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2zzd

Recombinant thiocyanate hydrolase, air-oxidized form of holo-enzyme

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Thiocyanate hydrolase subunit alpha

Thiobacillus thioparus

UniProt O66187

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 12 Thiocyanate hydrolase subunit beta × 4 （O66186） Thiocyanate hydrolase subunit gamma × 4 （O66188） beta-D-fructofuranose × 3 COBALT (III) ION × 4 L(+)-TARTARIC ACID × 4 beta-D-glucopyranose × 1 water × 12 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2dd4 2dd5 2dxb 2dxb 2dxc 2zzd 3vyg

7 assemblies

View Construct and Data Evidence
UniProt name SCNA_THITI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–126； UniProt 1–126 Author chain D； PDBConstruct 1–126； UniProt 1–126 Author chain G； PDBConstruct 1–126； UniProt 1–126 Author chain J； PDBConstruct 1–126； UniProt 1–126

Thiocyanate hydrolase subunit beta

Thiobacillus thioparus

UniProt O66186

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 12 Thiocyanate hydrolase subunit alpha × 4 （O66187） Thiocyanate hydrolase subunit gamma × 4 （O66188） beta-D-fructofuranose × 3 COBALT (III) ION × 4 L(+)-TARTARIC ACID × 4 beta-D-glucopyranose × 1 water × 12 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2dd4 2dd5 2dxb 2dxb 2dxc 2zzd 3vyg

7 assemblies

View Construct and Data Evidence
UniProt name SCNB_THITI
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–157； UniProt 1–157 Author chain E； PDBConstruct 1–157； UniProt 1–157 Author chain H； PDBConstruct 1–157； UniProt 1–157 Author chain K； PDBConstruct 1–157； UniProt 1–157

Thiocyanate hydrolase subunit gamma

Thiobacillus thioparus

UniProt O66188

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 12 Thiocyanate hydrolase subunit alpha × 4 （O66187） Thiocyanate hydrolase subunit beta × 4 （O66186） beta-D-fructofuranose × 3 COBALT (III) ION × 4 L(+)-TARTARIC ACID × 4 beta-D-glucopyranose × 1 water × 12 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2dd4 2dd5 2dxb 2dxb 2dxc 2zzd 3vyg

7 assemblies

View Construct and Data Evidence
UniProt name SCNC_THITI
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–243； UniProt 1–243 Author chain F； PDBConstruct 1–243； UniProt 1–243 Author chain I； PDBConstruct 1–243； UniProt 1–243 Author chain L； PDBConstruct 1–243； UniProt 1–243

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2zzd
Deposition date deposition_date2009-02-09
Structure title titleRecombinant thiocyanate hydrolase, air-oxidized form of holo-enzyme
Keywords keywords;SCNASE, HYDROLASE, COBALT, METALLOPROTEIN, SULFENIC ACID, SULFINIC ACID, NITRILE HYDRATASE, THIOCYANATE, CARBONYL SULFIDE, CLAW SETTING, PROTEIN, ENZYME, COMPLEX, MODEL COMPLEX, NON-CORRIN, POST-TRANSLATIONAL MODIFICATION, SULFENATE, SULFINATE, CYSTEINE, OXIDATION, AUTOCATALYTIC ACTIVATION, AIR INACTIVATION, Metal-binding ;; HYDROLASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2zzd__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2zzd__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2zzd__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）38.39 Å
Rg (electron density)37.74 Å
Total Rg38.29 Å
Atom count15841
Residues1951
Excluded volume281080 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2zzd__assembly_1__model_1 dodecameric （12） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (8)

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6. Fold Classification (SCOP + CATH) 12 domains

CATH v4.4 (12 domains)

Domain ID domain_id2zzdA00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily50 —
Domain ID domain_id2zzdB00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology472 — Cyclin A; domain 1
Homologous superfamily homologous superfamily20 — Nitrile hydratase, beta subunit
Domain ID domain_id2zzdC00
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology330 — Nitrile Hydratase; Chain A
Homologous superfamily homologous superfamily10 — Nitrile hydratase alpha /Thiocyanate hydrolase gamma
Domain ID domain_id2zzdD00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily50 —
Domain ID domain_id2zzdE00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology472 — Cyclin A; domain 1
Homologous superfamily homologous superfamily20 — Nitrile hydratase, beta subunit
Domain ID domain_id2zzdF00
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology330 — Nitrile Hydratase; Chain A
Homologous superfamily homologous superfamily10 — Nitrile hydratase alpha /Thiocyanate hydrolase gamma
Domain ID domain_id2zzdG00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily50 —
Domain ID domain_id2zzdH00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology472 — Cyclin A; domain 1
Homologous superfamily homologous superfamily20 — Nitrile hydratase, beta subunit
Domain ID domain_id2zzdI00
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology330 — Nitrile Hydratase; Chain A
Homologous superfamily homologous superfamily10 — Nitrile hydratase alpha /Thiocyanate hydrolase gamma
Domain ID domain_id2zzdJ00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily50 —
Domain ID domain_id2zzdK00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology472 — Cyclin A; domain 1
Homologous superfamily homologous superfamily20 — Nitrile hydratase, beta subunit
Domain ID domain_id2zzdL00
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology330 — Nitrile Hydratase; Chain A
Homologous superfamily homologous superfamily10 — Nitrile hydratase alpha /Thiocyanate hydrolase gamma
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7. Citations (1)