SAXSdb — 2oaa

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1. Protein Identity and Related Structures Protein Identity & Related Structures

R.MvaI

Kocuria varians

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–DNA	Monomer	Protein 1 DNA 2	5'-D(GPGPTPAPCPCPTPGPGPAPT)-3' × 1 5'-D(CPAPTPCPCPAPGPGPTPAPC)-3' × 1 CALCIUM ION × 3 water × 3	Consistent with all polymers
2	Protein–DNA	Monomer	Protein 1 DNA 2	5'-D(GPGPTPAPCPCPTPGPGPAPT)-3' × 1 5'-D(CPAPTPCPCPAPGPGPTPAPC)-3' × 1 CALCIUM ION × 4 water × 3	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q8RNV5_MICVA
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain A； PDBConstruct 4–249； UniProt 1–246 Author chain B； PDBConstruct 4–249； UniProt 1–246

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2oaa
Deposition date deposition_date	2006-12-15
Structure title title	Restriction endonuclease MvaI-cognate DNA substrate complex
Keywords keywords	MONOMERIC ENDONUCLEASE, DNA SUBSTRATE COMPLEX, RESTRICTION ENZYME, MVAI, HYDROLASE-DNA COMPLEX; HYDROLASE/DNA
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2oaa__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2oaa__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

2oaa__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	19.31 Å
Rg (electron density)	18.68 Å
Total Rg	19.51 Å
Atom count	2428
Residues	268
Excluded volume	41780 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2oaa__assembly_1__model_1`	trimeric （3）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`2oaa__assembly_2__model_1`	trimeric （3）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Description description	5'-D(GPGPTPAPCPCPTPGPGPAP*T)-3'
Molecular weight formula_weight	3389.2 kDa
Source method src_method	syn
Copy count entity_count	2

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	C,E
Atom count atom_count	5532

GGTACCTGGAT

Entity 2 Entity 2 polymer

Description description	5'-D(CPAPTPCPCPAPGPGPTPAP*C)-3'
Molecular weight formula_weight	3318.2 kDa
Source method src_method	syn
Copy count entity_count	2

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	D,F

CATCCAGGTAC

Entity 3 Entity 3 polymer

Description description	R.MvaI
Molecular weight formula_weight	28702.3 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B

MKSMSEYLNLLKEAIQNVVDGGWHETKRKGNTGIGKTFEDLLEKEEDNLDAPDFHDIEIKTHETAAKSLLTLFTKSPTNPRGANTMLRNRYGKKDEYGNNILHQTVSGNRKTNSNSYNYDFKIDIDWESQVVRLEVFDKQDIMIDNSVYWSFDSLQNQLDKKLKYIAVISAESKIENEKKYYKYNSANLFTDLTVQSLCRGIENGDIKVDIRIGAYHSGKKKGKTHDHGTAFRINMEKLLEYGEVKVIV

Source Source

Source organism organism	Kocuria varians
NCBI taxonomy ID ncbi_taxonomy_id	1272
Host organism host_organism	Escherichia coli

Entity 4 Entity 4 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	7

Entity 5 Entity 5 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	576

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6. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

Domain ID domain_id	2oaaA01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	210 — PvuII Endonuclease; Chain A
Homologous superfamily homologous superfamily	20 — MvaI/BcnI restriction endonuclease, catalytic domain

Domain ID domain_id	2oaaA02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	3570 — MvaI/BcnI restriction endonuclease, recognition domain

Domain ID domain_id	2oaaB01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	210 — PvuII Endonuclease; Chain A
Homologous superfamily homologous superfamily	20 — MvaI/BcnI restriction endonuclease, catalytic domain

Domain ID domain_id	2oaaB02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	3570 — MvaI/BcnI restriction endonuclease, recognition domain

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Unit-cell length a length_a	53.848 Å
Unit-cell length b length_b	81.380 Å
Unit-cell length c length_c	71.308 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.52 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	4

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	—

Resolution resolution	1.500 Å
Low-resolution limit resolution_low	30.000 Å
R factor r_factor	0.1770
R_work r_work	0.1760
R_free r_free	0.1940

Restriction endonuclease MvaI-cognate DNA substrate complex

1. Protein Identity and Related Structures Protein Identity & Related Structures

R.MvaI

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2oaa__assembly_1__model_1

2oaa__assembly_1__model_1 | I(q)

2oaa__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

7. Citations (2)

Restriction endonuclease MvaI-cognate DNA substrate complex

1. Protein Identity and Related Structures Protein Identity & Related Structures

R.MvaI

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2oaa__assembly_1__model_1

2oaa__assembly_1__model_1 | I(q)

2oaa__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 4 domains

CATH v4.4 (4 domains)

7. Citations (2)