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3t72

PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Phosphate regulon transcriptional regulatory protein phoB

Escherichia coli

UniProt P0AFJ5

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 3 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 RNA polymerase sigma factor rpoD, DNA-directed RNA polymerase subunit beta × 1 （E8Y6A0,P00579） Consistent with all polymers
10 Protein–DNA Homooligomer Protein 2 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
11 Protein–DNA Homooligomer Protein 2 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
12 Protein–DNA Homooligomer Protein 2 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
2 Insufficient information Heteromer Protein 3 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 RNA polymerase sigma factor rpoD, DNA-directed RNA polymerase subunit beta × 1 （E8Y6A0,P00579） Consistent with all polymers
3 Protein–DNA Homooligomer Protein 2 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
4 Protein–DNA Homooligomer Protein 2 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
5 Protein–DNA Homooligomer Protein 2 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
6 Protein–DNA Homooligomer Protein 2 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
7 Protein–DNA Homooligomer Protein 2 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
8 Protein–DNA Homooligomer Protein 2 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
9 Protein–DNA Homooligomer Protein 2 DNA 2 PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer
1gxq 2iyn 2iyn 2iyn 2jb9 2jb9

6 assemblies

Homodimer
2iyn 2iyn 2jba

3 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2z33 3t72 3t72 3t72 3t72 3t72 3t72 3t72 3t72 3t72 3t72 3t72

13 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name PHOB_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain 1； PDBConstruct 1–102； UniProt 128–229 Author chain 4； PDBConstruct 1–102； UniProt 128–229 Author chain 5； PDBConstruct 1–102； UniProt 128–229 Author chain 8； PDBConstruct 1–102； UniProt 128–229 Author chain 9； PDBConstruct 1–102； UniProt 128–229 Author chain A； PDBConstruct 1–102； UniProt 128–229 Author chain B； PDBConstruct 1–102； UniProt 128–229 Author chain E； PDBConstruct 1–102； UniProt 128–229 Author chain F； PDBConstruct 1–102； UniProt 128–229 Author chain I； PDBConstruct 1–102； UniProt 128–229 Author chain J； PDBConstruct 1–102； UniProt 128–229 Author chain M； PDBConstruct 1–102； UniProt 128–229 Author chain N； PDBConstruct 1–102； UniProt 128–229 Author chain R； PDBConstruct 1–102； UniProt 128–229 Author chain S； PDBConstruct 1–102； UniProt 128–229 Author chain V； PDBConstruct 1–102； UniProt 128–229 Author chain W； PDBConstruct 1–102； UniProt 128–229 Author chain Z； PDBConstruct 1–102； UniProt 128–229 Author chain c； PDBConstruct 1–102； UniProt 128–229 Author chain d； PDBConstruct 1–102； UniProt 128–229 Author chain g； PDBConstruct 1–102； UniProt 128–229 Author chain h； PDBConstruct 1–102； UniProt 128–229 Author chain k； PDBConstruct 1–102； UniProt 128–229 Author chain l； PDBConstruct 1–102； UniProt 128–229

RNA polymerase sigma factor rpoD, DNA-directed RNA polymerase subunit beta

Escherichia coli

UniProt E8Y6A0

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 3 DNA 2 Phosphate regulon transcriptional regulatory protein phoB × 2 （P0AFJ5） PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
2 Insufficient information Heteromer Protein 3 DNA 2 Phosphate regulon transcriptional regulatory protein phoB × 2 （P0AFJ5） PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
3t72 3t72

2 assemblies

View Construct and Data Evidence
UniProt name E8Y6A0_ECOKO
Isoform —
PDB entities 4
Chains and sequence ranges Author chain o； PDBConstruct 85–99； UniProt 896–910 Author chain q； PDBConstruct 85–99； UniProt 896–910

RNA polymerase sigma factor rpoD, DNA-directed RNA polymerase subunit beta

Escherichia coli

UniProt P00579

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 3 DNA 2 Phosphate regulon transcriptional regulatory protein phoB × 2 （P0AFJ5） PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers
2 Insufficient information Heteromer Protein 3 DNA 2 Phosphate regulon transcriptional regulatory protein phoB × 2 （P0AFJ5） PHO BOX DNA (STRAND 1) × 1 PHO BOX DNA (STRAND 2) × 1 Consistent with all polymers

Other States of the Same Protein in the Database

Monomer
1sig

1 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1tlh 2p7v 2p7v 3iyd 3t72 3t72 4jk1 4jk1 4jk2 4jk2 4jkr 4jkr

132 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RPOD_ECOLI
Isoform —
PDB entities 4
Chains and sequence ranges Author chain o； PDBConstruct 2–78； UniProt 533–609 Author chain q； PDBConstruct 2–78； UniProt 533–609

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id3t72
Deposition date deposition_date2011-07-29
Structure title titlePhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex
Keywords keywordsWINGED-HELIX MOTIF, TRANSCRIPTION ACTIVATION, DNA-BINDING, TRANSCRIPTION-DNA complex; TRANSCRIPTION/DNA
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

3t72__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

3t72__assembly_2__model_1 | I(q)

10-2 10-1 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

3t72__assembly_2__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）25.91 Å
Rg (electron density)26.31 Å
Total Rg26.84 Å
Atom count1060
Residues52
Excluded volume57690 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 3t72__assembly_1__model_1 pentameric （5） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 3t72__assembly_2__model_1 pentameric （5） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 3t72__assembly_3__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 3t72__assembly_4__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 3t72__assembly_5__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 3t72__assembly_6__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
7 1 3t72__assembly_7__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
8 1 3t72__assembly_8__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
9 1 3t72__assembly_9__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
10 1 3t72__assembly_10__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
11 1 3t72__assembly_11__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
12 1 3t72__assembly_12__model_1 tetrameric （4） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

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7. Citations (1)