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2ov2

The crystal structure of the human RAC3 in complex with the CRIB domain of human p21-activated kinase 4 (PAK4)

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Ras-related C3 botulinum toxin substrate 3

Homo sapiens

UniProt P60763

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Serine/threonine-protein kinase PAK 4 × 1 （O96013） MAGNESIUM ION × 1 CHLORIDE ION × 2 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 water × 2 Consistent with protein count
10 Protein heterocomplex Heteromer Protein 4 Serine/threonine-protein kinase PAK 4 × 2 （O96013） MAGNESIUM ION × 2 CHLORIDE ION × 3 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 water × 4 Consistent with protein count
11 Protein heterocomplex Heteromer Protein 4 Serine/threonine-protein kinase PAK 4 × 2 （O96013） MAGNESIUM ION × 2 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 1,2-ETHANEDIOL × 2 water × 4 Consistent with protein count
12 Protein heterocomplex Heteromer Protein 4 Serine/threonine-protein kinase PAK 4 × 2 （O96013） MAGNESIUM ION × 2 CHLORIDE ION × 2 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 1,2-ETHANEDIOL × 1 water × 4 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 Serine/threonine-protein kinase PAK 4 × 1 （O96013） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 Serine/threonine-protein kinase PAK 4 × 1 （O96013） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 1,2-ETHANEDIOL × 1 water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 2 Serine/threonine-protein kinase PAK 4 × 1 （O96013） MAGNESIUM ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 1,2-ETHANEDIOL × 1 water × 2 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 2 Serine/threonine-protein kinase PAK 4 × 1 （O96013） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 water × 2 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 2 Serine/threonine-protein kinase PAK 4 × 1 （O96013） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 1,2-ETHANEDIOL × 1 water × 2 Consistent with protein count
7 Protein heterocomplex Heteromer Protein 2 Serine/threonine-protein kinase PAK 4 × 1 （O96013） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 water × 2 Consistent with protein count
8 Protein heterocomplex Heteromer Protein 2 Serine/threonine-protein kinase PAK 4 × 1 （O96013） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 water × 2 Consistent with protein count
9 Protein heterocomplex Heteromer Protein 4 Serine/threonine-protein kinase PAK 4 × 2 （O96013） MAGNESIUM ION × 2 CHLORIDE ION × 2 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
2c2h 2c2h 2g0n 2g0n 2ic5 2ic5

6 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2

15 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RAC3_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–178； UniProt 1–177 Author chain B； PDBConstruct 2–178； UniProt 1–177 Author chain C； PDBConstruct 2–178； UniProt 1–177 Author chain D； PDBConstruct 2–178； UniProt 1–177 Author chain E； PDBConstruct 2–178； UniProt 1–177 Author chain F； PDBConstruct 2–178； UniProt 1–177 Author chain G； PDBConstruct 2–178； UniProt 1–177 Author chain H； PDBConstruct 2–178； UniProt 1–177

Serine/threonine-protein kinase PAK 4

Homo sapiens

UniProt O96013

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Ras-related C3 botulinum toxin substrate 3 × 1 （P60763） MAGNESIUM ION × 1 CHLORIDE ION × 2 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 water × 2 Consistent with protein count
10 Protein heterocomplex Heteromer Protein 4 Ras-related C3 botulinum toxin substrate 3 × 2 （P60763） MAGNESIUM ION × 2 CHLORIDE ION × 3 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 water × 4 Consistent with protein count
11 Protein heterocomplex Heteromer Protein 4 Ras-related C3 botulinum toxin substrate 3 × 2 （P60763） MAGNESIUM ION × 2 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 1,2-ETHANEDIOL × 2 water × 4 Consistent with protein count
12 Protein heterocomplex Heteromer Protein 4 Ras-related C3 botulinum toxin substrate 3 × 2 （P60763） MAGNESIUM ION × 2 CHLORIDE ION × 2 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 1,2-ETHANEDIOL × 1 water × 4 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 Ras-related C3 botulinum toxin substrate 3 × 1 （P60763） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 Ras-related C3 botulinum toxin substrate 3 × 1 （P60763） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 1,2-ETHANEDIOL × 1 water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 2 Ras-related C3 botulinum toxin substrate 3 × 1 （P60763） MAGNESIUM ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 1,2-ETHANEDIOL × 1 water × 2 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 2 Ras-related C3 botulinum toxin substrate 3 × 1 （P60763） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 water × 2 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 2 Ras-related C3 botulinum toxin substrate 3 × 1 （P60763） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 1,2-ETHANEDIOL × 1 water × 2 Consistent with protein count
7 Protein heterocomplex Heteromer Protein 2 Ras-related C3 botulinum toxin substrate 3 × 1 （P60763） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 water × 2 Consistent with protein count
8 Protein heterocomplex Heteromer Protein 2 Ras-related C3 botulinum toxin substrate 3 × 1 （P60763） MAGNESIUM ION × 1 CHLORIDE ION × 1 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 water × 2 Consistent with protein count
9 Protein heterocomplex Heteromer Protein 4 Ras-related C3 botulinum toxin substrate 3 × 2 （P60763） MAGNESIUM ION × 2 CHLORIDE ION × 2 PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
2cdz 2j0i 2x4z 4app 4fie 4fie 4fig 4fig 4fih 4fij 4l67 4l67

34 assemblies; showing the first 12

Homodimer
4fif 4fif 4fii

3 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2 2ov2

25 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name PAK4_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain I； PDBConstruct 1–35； UniProt 10–44 Author chain J； PDBConstruct 1–35； UniProt 10–44 Author chain K； PDBConstruct 1–35； UniProt 10–44 Author chain L； PDBConstruct 1–35； UniProt 10–44 Author chain M； PDBConstruct 1–35； UniProt 10–44 Author chain N； PDBConstruct 1–35； UniProt 10–44 Author chain O； PDBConstruct 1–35； UniProt 10–44 Author chain P； PDBConstruct 1–35； UniProt 10–44

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2ov2
Deposition date deposition_date2007-02-12
Structure title titleThe crystal structure of the human RAC3 in complex with the CRIB domain of human p21-activated kinase 4 (PAK4)
Keywords keywords;GTPase RAC3, small GTP binding protein, p21 rac, ras-related C3 botulinum toxin substrate 3, signalling protein, CRIB, kinase, Structural Genomics, Structural Genomics Consortium, SGC, PROTEIN BINDING-TRANSFERASE COMPLEX ;; PROTEIN BINDING/TRANSFERASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2ov2__assembly_4__model_1

Assembly 4 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2ov2__assembly_4__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2ov2__assembly_4__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）18.10 Å
Rg (electron density)16.93 Å
Total Rg17.90 Å
Atom count1666
Residues214
Excluded volume29611 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2ov2__assembly_1__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 2ov2__assembly_2__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 2ov2__assembly_3__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 2ov2__assembly_4__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 2ov2__assembly_5__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 2ov2__assembly_6__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
7 1 2ov2__assembly_7__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
8 1 2ov2__assembly_8__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
9 1 2ov2__assembly_9__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
10 1 2ov2__assembly_10__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
11 1 2ov2__assembly_11__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
12 1 2ov2__assembly_12__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

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6. Fold Classification (SCOP + CATH) 27 domains

SCOP 2.08 (19 domains)

Domain ID domain_idd2ov2a1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd2ov2a2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd2ov2b1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd2ov2b2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd2ov2c1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd2ov2c2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd2ov2d1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd2ov2d2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd2ov2d3
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd2ov2e1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd2ov2e2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd2ov2f1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd2ov2f2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd2ov2f3
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd2ov2g1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd2ov2g2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd2ov2h1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family familyc.37.1.8 — G proteins
Domain ID domain_idd2ov2h2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd2ov2h3
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags

CATH v4.4 (8 domains)

Domain ID domain_id2ov2A00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id2ov2B00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id2ov2C00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id2ov2D00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id2ov2E00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id2ov2F00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id2ov2G00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id2ov2H00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
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7. Citations (1)