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3zxf

High resolution structure of Human Galectin-7

Method: X-RAY DIFFRACTION Dmax: 70.2 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

GALECTIN-7

HOMO SAPIENS

UniProt P47929

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 1–136 Non-standard monomer：Yes (specific site not provided by mmCIF) ACT ACETATE ION × 1 X-RAY DIFFRACTION X-ray crystallization conditions：pH 8.1；50MM SODIUM PHOSPHATE, 300 MM NACL, 15% PEG3350, 20MM IMIDAZOLE, pH 8.1 Resolution 1.38 Å R-free 0.226
2 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain B； UniProt 1–136 Non-standard monomer：Yes (specific site not provided by mmCIF) No other associated polymer X-RAY DIFFRACTION X-ray crystallization conditions：pH 8.1；50MM SODIUM PHOSPHATE, 300 MM NACL, 15% PEG3350, 20MM IMIDAZOLE, pH 8.1 Resolution 1.38 Å R-free 0.226

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 3ZXF Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1BKZ CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 Deposited 1998-07-13 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–135（135 aa）
Chain B 1–135（135 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.1；CRYSTALS WERE GROWN USING THE HANGING DROP METHOD FROM DROPS CONTAINING 9 MG/ML PROTEIN AT PH 8.1 IN 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, AND 8.5% PEG 3350. DROPS WERE EQUILIBRATED AGAINST RESERVOIRS CONTAINING 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, AND 17% PEG 3350., vapor diffusion - hanging drop
Resolution 1.90 Å R-free 0.260
2GAL CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH GALACTOSE Deposited 1998-07-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–135（135 aa）
Chain B 1–135（135 aa）
Not recorded GAL beta-D-galactopyranose × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.1；CRYSTALS WERE GROWN USING THE HANGING DROP METHOD FROM DROPS CONTAINING 9 MG/ML PROTEIN AT PH 8.1 IN 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 8.5% PEG 3350 AND 25 MM GALACTOSE. DROPS WERE EQUILIBRATED AGAINST RESERVOIRS CONTAINING 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 17% PEG 3350 AND 50 MM GALACTOSE., vapor diffusion - hanging drop
Resolution 2.00 Å R-free 0.245
3GAL CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH GALACTOSAMINE Deposited 1998-07-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–135（135 aa）
Not recorded 1GN 2-amino-2-deoxy-beta-D-galactopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.1；CRYSTALS WERE GROWN USING THE HANGING DROP METHOD FROM DROPS CONTAINING 9 MG/ML PROTEIN AT PH 8.1 IN 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 8.5% PEG 3350 AND 25 MM GALACTOSAMINE. DROPS WERE EQUILIBRATED AGAINST RESERVOIRS CONTAINING 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 17% PEG 3350 AND 50 MM GALACTOSAMINE., vapor diffusion - hanging drop
Resolution 1.90 Å R-free 0.258
3GAL CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH GALACTOSAMINE Deposited 1998-07-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–135（135 aa）
Not recorded 1GN 2-amino-2-deoxy-beta-D-galactopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.1；CRYSTALS WERE GROWN USING THE HANGING DROP METHOD FROM DROPS CONTAINING 9 MG/ML PROTEIN AT PH 8.1 IN 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 8.5% PEG 3350 AND 25 MM GALACTOSAMINE. DROPS WERE EQUILIBRATED AGAINST RESERVOIRS CONTAINING 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 17% PEG 3350 AND 50 MM GALACTOSAMINE., vapor diffusion - hanging drop
Resolution 1.90 Å R-free 0.258
3ZXE Crystal structure of Human Galectin-7 in complex with a galactose- benzylphosphate inhibitor Deposited 2011-08-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 4–136（133 aa） Fragment：RESIDUES 4-136
Not recorded PGZ METHYL 2-O-[(S)-(BENZYLOXY)(HYDROXY)PHOSPHORYL]-3-DEOXY-3-{[(4-METHYLPHENYL)CARBONYL]AMINO}-1-THIO-BETA-D-GALACTOPYRANOSIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；0.1M BISTRISPROPANE, 20% PEG3350, pH 8.5
Resolution 1.67 Å R-free 0.236
3ZXE Crystal structure of Human Galectin-7 in complex with a galactose- benzylphosphate inhibitor Deposited 2011-08-10 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 4–136（133 aa） Fragment：RESIDUES 4-136
Not recorded PGZ METHYL 2-O-[(S)-(BENZYLOXY)(HYDROXY)PHOSPHORYL]-3-DEOXY-3-{[(4-METHYLPHENYL)CARBONYL]AMINO}-1-THIO-BETA-D-GALACTOPYRANOSIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；0.1M BISTRISPROPANE, 20% PEG3350, pH 8.5
Resolution 1.67 Å R-free 0.236
4GAL CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH LACTOSE Deposited 1998-07-13 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–135（135 aa）
Chain B 1–135（135 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.1；CRYSTALS WERE GROWN USING THE HANGING DROP METHOD FROM DROPS CONTAINING 9 MG/ML PROTEIN AT PH 8.1 IN 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 8.5% PEG 3350. DROPS WERE EQUILIBRATED AGAINST RESERVOIRS CONTAINING 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 17% PEG 3350. GALECTIN-7 CRYSTALS WERE THEN SOAKED WITH 5 MM LACTOSE FOR 2 HRS., vapor diffusion - hanging drop
Resolution 1.95 Å R-free 0.255
4UW3 Human galectin-7 in complex with a galactose based dendron D1. Deposited 2014-08-08 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–136（136 aa）
Not recorded 50G DENDRON-D1 × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 0.05 M BIS TRIS PROPANE PH7, 17 % PEG 3350
Resolution 1.48 Å R-free 0.198
4UW3 Human galectin-7 in complex with a galactose based dendron D1. Deposited 2014-08-08 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–136（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 0.05 M BIS TRIS PROPANE PH7, 17 % PEG 3350
Resolution 1.48 Å R-free 0.198
4UW4 Human galectin-7 in complex with a galactose based dendron D2-1. Deposited 2014-08-08 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–136（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；0.05 M BIS TRIS PROPANE PH 7, 17% PEG 3350
Resolution 1.77 Å R-free 0.235
4UW4 Human galectin-7 in complex with a galactose based dendron D2-1. Deposited 2014-08-08 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–136（136 aa）
Not recorded 4S0 DENDRON D2-1 × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；0.05 M BIS TRIS PROPANE PH 7, 17% PEG 3350
Resolution 1.77 Å R-free 0.235
4UW5 Human galectin-7 in complex with a galactose based dendron D2-2. Deposited 2014-08-08 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–136（136 aa）
Not recorded 4S0 DENDRON D2-1 × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 0.05M BIS TRIS PROPANE PH7, 14% PEG 3350
Resolution 2.04 Å R-free 0.319
4UW5 Human galectin-7 in complex with a galactose based dendron D2-2. Deposited 2014-08-08 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–136（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 0.05M BIS TRIS PROPANE PH7, 14% PEG 3350
Resolution 2.04 Å R-free 0.319
4UW5 Human galectin-7 in complex with a galactose based dendron D2-2. Deposited 2014-08-08 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–136（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 0.05M BIS TRIS PROPANE PH7, 14% PEG 3350
Resolution 2.04 Å R-free 0.319
4UW5 Human galectin-7 in complex with a galactose based dendron D2-2. Deposited 2014-08-08 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 1–136（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 0.05M BIS TRIS PROPANE PH7, 14% PEG 3350
Resolution 2.04 Å R-free 0.319
4UW5 Human galectin-7 in complex with a galactose based dendron D2-2. Deposited 2014-08-08 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain E 1–136（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 0.05M BIS TRIS PROPANE PH7, 14% PEG 3350
Resolution 2.04 Å R-free 0.319
4UW5 Human galectin-7 in complex with a galactose based dendron D2-2. Deposited 2014-08-08 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain F 1–136（136 aa）
Not recorded 4S0 DENDRON D2-1 × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 0.05M BIS TRIS PROPANE PH7, 14% PEG 3350
Resolution 2.04 Å R-free 0.319
4UW6 Human galectin-7 in complex with a galactose based dendron D3 Deposited 2014-08-08 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–136（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 0.05M BISTRIS PROPANE PH7, 17% PEG 3350
Resolution 1.79 Å R-free 0.251
4UW6 Human galectin-7 in complex with a galactose based dendron D3 Deposited 2014-08-08 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–136（136 aa）
Not recorded VV7 DENDRON-D3 × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 0.05M BISTRIS PROPANE PH7, 17% PEG 3350
Resolution 1.79 Å R-free 0.251
4XBQ Crystal Structure of Human Galectin-7 in Complex with Type 1 N-acetyllactosamine Deposited 2014-12-17 Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–136（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1M Hepes pH 7.5, 0.2M Lithium Sulfate, 0.1M Sodium Acetate, 25% (w/v) PEG 4000, temperature 298K
Resolution 2.23 Å R-free 0.225
4XBQ Crystal Structure of Human Galectin-7 in Complex with Type 1 N-acetyllactosamine Deposited 2014-12-17 Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–136（136 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1M Hepes pH 7.5, 0.2M Lithium Sulfate, 0.1M Sodium Acetate, 25% (w/v) PEG 4000, temperature 298K
Resolution 2.23 Å R-free 0.225
4Y26 Complex of human Galectin-7 and Galbeta1-3(6OSO3)GlcNAc Deposited 2015-02-09 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 4–136（133 aa）
Chain B 4–136（133 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1M HEPES (pH 7.5), 0.2M Li2SO4, 0.1M NaOAc, 25% (w/v) PEG 4000
Resolution 2.61 Å R-free 0.258
5GAL CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH N-ACETYLLACTOSAMINE Deposited 1998-07-14 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–135（135 aa）
Chain B 1–135（135 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.1；CRYSTALS WERE GROWN USING THE HANGING DROP METHOD FROM DROPS CONTAINING 9 MG/ML PROTEIN AT PH 8.1 IN 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 8.5% PEG 3350. DROPS WERE EQUILIBRATED AGAINST RESERVOIRS CONTAINING 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 17% PEG 3350. GALECTIN-7 CRYSTALS WERE THEN SOAKED WITH 50 MM N-ACETYLLACTOSAMINE FOR 3 DAYS., vapor diffusion - hanging drop
Resolution 2.00 Å R-free 0.296
5H9Q Crystal Structure of Human Galectin-7 in Complex with TD139 Deposited 2015-12-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–136（136 aa）
Chain B 1–136（136 aa）
Not recorded TD2 3-deoxy-3-[4-(3-fluorophenyl)-1H-1,2,3-triazol-1-yl]-beta-D-galactopyranosyl 3-deoxy-3-[4-(3-fluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-beta-D-galactopyranoside × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.22 M Mg Acetate, 21% (w/v) PEG3350
Resolution 1.93 Å R-free 0.238
5H9S Crystal Structure of Human Galectin-7 in Complex with TAZTDG Deposited 2015-12-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–136（136 aa）
Chain B 1–136（136 aa）
Not recorded TGZ (2~{S},3~{R},4~{S},5~{R},6~{R})-2-[(2~{S},3~{R},4~{S},5~{R},6~{R})-4-[4-(3-fluorophenyl)-1,2,3-triazol-1-yl]-6-(hydroxymethyl)-3,5-bis(oxidanyl)oxan-2-yl]sulfanyl-6-(hydroxymethyl)oxane-3,4,5-triol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.22 M MgAcetate, 21% (w/v) PEG 3350
Resolution 1.82 Å R-free 0.227
6VTO Crystal structure of human Galectin-7 in complex with 4-O-beta-D-Galactopyranosyl-D-glucose Deposited 2020-02-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Not recorded LBL (2~{R},3~{R},4~{R},5~{R})-4-[(2~{S},3~{R},4~{S},5~{R},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2,3,5, 6-tetrakis(oxidanyl)hexanal × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295 K；0.1 M Sodium Chloride, 0.1 M Tris pH 8, 20 % PEG3350, 5 % Glycerol
Resolution 1.69 Å R-free 0.215
6VTP Crystal structure of G16C human Galectin-7 mutant Deposited 2020-02-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：G16C Mutation：G16C GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M Sodium chloride, 0.1M Tris pH 8, 20 % PEG 3350, 15 % Glycerol
Resolution 2.30 Å R-free 0.261
6VTQ Crystal structure of G16C human Galectin-7 mutant in complex with lactose Deposited 2020-02-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：G16C Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：G16C Non-standard monomer：Yes (specific site not provided by mmCIF) GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；295.15 K；0.1 M Tris pH 8, 0.1 M Sodium Chloride, 25 % PEG3350, 7.5 mM alpha-lactose
Resolution 1.95 Å R-free 0.244
6VTR Crystal structure of G16S human Galectin-7 mutant Deposited 2020-02-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：G16S Mutation：G16S TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8.0, 20 % PEG 3350, 17.5 % Glycerol
Resolution 2.30 Å R-free 0.256
6VTS Crystal structure of G16S human Galectin-7 mutant in complex with lactose Deposited 2020-02-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：G16S Mutation：G16S GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；295.15 K；0.1 M Tris pH 8, 0.1 M NaCl, 16 % PEG 3350, 150 mM alpha-Lactose
Resolution 1.90 Å R-free 0.221
7N4O Crystal structure of R20A human Galectin-7 mutant Deposited 2021-06-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：R20A Mutation：R20A Non-standard monomer：Yes (specific site not provided by mmCIF) EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000 , 20% Glycerol
Resolution 2.05 Å R-free 0.252
7N57 Crystal structure of R20A human Galectin-7 mutant in presence of lactose Deposited 2021-06-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：R20A Mutation：R20A GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293.15 K；0.2M Sodium chloride, 0.1M Tris pH 8.0, 20% w/v PEG 6000
Resolution 1.83 Å R-free 0.208
7N6C Crystal structure of R22A human Galectin-7 mutant Deposited 2021-06-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：R22A Mutation：R22A GOL GLYCEROL × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 EDO 1,2-ETHANEDIOL × 5 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000, 17.5% Glycerol
Resolution 2.10 Å R-free 0.235
7N8D Crystal structure of R22A human Galectin-7 mutant in presence of Lactose Deposited 2021-06-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：R22A Mutation：R22A Non-standard monomer：Yes (specific site not provided by mmCIF) EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000, 7.5 mM alpha Lactose
Resolution 2.49 Å R-free 0.281
7N8G Crystal structure of R20A-R22A human Galectin-7 mutant Deposited 2021-06-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：R20A, R22A Mutation：R20A, R22A EDO 1,2-ETHANEDIOL × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 GOL GLYCEROL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000 ,17.5% Glycerol
Resolution 1.95 Å R-free 0.204
7N96 Crystal structure of D103A human Galectin-7 mutant Deposited 2021-06-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：D103A Mutation：D103A GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000 ,15% Glycerol
Resolution 2.38 Å R-free 0.259
7RDG Crystal structure of D103A human Galectin-7 mutant in presence of lactose Deposited 2021-07-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 2–136（135 aa）
Chain D 2–136（135 aa）
Mutation：D103A Mutation：D103A EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.3；295.15 K；0.1 M Tris pH 7.3, 0.1 M NaCl, 18% P400, 26% PEG 3350
Resolution 3.00 Å R-free 0.268
7RDG Crystal structure of D103A human Galectin-7 mutant in presence of lactose Deposited 2021-07-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：D103A Mutation：D103A EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.3；295.15 K；0.1 M Tris pH 7.3, 0.1 M NaCl, 18% P400, 26% PEG 3350
Resolution 3.00 Å R-free 0.268
7TKW Crystal structure of R14A human Galectin-7 mutant Deposited 2022-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：R14A Mutation：R14A GOL GLYCEROL × 4 PEG DI(HYDROXYETHYL)ETHER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.2 M Sodium chloride, 0.1 M Tris 8.0, 20 % w/v PEG 6000
Resolution 1.85 Å R-free 0.231
7TKX Crystal structure of R14A human Galectin-7 mutant in presence of 4-O-beta-D-Galactopyranosyl-D-glucose Deposited 2022-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：R14A Mutation：R14A LBL (2~{R},3~{R},4~{R},5~{R})-4-[(2~{S},3~{R},4~{S},5~{R},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2,3,5, 6-tetrakis(oxidanyl)hexanal × 2 EDO 1,2-ETHANEDIOL × 4 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000, 10% Glycerol
Resolution 1.83 Å R-free 0.213
7TKY Crystal structure of D94A human Galectin-7 mutant Deposited 2022-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：D94A Mutation：D94A GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000, 15% Glycerol
Resolution 2.53 Å R-free 0.283
7TKZ Crystal structure of D94A human Galectin-7 mutant in presence of lactose Deposited 2022-01-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：D94A Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：D94A Non-standard monomer：Yes (specific site not provided by mmCIF) EDO 1,2-ETHANEDIOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8.0, 20 % PEG 6000, 15% glycerol, 7.5 mM Lactose
Resolution 1.83 Å R-free 0.225
7TRN Crystal structure of R14A-R20A human Galectin-7 mutant Deposited 2022-01-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：R14A, R20A Mutation：R14A, R20A GOL GLYCEROL × 3 EDO 1,2-ETHANEDIOL × 11 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000, 17.5% Glycerol
Resolution 1.95 Å R-free 0.225
7TRN Crystal structure of R14A-R20A human Galectin-7 mutant Deposited 2022-01-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 2–136（135 aa）
Chain D 2–136（135 aa）
Mutation：R14A, R20A Mutation：R14A, R20A EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000, 17.5% Glycerol
Resolution 1.95 Å R-free 0.225
7TRO Crystal structure of R14A-R20A human Galectin-7 mutant in presence of lactose Deposited 2022-01-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–136（135 aa）
Chain B 2–136（135 aa）
Mutation：R14A, R20A Mutation：R14A, R20A EDO 1,2-ETHANEDIOL × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；295.15 K；0.1 M NaCl,0.1 M Tris pH 8, 20 % PEG 6000, 5% Glycerol, 7.5 mM Lactose
Resolution 1.80 Å R-free 0.221
7XAC Dimeric structure of human galectin-7 in complex with two glycerol Deposited 2022-03-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–136（136 aa）
Chain B 1–136（136 aa）
Not recorded GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；20% PEG 3350, 0.2M Magnesium chloride, 0.1M Hepes pH7.5
Resolution 1.80 Å R-free 0.253
7XBL Dimeric structure of human galectin-7 in complex with three glycerol Deposited 2022-03-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–136（136 aa）
Chain B 1–136（136 aa）
Not recorded GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.1M Hepes pH7.5, 12% PEG 3350
Resolution 2.00 Å R-free 0.222

34 other PDB entries and 47 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LEG7_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 3–138； UniProt 1–136 Author chain B； PDBConstruct 3–138； UniProt 1–136

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 3zxf

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 3zxf
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2. Structure Basics 2. Structure Basics

Entry ID entry_id3zxf
Deposition date deposition_date2011-08-10
Structure title titleHigh resolution structure of Human Galectin-7
Keywords keywordsSUGAR BINDING PROTEIN; SUGAR BINDING PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier21.60 Å
Radius of gyration Rg (electron density) rg_electron20.19 Å
Forward intensity I(0) i016949200.00
Molecular weight molecular_weight30264.0 kDa
Excluded volume excluded_volume37588 ų
Envelope volume envelope_volume45748 ų
Hydration-shell volume shell_volume19355 ų
Envelope diameter envelope_diameter71.7 Å
Shell Rg shell_rg26.25 Å
Envelope Rg envelope_rg20.52 Å
Shape Rg shape_rg20.15 Å
Total Rg total_rg21.13 Å
Total atoms total_atoms2141
Residues n_residues271
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax70.2 Å
Rg (real space) rg_real21.58 Å
Rg uncertainty (real space) rg_real_error0.41 Å
I(0) (real space) i0_real1.6950e+07
I(0) uncertainty (real space) i0_real_error2.1200e+05
Rg (reciprocal space) rg_reciprocal21.58 Å
I(0) (reciprocal space) i0_reciprocal16950000.0000
Solution quality estimate total_estimate0.8907
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary23.7 Å
Skewness Skewness skewness0.306
Kurtosis Kurtosis kurtosis-0.490
Angular range angular_range— – 0.3700 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha3536000.0000
Real-space data points n_real_points69
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.863; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.998
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 5 domains

SCOP 2.08 (3 domains)

Domain ID domain_idd3zxfa_
Class classb — All beta proteins
Fold Fold foldb.29 — Concanavalin A-like lectins/glucanases
Superfamily Superfamily superfamilyb.29.1 — Concanavalin A-like lectins/glucanases
Family Family familyb.29.1.3 — Galectin (animal S-lectin)
Domain ID domain_idd3zxfb1
Class classb — All beta proteins
Fold Fold foldb.29 — Concanavalin A-like lectins/glucanases
Superfamily Superfamily superfamilyb.29.1 — Concanavalin A-like lectins/glucanases
Family Family familyb.29.1.3 — Galectin (animal S-lectin)
Domain ID domain_idd3zxfb2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags

CATH v4.4 (2 domains)

Domain ID domain_id3zxfA00
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology120 — Jelly Rolls
Homologous superfamily homologous superfamily200 —
Domain ID domain_id3zxfB00
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology120 — Jelly Rolls
Homologous superfamily homologous superfamily200 —
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8. Citations (1)

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9. Files and Curves (10)