SAXSdb — 2v7g

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1. Protein Identity and Related Structures Protein Identity & Related Structures

UROCANATE HYDRATASE

PSEUDOMONAS PUTIDA

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 4	ACETATE ION × 7 GLYCEROL × 9 NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 4 water × 4	Consistent with protein count
2	Protein homooligomer	Homooligomer	Protein 4	ACETATE ION × 7 GLYCEROL × 9 NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 4 water × 4	Consistent with protein count
3	Protein homooligomer	Homooligomer	Protein 2	ACETATE ION × 4 GLYCEROL × 2 NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 2 water × 2	Consistent with protein count
4	Protein homooligomer	Homooligomer	Protein 2	ACETATE ION × 3 GLYCEROL × 7 NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	HUTU_PSEPU
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 2–557； UniProt 1–556 Author chain B； PDBConstruct 2–557； UniProt 1–556 Author chain C； PDBConstruct 2–557； UniProt 1–556 Author chain D； PDBConstruct 2–557； UniProt 1–556

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2v7g
Deposition date deposition_date	2007-07-30
Structure title title	Crystal Structure of an Engineered Urocanase Tetramer
Keywords keywords	HISTIDINE DEGRADATION, NAD, LYASE, PROTEIN ENGINEERING, UROCANATE HYDRATASE, HISTIDINE METABOLISM; LYASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2v7g__assembly_3__model_1

Assembly 3 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2v7g__assembly_3__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

2v7g__assembly_3__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	31.17 Å
Rg (electron density)	30.65 Å
Total Rg	31.28 Å
Atom count	8596
Residues	1106
Excluded volume	151570 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2v7g__assembly_1__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`2v7g__assembly_2__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`2v7g__assembly_3__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
4	1	`2v7g__assembly_4__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Description description	UROCANATE HYDRATASE
Molecular weight formula_weight	60811.7 kDa
EC 编号 ec_number	4.2.1.49
Mutation mutation	YES
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D
Atom count atom_count	18528

MTDNNKYRDVEIRAPRGNKLTAKSWLTEAPLRMLMNNLDPQVAENPKELVVYGGIGRAARNWECYDKIVETLTRLEDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVPHWANWEHFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYGGSLKGKWVLTAGLGGMGGAQPLAATLAGACSLNIESQQSRIDFRLETRYVDEQATDLDDALVRIAKYTAEGKAISIALHGNAAEILPELVKRGVRPDMVTDQTSAHDPLNGYLPIGWTWEQYRDRAQTIPAAVVKAAKASMAVHVQAMLDFQKQGVPTFDYGNNIRQMAKEEGVANAFDFPGFVPAYIRPLFCRGVGPFRWAALSGEAEDIYKTDAKVKELIPDDAHLHRWLDMARERISFQGLPARICWVGLGLRAKLGLAFNEMVRSGELSAPVVIGRDHLDSGSVSSPNRETEAMRDGSDAVSDWPLLNALLNTAGGATWVSLHHGGGVGMGFSQHSGMVIVCDGTDEAAERIARVLTNDPGTGVMRHADAGYDIAIDCAKEQGLDLPMITG

Source Source

Source organism organism	PSEUDOMONAS PUTIDA
NCBI taxonomy ID ncbi_taxonomy_id	303
Host organism host_organism	ESCHERICHIA COLI

Entity 2 Entity 2 non-polymer

Description description	ACETATE ION
Molecular weight formula_weight	59.0 kDa
Source method src_method	syn
Copy count entity_count	7

Entity 3 Entity 3 non-polymer

Description description	GLYCEROL
Molecular weight formula_weight	92.1 kDa
Source method src_method	syn
Copy count entity_count	9

Entity 4 Entity 4 non-polymer

Description description	NICOTINAMIDE-ADENINE-DINUCLEOTIDE
Molecular weight formula_weight	663.4 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 5 Entity 5 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	1310

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6. Fold Classification (SCOP + CATH) 12 domains

SCOP 2.08 (4 domains)

Domain ID domain_id	d2v7ga_
Class class	e — Multi-domain proteins (alpha and beta)
Fold Fold fold	e.51 — Urocanase
Superfamily Superfamily superfamily	e.51.1 — Urocanase
Family Family family	e.51.1.1 — Urocanase

Domain ID domain_id	d2v7gb_
Class class	e — Multi-domain proteins (alpha and beta)
Fold Fold fold	e.51 — Urocanase
Superfamily Superfamily superfamily	e.51.1 — Urocanase
Family Family family	e.51.1.1 — Urocanase

Domain ID domain_id	d2v7gc_
Class class	e — Multi-domain proteins (alpha and beta)
Fold Fold fold	e.51 — Urocanase
Superfamily Superfamily superfamily	e.51.1 — Urocanase
Family Family family	e.51.1.1 — Urocanase

Domain ID domain_id	d2v7gd_
Class class	e — Multi-domain proteins (alpha and beta)
Fold Fold fold	e.51 — Urocanase
Superfamily Superfamily superfamily	e.51.1 — Urocanase
Family Family family	e.51.1.1 — Urocanase

CATH v4.4 (8 domains)

Domain ID domain_id	2v7gA01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	1770 — Urocanase fold
Homologous superfamily homologous superfamily	10 — Urocanase superfamily

Domain ID domain_id	2v7gA02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	10730 — Urocanase like domains

Domain ID domain_id	2v7gB01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	1770 — Urocanase fold
Homologous superfamily homologous superfamily	10 — Urocanase superfamily

Domain ID domain_id	2v7gB02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	10730 — Urocanase like domains

Domain ID domain_id	2v7gC01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	1770 — Urocanase fold
Homologous superfamily homologous superfamily	10 — Urocanase superfamily

Domain ID domain_id	2v7gC02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	10730 — Urocanase like domains

Domain ID domain_id	2v7gD01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	1770 — Urocanase fold
Homologous superfamily homologous superfamily	10 — Urocanase superfamily

Domain ID domain_id	2v7gD02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	10730 — Urocanase like domains

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Unit-cell length a length_a	68.068 Å
Unit-cell length b length_b	154.316 Å
Unit-cell length c length_c	216.391 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	2.000 Å
Low-resolution limit resolution_low	28.810 Å
R factor r_factor	0.1560
R_work r_work	0.1560
R_free r_free	0.1810

Crystal Structure of an Engineered Urocanase Tetramer

1. Protein Identity and Related Structures Protein Identity & Related Structures

UROCANATE HYDRATASE

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2v7g__assembly_3__model_1

2v7g__assembly_3__model_1 | I(q)

2v7g__assembly_3__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 12 domains

SCOP 2.08 (4 domains)

CATH v4.4 (8 domains)

7. Citations (1)

Crystal Structure of an Engineered Urocanase Tetramer

1. Protein Identity and Related Structures Protein Identity & Related Structures

UROCANATE HYDRATASE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2v7g__assembly_3__model_1

2v7g__assembly_3__model_1 | I(q)

2v7g__assembly_3__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 water

6. Fold Classification (SCOP + CATH) 12 domains

SCOP 2.08 (4 domains)

CATH v4.4 (8 domains)

7. Citations (1)