mM sodium citrate, 1 mM 2-mercaptoethanol, 0.2 mM EDTA, 1.0 mM CTP, pH 5.7, MICRODIALYSIS, temperature 298K	分辨率 2.10 Å R-free 0.229
4FYX E. coli Aspartate Transcarbamoylase complexed with dCTP, UTP, and Mg2+ 提交 2012-07-05	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 UTP URIDINE 5'-TRIPHOSPHATE × 6 DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；40 mM sodium citrate, 1 mM 2-mercaptoethanol, 0.2 mM EDTA, 1.0 mM dCTP, pH 5.7 (crystals soaked in 5 mM UTP and 5 mM Mg2+), MICRODIALYSIS, temperature 298K	分辨率 2.09 Å R-free 0.212
4FYY E. coli Aspartate Transcarbamoylase Complexed with CTP, UTP, and Mg2+ 提交 2012-07-05	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）	未记录	ZN ZINC ION × 6 UTP URIDINE 5'-TRIPHOSPHATE × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；40 mM sodium citrate, 1 mM 2-mercaptoethanol, 0.2 mM EDTA, 1.0 mM CTP, pH 5.7 (crystals soaked in 5 mM UTP and 5 mM Mg2+), MICRODIALYSIS, temperature 298K	分辨率 1.94 Å R-free 0.236
5AT1 STRUCTURAL CONSEQUENCES OF EFFECTOR BINDING TO THE T STATE OF ASPARTATE CARBAMOYLTRANSFERASE. CRYSTAL STRUCTURES OF THE UNLIGATED AND ATP-, AND CTP-COMPLEXED ENZYMES AT 2.6-ANGSTROMS RESOLUTION 提交 1990-04-26	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.60 Å
6AT1 STRUCTURAL CONSEQUENCES OF EFFECTOR BINDING TO THE T STATE OF ASPARTATE CARBAMOYLTRANSFERASE. CRYSTAL STRUCTURES OF THE UNLIGATED AND ATP-, AND CTP-COMPLEXED ENZYMES AT 2.6-ANGSTROMS RESOLUTION 提交 1990-04-26	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.60 Å
6KJ7 E. coli ATCase catalytic subunit mutant - G166P 提交 2019-07-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 2–311（310 aa）	突变：G166P	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；0.2M NH4Ac, 0.1 M Tris pH 8.5, 20% PEG3350, and 10% glycerol	分辨率 2.84 Å R-free 0.260
6KJ8 E. coli ATCase holoenzyme mutant - G166P (catalytic chain) 提交 2019-07-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）链 E 2–311（310 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；0.1M HEPES pH 7.0, 30% Jeffamine M-600 pH 7.0, and 10% glycerol	分辨率 3.01 Å R-free 0.247
6KJ9 E. coli ATCase catalytic subunit mutant - G128/130A 提交 2019-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 2–311（310 aa）链 B 2–311（310 aa）链 E 2–311（310 aa）	突变：G128A, G130A 突变：G128A, G130A 突变：G128A, G130A	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；0.2M NH4Ac, 0.1M Tris pH 8.5, 20% PEG3350, and 10% glycerol	分辨率 2.50 Å R-free 0.228
6KJ9 E. coli ATCase catalytic subunit mutant - G128/130A 提交 2019-07-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白同源多聚体同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 C 2–311（310 aa）链 D 2–311（310 aa）链 F 2–311（310 aa）	突变：G128A, G130A 突变：G128A, G130A 突变：G128A, G130A	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；0.2M NH4Ac, 0.1M Tris pH 8.5, 20% PEG3350, and 10% glycerol	分辨率 2.50 Å R-free 0.228
6KJA E. coli ATCase holoenzyme mutant - G128/130A (catalytic chain) 提交 2019-07-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）链 C 2–311（310 aa）链 E 2–311（310 aa）	突变：G128A, G130A 突变：G128A, G130A 突变：G128A, G130A	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M HEPES pH 7.0, 30% Jeffamine M-600 pH 7.0, and 10% glycerol	分辨率 3.06 Å R-free 0.283
6KJB wild-type apo-form E. coli ATCase holoenzyme with an unusual open conformation of R167 提交 2019-07-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 2–311（310 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；0.1M MES pH 6.0 10% Glycerol and 10% PEG 8000	分辨率 2.06 Å R-free 0.223
7AT1 CRYSTAL STRUCTURES OF ASPARTATE CARBAMOYLTRANSFERASE LIGATED WITH PHOSPHONOACETAMIDE, MALONATE, AND CTP OR ATP AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL P*H 提交 1989-09-22	突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 3	X-RAY DIFFRACTION X-ray结晶条件 THE CRYSTALS WERE GROWN IN A SOLUTION OF PHOSPHONACETAMIDE (PAM) AND MALONATE (MAL) AT PH 5.8. THEY WERE THEN SOAKED IN A SOLUTION CONTAINING PAM, MAL, AND ATP AT PH 7.0.	分辨率 2.80 Å
8AT1 CRYSTAL STRUCTURES OF ASPARTATE CARBAMOYLTRANSFERASE LIGATED WITH PHOSPHONOACETAMIDE, MALONATE, AND CTP OR ATP AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL P*H 提交 1989-09-22	突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 THE CRYSTALS WERE GROWN IN A SOLUTION OF PHOSPHONACETAMIDE (PAM) AND MALONATE (MAL) AT PH 5.8. THEY WERE THEN SOAKED IN A SOLUTION CONTAINING PAM, MAL, AND CTP AT PH 7.0.	分辨率 2.80 Å
8ATC COMPLEX OF N-PHOSPHONACETYL-L-ASPARTATE WITH ASPARTATE CARBAMOYLTRANSFERASE. X-RAY REFINEMENT, ANALYSIS OF CONFORMATIONAL CHANGES AND CATALYTIC AND ALLOSTERIC MECHANISMS 提交 1989-08-25	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）链 C 1–310（310 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
9ATC ATCASE Y165F MUTANT 提交 1998-06-26	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 A 1–310（310 aa）	突变：CHAIN A, Y165F	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6.3；pH 6.3	分辨率 2.40 Å R-free 0.344

共 59 个其他 PDB 条目、61 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	PYRB_ECOLI
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–310； UniProt 1–310 作者链 C； PDB构建体 1–310； UniProt 1–310

Aspartate carbamoyltransferase regulatory chain

Escherichia coli

UniProt P0A7F3

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白异源复合物异源复合物蛋白 × 12 PDB 声明：dodecameric（12）与蛋白拷贝数一致	链 B； UniProt 1–152 链 D； UniProt 1–152	未记录	Aspartate carbamoyltransferase catalytic chain × 6 （P0A786） MLI MALONATE ION × 6 PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件：pH 6；295 K；100 mM sodium citrate, 1 mM 2-mercaptoethanol, 0.2 mM EDTA and 1.0 mM CTP pH 6.0 then soaked for 24 hours in 100 mM malonate, 2 mM CTP, 3 mM sodium azide, 2 mM 2-mercaptoethanol, and 15% PEG 8000 pH 7.0. Before mounting, 50 mM PAM was added and the crystal allowed to soak until data collected., MICRODIALYSIS, temperature 295K, pH 6.00	分辨率 2.10 Å R-free 0.272

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 1TUG	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1ACM ARGININE 54 IN THE ACTIVE SITE OF ESCHERICHIA COLI ASPARTATE TRANSCARBAMOYLASE IS CRITICAL FOR CATALYSIS: A SITE-SPECIFIC MUTAGENESIS, NMR AND X-RAY CRYSTALLOGRAPHY STUDY 提交 1992-07-08	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å
1AT1 CRYSTAL STRUCTURES OF PHOSPHONOACETAMIDE LIGATED T AND PHOSPHONOACETAMIDE AND MALONATE LIGATED R STATES OF ASPARTATE CARBAMOYLTRANSFERASE AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL P*H 提交 1989-09-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	MLI MALONATE ION × 6 PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å
1D09 ASPARTATE TRANSCARBAMOYLASE COMPLEXED WITH N-PHOSPHONACETYL-L-ASPARTATE (PALA) 提交 1999-09-09	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 LIQUID DIFFUSION；pH 5.9；ENZYME: 12 MG/ML; BUFFER: 50 MM MALEIC ACID, 3 MM SOLDIUM AZIDE 1 MM N- PHOSPHONACETYL-L-ASPARTATE, pH 5.9, LIQUID DIFFUSION	分辨率 2.10 Å R-free 0.234
1EZZ CRYSTAL STRUCTURE OF E. COLI ASPARTATE TRANSCARBAMOYLASE P268A MUTANT IN THE T-STATE 提交 2000-05-12	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；ENZYME: 24 mg/ml; 1:1 enzyme to-buffer ratio, BUFFER: 20 mm HEPES, 14% (w/v) PEG 1450, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K	分辨率 2.70 Å R-free 0.242
1F1B CRYSTAL STRUCTURE OF E. COLI ASPARTATE TRANSCARBAMOYLASE P268A MUTANT IN THE R-STATE IN THE PRESENCE OF N-PHOSPHONACETYL-L-ASPARTATE 提交 2000-05-18	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.75；293 K；ENZYME: 7.5 mg/ml in 50 uL microdialysis button. BUFFER: 20 mm maleic acid, 3 mM sodium azide, 1 mM N-phosphonacetyl-L-aspartate, pH 5.75, MICRODIALYSIS, temperature 293K	分辨率 2.30 Å R-free 0.232
1I5O CRYSTAL STRUCTURE OF MUTANT R105A OF E. COLI ASPARTATE TRANSCARBAMOYLASE 提交 2001-02-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 3	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；293 K；Tris buffer, maleic acid, pH 5.7, MICRODIALYSIS, temperature 293K	分辨率 2.80 Å R-free 0.212
1NBE ASPARTATE TRANSCARBAMOYLASE REGULATORY CHAIN MUTANT (T82A) 提交 1998-04-25	突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	突变：T82A 突变：T82A	MLT D-MALATE × 12 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 5.85；7.5 MG/ML PROTEIN DIALYZED AGAINST 100MM MALIC ACID AT PH 5.85,	分辨率 2.60 Å R-free 0.237
1Q95 Aspartate Transcarbamylase (ATCase) of Escherichia coli: A New Crystalline R State Bound to PALA, or to Product Analogues Phosphate and Citrate 提交 2003-08-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 G 1–152（152 aa）链 H 1–152（152 aa）链 I 1–152（152 aa）链 J 1–152（152 aa）链 K 1–152（152 aa）链 L 1–152（152 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；294 K；PEG-mmes 2000, lithium sulfate, sodium azide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K	分辨率 2.46 Å R-free 0.270
1R0B Aspartate Transcarbamylase (ATCase) of Escherichia coli: A New Crystalline R State Bound to PALA, or to Product Analogues Phosphate and Citrate 提交 2003-09-19	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 G 1–152（152 aa）链 H 1–152（152 aa）链 I 1–152（152 aa）链 J 1–152（152 aa）链 K 1–152（152 aa）链 L 1–152（152 aa）	未记录	FLC CITRATE ANION × 6 PO4 PHOSPHATE ION × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.8；294 K；sodium citrate, sodium phosphate, N-ethylmorpholine, pH 5.8, MICRODIALYSIS, temperature 294K	分辨率 2.90 Å R-free 0.300
1R0C Products in the T State of Aspartate Transcarbamylase: Crystal Structure of the Phosphate and N-carbamyl-L-aspartate Ligated Enzyme 提交 2003-09-19	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 H 1–152（152 aa）	未记录	PO4 PHOSPHATE ION × 6 NCD N-CARBAMOYL-L-ASPARTATE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；294 K；PEG 4000, Hepes-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K	分辨率 2.37 Å R-free 0.274
1RAA CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAB CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAC CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAD CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAE CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAF CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAG CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAH CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1RAI CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY 提交 1992-08-14	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
1SKU E. coli Aspartate Transcarbamylase 240's Loop Mutant (K244N) 提交 2004-03-05	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	MLI MALONATE ION × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；Prior to crystallization the enzyme was dialyzed into 40 mM KH2PO4, 2.0 mM 2-mercaptoethanol, 0.2 mM EDTA, pH 7.0 for 24 hours. Single crystals of the K244N mutant were obtained by mixing a 20 mg/ml filtered solution (0.22 m) of the K244N enzyme with a solution of 17% (w/v) PEG 1450, 50 mM malonate, 0.2 mM EDTA, 1 mM sodium azide and 20 mM Bis-Tris buffer pH 7.0 in a 1:1 ratio (v/v), VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.60 Å R-free 0.231
1TTH Aspartate Transcarbamoylase Catalytic Chain Mutant Glu50Ala Complexed with N-(Phosphonacetyl-L-Aspartate) (PALA) 提交 2004-06-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 5.9；295 K；50 mM maleic-acid-N-ethyl-morpholine buffer (pH 5.84) containing 3 mM sodium azide and 1 mM PALA, pH 5.90, MICRODIALYSIS, temperature 295K	分辨率 2.80 Å R-free 0.264
1TU0 Aspartate Transcarbamoylase Catalytic Chain Mutant E50A Complex with Phosphonoacetamide 提交 2004-06-23	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6；295 K；The Glu50Ala mutant was crystallized in 100 mM sodium citrate, 1 mM 2-mercaptoethanol, and 15% PEG 8000 pH 7.0. Before mounting, 50 mM PAM was added and the crystal soaked, pH 6.0, MICRODIALYSIS, temperature 295K, pH 6.00	分辨率 2.55 Å R-free 0.279
1XJW The Structure of E. coli Aspartate Transcarbamoylase Q137A Mutant in The R-State 提交 2004-09-25	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.9；295 K；12 mg/ml Buffer 50 mM Maleic Acid, 3 mM Sodium Azide 1 mM N-Phosphonacetyl-L-Aspartate, pH 5.9, MICRODIALYSIS, temperature 295K	分辨率 2.71 Å R-free 0.223
2A0F Structure of D236A mutant E. coli Aspartate Transcarbamoylase in presence of Phosphonoacetamide at 2.90 A resolution 提交 2005-06-16	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PCT PHOSPHONOACETAMIDE × 3 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；maleic acid, sodium azide, phosphonoacetamide, pH 5.7, MICRODIALYSIS, temperature 298K	分辨率 2.90 Å R-free 0.279
2AIR T-state Active Site of Aspartate Transcarbamylase:Crystal Structure of the Carbamyl Phosphate and L-alanosine Ligated Enzyme 提交 2005-07-30	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 0–152（153 aa）链 H 0–152（153 aa）	未记录	CP PHOSPHORIC ACID MONO(FORMAMIDE)ESTER × 6 AL0 3-[HYDROXY(NITROSO)AMINO]-L-ALANINE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；294 K；PEG-4000, iso-propranol, sodium azide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K	分辨率 2.00 Å R-free 0.273
2AT1 CRYSTAL STRUCTURES OF PHOSPHONOACETAMIDE LIGATED T AND PHOSPHONOACETAMIDE AND MALONATE LIGATED R STATES OF ASPARTATE CARBAMOYLTRANSFERASE AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL PH 提交 1989-09-22	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å
2ATC CRYSTAL AND MOLECULAR STRUCTURES OF NATIVE AND CTP-LIGANDED ASPARTATE CARBAMOYLTRANSFERASE FROM ESCHERICHIA COLI 提交 1982-03-24	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 3.00 Å
2FZC The Structure of Wild-Type E. Coli Aspartate Transcarbamoylase in Complex with Novel T State Inhibitors at 2.10 Resolution 提交 2006-02-09	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	EOP {ETHANE-1,2-DIYLBIS[IMINO(2-OXOETHANE-2,1-DIYL)]}BIS(PHOSPHONIC ACID) × 6 ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 5.7；298 K；ATCase holoenzyme was crystallized by microdialysis, using 50 L wells. The enzyme solution, at ~18 mg/mL, was dialyzed against a solution of 40 mM citric acid, 3 mM sodium azide, 1 mM 2-mercaptoethanol, 1 mM cytidine 5 -triphosphate, 0.2 mM EDTA (pH 5.7) , VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.10 Å R-free 0.250
2FZG The Structure of Wild-Type E. Coli Aspartate Transcarbamoylase in Complex with Novel T State Inhibitors at 2.25 Resolution 提交 2006-02-09	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	EOB {1,3-PHENYLENEBIS[IMINO(2-OXOETHANE-2,1-DIYL)]}BIS(PHOSPHONIC ACID) × 6 ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；ATCase holoenzyme was crystallized by microdialysis, using 50 L wells. The enzyme solution, at ~18 mg/mL, was dialyzed against a solution of 40 mM citric acid, 3 mM sodium azide, 1 mM 2-mercaptoethanol, 1 mM cytidine 5 -triphosphate, 0.2 mM EDTA (pH 5.7), MICRODIALYSIS, temperature 298K	分辨率 2.25 Å R-free 0.237
2FZK The Structure of Wild-Type E. Coli Aspartate Transcarbamoylase in Complex with Novel T State Inhibitors at 2.50 Resolution 提交 2006-02-09	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	EOZ 3,5-BIS[(PHOSPHONOACETYL)AMINO]BENZOIC ACID × 6 ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；ATCase holoenzyme was crystallized by microdialysis, using 50 L wells. The enzyme solution, at ~18 mg/mL, was dialyzed against a solution of 40 mM citric acid, 3 mM sodium azide, 1 mM 2-mercaptoethanol, 1 mM cytidine 5 -triphosphate, 0.2 mM EDTA (pH 5.7), MICRODIALYSIS, temperature 298K	分辨率 2.50 Å R-free 0.239
2H3E Structure of wild-type E. coli Aspartate Transcarbamoylase in the presence of N-phosphonacetyl-L-isoasparagine at 2.3A resolution 提交 2006-05-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	6PR (S)-4-AMINO-4-OXO-3-(2-PHOSPHONOACETAMIDO)BUTANOIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；293 K；50 mM Maleic acid, 3 mM sodium azide, 1 mM N-phosphonacetyl-L-isoasparagine, pH 5.7, MICRODIALYSIS, temperature 293K	分辨率 2.30 Å R-free 0.250
2HSE Structure of D236A E. coli Aspartate Transcarbamoylase in the presence of phosphonoacetamide and l-Aspartate at 2.60 A resolution 提交 2006-07-21	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PO4 PHOSPHATE ION × 3 PCT PHOSPHONOACETAMIDE × 6 ASP ASPARTIC ACID × 9 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.8；293 K；50mM maleic acid, 3mM sodium azide, 10mM phosphonoacetamide, 20mM L-aspartate, pH 5.8, MICRODIALYSIS, temperature 293K	分辨率 2.60 Å R-free 0.254
2IPO E. coli Aspartate Transcarbamoylase complexed with N-phosphonacetyl-L-asparagine 提交 2006-10-12	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	1IP N~2~-(PHOSPHONOACETYL)-L-ASPARAGINE × 6 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 6 ZN ZINC ION × 6 MAE MALEIC ACID × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；295 K；50 mM Maleic acid, 1 mM N-phosphonacetyl-L-asparagine, 3 mM Sodium Azide, pH 5.7, MICRODIALYSIS, temperature 295K	分辨率 2.60 Å R-free 0.253
2QG9 Structure of a regulatory subunit mutant D19A of ATCase from E. coli 提交 2007-06-28	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	突变：D19A 突变：D19A	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；0.1M citrate buffer, pH 5.7, MICRODIALYSIS, temperature 298.0K	分辨率 2.70 Å R-free 0.244
2QGF Structure of regulatory chain mutant H20A of asparate transcarbamoylase from E. coli 提交 2007-06-28	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	突变：H20A 突变：H20A	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；0.1M citric acid, pH 5.7, MICRODIALYSIS, temperature 298.0K	分辨率 2.20 Å R-free 0.223
3AT1 CRYSTAL STRUCTURES OF PHOSPHONOACETAMIDE LIGATED T AND PHOSPHONOACETAMIDE AND MALONATE LIGATED R STATES OF ASPARTATE CARBAMOYLTRANSFERASE AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL PH 提交 1989-09-22	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.80 Å
3D7S Crystal structure of Wild-Type E. Coli Asparate Transcarbamoylase at pH 8.5 at 2.80 A Resolution 提交 2008-05-21	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；50 mM Tris 8K PEG, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.80 Å R-free 0.235
3MPU Crystal structure of the C47A/A241C disulfide-linked E. coli Aspartate Transcarbamoylase holoenzyme 提交 2010-04-27	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	PO4 PHOSPHATE ION × 12 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.9；293 K；Protein at 10 mg/ml was dialyzed against solution containing 100 mM KH2PO4, 3mM NaN3, pH 5.9, MICRODIALYSIS, temperature 293K	分辨率 2.85 Å R-free 0.240
3MPU Crystal structure of the C47A/A241C disulfide-linked E. coli Aspartate Transcarbamoylase holoenzyme 提交 2010-04-27	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 F 1–153（153 aa）	未记录	PO4 PHOSPHATE ION × 12 ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.9；293 K；Protein at 10 mg/ml was dialyzed against solution containing 100 mM KH2PO4, 3mM NaN3, pH 5.9, MICRODIALYSIS, temperature 293K	分辨率 2.85 Å R-free 0.240
4AT1 STRUCTURAL CONSEQUENCES OF EFFECTOR BINDING TO THE T STATE OF ASPARTATE CARBAMOYLTRANSFERASE. CRYSTAL STRUCTURES OF THE UNLIGATED AND ATP-, AND CTP-COMPLEXED ENZYMES AT 2.6-ANGSTROMS RESOLUTION 提交 1990-04-26	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.60 Å
4E2F Crystal Structure of E. coli Aspartate Transcarbamoylase K164E/E239K Mutant in an intermediate state 提交 2012-03-08	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）链 F 1–153（153 aa）链 H 1–153（153 aa）链 J 1–153（153 aa）链 L 1–153（153 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8.75；293 K；16% (w/v) PEG 4000, 0.04 M Na2MoO4, 0.04 M N-cyclohexyl-3-aminopropanesulfonic acid, 30 mM Tris-acetate, pH 8.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 2.80 Å R-free 0.274
4F04 A Second Allosteric site in E. coli Aspartate Transcarbamoylase: R-state ATCase with UTP bound 提交 2012-05-03	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6 UTP URIDINE 5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.95；298 K；50 mM maleic acid, 3 mM sodium azide, 1 mM PALA, adjusted to pH=5.95 with N-ethylmorpholine, MICRODIALYSIS, temperature 298K	分辨率 2.30 Å R-free 0.207
4FYV Aspartate Transcarbamoylase Complexed with dCTP 提交 2012-07-05	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	PO4 PHOSPHATE ION × 3 ZN ZINC ION × 6 DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；40 mM sodium citrate, 1 mM 2-mercaptoethanol, 0.2 mM EDTA, 1.0 mM dCTP, pH 5.7, MICRODIALYSIS, temperature 298K	分辨率 2.10 Å R-free 0.215
4FYW E. coli Aspartate Transcarbamoylase complexed with CTP 提交 2012-07-05	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；40 mM sodium citrate, 1 mM 2-mercaptoethanol, 0.2 mM EDTA, 1.0 mM CTP, pH 5.7, MICRODIALYSIS, temperature 298K	分辨率 2.10 Å R-free 0.229
4FYX E. coli Aspartate Transcarbamoylase complexed with dCTP, UTP, and Mg2+ 提交 2012-07-05	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 UTP URIDINE 5'-TRIPHOSPHATE × 6 DCP 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；40 mM sodium citrate, 1 mM 2-mercaptoethanol, 0.2 mM EDTA, 1.0 mM dCTP, pH 5.7 (crystals soaked in 5 mM UTP and 5 mM Mg2+), MICRODIALYSIS, temperature 298K	分辨率 2.09 Å R-free 0.212
4FYY E. coli Aspartate Transcarbamoylase Complexed with CTP, UTP, and Mg2+ 提交 2012-07-05	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）	未记录	ZN ZINC ION × 6 UTP URIDINE 5'-TRIPHOSPHATE × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6	X-RAY DIFFRACTION X-ray结晶条件 MICRODIALYSIS；pH 5.7；298 K；40 mM sodium citrate, 1 mM 2-mercaptoethanol, 0.2 mM EDTA, 1.0 mM CTP, pH 5.7 (crystals soaked in 5 mM UTP and 5 mM Mg2+), MICRODIALYSIS, temperature 298K	分辨率 1.94 Å R-free 0.236
5AT1 STRUCTURAL CONSEQUENCES OF EFFECTOR BINDING TO THE T STATE OF ASPARTATE CARBAMOYLTRANSFERASE. CRYSTAL STRUCTURES OF THE UNLIGATED AND ATP-, AND CTP-COMPLEXED ENZYMES AT 2.6-ANGSTROMS RESOLUTION 提交 1990-04-26	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.60 Å
6AT1 STRUCTURAL CONSEQUENCES OF EFFECTOR BINDING TO THE T STATE OF ASPARTATE CARBAMOYLTRANSFERASE. CRYSTAL STRUCTURES OF THE UNLIGATED AND ATP-, AND CTP-COMPLEXED ENZYMES AT 2.6-ANGSTROMS RESOLUTION 提交 1990-04-26	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.60 Å
6KJ8 E. coli ATCase holoenzyme mutant - G166P (catalytic chain) 提交 2019-07-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）链 F 1–153（153 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；0.1M HEPES pH 7.0, 30% Jeffamine M-600 pH 7.0, and 10% glycerol	分辨率 3.01 Å R-free 0.247
6KJA E. coli ATCase holoenzyme mutant - G128/130A (catalytic chain) 提交 2019-07-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）链 D 1–153（153 aa）链 F 1–153（153 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；0.1 M HEPES pH 7.0, 30% Jeffamine M-600 pH 7.0, and 10% glycerol	分辨率 3.06 Å R-free 0.283
6KJB wild-type apo-form E. coli ATCase holoenzyme with an unusual open conformation of R167 提交 2019-07-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–153（153 aa）	未记录	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；0.1M MES pH 6.0 10% Glycerol and 10% PEG 8000	分辨率 2.06 Å R-free 0.223
7AT1 CRYSTAL STRUCTURES OF ASPARTATE CARBAMOYLTRANSFERASE LIGATED WITH PHOSPHONOACETAMIDE, MALONATE, AND CTP OR ATP AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL P*H 提交 1989-09-22	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 3	X-RAY DIFFRACTION X-ray结晶条件 THE CRYSTALS WERE GROWN IN A SOLUTION OF PHOSPHONACETAMIDE (PAM) AND MALONATE (MAL) AT PH 5.8. THEY WERE THEN SOAKED IN A SOLUTION CONTAINING PAM, MAL, AND ATP AT PH 7.0.	分辨率 2.80 Å
8AT1 CRYSTAL STRUCTURES OF ASPARTATE CARBAMOYLTRANSFERASE LIGATED WITH PHOSPHONOACETAMIDE, MALONATE, AND CTP OR ATP AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL P*H 提交 1989-09-22	聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PCT PHOSPHONOACETAMIDE × 6 ZN ZINC ION × 6 CTP CYTIDINE-5'-TRIPHOSPHATE × 6	X-RAY DIFFRACTION X-ray结晶条件 THE CRYSTALS WERE GROWN IN A SOLUTION OF PHOSPHONACETAMIDE (PAM) AND MALONATE (MAL) AT PH 5.8. THEY WERE THEN SOAKED IN A SOLUTION CONTAINING PAM, MAL, AND CTP AT PH 7.0.	分辨率 2.80 Å
8ATC COMPLEX OF N-PHOSPHONACETYL-L-ASPARTATE WITH ASPARTATE CARBAMOYLTRANSFERASE. X-RAY REFINEMENT, ANALYSIS OF CONFORMATIONAL CHANGES AND CATALYTIC AND ALLOSTERIC MECHANISMS 提交 1989-08-25	配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 1–152（152 aa）链 D 1–152（152 aa）	未记录	PAL N-(PHOSPHONACETYL)-L-ASPARTIC ACID × 6 ZN ZINC ION × 6	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.50 Å
9ATC ATCASE Y165F MUTANT 提交 1998-06-26	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 12 PDB 声明：dodecameric	链 B 7–152（146 aa）	突变：CHAIN A, Y165F	ZN ZINC ION × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 6.3；pH 6.3	分辨率 2.40 Å R-free 0.344

共 54 个其他 PDB 条目、55 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	PYRI_ECOLI
Isoform	—
PDB实体	2
链与序列区间	作者链 B； PDB构建体 2–153； UniProt 1–152 作者链 D； PDB构建体 2–153； UniProt 1–152

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1tug
沉积日期 deposition_date	2004-06-24
结构标题 title	Aspartate Transcarbamoylase Catalytic Chain Mutant E50A Complex with Phosphonoacetamide, Malonate, and Cytidine-5-Prime-Triphosphate (CTP)
关键词 keywords	protein structure-function, site specific mutagenesis, domain closure, allosteric transition, HYDROLASE-HYDROLASE REGULATOR COMPLEX; HYDROLASE/HYDROLASE REGULATOR
实验方法 method	X-RAY DIFFRACTION

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	37.69 Å
回转半径 Rg (电子) rg_electron	37.11 Å
零角强度 I(0) i0	171475000.00
分子量 molecular_weight	104290.0 kDa
排除体积 excluded_volume	130010 Å³
包络体积 envelope_volume	174780 Å³
水化壳体积 shell_volume	39768 Å³
包络直径 envelope_diameter	109.1 Å
壳层 Rg shell_rg	43.02 Å
包络 Rg envelope_rg	35.86 Å
形状 Rg shape_rg	37.12 Å
总 Rg total_rg	37.45 Å
总原子数 total_atoms	7314
残基数 n_residues	926
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	104.0 Å
Rg (实空间) rg_real	37.56 Å
Rg 误差 (实空间) rg_real_error	0.65 Å
I(0) (实空间) i0_real	1.7150e+08
I(0) 误差 (实空间) i0_real_error	2.9180e+06
Rg (倒空间) rg_reciprocal	37.65 Å
I(0) (倒空间) i0_reciprocal	171500000.0000
解质量估计 total_estimate	0.8381
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	61.9 Å
偏度 Skewness skewness	0.024
峰度 Kurtosis kurtosis	-0.907
角度范围 angular_range	— – 0.2100 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	19350000.0000
实空间数据点数 n_real_points	43
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.990; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.920; Smooth: 0.001

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (7)

实体 1 Entity 1 polymer

描述 description	Aspartate carbamoyltransferase catalytic chain
分子量 formula_weight	34279.1 kDa
EC 编号 ec_number	2.1.3.2
突变 mutation	E50A
来源方法 src_method	man
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,C
原子数 atom_count	8012

ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFAASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL

来源 Source

来源生物 organism	Escherichia coli
NCBI 分类编号 ncbi_taxonomy_id	562
宿主生物 host_organism	Escherichia coli

实体 2 Entity 2 polymer

描述 description	Aspartate carbamoyltransferase regulatory chain
分子量 formula_weight	17143.6 kDa
来源方法 src_method	man
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	B,D

MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITIGLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPERIDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN

来源 Source

来源生物 organism	Escherichia coli
NCBI 分类编号 ncbi_taxonomy_id	562
宿主生物 host_organism	Escherichia coli

实体 3 Entity 3 non-polymer

描述 description	MALONATE ION
分子量 formula_weight	102.0 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 4 Entity 4 non-polymer

描述 description	PHOSPHONOACETAMIDE
分子量 formula_weight	139.0 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 5 Entity 5 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 6 Entity 6 non-polymer

描述 description	CYTIDINE-5'-TRIPHOSPHATE
分子量 formula_weight	483.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 7 Entity 7 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	698

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7. 折叠分类 (SCOP + CATH) 14 domains

SCOP 2.08 (6 domains)

结构域编号 domain_id	d1tuga1
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.78 — ATC-like
超家族 Superfamily superfamily	c.78.1 — Aspartate/ornithine carbamoyltransferase
家族 Family family	c.78.1.1 — Aspartate/ornithine carbamoyltransferase

结构域编号 domain_id	d1tugb1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.2 — Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain
家族 Family family	d.58.2.1 — Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain

结构域编号 domain_id	d1tugb2
类 Class class	g — Small proteins
折叠类型 Fold fold	g.41 — Rubredoxin-like
超家族 Superfamily superfamily	g.41.7 — Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain
家族 Family family	g.41.7.1 — Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain

结构域编号 domain_id	d1tugc1
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.78 — ATC-like
超家族 Superfamily superfamily	c.78.1 — Aspartate/ornithine carbamoyltransferase
家族 Family family	c.78.1.1 — Aspartate/ornithine carbamoyltransferase

结构域编号 domain_id	d1tugd1
类 Class class	d — Alpha and beta proteins (a+b)
折叠类型 Fold fold	d.58 — Ferredoxin-like
超家族 Superfamily superfamily	d.58.2 — Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain
家族 Family family	d.58.2.1 — Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain

结构域编号 domain_id	d1tugd2
类 Class class	g — Small proteins
折叠类型 Fold fold	g.41 — Rubredoxin-like
超家族 Superfamily superfamily	g.41.7 — Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain
家族 Family family	g.41.7.1 — Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain

CATH v4.4 (8 domains)

结构域编号 domain_id	1tugA01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	1370 — Aspartate/ornithine carbamoyltransferase

结构域编号 domain_id	1tugA02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	1370 — Aspartate/ornithine carbamoyltransferase

结构域编号 domain_id	1tugB01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	140 — Aspartate carbamoyltransferase regulatory subunit, N-terminal domain

结构域编号 domain_id	1tugB02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	20 — Aspartate carbamoyltransferase regulatory subunit, C-terminal domain

结构域编号 domain_id	1tugC01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	1370 — Aspartate/ornithine carbamoyltransferase

结构域编号 domain_id	1tugC02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	1370 — Aspartate/ornithine carbamoyltransferase

结构域编号 domain_id	1tugD01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily	140 — Aspartate carbamoyltransferase regulatory subunit, N-terminal domain

结构域编号 domain_id	1tugD02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	20 — Aspartate carbamoyltransferase regulatory subunit, C-terminal domain

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8. 引用文献 (1)

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晶胞边长 a length_a	122.290 Å
晶胞边长 b length_b	122.290 Å
晶胞边长 c length_c	142.410 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	120.00 °
晶胞分子数 Z z_pdb	12

空间群 space_group	P 3 2 1
空间群编号 int_tables_number	150
晶系 crystal_system	—

分辨率 resolution	2.100 Å
最低分辨率 resolution_low	30.000 Å
R 因子 r_factor	0.2196
R_work r_work	—
R_free r_free	0.2719

fit	/app/data/saxs_computation/tu/1tug/1tug.fit
pddf	/app/data/pr_computation/tu/1tug/1tug.pr.out
profile	/app/data/saxs_computation/tu/1tug/1tug.dat

Aspartate Transcarbamoylase Catalytic Chain Mutant E50A Complex with Phosphonoacetamide, Malonate, and Cytidine-5-Prime-Triphosphate (CTP)

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Aspartate carbamoyltransferase catalytic chain

当前结构中的状态

数据库中的同蛋白其他状态

Aspartate carbamoyltransferase regulatory chain

当前结构中的状态

数据库中的同蛋白其他状态

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

2. 结构基本信息 2. Structure Basics

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

5. 晶体学与实验 5. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

6. 实体与聚合物信息 Entities & Polymers (7)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 polymer

聚合物 Polymer

来源 Source

实体 3 Entity 3 non-polymer

实体 4 Entity 4 non-polymer

实体 5 Entity 5 non-polymer

实体 6 Entity 6 non-polymer

实体 7 Entity 7 water

7. 折叠分类 (SCOP + CATH) 14 domains

SCOP 2.08 (6 domains)

CATH v4.4 (8 domains)

8. 引用文献 (1)

9. 文件与曲线 (10)

Files (7)

Curves (3)