SAXSdb — 2uwj

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

TYPE III EXPORT PROTEIN PSCE

PSEUDOMONAS AERUGINOSA

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 6	TYPE III EXPORT PROTEIN PSCF × 2 （P95434） TYPE III EXPORT PROTEIN PSCG × 2 （P95435） NICKEL (II) ION × 6 water × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSCE_PSEAE
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain E； PDBConstruct 4–70； UniProt 1–67

TYPE III EXPORT PROTEIN PSCF

PSEUDOMONAS AERUGINOSA

UniProt P95434

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 6	TYPE III EXPORT PROTEIN PSCE × 2 （Q9I317） TYPE III EXPORT PROTEIN PSCG × 2 （P95435） NICKEL (II) ION × 6 water × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSCF_PSEAE
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain F； PDBConstruct 2–32； UniProt 54–84

TYPE III EXPORT PROTEIN PSCG

PSEUDOMONAS AERUGINOSA

UniProt P95435

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 6	TYPE III EXPORT PROTEIN PSCE × 2 （Q9I317） TYPE III EXPORT PROTEIN PSCF × 2 （P95434） NICKEL (II) ION × 6 water × 6	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSCG_PSEAE
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain G； PDBConstruct 1–115； UniProt 1–115

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	2uwj
Deposition date deposition_date	2007-03-22
Structure title title	Structure of the heterotrimeric complex which regulates type III secretion needle formation
Keywords keywords	VIRULENCE, CHAPERONES, COILED COIL, NEEDLE FORMATION, TYPE III SECRETION, BACTERIAL PATHOGENICITY, CHAPERONE; CHAPERONE
Experimental Method method	X-RAY DIFFRACTION

▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2uwj__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2uwj__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

2uwj__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	24.11 Å
Rg (electron density)	22.96 Å
Total Rg	23.85 Å
Atom count	3300
Residues	432
Excluded volume	58289 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2uwj__assembly_1__model_1`	hexameric （6）	Success	4.1.3-1-20251215 (887e7ef)	View Download

▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Description description	TYPE III EXPORT PROTEIN PSCE
Molecular weight formula_weight	7631.7 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E
Atom count atom_count	1822

GSHMMTALETRLSVADGTHAAALRQRLQAALAECRRELARGACPERFQFLQQQARALEGGLGILSQLTED

Source Source

Source organism organism	PSEUDOMONAS AERUGINOSA
NCBI taxonomy ID ncbi_taxonomy_id	1227568
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 2 Entity 2 polymer

Description description	TYPE III EXPORT PROTEIN PSCF
Molecular weight formula_weight	3791.5 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MHKINKWSVIYNINSTVTRALRDLMQGILQKI

Source Source

Source organism organism	PSEUDOMONAS AERUGINOSA
NCBI taxonomy ID ncbi_taxonomy_id	1227568
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 3 Entity 3 polymer

Description description	TYPE III EXPORT PROTEIN PSCG
Molecular weight formula_weight	12464.1 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MDTSLIRELAELALAGSGQHCHEEALCIAEWLERLGQDEAARLIRISSLANQGRYQEALAFAHGNPWPALEPWFALCEWHLGLGAALDRRLAGLGGSSDPALADFAAGMRAQVRT

Source Source

Source organism organism	PSEUDOMONAS AERUGINOSA
NCBI taxonomy ID ncbi_taxonomy_id	1227568
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 4 Entity 4 non-polymer

Description description	NICKEL (II) ION
Molecular weight formula_weight	58.7 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 5 Entity 5 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	172

▼

6. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

Domain ID domain_id	2uwjE00
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily	420 — Immunoglobulin FC, subunit C

Domain ID domain_id	2uwjG00
Class class	1 — Mainly Alpha
Architecture architecture	25 — Alpha Horseshoe
Topology topology	40 — Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Homologous superfamily homologous superfamily	10 — Tetratricopeptide repeat domain

▶

Unit-cell length a length_a	85.305 Å
Unit-cell length b length_b	85.305 Å
Unit-cell length c length_c	157.553 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	120.00 °
Molecules per unit cell Z z_pdb	12

Space group space_group	P 62 2 2
Space-group number int_tables_number	180
Crystal system crystal_system	—

Resolution resolution	2.000 Å
Low-resolution limit resolution_low	28.990 Å
R factor r_factor	0.2080
R_work r_work	0.2080
R_free r_free	0.2360

Structure of the heterotrimeric complex which regulates type III secretion needle formation

1. Protein Identity and Related Structures Protein Identity & Related Structures

TYPE III EXPORT PROTEIN PSCE

State in the Current Structure

Other States of the Same Protein in the Database

TYPE III EXPORT PROTEIN PSCF

State in the Current Structure

Other States of the Same Protein in the Database

TYPE III EXPORT PROTEIN PSCG

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2uwj__assembly_1__model_1

2uwj__assembly_1__model_1 | I(q)

2uwj__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

7. Citations (2)

Structure of the heterotrimeric complex which regulates type III secretion needle formation

1. Protein Identity and Related Structures Protein Identity & Related Structures

TYPE III EXPORT PROTEIN PSCE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

TYPE III EXPORT PROTEIN PSCF

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

TYPE III EXPORT PROTEIN PSCG

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2uwj__assembly_1__model_1

2uwj__assembly_1__model_1 | I(q)

2uwj__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 polymer

Polymer Polymer

Source Source

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

7. Citations (2)