SAXSdb — 2x3a

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1. Protein Identity and Related Structures Protein Identity & Related Structures

TOXIC EXTRACELLULAR ENDOPEPTIDASE

AEROMONAS SALMONICIDA SUBSP. ACHROMOGENES

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 2	ZINC ION × 2 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 SULFATE ION × 6 GLYCEROL × 4 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q8GMV9_AERSA
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–343； UniProt 1–343

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2x3a
Deposition date deposition_date	2010-01-22
Structure title title	AsaP1 inactive mutant E294Q, an extracellular toxic zinc metalloendopeptidase
Keywords keywords	HYDROLASE; HYDROLASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2x3a__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2x3a__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

2x3a__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	27.40 Å
Rg (electron density)	26.40 Å
Total Rg	27.16 Å
Atom count	4586
Residues	586
Excluded volume	80391 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`2x3a__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Description description	TOXIC EXTRACELLULAR ENDOPEPTIDASE
Molecular weight formula_weight	37285.5 kDa
EC 编号 ec_number	3.4.24.39
Mutation mutation	YES
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	2523

MMKVTPIALLLAGVLASPLCAAGLDAQLTLVDGSTDDVRVNLTLTNTGDKPIRLLKWQLPGSDDAPLFLVERDGQPVSYEGALIKRAAPTDKDFQLLKAGQSLTVQAEVSGLYDMSAQGQYSIRYQLPARRKRPRAKQAQASESNAITLWVEGVNDERVQAKVAAAEPQAVAGSVSFSGRCTNTQKSDLLTALDAASGISNNASSYLAVDKPDGQRYRSWFGAYSSARWDQAETNFSKIKDAIDNKPLTFDCSCKQSYFAYVYPDQPYKVYLCKSFWTAPVTGSDSRAGTIVHQLSHFNVVAGTDDLGYGQANARNLAKTDPVKALNNADNHEYFAENTPSEN

Source Source

Source organism organism	AEROMONAS SALMONICIDA SUBSP. ACHROMOGENES
NCBI taxonomy ID ncbi_taxonomy_id	113288
Host organism host_organism	ESCHERICHIA COLI

Entity 2 Entity 2 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 3 Entity 3 non-polymer

Description description	2-(N-MORPHOLINO)-ETHANESULFONIC ACID
Molecular weight formula_weight	195.2 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 4 Entity 4 non-polymer

Description description	SULFATE ION
Molecular weight formula_weight	96.1 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 5 Entity 5 non-polymer

Description description	GLYCEROL
Molecular weight formula_weight	92.1 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 6 Entity 6 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	199

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6. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

Domain ID domain_id	2x3aA01
Class class	2 — Mainly Beta
Architecture architecture	60 — Sandwich
Topology topology	40 — Immunoglobulin-like
Homologous superfamily homologous superfamily	2970 —

Domain ID domain_id	2x3aA02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	390 — Collagenase (Catalytic Domain)
Homologous superfamily homologous superfamily	10 — Collagenase (Catalytic Domain)

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Unit-cell length a length_a	98.249 Å
Unit-cell length b length_b	74.468 Å
Unit-cell length c length_c	54.703 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	112.35 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	4

Space group space_group	C 1 2 1
Space-group number int_tables_number	5
Crystal system crystal_system	—

Resolution resolution	2.000 Å
Low-resolution limit resolution_low	57.600 Å
R factor r_factor	0.1720
R_work r_work	0.1701
R_free r_free	0.2046

AsaP1 inactive mutant E294Q, an extracellular toxic zinc metalloendopeptidase

1. Protein Identity and Related Structures Protein Identity & Related Structures

TOXIC EXTRACELLULAR ENDOPEPTIDASE

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2x3a__assembly_1__model_1

2x3a__assembly_1__model_1 | I(q)

2x3a__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 water

6. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

7. Citations (4)

AsaP1 inactive mutant E294Q, an extracellular toxic zinc metalloendopeptidase

1. Protein Identity and Related Structures Protein Identity & Related Structures

TOXIC EXTRACELLULAR ENDOPEPTIDASE

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

2x3a__assembly_1__model_1

2x3a__assembly_1__model_1 | I(q)

2x3a__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 water

6. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

7. Citations (4)