SAXSdb — 2h8c

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Crossover junction endodeoxyribonuclease rusA

Escherichia coli

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–DNA	Homooligomer	Protein 2 DNA 2	5'-D(CPCPGPGPTPAPCPCPGPGP*T)-3' × 2	Consistent with all polymers
2	Protein–DNA	Homooligomer	Protein 2 DNA 2	5'-D(CPCPGPGPTPAPCPCPGPGP*T)-3' × 2 water × 2	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RUS_ECOLI
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain A； PDBConstruct 1–120； UniProt 1–120 Author chain B； PDBConstruct 1–120； UniProt 1–120 Author chain C； PDBConstruct 1–120； UniProt 1–120 Author chain D； PDBConstruct 1–120； UniProt 1–120

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2h8c
Deposition date deposition_date	2006-06-07
Structure title title	Structure of RusA D70N in complex with DNA
Keywords keywords	PROTEIN-DNA COMPLEX, RECOMBINATION, HYDROLASE-DNA COMPLEX; HYDROLASE/DNA
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2h8c__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2h8c__assembly_2__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

2h8c__assembly_2__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	21.59 Å
Rg (electron density)	20.31 Å
Total Rg	21.23 Å
Atom count	2099
Residues	244
Excluded volume	35799 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2h8c__assembly_1__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`2h8c__assembly_2__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	5'-D(CPCPGPGPTPAPCPCPGPGP*T)-3'
Molecular weight formula_weight	3350.2 kDa
Source method src_method	syn
Copy count entity_count	4

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	Y,Z,W,X
Atom count atom_count	4357

CCGGTACCGGT

Entity 2 Entity 2 polymer

Description description	Crossover junction endodeoxyribonuclease rusA
Molecular weight formula_weight	13866.9 kDa
EC 编号 ec_number	3.1.22.-
Mutation mutation	D70N
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D

MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKNAMLDIGLAMPVKIRIECHMPDRRRRNLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKMPVTKGGRLELTITEMGNE

Source Source

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli

Entity 3 Entity 3 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	2

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6. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

Domain ID domain_id	d2h8ca_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.79 — Bacillus chorismate mutase-like
Superfamily Superfamily superfamily	d.79.6 — Holliday junction resolvase RusA
Family Family family	d.79.6.1 — Holliday junction resolvase RusA

Domain ID domain_id	d2h8cb_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.79 — Bacillus chorismate mutase-like
Superfamily Superfamily superfamily	d.79.6 — Holliday junction resolvase RusA
Family Family family	d.79.6.1 — Holliday junction resolvase RusA

Domain ID domain_id	d2h8cc_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.79 — Bacillus chorismate mutase-like
Superfamily Superfamily superfamily	d.79.6 — Holliday junction resolvase RusA
Family Family family	d.79.6.1 — Holliday junction resolvase RusA

Domain ID domain_id	d2h8cd_
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.79 — Bacillus chorismate mutase-like
Superfamily Superfamily superfamily	d.79.6 — Holliday junction resolvase RusA
Family Family family	d.79.6.1 — Holliday junction resolvase RusA

CATH v4.4 (4 domains)

Domain ID domain_id	2h8cA00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1330 — 60s Ribosomal Protein L30; Chain: A;
Homologous superfamily homologous superfamily	70 — Holliday junction resolvase RusA

Domain ID domain_id	2h8cB00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1330 — 60s Ribosomal Protein L30; Chain: A;
Homologous superfamily homologous superfamily	70 — Holliday junction resolvase RusA

Domain ID domain_id	2h8cC00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1330 — 60s Ribosomal Protein L30; Chain: A;
Homologous superfamily homologous superfamily	70 — Holliday junction resolvase RusA

Domain ID domain_id	2h8cD00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1330 — 60s Ribosomal Protein L30; Chain: A;
Homologous superfamily homologous superfamily	70 — Holliday junction resolvase RusA

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Unit-cell length a length_a	64.616 Å
Unit-cell length b length_b	59.483 Å
Unit-cell length c length_c	90.721 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	101.59 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	3.100 Å
Low-resolution limit resolution_low	44.460 Å
R factor r_factor	0.2490
R_work r_work	0.2470
R_free r_free	0.2890

Structure of RusA D70N in complex with DNA

1. Protein Identity and Related Structures Protein Identity & Related Structures

Crossover junction endodeoxyribonuclease rusA

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2h8c__assembly_2__model_1

2h8c__assembly_2__model_1 | I(q)

2h8c__assembly_2__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 water

6. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

CATH v4.4 (4 domains)

7. Citations (1)

Structure of RusA D70N in complex with DNA

1. Protein Identity and Related Structures Protein Identity & Related Structures

Crossover junction endodeoxyribonuclease rusA

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2h8c__assembly_2__model_1

2h8c__assembly_2__model_1 | I(q)

2h8c__assembly_2__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 water

6. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

CATH v4.4 (4 domains)

7. Citations (1)