SAXSdb — 1uwk

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1. Protein Identity and Related Structures Protein Identity & Related Structures

UROCANATE HYDRATASE

PSEUDOMONAS PUTIDA

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 2	NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 2 (2E)-3-(1H-IMIDAZOL-4-YL)ACRYLIC ACID × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	HUTU_PSEPU
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–557； UniProt 0–556 Author chain B； PDBConstruct 1–557； UniProt 0–556

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1uwk
Deposition date deposition_date	2004-02-05
Structure title title	The High Resolution Structure of Urocanate Hydratase from Pseudomonas putida in complex with urocanate
Keywords keywords	HYDROLASE, UROCANASE, IMIDAZOLONEPROPIONATE, HISTIDINE METABOLISM, LYASE; HYDROLASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1uwk__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1uwk__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

1uwk__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	30.36 Å
Rg (electron density)	29.75 Å
Total Rg	30.42 Å
Atom count	8587
Residues	1107
Excluded volume	151200 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`1uwk__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Description description	UROCANATE HYDRATASE
Molecular weight formula_weight	60757.5 kDa
EC 编号 ec_number	4.2.1.49
Mutation mutation	YES
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	9437

MTDNNKYRDVEIRAPRGNKLTAKSWLTEAPLRMLMNNLDPQVAENPKELVVYGGIGRAARNWECYDKIVETLTRLEDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVPHWANWEHFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYGGSLKGKWVLTAGLGGMGGAQPLAATLAGACSLNIESQQSRIDFRLETRYVDEQATDLDDALVRIAKYTAEGKAISIALHGNAAEILPELVKRGVRPDMVTDQTSAHDPLNGYLPAGWTWEQYRDRAQTEPAAVVKAAKQSMAVHVQAMLDFQKQGVPTFDYGNNIRQMAKEEGVADAFDFPGFVPAYIRPLFCRGVGPFRWAALSGEAEDIYKTDAKVKELIPDDAHLHRWLDMARERISFQGLPARICWVGLGLRAKLGLAFNEMVRSGELSAPVVIGRDHLDSGSVSSPNAETEAMRDGSDAVSDWPLLNALLNTAGGATWVSLHHGGGVGMGFSQHSGMVIVCDGTDEAAERIARVLTNDPGTGVMRHADAGYDIAIDCAKEQGLDLPMITG

Source Source

Source organism organism	PSEUDOMONAS PUTIDA
NCBI taxonomy ID ncbi_taxonomy_id	303
Host organism host_organism	ESCHERICHIA COLI

Entity 2 Entity 2 non-polymer

Description description	NICOTINAMIDE-ADENINE-DINUCLEOTIDE
Molecular weight formula_weight	663.4 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 3 Entity 3 non-polymer

Description description	(2E)-3-(1H-IMIDAZOL-4-YL)ACRYLIC ACID
Molecular weight formula_weight	138.1 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 4 Entity 4 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	840

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6. Fold Classification (SCOP + CATH) 6 domains

SCOP 2.08 (2 domains)

Domain ID domain_id	d1uwka_
Class class	e — Multi-domain proteins (alpha and beta)
Fold Fold fold	e.51 — Urocanase
Superfamily Superfamily superfamily	e.51.1 — Urocanase
Family Family family	e.51.1.1 — Urocanase

Domain ID domain_id	d1uwkb_
Class class	e — Multi-domain proteins (alpha and beta)
Fold Fold fold	e.51 — Urocanase
Superfamily Superfamily superfamily	e.51.1 — Urocanase
Family Family family	e.51.1.1 — Urocanase

CATH v4.4 (4 domains)

Domain ID domain_id	1uwkA01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	1770 — Urocanase fold
Homologous superfamily homologous superfamily	10 — Urocanase superfamily

Domain ID domain_id	1uwkA02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	10730 — Urocanase like domains

Domain ID domain_id	1uwkB01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	1770 — Urocanase fold
Homologous superfamily homologous superfamily	10 — Urocanase superfamily

Domain ID domain_id	1uwkB02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	10730 — Urocanase like domains

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Unit-cell length a length_a	111.429 Å
Unit-cell length b length_b	71.618 Å
Unit-cell length c length_c	129.158 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	98.75 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	C 1 2 1
Space-group number int_tables_number	5
Crystal system crystal_system	—

Resolution resolution	1.190 Å
Low-resolution limit resolution_low	50.000 Å
R factor r_factor	0.1396
R_work r_work	—
R_free r_free	0.1790

The High Resolution Structure of Urocanate Hydratase from Pseudomonas putida in complex with urocanate

1. Protein Identity and Related Structures Protein Identity & Related Structures

UROCANATE HYDRATASE

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1uwk__assembly_1__model_1

1uwk__assembly_1__model_1 | I(q)

1uwk__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 water

6. Fold Classification (SCOP + CATH) 6 domains

SCOP 2.08 (2 domains)

CATH v4.4 (4 domains)

7. Citations (1)

The High Resolution Structure of Urocanate Hydratase from Pseudomonas putida in complex with urocanate

1. Protein Identity and Related Structures Protein Identity & Related Structures

UROCANATE HYDRATASE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

1uwk__assembly_1__model_1

1uwk__assembly_1__model_1 | I(q)

1uwk__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (4)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 water

6. Fold Classification (SCOP + CATH) 6 domains

SCOP 2.08 (2 domains)

CATH v4.4 (4 domains)

7. Citations (1)