2p5g

Crystal structure of RB69 gp43 in complex with DNA with dAMP opposite an abasic site analog in a 21mer template

Method: X-RAY DIFFRACTION Dmax: 177.4 Å Quality: GOOD

1. 蛋白身份与相关结构 Protein Identity & Related Structures

DNA polymerase

Enterobacteria phage RB69

UniProt Q38087

当前结构中的状态

Assembly 聚集状态 构建体 突变与修饰 配体、离子与共同组分 实验方法与环境 结构质量
1 蛋白–DNA 单体 蛋白 × 1 DNA 2 PDB 声明:trimeric(3) 与全部聚合物数一致 链 A; UniProt 1–903 突变:D222A,D327A 非标准单体:是(mmCIF未提供具体位点) Template DNA × 1 Primer DNA × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, HANGING DROP;pH 7;293 K;PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K 分辨率 2.80 Å R-free 0.295
2 蛋白–DNA 单体 蛋白 × 1 DNA 2 PDB 声明:trimeric(3) 与全部聚合物数一致 链 B; UniProt 1–903 突变:D222A,D327A 非标准单体:是(mmCIF未提供具体位点) Template DNA × 1 Primer DNA × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, HANGING DROP;pH 7;293 K;PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K 分辨率 2.80 Å R-free 0.295
3 蛋白–DNA 单体 蛋白 × 1 DNA 2 PDB 声明:trimeric(3) 与全部聚合物数一致 链 C; UniProt 1–903 突变:D222A,D327A 非标准单体:是(mmCIF未提供具体位点) Template DNA × 1 Primer DNA × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, HANGING DROP;pH 7;293 K;PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K 分辨率 2.80 Å R-free 0.295
4 蛋白–DNA 单体 蛋白 × 1 DNA 2 PDB 声明:trimeric(3) 与全部聚合物数一致 链 D; UniProt 1–903 突变:D222A,D327A 非标准单体:是(mmCIF未提供具体位点) Template DNA × 1 Primer DNA × 1 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, HANGING DROP;pH 7;293 K;PEG 2000 MME, sodium acetate, magnesium sulfate, beta-mercaptoethanol, HEPES 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K 分辨率 2.80 Å R-free 0.295

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly, “相对当前条目”直接指出证据层面的不同;没有差异标签表示当前已读取字段一致。

共 120 个其他 PDB 条目、163 个 assembly。 打开独立比较页并筛选聚集状态

查看构建体与数据证据
UniProt名称 DPOL_BPR69
Isoform
PDB实体 3
链与序列区间 作者链 A; PDB构建体 1–903; UniProt 1–903 作者链 B; PDB构建体 1–903; UniProt 1–903 作者链 C; PDB构建体 1–903; UniProt 1–903 作者链 D; PDB构建体 1–903; UniProt 1–903

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

SAXS profile for 2p5g

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2p5g
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2. 结构基本信息 2. Structure Basics

条目编号 entry_id2p5g
沉积日期 deposition_date2007-03-15
结构标题 titleCrystal structure of RB69 gp43 in complex with DNA with dAMP opposite an abasic site analog in a 21mer template
关键词 keywordsDNA POLYMERASE, ABASIC SITE, DNA LESION, dAMP, transferase-DNA COMPLEX; transferase/DNA
实验方法 methodX-RAY DIFFRACTION

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier60.10
回转半径 Rg (电子) rg_electron59.56
零角强度 I(0) i02775460000.00
分子量 molecular_weight413600.0 kDa
排除体积 excluded_volume502710 ų
包络体积 envelope_volume818830 ų
水化壳体积 shell_volume113480 ų
包络直径 envelope_diameter193.8
壳层 Rg shell_rg63.18
包络 Rg envelope_rg57.40
形状 Rg shape_rg59.62
总 Rg total_rg59.46
总原子数 total_atoms28776
残基数 n_residues3426
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax177.4
Rg (实空间) rg_real59.76
Rg 误差 (实空间) rg_real_error1.13
I(0) (实空间) i0_real2.7750e+09
I(0) 误差 (实空间) i0_real_error5.3030e+07
Rg (倒空间) rg_reciprocal60.36
I(0) (倒空间) i0_reciprocal2778000000.0000
解质量估计 total_estimate0.8504
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary88.7
偏度 Skewness skewness0.010
峰度 Kurtosis kurtosis-0.686
角度范围 angular_range— – 0.1300 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha179300000.0000
实空间数据点数 n_real_points27
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.982; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.995; Smooth: 0.109

5. 晶体学与实验 5. Crystallography & Experiment

6. 实体与聚合物信息 Entities & Polymers (4)

7. 折叠分类 (SCOP + CATH) 30 domains

SCOP 2.08 (8 domains)

结构域编号 domain_idd2p5ga1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.3 — Ribonuclease H-like
家族 Family familyc.55.3.5 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_idd2p5ga2
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.8 — DNA/RNA polymerases
超家族 Superfamily superfamilye.8.1 — DNA/RNA polymerases
家族 Family familye.8.1.1 — DNA polymerase I
结构域编号 domain_idd2p5gb1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.3 — Ribonuclease H-like
家族 Family familyc.55.3.5 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_idd2p5gb2
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.8 — DNA/RNA polymerases
超家族 Superfamily superfamilye.8.1 — DNA/RNA polymerases
家族 Family familye.8.1.1 — DNA polymerase I
结构域编号 domain_idd2p5gc1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.3 — Ribonuclease H-like
家族 Family familyc.55.3.5 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_idd2p5gc2
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.8 — DNA/RNA polymerases
超家族 Superfamily superfamilye.8.1 — DNA/RNA polymerases
家族 Family familye.8.1.1 — DNA polymerase I
结构域编号 domain_idd2p5gd1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.3 — Ribonuclease H-like
家族 Family familyc.55.3.5 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_idd2p5gd2
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.8 — DNA/RNA polymerases
超家族 Superfamily superfamilye.8.1 — DNA/RNA polymerases
家族 Family familye.8.1.1 — DNA polymerase I

CATH v4.4 (22 domains)

结构域编号 domain_id2p5gA01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology342 — DNA Polymerase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — DNA Polymerase, chain B, domain 1
结构域编号 domain_id2p5gA02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease H-like superfamily/Ribonuclease H
结构域编号 domain_id2p5gA03
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1600 — Palm domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — B family DNA polymerase, palm domain
结构域编号 domain_id2p5gA04
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily690 — B family DNA polymerase, finger domain
结构域编号 domain_id2p5gA05
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1280 — Monooxygenase
同源超家族 H-superfamily homologous superfamily300
结构域编号 domain_id2p5gA06
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology1820 — Ribonuclease H-like motif
同源超家族 H-superfamily homologous superfamily10 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_id2p5gB01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology342 — DNA Polymerase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — DNA Polymerase, chain B, domain 1
结构域编号 domain_id2p5gB02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease H-like superfamily/Ribonuclease H
结构域编号 domain_id2p5gB03
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1600 — Palm domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — B family DNA polymerase, palm domain
结构域编号 domain_id2p5gB04
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1280 — Monooxygenase
同源超家族 H-superfamily homologous superfamily300
结构域编号 domain_id2p5gC01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology342 — DNA Polymerase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — DNA Polymerase, chain B, domain 1
结构域编号 domain_id2p5gC02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease H-like superfamily/Ribonuclease H
结构域编号 domain_id2p5gC03
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1600 — Palm domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — B family DNA polymerase, palm domain
结构域编号 domain_id2p5gC04
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily690 — B family DNA polymerase, finger domain
结构域编号 domain_id2p5gC05
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1280 — Monooxygenase
同源超家族 H-superfamily homologous superfamily300
结构域编号 domain_id2p5gC06
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology1820 — Ribonuclease H-like motif
同源超家族 H-superfamily homologous superfamily10 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_id2p5gD01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology342 — DNA Polymerase; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — DNA Polymerase, chain B, domain 1
结构域编号 domain_id2p5gD02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease H-like superfamily/Ribonuclease H
结构域编号 domain_id2p5gD03
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1600 — Palm domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — B family DNA polymerase, palm domain
结构域编号 domain_id2p5gD04
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily690 — B family DNA polymerase, finger domain
结构域编号 domain_id2p5gD05
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1280 — Monooxygenase
同源超家族 H-superfamily homologous superfamily300
结构域编号 domain_id2p5gD06
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology1820 — Ribonuclease H-like motif
同源超家族 H-superfamily homologous superfamily10 — DnaQ-like 3'-5' exonuclease

8. 引用文献 (1)

9. 文件与曲线 (10)