2i00

Crystal structure of acetyltransferase (GNAT family) from Enterococcus faecalis

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Acetyltransferase, GNAT family

Enterococcus faecalis

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 2	water × 2	Consistent with protein count
2	Protein homooligomer	Homooligomer	Protein 2	water × 2	Consistent with protein count
3	Protein homooligomer	Homooligomer	Protein 2	water × 2	Consistent with protein count
4	Protein homooligomer	Homooligomer	Protein 6	water × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q831Y9_ENTFA
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–406； UniProt 1–406 Author chain B； PDBConstruct 1–406； UniProt 1–406 Author chain C； PDBConstruct 1–406； UniProt 1–406 Author chain D； PDBConstruct 1–406； UniProt 1–406 Author chain E； PDBConstruct 1–406； UniProt 1–406 Author chain F； PDBConstruct 1–406； UniProt 1–406

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2i00
Deposition date deposition_date	2006-08-09
Structure title title	Crystal structure of acetyltransferase (GNAT family) from Enterococcus faecalis
Keywords keywords	;acetyltransferase, GNAT family, structural genomics, PSI-2, Protein Structure Initiative, Midwest Center for Structural Genomics, MCSG, TRANSFERASE ;; TRANSFERASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2i00__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2i00__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

2i00__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	29.45 Å
Rg (electron density)	28.34 Å
Total Rg	29.14 Å
Atom count	6628
Residues	793
Excluded volume	117930 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2i00__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`2i00__assembly_2__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`2i00__assembly_3__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
4	1	`2i00__assembly_4__model_1`	hexameric （6）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Description description	Acetyltransferase, GNAT family
Molecular weight formula_weight	48077.1 kDa
EC 编号 ec_number	2.3.1.1
Source method src_method	man
Copy count entity_count	6

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F
Atom count atom_count	20278

MDEQEFRKQLTLKPVEEEHIDQFNELLSYVFQVTEADIEESGFENKRAFIKSKQPILELSKVFGWFHENQLISQIAIYPCEVNIHGALYKMGGVTGVGTYPEYANHGLMKDLIQTALEEMRQDKQWISYLFPYNIPYYRRKGWEIMSDKLSFKIRDTQLPKTVPVPGMIERLAVDHPDVFDVYARFARQNHGALIRSAFNWEEYWRFENEEERTAAVYYGANQEPLGVLFYWVADEVFHIKEMFYLNQEARNGLWNFITAHFSMVYWVKGDIYKNEPLAFLLEDSQIKESIEPYYMARIVDVKAFLENFPFESTAKPFHFVVKDPVAEWNNGIFGLIWDENDQVTITDEPLGTAVHLDIQTLTCLVMNYRRPSYLHRIERIDTDKETLNSLERIFPDQEAYFSDYF

Source Source

Source organism organism	Enterococcus faecalis
NCBI taxonomy ID ncbi_taxonomy_id	226185
Host organism host_organism	Escherichia coli BL21

Entity 2 Entity 2 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	376

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6. Fold Classification (SCOP + CATH) 30 domains

SCOP 2.08 (12 domains)

Domain ID domain_id	d2i00a1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.106 — SCP-like
Superfamily Superfamily superfamily	d.106.1 — SCP-like
Family Family family	d.106.1.4 — EF1021 C-terminal domain-like

Domain ID domain_id	d2i00a2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.108 — Acyl-CoA N-acyltransferases (Nat)
Superfamily Superfamily superfamily	d.108.1 — Acyl-CoA N-acyltransferases (Nat)
Family Family family	d.108.1.10 — EF1021-like

Domain ID domain_id	d2i00b1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.106 — SCP-like
Superfamily Superfamily superfamily	d.106.1 — SCP-like
Family Family family	d.106.1.4 — EF1021 C-terminal domain-like

Domain ID domain_id	d2i00b2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.108 — Acyl-CoA N-acyltransferases (Nat)
Superfamily Superfamily superfamily	d.108.1 — Acyl-CoA N-acyltransferases (Nat)
Family Family family	d.108.1.10 — EF1021-like

Domain ID domain_id	d2i00c1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.106 — SCP-like
Superfamily Superfamily superfamily	d.106.1 — SCP-like
Family Family family	d.106.1.4 — EF1021 C-terminal domain-like

Domain ID domain_id	d2i00c2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.108 — Acyl-CoA N-acyltransferases (Nat)
Superfamily Superfamily superfamily	d.108.1 — Acyl-CoA N-acyltransferases (Nat)
Family Family family	d.108.1.10 — EF1021-like

Domain ID domain_id	d2i00d1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.106 — SCP-like
Superfamily Superfamily superfamily	d.106.1 — SCP-like
Family Family family	d.106.1.4 — EF1021 C-terminal domain-like

Domain ID domain_id	d2i00d2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.108 — Acyl-CoA N-acyltransferases (Nat)
Superfamily Superfamily superfamily	d.108.1 — Acyl-CoA N-acyltransferases (Nat)
Family Family family	d.108.1.10 — EF1021-like

Domain ID domain_id	d2i00e1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.106 — SCP-like
Superfamily Superfamily superfamily	d.106.1 — SCP-like
Family Family family	d.106.1.4 — EF1021 C-terminal domain-like

Domain ID domain_id	d2i00e2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.108 — Acyl-CoA N-acyltransferases (Nat)
Superfamily Superfamily superfamily	d.108.1 — Acyl-CoA N-acyltransferases (Nat)
Family Family family	d.108.1.10 — EF1021-like

Domain ID domain_id	d2i00f1
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.106 — SCP-like
Superfamily Superfamily superfamily	d.106.1 — SCP-like
Family Family family	d.106.1.4 — EF1021 C-terminal domain-like

Domain ID domain_id	d2i00f2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.108 — Acyl-CoA N-acyltransferases (Nat)
Superfamily Superfamily superfamily	d.108.1 — Acyl-CoA N-acyltransferases (Nat)
Family Family family	d.108.1.10 — EF1021-like

CATH v4.4 (18 domains)

Domain ID domain_id	2i00A01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00A02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00A03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1050 — Nonspecific Lipid-transfer Protein; Chain A
Homologous superfamily homologous superfamily	10 — SCP2 sterol-binding domain

Domain ID domain_id	2i00B01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00B02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00B03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1050 — Nonspecific Lipid-transfer Protein; Chain A
Homologous superfamily homologous superfamily	10 — SCP2 sterol-binding domain

Domain ID domain_id	2i00C01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00C02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00C03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1050 — Nonspecific Lipid-transfer Protein; Chain A
Homologous superfamily homologous superfamily	10 — SCP2 sterol-binding domain

Domain ID domain_id	2i00D01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00D02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00D03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1050 — Nonspecific Lipid-transfer Protein; Chain A
Homologous superfamily homologous superfamily	10 — SCP2 sterol-binding domain

Domain ID domain_id	2i00E01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00E02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00E03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1050 — Nonspecific Lipid-transfer Protein; Chain A
Homologous superfamily homologous superfamily	10 — SCP2 sterol-binding domain

Domain ID domain_id	2i00F01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00F02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	630 — Aminopeptidase
Homologous superfamily homologous superfamily	30 — Gcn5-related N-acetyltransferase (GNAT)

Domain ID domain_id	2i00F03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	1050 — Nonspecific Lipid-transfer Protein; Chain A
Homologous superfamily homologous superfamily	10 — SCP2 sterol-binding domain

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Unit-cell length a length_a	77.410 Å
Unit-cell length b length_b	177.091 Å
Unit-cell length c length_c	104.429 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	94.89 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	12

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	2.300 Å
Low-resolution limit resolution_low	41.920 Å
R factor r_factor	0.2158
R_work r_work	0.2130
R_free r_free	0.2698

Crystal structure of acetyltransferase (GNAT family) from Enterococcus faecalis

1. Protein Identity and Related Structures Protein Identity & Related Structures

Acetyltransferase, GNAT family

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2i00__assembly_1__model_1

2i00__assembly_1__model_1 | I(q)

2i00__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 30 domains

SCOP 2.08 (12 domains)

CATH v4.4 (18 domains)

7. Citations (1)

Crystal structure of acetyltransferase (GNAT family) from Enterococcus faecalis

1. Protein Identity and Related Structures Protein Identity & Related Structures

Acetyltransferase, GNAT family

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2i00__assembly_1__model_1

2i00__assembly_1__model_1 | I(q)

2i00__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 30 domains

SCOP 2.08 (12 domains)

CATH v4.4 (18 domains)

7. Citations (1)