2oar

Mechanosensitive Channel of Large Conductance (MscL)

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Large-conductance mechanosensitive channel

Mycobacterium tuberculosis H37Ra

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 5	GOLD ION × 3	Consistent with protein count
2	Protein homooligomer	Homooligomer	Protein 10	GOLD ION × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MSCL_MYCTU
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 24–174； UniProt 1–151 Author chain B； PDBConstruct 24–174； UniProt 1–151 Author chain C； PDBConstruct 24–174； UniProt 1–151 Author chain D； PDBConstruct 24–174； UniProt 1–151 Author chain E； PDBConstruct 24–174； UniProt 1–151

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2oar
Deposition date deposition_date	2006-12-17
Structure title title	Mechanosensitive Channel of Large Conductance (MscL)
Keywords keywords	stretch activated ion channel mechanosensitive, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2oar__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2oar__assembly_2__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

2oar__assembly_2__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	44.94 Å
Rg (electron density)	45.54 Å
Total Rg	45.15 Å
Atom count	9515
Residues	1250
Excluded volume	174370 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2oar__assembly_1__model_1`	pentameric （5）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`2oar__assembly_2__model_1`	decameric （10）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

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6. Fold Classification (SCOP + CATH) 13 domains

SCOP 2.08 (5 domains)

Domain ID domain_id	d2oara1
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.16 — Gated mechanosensitive channel
Superfamily Superfamily superfamily	f.16.1 — Gated mechanosensitive channel
Family Family family	f.16.1.1 — Gated mechanosensitive channel

Domain ID domain_id	d2oarb1
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.16 — Gated mechanosensitive channel
Superfamily Superfamily superfamily	f.16.1 — Gated mechanosensitive channel
Family Family family	f.16.1.1 — Gated mechanosensitive channel

Domain ID domain_id	d2oarc1
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.16 — Gated mechanosensitive channel
Superfamily Superfamily superfamily	f.16.1 — Gated mechanosensitive channel
Family Family family	f.16.1.1 — Gated mechanosensitive channel

Domain ID domain_id	d2oard1
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.16 — Gated mechanosensitive channel
Superfamily Superfamily superfamily	f.16.1 — Gated mechanosensitive channel
Family Family family	f.16.1.1 — Gated mechanosensitive channel

Domain ID domain_id	d2oare1
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.16 — Gated mechanosensitive channel
Superfamily Superfamily superfamily	f.16.1 — Gated mechanosensitive channel
Family Family family	f.16.1.1 — Gated mechanosensitive channel

CATH v4.4 (8 domains)

Domain ID domain_id	2oarA01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1200 — Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A
Homologous superfamily homologous superfamily	120 — Large-conductance mechanosensitive channel, MscL; domain 1

Domain ID domain_id	2oarA02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily	220 —

Domain ID domain_id	2oarB00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1200 — Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A
Homologous superfamily homologous superfamily	120 — Large-conductance mechanosensitive channel, MscL; domain 1

Domain ID domain_id	2oarC01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1200 — Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A
Homologous superfamily homologous superfamily	120 — Large-conductance mechanosensitive channel, MscL; domain 1

Domain ID domain_id	2oarC02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily	220 —

Domain ID domain_id	2oarD00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1200 — Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A
Homologous superfamily homologous superfamily	120 — Large-conductance mechanosensitive channel, MscL; domain 1

Domain ID domain_id	2oarE01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1200 — Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A
Homologous superfamily homologous superfamily	120 — Large-conductance mechanosensitive channel, MscL; domain 1

Domain ID domain_id	2oarE02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily	220 —

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Unit-cell length a length_a	212.308 Å
Unit-cell length b length_b	212.308 Å
Unit-cell length c length_c	97.680 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	40

Space group space_group	I 41
Space-group number int_tables_number	80
Crystal system crystal_system	—

Resolution resolution	3.500 Å
Low-resolution limit resolution_low	20.000 Å
R factor r_factor	0.3190
R_work r_work	0.3190
R_free r_free	0.3380

Description description	Large-conductance mechanosensitive channel
Molecular weight formula_weight	18817.3 kDa
Source method src_method	man
Copy count entity_count	5

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E
Atom count atom_count	4758

Mechanosensitive Channel of Large Conductance (MscL)

1. Protein Identity and Related Structures Protein Identity & Related Structures

Large-conductance mechanosensitive channel

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2oar__assembly_2__model_1

2oar__assembly_2__model_1 | I(q)

2oar__assembly_2__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

6. Fold Classification (SCOP + CATH) 13 domains

SCOP 2.08 (5 domains)

CATH v4.4 (8 domains)

7. Citations (3)

Source organism organism	Mycobacterium tuberculosis H37Ra
NCBI taxonomy ID ncbi_taxonomy_id	419947
Host organism host_organism	Escherichia coli BL21

Description description	GOLD ION
Molecular weight formula_weight	197.0 kDa
Source method src_method	syn
Copy count entity_count	3

Mechanosensitive Channel of Large Conductance (MscL)

1. Protein Identity and Related Structures Protein Identity & Related Structures

Large-conductance mechanosensitive channel

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2oar__assembly_2__model_1

2oar__assembly_2__model_1 | I(q)

2oar__assembly_2__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

6. Fold Classification (SCOP + CATH) 13 domains

SCOP 2.08 (5 domains)

CATH v4.4 (8 domains)

7. Citations (3)