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2km8

Interdomain RRM packing contributes to RNA recognition in the rna15, hrp1, anchor RNA 3' processing ternary complex

Method: SOLUTION NMR
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1. Protein Identity and Related Structures Protein Identity & Related Structures

;mRNA 3'-end-processing protein RNA15 ;

Saccharomyces cerevisiae

UniProt P25299

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 2 RNA 1 5'-R(P*UP*AP*UP*AP*UP*AP*UP*AP*AP*UP*AP*AP*U)-3' × 1 Nuclear polyadenylated RNA-binding protein 4 × 1 （Q99383） Consistent with all polymers

Other States of the Same Protein in the Database

Monomer
2x1b

1 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2km8 2l9b 2x1a 2x1f

4 assemblies

View Construct and Data Evidence
UniProt name RNA15_YEAST
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–84； UniProt 14–97

Nuclear polyadenylated RNA-binding protein 4

Saccharomyces cerevisiae

UniProt Q99383

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 2 RNA 1 5'-R(P*UP*AP*UP*AP*UP*AP*UP*AP*AP*UP*AP*AP*U)-3' × 1 ;mRNA 3'-end-processing protein RNA15 ; × 1 （P25299） Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2cjk 2km8

2 assemblies

View Construct and Data Evidence
UniProt name HRP1_YEAST
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–167； UniProt 156–322

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2km8
Deposition date deposition_date2009-07-24
Structure title titleInterdomain RRM packing contributes to RNA recognition in the rna15, hrp1, anchor RNA 3' processing ternary complex
Keywords keywords;3' processing, RRM domain, rna15p, hrp1p, enhancer element, positioning element, RNA recognition, mRNA processing, Nucleus, RNA-binding, Nonsense-mediated mRNA decay, Phosphoprotein, RNA binding protein-RNA COMPLEX ;; RNA binding protein/RNA
Experimental Method methodSOLUTION NMR
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2km8__assembly_1__model_5

Assembly 1 · Model 5 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2km8__assembly_1__model_5 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2km8__assembly_1__model_5 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）21.14 Å
Rg (electron density)20.67 Å
Total Rg21.40 Å
Atom count4375
Residues264
Excluded volume39288 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2km8__assembly_1__model_1 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 2 2km8__assembly_1__model_2 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 3 2km8__assembly_1__model_3 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 4 2km8__assembly_1__model_4 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 5 2km8__assembly_1__model_5 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 6 2km8__assembly_1__model_6 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 7 2km8__assembly_1__model_7 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 8 2km8__assembly_1__model_8 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 9 2km8__assembly_1__model_9 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
1 10 2km8__assembly_1__model_10 trimeric （3） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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6. Fold Classification (SCOP + CATH) 6 domains

SCOP 2.08 (3 domains)

Domain ID domain_idd2km8b_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.7 — RNA-binding domain, RBD, aka RNA recognition motif (RRM)
Family Family familyd.58.7.0 — automated matches
Domain ID domain_idd2km8c1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.7 — RNA-binding domain, RBD, aka RNA recognition motif (RRM)
Family Family familyd.58.7.0 — automated matches
Domain ID domain_idd2km8c2
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.7 — RNA-binding domain, RBD, aka RNA recognition motif (RRM)
Family Family familyd.58.7.0 — automated matches

CATH v4.4 (3 domains)

Domain ID domain_id2km8B00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily330 — RRM (RNA recognition motif) domain
Domain ID domain_id2km8C01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily330 — RRM (RNA recognition motif) domain
Domain ID domain_id2km8C02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily330 — RRM (RNA recognition motif) domain
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7. Citations (1)