2ep8

Solution structure of the BRCT domain from human Pescadillo homolog 1

Method: SOLUTION NMR Dmax: 60.5 Å Quality: REASONABLE

1. Protein Identity and Related Structures Protein Identity & Related Structures

Pescadillo homolog 1

Homo sapiens

UniProt O00541

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric(1) Consistent with protein copy count Chain A; UniProt 322–414 Fragment:BRCT domain No other associated polymer SOLUTION NMR NMR measurement conditions:pH 7;293 K;Ionic strength (raw mmCIF value) 120mM;Pressure ambient NMR sample composition:0.46mM uniformly 13C,15N-labeled protein; 20mM TrisHCl, 100mM NaCl, 1mM DTT, 0.02% NaN3; 10% D2O, 90% H2O | 10% D2O, 90% H2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

25 other PDB entries and 25 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PESC_HUMAN
Isoform
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 8–100; UniProt 322–414

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2ep8

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2ep8
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2. Structure Basics 2. Structure Basics

Entry ID entry_id2ep8
Deposition date deposition_date2007-03-29
Structure title titleSolution structure of the BRCT domain from human Pescadillo homolog 1
Keywords keywords;a/b/a 3 layers, nucleolus, ribosome biogenesis, DNA damage, Structural Genomics, NPPSFA, National Project on Protein Structural and Functional Analyses, RIKEN Structural Genomics/Proteomics Initiative, RSGI, CELL CYCLE ;; CELL CYCLE
Experimental Method methodSOLUTION NMR

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier14.45
Radius of gyration Rg (electron density) rg_electron13.89
Forward intensity I(0) i0673888000.00
Molecular weight molecular_weight224180.0 kDa
Excluded volume excluded_volume282210 ų
Envelope volume envelope_volume30949 ų
Hydration-shell volume shell_volume15239 ų
Envelope diameter envelope_diameter64.3
Shell Rg shell_rg23.64
Envelope Rg envelope_rg19.16
Shape Rg shape_rg13.82
Total Rg total_rg14.34
Total atoms total_atoms31560
Residues n_residues2000
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 −1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax60.5
Rg (real space) rg_real14.52
Rg uncertainty (real space) rg_real_error0.68
I(0) (real space) i0_real6.7390e+08
I(0) uncertainty (real space) i0_real_error8.5850e+06
Rg (reciprocal space) rg_reciprocal14.51
I(0) (reciprocal space) i0_reciprocal673900000.0000
Solution quality estimate total_estimate0.7159
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary16.9
Skewness Skewness skewness0.582
Kurtosis Kurtosis kurtosis0.555
Angular range angular_range— – 0.5000 −1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha303200.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.264; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.516; Smooth: 0.997

5. Crystallography and Experiment 5. Crystallography & Experiment

6. Entities and Polymers Entities & Polymers (1)

7. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id2ep8A01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily10190 — BRCT domain

8. Citations (1)

9. Files and Curves (10)