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2DKY
Solution structure of the SAM-domain of Rho-GTPase-activating protein 7
Deposited 2006-04-14
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Different construct
Different experimental conditions
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Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
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Chain A
1–78（78 aa）
Fragment：SAM-domain
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Not recorded
|
No recorded non-water small molecule
|
SOLUTION NMR
NMR measurement conditions
pH 7；296 K；Ionic strength (raw mmCIF value) 120mM；Pressure AMBIENT
NMR sample composition
1.08mM SAM DOMAIN, 20mM d-TRIS-HCL, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O | 90% H2O/10% D2O
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Resolution not provided
|
|
2GYT
Solution structure of the SAM (sterile alpha motif) domain of DLC1 (deleted in liver cancer 1)
Deposited 2006-05-10
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Different construct
Different experimental conditions
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Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1–76（76 aa）
Fragment：sterile alpha motif (SAM) domain
|
Not recorded
|
No recorded non-water small molecule
|
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 50mM sodium phosphate；Pressure 1
NMR sample composition
1mM DLC1-SAM 15N, 13C-labaled; 50mM sodium phosphate buffer(pH 7.0);
3mM DTT; 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
1mM DLC1-SAM 15N-labaled; 50mM sodium phosphate buffer(pH 7.0);
3mM DTT; 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided
|
|
2LOZ
The novel binding mode of DLC1 and Tensin2 PTB domain
Deposited 2012-01-29
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Different construct
Different oligomeric state
Different experimental conditions
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Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
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Chain B
811–824（14 aa）
Fragment：UNP residues 811-824
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Not recorded
|
No recorded non-water small molecule
|
SOLUTION NMR
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 0.2；Pressure ambient
NMR sample composition
0.6-0.8 mM [U-100% 13C; U-100% 15N] Tensin2-1, 1.2-1.4 mM DLC1-2, 100 mM potassium phosphate-3, 100 mM potassium chloride-4, 1 mM EDTA-5, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.2-1.4 mM Tensin2-6, 0.6-0.8 mM [U-100% 13C; U-100% 15N] DLC1-7, 100 mM potassium phosphate-8, 100 mM potassium chloride-9, 1 mM EDTA-10, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.6-0.8 mM [U-100% 13C] Tensin2-11, 1.2-1.4 mM DLC1-12, 100 mM potassium phosphate-13, 100 mM potassium chloride-14, 1 mM EDTA-15, 100% D2O | 100% D2O
NMR sample composition
1.2-1.4 mM Tensin2-16, 0.6-0.8 mM [U-100% 13C] DLC1-17, 100 mM potassium phosphate-18, 100 mM potassium chloride-19, 1 mM EDTA-20, 100% D2O | 100% D2O
|
Resolution not provided
|
|
3KUQ
Crystal structure of the DLC1 RhoGAP domain
Deposited 2009-11-27
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Different construct
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1066–1275（210 aa）
Fragment：Rho-GAP domain
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Not recorded
|
UNX UNKNOWN LIGAND × 4
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X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；291 K；30% PEG-3350, 0.2M sodium chloride, 5% glycerol, 0.02M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 291K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å
R-free 0.260
|
|
5FZT
The crystal structure of R7R8 in complex with a DLC1 fragment.
Deposited 2016-03-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
467–489（23 aa）
Fragment：TBS, UNP RESIDUES 467-489
|
Not recorded
|
MLI MALONATE ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.4；15% ETHANOL, 0.1 M TRIS PH 7.4 AT 4OC
|
Resolution 2.10 Å
R-free 0.231
|
