1wxw

Crystal structure of Tt1595, a putative SAM-dependent methyltransferase from Thermus thermophillus HB8

Method: X-RAY DIFFRACTION

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

hypothetical protein TTHA1280

Thermus thermophilus

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	蛋白同源多聚体	同源多聚体	蛋白 2	water × 2	与蛋白数一致
2	蛋白同源多聚体	同源多聚体	蛋白 2	HEXANE-1,6-DIOL × 2 water × 2	与蛋白数一致
3	蛋白同源多聚体	同源多聚体	蛋白 2	water × 2	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	Q5SIT4_THET8
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–382； UniProt 1–382 作者链 B； PDB构建体 1–382； UniProt 1–382 作者链 C； PDB构建体 1–382； UniProt 1–382 作者链 D； PDB构建体 1–382； UniProt 1–382

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1wxw
沉积日期 deposition_date	2005-02-02
结构标题 title	Crystal structure of Tt1595, a putative SAM-dependent methyltransferase from Thermus thermophillus HB8
关键词 keywords	;Thermus thermophillus, methyltransferase, Adomet, structural genomics, NPPSFA, National Project on Protein Structural and Functional Analyses, RIKEN Structural Genomics/Proteomics Initiative, RSGI, TRANSFERASE ;; TRANSFERASE
实验方法 method	X-RAY DIFFRACTION

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3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

1wxw__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

1wxw__assembly_1__model_1 | I(q)

100 个绘图点；横轴和纵轴均采用对数尺度。

1wxw__assembly_1__model_1 | P(r) · Pending

P(r) 将按相同 assembly/model 分别计算和展示。

Rg（Guinier）	29.19 Å
Rg（电子密度）	28.18 Å
总 Rg	29.01 Å
原子数	6053
残基数	751
排除体积	107650 Å³
最大 q	0.500 Å⁻¹

Assembly	Model	结构单元	聚集说明	状态	CRYSOL	操作
1	1	`1wxw__assembly_1__model_1`	dimeric （2）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
2	1	`1wxw__assembly_2__model_1`	dimeric （2）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
3	1	`1wxw__assembly_3__model_1`	dimeric （2）	成功	4.1.3-1-20251215 (887e7ef)	查看下载

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合物信息 Entities & Polymers (3)

实体 1 Entity 1 polymer

描述 description	hypothetical protein TTHA1280
分子量 formula_weight	43252.9 kDa
来源方法 src_method	man
拷贝数 entity_count	4

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D
原子数 atom_count	12185

MRIQVNAKGAARLLSRHLWVFRRDVVSGPETPGLYPVYWGRRFLALALYNPHTDLAVRAYRFAPAEDPVAALLENLAQALARREAVLRQDPEGGYRLVHAEGDLLPGLVVDYYAGHAVVQATAHAWEGLLPQVAEALRPHVQSVLAKNDARTRELEGLPLYVRPLLGEVPERVQVQEGRVRYLVDLRAGQKTGAYLDQRENRLYMERFRGERALDVFSYAGGFALHLALGFREVVAVDSSAEALRRAEENARLNGLGNVRVLEANAFDLLRRLEKEGERFDLVVLDPPAFAKGKKDVERAYRAYKEVNLRAIKLLKEGGILATASCSHHMTEPLFYAMVAEAAQDAHRLLRVVEKRGQPFDHPVLLNHPETHYLKFAVFQVL

来源 Source

来源生物 organism	Thermus thermophilus
NCBI 分类编号 ncbi_taxonomy_id	300852
宿主生物 host_organism	Escherichia coli

实体 2 Entity 2 non-polymer

描述 description	HEXANE-1,6-DIOL
分子量 formula_weight	118.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

实体 3 Entity 3 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	38

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6. 折叠分类 (SCOP + CATH) 20 domains

SCOP 2.08 (8 domains)

结构域编号 domain_id	d1wxwa1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.122 — PUA domain-like
超家族 Superfamily superfamily	b.122.1 — PUA domain-like
家族 Family family	b.122.1.9 — Hypothetical RNA methyltransferase domain (HRMD)

结构域编号 domain_id	d1wxwa2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.66 — S-adenosyl-L-methionine-dependent methyltransferases
超家族 Superfamily superfamily	c.66.1 — S-adenosyl-L-methionine-dependent methyltransferases
家族 Family family	c.66.1.51 — hypothetical RNA methyltransferase

结构域编号 domain_id	d1wxwb1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.122 — PUA domain-like
超家族 Superfamily superfamily	b.122.1 — PUA domain-like
家族 Family family	b.122.1.9 — Hypothetical RNA methyltransferase domain (HRMD)

结构域编号 domain_id	d1wxwb2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.66 — S-adenosyl-L-methionine-dependent methyltransferases
超家族 Superfamily superfamily	c.66.1 — S-adenosyl-L-methionine-dependent methyltransferases
家族 Family family	c.66.1.51 — hypothetical RNA methyltransferase

结构域编号 domain_id	d1wxwc1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.122 — PUA domain-like
超家族 Superfamily superfamily	b.122.1 — PUA domain-like
家族 Family family	b.122.1.9 — Hypothetical RNA methyltransferase domain (HRMD)

结构域编号 domain_id	d1wxwc2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.66 — S-adenosyl-L-methionine-dependent methyltransferases
超家族 Superfamily superfamily	c.66.1 — S-adenosyl-L-methionine-dependent methyltransferases
家族 Family family	c.66.1.51 — hypothetical RNA methyltransferase

结构域编号 domain_id	d1wxwd1
类 Class class	b — All beta proteins
折叠类型 Fold fold	b.122 — PUA domain-like
超家族 Superfamily superfamily	b.122.1 — PUA domain-like
家族 Family family	b.122.1.9 — Hypothetical RNA methyltransferase domain (HRMD)

结构域编号 domain_id	d1wxwd2
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.66 — S-adenosyl-L-methionine-dependent methyltransferases
超家族 Superfamily superfamily	c.66.1 — S-adenosyl-L-methionine-dependent methyltransferases
家族 Family family	c.66.1.51 — hypothetical RNA methyltransferase

CATH v4.4 (12 domains)

结构域编号 domain_id	1wxwA01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	130 — Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — PUA domain

结构域编号 domain_id	1wxwA02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	750 — Transcription Regulator spoIIAA
同源超家族 H-superfamily homologous superfamily	80 — RNA methyltransferase domain (HRMD) like

结构域编号 domain_id	1wxwA03
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	150 — Vaccinia Virus protein VP39

结构域编号 domain_id	1wxwB01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	130 — Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — PUA domain

结构域编号 domain_id	1wxwB02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	750 — Transcription Regulator spoIIAA
同源超家族 H-superfamily homologous superfamily	80 — RNA methyltransferase domain (HRMD) like

结构域编号 domain_id	1wxwB03
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	150 — Vaccinia Virus protein VP39

结构域编号 domain_id	1wxwC01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	130 — Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — PUA domain

结构域编号 domain_id	1wxwC02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	750 — Transcription Regulator spoIIAA
同源超家族 H-superfamily homologous superfamily	80 — RNA methyltransferase domain (HRMD) like

结构域编号 domain_id	1wxwC03
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	150 — Vaccinia Virus protein VP39

结构域编号 domain_id	1wxwD01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	130 — Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 4
同源超家族 H-superfamily homologous superfamily	10 — PUA domain

结构域编号 domain_id	1wxwD02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	30 — 2-Layer Sandwich
拓扑 Topology topology	750 — Transcription Regulator spoIIAA
同源超家族 H-superfamily homologous superfamily	80 — RNA methyltransferase domain (HRMD) like

结构域编号 domain_id	1wxwD03
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	150 — Vaccinia Virus protein VP39

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晶胞边长 a length_a	252.704 Å
晶胞边长 b length_b	45.962 Å
晶胞边长 c length_c	139.640 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	100.08 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	16

空间群 space_group	C 1 2 1
空间群编号 int_tables_number	5
晶系 crystal_system	—

分辨率 resolution	2.550 Å
最低分辨率 resolution_low	45.830 Å
R 因子 r_factor	0.2230
R_work r_work	0.2230
R_free r_free	0.2790

Crystal structure of Tt1595, a putative SAM-dependent methyltransferase from Thermus thermophillus HB8

1. 蛋白身份与相关结构 Protein Identity & Related Structures

hypothetical protein TTHA1280

当前结构中的状态

数据库中的同蛋白其他状态

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1wxw__assembly_1__model_1

1wxw__assembly_1__model_1 | I(q)

1wxw__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (3)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

实体 3 Entity 3 water

6. 折叠分类 (SCOP + CATH) 20 domains

SCOP 2.08 (8 domains)

CATH v4.4 (12 domains)

7. 引用文献 (1)

Crystal structure of Tt1595, a putative SAM-dependent methyltransferase from Thermus thermophillus HB8

1. 蛋白身份与相关结构 Protein Identity & Related Structures

hypothetical protein TTHA1280

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

1wxw__assembly_1__model_1

1wxw__assembly_1__model_1 | I(q)

1wxw__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (3)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

实体 3 Entity 3 water

6. 折叠分类 (SCOP + CATH) 20 domains

SCOP 2.08 (8 domains)

CATH v4.4 (12 domains)

7. 引用文献 (1)