2k8x

;GlyTM1b(1-19)zip: A Chimeric Peptide Model of the N-Terminus of a Rat Short Alpha-Tropomyosin with the N-Terminus Encoded by Exon 1b in Complex with TM9d(252-284), a Peptide Model Containing the C Terminus of Alpha-Tropomyosin Encoded by Exon 9d ;

Method: SOLUTION NMR Dmax: 68.5 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 2k8x

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2k8x
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2k8x
沉积日期 deposition_date2008-09-25
结构标题 title;GlyTM1b(1-19)zip: A Chimeric Peptide Model of the N-Terminus of a Rat Short Alpha-Tropomyosin with the N-Terminus Encoded by Exon 1b in Complex with TM9d(252-284), a Peptide Model Containing the C Terminus of Alpha-Tropomyosin Encoded by Exon 9d ;
关键词 keywords;tropomyosin, N terminus, protein complex, C terminus, tropomodulin binding protein, overlap complex, coiled coil, cytoskeletal regulatory protein, ACTIN-BINDING PROTEIN ;; ACTIN-BINDING PROTEIN
实验方法 methodSOLUTION NMR

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier17.24
回转半径 Rg (电子) rg_electron16.99
零角强度 I(0) i0122598000.00
分子量 molecular_weight86519.0 kDa
排除体积 excluded_volume106840 ų
包络体积 envelope_volume28971 ų
水化壳体积 shell_volume13424 ų
包络直径 envelope_diameter71.2
壳层 Rg shell_rg24.44
包络 Rg envelope_rg20.73
形状 Rg shape_rg16.92
总 Rg total_rg17.52
总原子数 total_atoms12380
残基数 n_residues760
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax68.5
Rg (实空间) rg_real17.48
Rg 误差 (实空间) rg_real_error0.75
I(0) (实空间) i0_real1.2260e+08
I(0) 误差 (实空间) i0_real_error1.7290e+06
Rg (倒空间) rg_reciprocal17.45
I(0) (倒空间) i0_reciprocal122600000.0000
解质量估计 total_estimate0.7619
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary18.9
偏度 Skewness skewness0.576
峰度 Kurtosis kurtosis-0.021
角度范围 angular_range— – 0.4600 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha77140.0000
实空间数据点数 n_real_points77
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.516; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.353; Smooth: 0.999

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (1)

6. 折叠分类 (SCOP + CATH) 2 domains

SCOP 2.08 (2 domains)

结构域编号 domain_idd2k8xa_
类 Class classh — Coiled coil proteins
折叠类型 Fold foldh.1 — Parallel coiled-coil
超家族 Superfamily superfamilyh.1.3 — Leucine zipper domain
家族 Family familyh.1.3.1 — Leucine zipper domain
结构域编号 domain_idd2k8xb_
类 Class classh — Coiled coil proteins
折叠类型 Fold foldh.1 — Parallel coiled-coil
超家族 Superfamily superfamilyh.1.3 — Leucine zipper domain
家族 Family familyh.1.3.1 — Leucine zipper domain

7. 引用文献 (1)

8. 文件与曲线 (10)