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3q0a

X-ray crystal structure of the transcription initiation complex of the N4 mini-vRNAP with P2 promoter: Mismatch complex

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Virion RNA polymerase

Enterobacteria phage N4

UniProt Q859P9

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–DNA Monomer Protein 1 DNA 1 ;DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*A*GP*TP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3') ; × 1 PHOSPHATE ION × 1 GUANOSINE-5'-TRIPHOSPHATE × 1 water × 2 Consistent with all polymers
2 Protein–DNA Monomer Protein 1 DNA 1 ;DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*A*GP*TP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3') ; × 1 PHOSPHATE ION × 1 GUANOSINE-5'-TRIPHOSPHATE × 1 water × 2 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer
2po4 9pnq 9pnr 9pnt

4 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
3c2p 3c2p 3c3l 3c3l 3c46 3c46 3q0a 3q0a 3q22 3q22 3q23 3q23

24 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name Q859P9_BPN4
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 13–1118； UniProt 998–2103 Author chain B； PDBConstruct 13–1118； UniProt 998–2103

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id3q0a
Deposition date deposition_date2010-12-15
Structure title titleX-ray crystal structure of the transcription initiation complex of the N4 mini-vRNAP with P2 promoter: Mismatch complex
Keywords keywordsRNA polymerase, TRANSCRIPTION-DNA complex; TRANSCRIPTION/DNA
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

3q0a__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

3q0a__assembly_2__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

3q0a__assembly_2__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）32.48 Å
Rg (electron density)31.55 Å
Total Rg32.18 Å
Atom count8892
Residues1114
Excluded volume156850 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 3q0a__assembly_1__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 3q0a__assembly_2__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (5)

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6. Fold Classification (SCOP + CATH) 12 domains

CATH v4.4 (12 domains)

Domain ID domain_id3q0aA02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily2440 —
Domain ID domain_id3q0aA03
Class class6 — Special
Architecture architecture10 — Helix non-globular
Topology topology140 — Helix Hairpins
Homologous superfamily homologous superfamily1360 —
Domain ID domain_id3q0aA04
Class class6 — Special
Architecture architecture10 — Helix non-globular
Topology topology140 — Helix Hairpins
Homologous superfamily homologous superfamily1370 —
Domain ID domain_id3q0aA05
Class class6 — Special
Architecture architecture10 — Helix non-globular
Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily1860 —
Domain ID domain_id3q0aA06
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology750 — Transcription Regulator spoIIAA
Homologous superfamily homologous superfamily220 —
Domain ID domain_id3q0aA08
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology140 — Butyryl-CoA Dehydrogenase, subunit A; domain 3
Homologous superfamily homologous superfamily110 —
Domain ID domain_id3q0aB02
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily2440 —
Domain ID domain_id3q0aB03
Class class6 — Special
Architecture architecture10 — Helix non-globular
Topology topology140 — Helix Hairpins
Homologous superfamily homologous superfamily1360 —
Domain ID domain_id3q0aB04
Class class6 — Special
Architecture architecture10 — Helix non-globular
Topology topology140 — Helix Hairpins
Homologous superfamily homologous superfamily1370 —
Domain ID domain_id3q0aB05
Class class6 — Special
Architecture architecture10 — Helix non-globular
Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily1860 —
Domain ID domain_id3q0aB06
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology750 — Transcription Regulator spoIIAA
Homologous superfamily homologous superfamily220 —
Domain ID domain_id3q0aB08
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology140 — Butyryl-CoA Dehydrogenase, subunit A; domain 3
Homologous superfamily homologous superfamily110 —
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7. Citations (1)