2h50
Multiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
1. 结构基本信息 1. Structure Basics
| 条目编号 entry_id | 2h50 |
| 沉积日期 deposition_date | 2006-05-25 |
| 结构标题 title | Multiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26 |
| 关键词 keywords | alpha-crystallin, chaperones, heat shock proteins, single particle reconstruction, chaperone; CHAPERONE |
| 实验方法 method | ELECTRON MICROSCOPY |
2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | 81.26 Å |
| 回转半径 Rg (电子) rg_electron | 80.45 Å |
| 零角强度 I(0) i0 | 921092000.00 |
| 分子量 molecular_weight | 255390.0 kDa |
| 排除体积 excluded_volume | 320030 ų |
| 包络体积 envelope_volume | 988050 ų |
| 水化壳体积 shell_volume | 106200 ų |
| 包络直径 envelope_diameter | 188.6 Å |
| 壳层 Rg shell_rg | 88.73 Å |
| 包络 Rg envelope_rg | 67.48 Å |
| 形状 Rg shape_rg | 80.43 Å |
| 总 Rg total_rg | 80.66 Å |
| 总原子数 total_atoms | 18000 |
| 残基数 n_residues | 2232 |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 191.8 Å |
| Rg (实空间) rg_real | 80.39 Å |
| Rg 误差 (实空间) rg_real_error | 0.41 Å |
| I(0) (实空间) i0_real | 9.2110e+08 |
| I(0) 误差 (实空间) i0_real_error | 1.6650e+07 |
| Rg (倒空间) rg_reciprocal | 84.25 Å |
| I(0) (倒空间) i0_reciprocal | 929100000.0000 |
| 解质量估计 total_estimate | 0.7420 |
| 解质量评级 solution_quality | REASONABLE a REASONABLE solution |
| P(r) 峰数 n_peaks | 2 |
| 主峰位置 r_peak_primary | 131.2 Å |
| 偏度 Skewness skewness | -0.633 |
| 峰度 Kurtosis kurtosis | -0.372 |
| 角度范围 angular_range | — – 0.0950 Å−1 |
| 当前正则化参数 α current_alpha | 0.0026 |
| 最高正则化参数 α highest_alpha | 281800000.0000 |
| 实空间数据点数 n_real_points | 20 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.567; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.942; Smooth: 0.000 |
4. 晶体学与实验 4. Crystallography & Experiment
5. 实体与聚合体信息 (1)
6. 折叠分类 (SCOP + CATH) 24 domains
SCOP 2.08 (24 domains)
| 结构域编号 domain_id | d2h50a1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50b1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50c1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50d1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50e1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50f1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50g1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50h1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50i1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50j1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50k1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50l1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50m1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50n1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50o1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50p1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50q1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50r1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50s1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50t1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50u1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50v1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50w1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |
| 结构域编号 domain_id | d2h50x1 |
| 类 Class class | b — All beta proteins |
| 折叠类型 Fold fold | b.15 — HSP20-like chaperones |
| 超家族 Superfamily superfamily | b.15.1 — HSP20-like chaperones |
| 家族 Family family | b.15.1.1 — HSP20 |