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2kek

Solution structure of a dimer of LAC repressor DNA-binding domain complexed to its natural operator O3

Method: SOLUTION NMR
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Lactose operon repressor

Escherichia coli

UniProt P03023

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Homooligomer Protein 2 DNA 2 ;DNA (5'-D(*CP*GP*GP*CP*AP*GP*TP*GP*AP*GP*CP*GP*CP*AP*AP*CP*GP*CP*AP*AP*TP*TP*C)-3') ; × 1 ;DNA (5'-D(P*GP*AP*AP*TP*TP*GP*CP*GP*TP*TP*GP*CP*GP*CP*TP*CP*AP*CP*TP*GP*CP*CP*G)-3') ; × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer
1efa 1lqc

2 assemblies

Homodimer
1jwl 1jye 1jyf 2p9h 2paf

5 assemblies

Homotetramer
1lbh 1lbi 1tlf 3edc

4 assemblies

Other homooligomers

None

Heteromeric or nucleic-acid complex
1cjg 1efa 1jwl 1l1m 1lbg 1lbg 1lcc 1lcd 1osl 2bjc 2kei 2kej

15 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name LACI_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–62； UniProt 1–62 Author chain B； PDBConstruct 1–62； UniProt 1–62

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2kek
Deposition date deposition_date2009-01-30
Structure title titleSolution structure of a dimer of LAC repressor DNA-binding domain complexed to its natural operator O3
Keywords keywords;Lac repressor, lac operators, protein-DNA complex, DNA-binding, Repressor, Transcription, Transcription regulation, Transcription-DNA COMPLEX ;; Transcription/DNA
Experimental Method methodSOLUTION NMR
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2kek__assembly_1__model_2

Assembly 1 · Model 2 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2kek__assembly_1__model_2 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2kek__assembly_1__model_2 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）23.60 Å
Rg (electron density)23.14 Å
Total Rg23.70 Å
Atom count3374
Residues170
Excluded volume30605 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2kek__assembly_1__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
1 2 2kek__assembly_1__model_2 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
1 3 2kek__assembly_1__model_3 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
1 4 2kek__assembly_1__model_4 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
1 5 2kek__assembly_1__model_5 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
1 6 2kek__assembly_1__model_6 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
1 7 2kek__assembly_1__model_7 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
1 8 2kek__assembly_1__model_8 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
1 9 2kek__assembly_1__model_9 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
1 10 2kek__assembly_1__model_10 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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6. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd2keka_
Class classa — All alpha proteins
Fold Fold folda.35 — lambda repressor-like DNA-binding domains
Superfamily Superfamily superfamilya.35.1 — lambda repressor-like DNA-binding domains
Family Family familya.35.1.5 — GalR/LacI-like bacterial regulator
Domain ID domain_idd2kekb_
Class classa — All alpha proteins
Fold Fold folda.35 — lambda repressor-like DNA-binding domains
Superfamily Superfamily superfamilya.35.1 — lambda repressor-like DNA-binding domains
Family Family familya.35.1.5 — GalR/LacI-like bacterial regulator

CATH v4.4 (2 domains)

Domain ID domain_id2kekA00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology260 — 434 Repressor (Amino-terminal Domain)
Homologous superfamily homologous superfamily40 — lambda repressor-like DNA-binding domains
Domain ID domain_id2kekB00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology260 — 434 Repressor (Amino-terminal Domain)
Homologous superfamily homologous superfamily40 — lambda repressor-like DNA-binding domains
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7. Citations (1)