2oau

Mechanosensitive Channel of Small Conductance (MscS)

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Small-conductance mechanosensitive channel

Escherichia coli

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 7	No other associated polymer	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MSCS_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 21–306； UniProt 1–286 Author chain B； PDBConstruct 21–306； UniProt 1–286 Author chain C； PDBConstruct 21–306； UniProt 1–286 Author chain D； PDBConstruct 21–306； UniProt 1–286 Author chain E； PDBConstruct 21–306； UniProt 1–286 Author chain F； PDBConstruct 21–306； UniProt 1–286 Author chain G； PDBConstruct 21–306； UniProt 1–286

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2oau
Deposition date deposition_date	2006-12-17
Structure title title	Mechanosensitive Channel of Small Conductance (MscS)
Keywords keywords	stretch activated, mechanosensitive ion channel, membrane protein; MEMBRANE PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2oau__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2oau__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

2oau__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	40.64 Å
Rg (electron density)	40.19 Å
Total Rg	40.69 Å
Atom count	13573
Residues	1778
Excluded volume	245960 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2oau__assembly_1__model_1`	heptameric （7）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (1)

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6. Fold Classification (SCOP + CATH) 42 domains

SCOP 2.08 (21 domains)

Domain ID domain_id	d2oaua1
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.3 — Mechanosensitive channel protein MscS (YggB), middle domain

Domain ID domain_id	d2oaua2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.43 — Mechanosensitive channel protein MscS (YggB), C-terminal domain
Family Family family	d.58.43.1 — Mechanosensitive channel protein MscS (YggB), C-terminal domain

Domain ID domain_id	d2oaua3
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.34 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Superfamily Superfamily superfamily	f.34.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Family Family family	f.34.1.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region

Domain ID domain_id	d2oaub1
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.3 — Mechanosensitive channel protein MscS (YggB), middle domain

Domain ID domain_id	d2oaub2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.43 — Mechanosensitive channel protein MscS (YggB), C-terminal domain
Family Family family	d.58.43.1 — Mechanosensitive channel protein MscS (YggB), C-terminal domain

Domain ID domain_id	d2oaub3
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.34 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Superfamily Superfamily superfamily	f.34.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Family Family family	f.34.1.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region

Domain ID domain_id	d2oauc1
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.3 — Mechanosensitive channel protein MscS (YggB), middle domain

Domain ID domain_id	d2oauc2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.43 — Mechanosensitive channel protein MscS (YggB), C-terminal domain
Family Family family	d.58.43.1 — Mechanosensitive channel protein MscS (YggB), C-terminal domain

Domain ID domain_id	d2oauc3
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.34 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Superfamily Superfamily superfamily	f.34.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Family Family family	f.34.1.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region

Domain ID domain_id	d2oaud1
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.3 — Mechanosensitive channel protein MscS (YggB), middle domain

Domain ID domain_id	d2oaud2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.43 — Mechanosensitive channel protein MscS (YggB), C-terminal domain
Family Family family	d.58.43.1 — Mechanosensitive channel protein MscS (YggB), C-terminal domain

Domain ID domain_id	d2oaud3
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.34 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Superfamily Superfamily superfamily	f.34.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Family Family family	f.34.1.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region

Domain ID domain_id	d2oaue1
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.3 — Mechanosensitive channel protein MscS (YggB), middle domain

Domain ID domain_id	d2oaue2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.43 — Mechanosensitive channel protein MscS (YggB), C-terminal domain
Family Family family	d.58.43.1 — Mechanosensitive channel protein MscS (YggB), C-terminal domain

Domain ID domain_id	d2oaue3
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.34 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Superfamily Superfamily superfamily	f.34.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Family Family family	f.34.1.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region

Domain ID domain_id	d2oauf1
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.3 — Mechanosensitive channel protein MscS (YggB), middle domain

Domain ID domain_id	d2oauf2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.43 — Mechanosensitive channel protein MscS (YggB), C-terminal domain
Family Family family	d.58.43.1 — Mechanosensitive channel protein MscS (YggB), C-terminal domain

Domain ID domain_id	d2oauf3
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.34 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Superfamily Superfamily superfamily	f.34.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Family Family family	f.34.1.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region

Domain ID domain_id	d2oaug1
Class class	b — All beta proteins
Fold Fold fold	b.38 — Sm-like fold
Superfamily Superfamily superfamily	b.38.1 — Sm-like ribonucleoproteins
Family Family family	b.38.1.3 — Mechanosensitive channel protein MscS (YggB), middle domain

Domain ID domain_id	d2oaug2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.58 — Ferredoxin-like
Superfamily Superfamily superfamily	d.58.43 — Mechanosensitive channel protein MscS (YggB), C-terminal domain
Family Family family	d.58.43.1 — Mechanosensitive channel protein MscS (YggB), C-terminal domain

Domain ID domain_id	d2oaug3
Class class	f — Membrane and cell surface proteins and peptides
Fold Fold fold	f.34 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Superfamily Superfamily superfamily	f.34.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region
Family Family family	f.34.1.1 — Mechanosensitive channel protein MscS (YggB), transmembrane region

CATH v4.4 (21 domains)

Domain ID domain_id	2oauA01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	1260 —

Domain ID domain_id	2oauA02
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	60 —

Domain ID domain_id	2oauA03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	2oauB01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	1260 —

Domain ID domain_id	2oauB02
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	60 —

Domain ID domain_id	2oauB03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	2oauC01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	1260 —

Domain ID domain_id	2oauC02
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	60 —

Domain ID domain_id	2oauC03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	2oauD01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	1260 —

Domain ID domain_id	2oauD02
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	60 —

Domain ID domain_id	2oauD03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	2oauE01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	1260 —

Domain ID domain_id	2oauE02
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	60 —

Domain ID domain_id	2oauE03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	2oauF01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	1260 —

Domain ID domain_id	2oauF02
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	60 —

Domain ID domain_id	2oauF03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	2oauG01
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	287 — Helix Hairpins
Homologous superfamily homologous superfamily	1260 —

Domain ID domain_id	2oauG02
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	60 —

Domain ID domain_id	2oauG03
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	100 —

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Unit-cell length a length_a	184.274 Å
Unit-cell length b length_b	184.274 Å
Unit-cell length c length_c	260.485 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	56

Space group space_group	P 43 21 2
Space-group number int_tables_number	96
Crystal system crystal_system	—

Resolution resolution	3.700 Å
Low-resolution limit resolution_low	50.000 Å
R factor r_factor	—
R_work r_work	0.2930
R_free r_free	0.3210

Description description	Small-conductance mechanosensitive channel
Molecular weight formula_weight	33094.3 kDa
Source method src_method	man
Copy count entity_count	7

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E,F,G
Atom count atom_count	13573

Mechanosensitive Channel of Small Conductance (MscS)

1. Protein Identity and Related Structures Protein Identity & Related Structures

Small-conductance mechanosensitive channel

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2oau__assembly_1__model_1

2oau__assembly_1__model_1 | I(q)

2oau__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

6. Fold Classification (SCOP + CATH) 42 domains

SCOP 2.08 (21 domains)

CATH v4.4 (21 domains)

7. Citations (2)

Source organism organism	Escherichia coli
NCBI taxonomy ID ncbi_taxonomy_id	562
Host organism host_organism	Escherichia coli BL21(DE3)

Mechanosensitive Channel of Small Conductance (MscS)

1. Protein Identity and Related Structures Protein Identity & Related Structures

Small-conductance mechanosensitive channel

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2oau__assembly_1__model_1

2oau__assembly_1__model_1 | I(q)

2oau__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

6. Fold Classification (SCOP + CATH) 42 domains

SCOP 2.08 (21 domains)

CATH v4.4 (21 domains)

7. Citations (2)