2xe0
Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus
1. Protein Identity and Related Structures Protein Identity & Related Structures
No usable UniProt protein identity is available for this entry.
The relationship tables retain this entry's assembly and composition data, but cross-PDB links for the same protein cannot be established reliably without a unified protein identity.
Assembly Composition of the Current Entry
| Assembly | Physical composition | Protein state | 蛋白 / DNA / RNA / 其他Polymer | Data consistency |
|---|---|---|---|---|
| 1 | Protein–DNA | Heteromer | 2 / 2 / 0 / 0 | Consistent with all polymers |
| 2 | Nucleic acid only | No protein | 0 / 2 / 0 / 0 | Consistent with all polymers |
| 3 | Protein heterocomplex | Heteromer | 2 / 0 / 0 / 0 | Consistent with protein count |
The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.
2. Structure Basics 2. Structure Basics
| Entry ID entry_id | 2xe0 |
| Deposition date deposition_date | 2010-05-10 |
| Structure title title | Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus |
| Keywords keywords | ;DNA BINDING PROTEIN-DNA COMPLEX, HOMING ENDONUCLEASES, DOUBLE-STRAND BREAK, HOMOLOGOUS RECOMBINATION, HUMAN RAG1 GENE, SEVERE COMBINED IMMUNODEFICIENCY (SCID), DNA BINDING PROTEIN ;; DNA BINDING PROTEIN/DNA |
| Experimental Method method | X-RAY DIFFRACTION |
3. Official assembly/model SAXS Official SAXS Profiles
This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.
2xe0__assembly_1__model_1
Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)
2xe0__assembly_1__model_1 | I(q)
2xe0__assembly_1__model_1 | P(r) · Pending
| Rg（Guinier） | 24.45 Å |
| Rg (electron density) | 23.84 Å |
| Total Rg | 24.56 Å |
| Atom count | 3409 |
| Residues | 352 |
| Excluded volume | 58004 ų |
| Maximum q | 0.500 Å⁻¹ |
| Assembly | Model | Structure unit | Oligomeric description | Status | CRYSOL | Actions |
|---|---|---|---|---|---|---|
| 1 | 1 | 2xe0__assembly_1__model_1 |
tetrameric （4） | Success | 4.1.3-1-20251215 (887e7ef) | View Download |
| 2 | 1 | 2xe0__assembly_2__model_1 |
dimeric （2） | Success | 4.1.3-1-20251215 (887e7ef) | View Download |
| 3 | 1 | 2xe0__assembly_3__model_1 |
dimeric （2） | Success | 4.1.3-1-20251215 (887e7ef) | View Download |
4. Crystallography and Experiment 4. Crystallography & Experiment
5. Entities and Polymers Entities & Polymers (8)
6. Fold Classification (SCOP + CATH) 4 domains
SCOP 2.08 (2 domains)
| Domain ID domain_id | d2xe0a_ |
| Class class | d — Alpha and beta proteins (a+b) |
| Fold Fold fold | d.95 — Homing endonuclease-like |
| Superfamily Superfamily superfamily | d.95.2 — Homing endonucleases |
| Family Family family | d.95.2.1 — Group I mobile intron endonuclease |
| Domain ID domain_id | d2xe0b_ |
| Class class | d — Alpha and beta proteins (a+b) |
| Fold Fold fold | d.95 — Homing endonuclease-like |
| Superfamily Superfamily superfamily | d.95.2 — Homing endonucleases |
| Family Family family | d.95.2.1 — Group I mobile intron endonuclease |
CATH v4.4 (2 domains)
| Domain ID domain_id | 2xe0A00 |
| Class class | 3 — Alpha Beta |
| Architecture architecture | 10 — Roll |
| Topology topology | 28 — Endonuclease I-creI |
| Homologous superfamily homologous superfamily | 10 — Homing endonucleases |
| Domain ID domain_id | 2xe0B00 |
| Class class | 3 — Alpha Beta |
| Architecture architecture | 10 — Roll |
| Topology topology | 28 — Endonuclease I-creI |
| Homologous superfamily homologous superfamily | 10 — Homing endonucleases |