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2io8

E. coli Bifunctional glutathionylspermidine synthetase/amidase Incomplex with Mg2+ and ADP

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Bifunctional glutathionylspermidine synthetase/amidase

Escherichia coli

UniProt P0AES0

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein homooligomer Homooligomer Protein 2 MAGNESIUM ION × 4 CYSTEINE × 2 ADENOSINE-5'-DIPHOSPHATE × 2 water × 2 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
3a2y 3a2z 3a30

3 assemblies

Homodimer
2io7 2io8 2io9 2ioa 2iob 3a2y 3a2z 3a30 3o98

9 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex

None

View Construct and Data Evidence
UniProt name GSP_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–619； UniProt 1–619 Author chain B； PDBConstruct 1–619； UniProt 1–619

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2io8
Deposition date deposition_date2006-10-10
Structure title titleE. coli Bifunctional glutathionylspermidine synthetase/amidase Incomplex with Mg2+ and ADP
Keywords keywordsBifunctional glutathionylspermidine synthetase/amidase, LIGASE, HYDROLASE; LIGASE, HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2io8__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2io8__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2io8__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）34.63 Å
Rg (electron density)33.66 Å
Total Rg34.21 Å
Atom count9646
Residues1191
Excluded volume171130 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2io8__assembly_1__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

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6. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (6 domains)

Domain ID domain_idd2io8a1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.30 — PreATP-grasp domain
Superfamily Superfamily superfamilyc.30.1 — PreATP-grasp domain
Family Family familyc.30.1.7 — Glutathionylspermidine synthase substrate-binding domain-like
Domain ID domain_idd2io8a2
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.3 — Cysteine proteinases
Superfamily Superfamily superfamilyd.3.1 — Cysteine proteinases
Family Family familyd.3.1.15 — CHAP domain
Domain ID domain_idd2io8a3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.142 — ATP-grasp
Superfamily Superfamily superfamilyd.142.1 — Glutathione synthetase ATP-binding domain-like
Family Family familyd.142.1.8 — Glutathionylspermidine synthase ATP-binding domain-like
Domain ID domain_idd2io8b1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.30 — PreATP-grasp domain
Superfamily Superfamily superfamilyc.30.1 — PreATP-grasp domain
Family Family familyc.30.1.7 — Glutathionylspermidine synthase substrate-binding domain-like
Domain ID domain_idd2io8b2
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.3 — Cysteine proteinases
Superfamily Superfamily superfamilyd.3.1 — Cysteine proteinases
Family Family familyd.3.1.15 — CHAP domain
Domain ID domain_idd2io8b3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.142 — ATP-grasp
Superfamily Superfamily superfamilyd.142.1 — Glutathione synthetase ATP-binding domain-like
Family Family familyd.142.1.8 — Glutathionylspermidine synthase ATP-binding domain-like

CATH v4.4 (2 domains)

Domain ID domain_id2io8A01
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology1720 — endopeptidase fold (from Nostoc punctiforme)
Homologous superfamily homologous superfamily10 — endopeptidase domain like (from Nostoc punctiforme)
Domain ID domain_id2io8B01
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology1720 — endopeptidase fold (from Nostoc punctiforme)
Homologous superfamily homologous superfamily10 — endopeptidase domain like (from Nostoc punctiforme)
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7. Citations (1)