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3mn6

Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Actin-5C

Drosophila melanogaster

UniProt P10987

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Protein spire × 1 （Q9U1K1） CALCIUM ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 1 water × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 Protein spire × 1 （Q9U1K1） CALCIUM ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 1 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 Protein spire × 1 （Q9U1K1） CALCIUM ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 1 water × 2 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
2hf3 2hf4 3eks 3eku 3el2

5 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
3mmv 3mn6 3mn6 3mn6 3mn7 3mn9 4jhd 4jhd 4m63 4rwt 4rwt 5wfn

14 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name ACT1_DROME
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–374； UniProt 3–376 Author chain F； PDBConstruct 1–374； UniProt 3–376 Author chain K； PDBConstruct 1–374； UniProt 3–376

Protein spire

Drosophila melanogaster

UniProt Q9U1K1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Actin-5C × 1 （P10987） CALCIUM ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 1 water × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 Actin-5C × 1 （P10987） CALCIUM ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 1 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 Actin-5C × 1 （P10987） CALCIUM ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 1 water × 2 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
3mmv 3mn5 3mn6 3mn6 3mn6 3mn9 3ue5 3ue5 3ue5 4efh

10 assemblies

View Construct and Data Evidence
UniProt name SPIR_DROME
Isoform —
PDB entities 2
Chains and sequence ranges Author chain X； PDBConstruct 1–19； UniProt 396–485 Author chain Y； PDBConstruct 1–19； UniProt 396–485 Author chain Z； PDBConstruct 1–19； UniProt 396–485

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id3mn6
Deposition date deposition_date2010-04-21
Structure title titleStructures of actin-bound WH2 domains of Spire and the implication for filament nucleation
Keywords keywordsWH2 domain, Spire, Actin complex, Contractile Protein-Protein binding complex; Contractile Protein/Protein binding
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

3mn6__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

3mn6__assembly_2__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

3mn6__assembly_2__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）22.72 Å
Rg (electron density)21.79 Å
Total Rg22.56 Å
Atom count2944
Residues379
Excluded volume52274 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 3mn6__assembly_1__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 3mn6__assembly_2__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 3mn6__assembly_3__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

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6. Fold Classification (SCOP + CATH) 15 domains

SCOP 2.08 (6 domains)

Domain ID domain_idd3mn6a1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.1 — Actin/HSP70
Domain ID domain_idd3mn6a2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.1 — Actin/HSP70
Domain ID domain_idd3mn6f1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.1 — Actin/HSP70
Domain ID domain_idd3mn6f2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.1 — Actin/HSP70
Domain ID domain_idd3mn6k1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.1 — Actin/HSP70
Domain ID domain_idd3mn6k2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.1 — Actin/HSP70

CATH v4.4 (9 domains)

Domain ID domain_id3mn6A01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id3mn6A02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id3mn6A03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology640 — Actin; Chain A, domain 4
Homologous superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
Domain ID domain_id3mn6F01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id3mn6F02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id3mn6F03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology640 — Actin; Chain A, domain 4
Homologous superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
Domain ID domain_id3mn6K01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id3mn6K02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id3mn6K03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology640 — Actin; Chain A, domain 4
Homologous superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
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7. Citations (1)