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2rf8

Crystal Structure of the mutant C2A conjugated bile acid hydrolase from Clostridium perfringens

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Choloylglycine hydrolase

Clostridium perfringens

UniProt P54965

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein homooligomer Homooligomer Protein 4 GLYCEROL × 6 water × 4 Consistent with protein count
2 Protein homooligomer Homooligomer Protein 4 GLYCEROL × 6 water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer
2bjf 2bjg 2rf8 2rf8 2rg2 2rlc

6 assemblies

Other homooligomers

None

Heteromeric or nucleic-acid complex

None

View Construct and Data Evidence
UniProt name CBH_CLOPE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–329； UniProt 1–329 Author chain B； PDBConstruct 1–329； UniProt 1–329

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2rf8
Deposition date deposition_date2007-09-28
Structure title titleCrystal Structure of the mutant C2A conjugated bile acid hydrolase from Clostridium perfringens
Keywords keywordsCholoylglycine hydrolase, bile salt hydrolase, CBAH, BSH, Ntn-hydrolase, HYDROLASE; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2rf8__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2rf8__assembly_2__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2rf8__assembly_2__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）62.02 Å
Rg (electron density)63.71 Å
Total Rg63.47 Å
Atom count10480
Residues1314
Excluded volume186930 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2rf8__assembly_1__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 2rf8__assembly_2__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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6. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd2rf8a_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.153 — Ntn hydrolase-like
Superfamily Superfamily superfamilyd.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family familyd.153.1.0 — automated matches
Domain ID domain_idd2rf8b_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.153 — Ntn hydrolase-like
Superfamily Superfamily superfamilyd.153.1 — N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Family Family familyd.153.1.0 — automated matches

CATH v4.4 (2 domains)

Domain ID domain_id2rf8A01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology60 — Penicillin V Acylase; Chain A
Homologous superfamily homologous superfamily10 — Penicillin V Acylase; Chain A
Domain ID domain_id2rf8B01
Class class3 — Alpha Beta
Architecture architecture60 — 4-Layer Sandwich
Topology topology60 — Penicillin V Acylase; Chain A
Homologous superfamily homologous superfamily10 — Penicillin V Acylase; Chain A
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7. Citations (1)