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2yii

Manipulating the regioselectivity of phenylalanine aminomutase: new insights into the reaction mechanism of MIO-dependent enzymes from structure-guided directed evolution

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

PHENYLALANINE AMMONIA-LYASE

TAXUS WALLICHIANA VAR. CHINENSIS

UniProt Q68G84

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein homooligomer Homooligomer Protein 4 BETA-MERCAPTOETHANOL × 8 FORMIC ACID × 4 GLYCEROL × 1 water × 4 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer
2yii 4baa 4bab 4c5r 4c5s 4c5u 4c6g 4cq5 4v2q 4v2r

10 assemblies

Other homooligomers

None

Heteromeric or nucleic-acid complex

None

View Construct and Data Evidence
UniProt name Q68G83_TAXWC
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 21–705； UniProt 1–687 Author chain B； PDBConstruct 21–705； UniProt 1–687 Author chain C； PDBConstruct 21–705； UniProt 1–687 Author chain D； PDBConstruct 21–705； UniProt 1–687

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2yii
Deposition date deposition_date2011-05-13
Structure title titleManipulating the regioselectivity of phenylalanine aminomutase: new insights into the reaction mechanism of MIO-dependent enzymes from structure-guided directed evolution
Keywords keywordsLYASE; LYASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2yii__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2yii__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2yii__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）40.90 Å
Rg (electron density)40.23 Å
Total Rg40.69 Å
Atom count20131
Residues2581
Excluded volume360840 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2yii__assembly_1__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

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6. Fold Classification (SCOP + CATH) 16 domains

SCOP 2.08 (4 domains)

Domain ID domain_idd2yiia_
Class classa — All alpha proteins
Fold Fold folda.127 — L-aspartase-like
Superfamily Superfamily superfamilya.127.1 — L-aspartase-like
Family Family familya.127.1.0 — automated matches
Domain ID domain_idd2yiib_
Class classa — All alpha proteins
Fold Fold folda.127 — L-aspartase-like
Superfamily Superfamily superfamilya.127.1 — L-aspartase-like
Family Family familya.127.1.0 — automated matches
Domain ID domain_idd2yiic_
Class classa — All alpha proteins
Fold Fold folda.127 — L-aspartase-like
Superfamily Superfamily superfamilya.127.1 — L-aspartase-like
Family Family familya.127.1.0 — automated matches
Domain ID domain_idd2yiid_
Class classa — All alpha proteins
Fold Fold folda.127 — L-aspartase-like
Superfamily Superfamily superfamilya.127.1 — L-aspartase-like
Family Family familya.127.1.0 — automated matches

CATH v4.4 (12 domains)

Domain ID domain_id2yiiA01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology275 — Fumarase C; Chain B, domain 1
Homologous superfamily homologous superfamily10 — Fumarase/aspartase (N-terminal domain)
Domain ID domain_id2yiiA02
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology200 — Fumarase C; Chain A, domain 2
Homologous superfamily homologous superfamily10 — Fumarase/aspartase (Central domain)
Domain ID domain_id2yiiA03
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology274 — Enzyme I; Chain A, domain 2
Homologous superfamily homologous superfamily20 — Phenylalanine ammonia-lyase 1; domain 3
Domain ID domain_id2yiiB01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology275 — Fumarase C; Chain B, domain 1
Homologous superfamily homologous superfamily10 — Fumarase/aspartase (N-terminal domain)
Domain ID domain_id2yiiB02
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology200 — Fumarase C; Chain A, domain 2
Homologous superfamily homologous superfamily10 — Fumarase/aspartase (Central domain)
Domain ID domain_id2yiiB03
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology274 — Enzyme I; Chain A, domain 2
Homologous superfamily homologous superfamily20 — Phenylalanine ammonia-lyase 1; domain 3
Domain ID domain_id2yiiC01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology275 — Fumarase C; Chain B, domain 1
Homologous superfamily homologous superfamily10 — Fumarase/aspartase (N-terminal domain)
Domain ID domain_id2yiiC02
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology200 — Fumarase C; Chain A, domain 2
Homologous superfamily homologous superfamily10 — Fumarase/aspartase (Central domain)
Domain ID domain_id2yiiC03
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology274 — Enzyme I; Chain A, domain 2
Homologous superfamily homologous superfamily20 — Phenylalanine ammonia-lyase 1; domain 3
Domain ID domain_id2yiiD01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology275 — Fumarase C; Chain B, domain 1
Homologous superfamily homologous superfamily10 — Fumarase/aspartase (N-terminal domain)
Domain ID domain_id2yiiD02
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology200 — Fumarase C; Chain A, domain 2
Homologous superfamily homologous superfamily10 — Fumarase/aspartase (Central domain)
Domain ID domain_id2yiiD03
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology274 — Enzyme I; Chain A, domain 2
Homologous superfamily homologous superfamily20 — Phenylalanine ammonia-lyase 1; domain 3
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7. Citations (1)