2mlx

NMR structure of E. coli Trigger Factor in complex with unfolded PhoA220-310

Method: SOLUTION NMR
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Trigger factor

Escherichia coli

UniProt U6N325

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Alkaline phosphatase × 1 (U6N3P1) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name U6N325_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 12–443; UniProt 1–432

Alkaline phosphatase

Escherichia coli

UniProt U6N3P1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Trigger factor × 1 (U6N325) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name U6N3P1_ECOLI
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B; PDBConstruct 3–93; UniProt 220–310

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id2mlx
Deposition date deposition_date2014-03-05
Structure title titleNMR structure of E. coli Trigger Factor in complex with unfolded PhoA220-310
Keywords keywordsmolecular chaperone, unfolded protein, protein complex, chaperone; CHAPERONE
Experimental Method methodSOLUTION NMR
▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2mlx__assembly_1__model_9

Assembly 1 · Model 9 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2mlx__assembly_1__model_9 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2mlx__assembly_1__model_9 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)33.44 Å
Rg (electron density)33.45 Å
Total Rg33.78 Å
Atom count8128
Residues523
Excluded volume72092 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2mlx__assembly_1__model_1 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 2 2mlx__assembly_1__model_2 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 3 2mlx__assembly_1__model_3 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 4 2mlx__assembly_1__model_4 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 5 2mlx__assembly_1__model_5 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 6 2mlx__assembly_1__model_6 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 7 2mlx__assembly_1__model_7 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 8 2mlx__assembly_1__model_8 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 9 2mlx__assembly_1__model_9 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 10 2mlx__assembly_1__model_10 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (2)

▼

6. Fold Classification (SCOP + CATH) 6 domains

SCOP 2.08 (3 domains)

Domain ID domain_idd2mlxa1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.241 — Ribosome binding domain-like
Superfamily Superfamily superfamilyd.241.2 — Trigger factor ribosome-binding domain
Family Family familyd.241.2.0 — automated matches
Domain ID domain_idd2mlxa2
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.26 — FKBP-like
Superfamily Superfamily superfamilyd.26.1 — FKBP-like
Family Family familyd.26.1.0 — automated matches
Domain ID domain_idd2mlxa3
Class classa — All alpha proteins
Fold Fold folda.223 — Triger factor/SurA peptide-binding domain-like
Superfamily Superfamily superfamilya.223.1 — Triger factor/SurA peptide-binding domain-like
Family Family familya.223.1.0 — automated matches

CATH v4.4 (3 domains)

Domain ID domain_id2mlxA01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily1050 — Trigger factor ribosome-binding domain
Domain ID domain_id2mlxA02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology3120 — Trigger factor, domain 2
Homologous superfamily homologous superfamily10 — Trigger factor, C-terminal domain
Domain ID domain_id2mlxA03
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology50 — Chitinase A; domain 3
Homologous superfamily homologous superfamily40 —
▶

7. Citations (1)