4a98

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromoflurazepam

Method: X-RAY DIFFRACTION

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

CYS-LOOP LIGAND-GATED ION CHANNEL

ERWINIA CHRYSANTHEMI

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	蛋白同源多聚体	同源多聚体	蛋白 5	7-BROMO-1-[2-(DIETHYLAMINO)ETHYL]-5-(2-FLUOROPHENYL)-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 5	与蛋白数一致
2	蛋白同源多聚体	同源多聚体	蛋白 5	7-BROMO-1-[2-(DIETHYLAMINO)ETHYL]-5-(2-FLUOROPHENYL)-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 5	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	ELIC_ERWCH
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–307； UniProt 11–316 作者链 B； PDB构建体 1–307； UniProt 11–316 作者链 C； PDB构建体 1–307； UniProt 11–316 作者链 D； PDB构建体 1–307； UniProt 11–316 作者链 E； PDB构建体 1–307； UniProt 11–316 作者链 F； PDB构建体 1–307； UniProt 11–316 作者链 G； PDB构建体 1–307； UniProt 11–316 作者链 H； PDB构建体 1–307； UniProt 11–316 作者链 I； PDB构建体 1–307； UniProt 11–316 作者链 J； PDB构建体 1–307； UniProt 11–316

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	4a98
沉积日期 deposition_date	2011-11-24
结构标题 title	X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromoflurazepam
关键词 keywords	TRANSPORT PROTEIN, CYS-LOOP RECEPTOR, GABA-A RECEPTOR, BENZODIAZEPINE; TRANSPORT PROTEIN
实验方法 method	X-RAY DIFFRACTION

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3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

4a98__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

4a98__assembly_1__model_1 | I(q)

100 个绘图点；横轴和纵轴均采用对数尺度。

4a98__assembly_1__model_1 | P(r) · Pending

P(r) 将按相同 assembly/model 分别计算和展示。

Rg（Guinier）	37.67 Å
Rg（电子密度）	36.76 Å
总 Rg	37.36 Å
原子数	12560
残基数	1535
排除体积	225360 Å³
最大 q	0.500 Å⁻¹

Assembly	Model	结构单元	聚集说明	状态	CRYSOL	操作
1	1	`4a98__assembly_1__model_1`	pentameric （5）	成功	4.1.3-1-20251215 (887e7ef)	查看下载
2	1	`4a98__assembly_2__model_1`	pentameric （5）	成功	4.1.3-1-20251215 (887e7ef)	查看下载

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合物信息 Entities & Polymers (2)

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6. 折叠分类 (SCOP + CATH) 20 domains

CATH v4.4 (20 domains)

结构域编号 domain_id	4a98A01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	70 — Distorted Sandwich
拓扑 Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
同源超家族 H-superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

结构域编号 domain_id	4a98A02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

结构域编号 domain_id	4a98B01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	70 — Distorted Sandwich
拓扑 Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
同源超家族 H-superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

结构域编号 domain_id	4a98B02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

结构域编号 domain_id	4a98C01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	70 — Distorted Sandwich
拓扑 Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
同源超家族 H-superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

结构域编号 domain_id	4a98C02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

结构域编号 domain_id	4a98D01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	70 — Distorted Sandwich
拓扑 Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
同源超家族 H-superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

结构域编号 domain_id	4a98D02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

结构域编号 domain_id	4a98E01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	70 — Distorted Sandwich
拓扑 Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
同源超家族 H-superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

结构域编号 domain_id	4a98E02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

结构域编号 domain_id	4a98F01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	70 — Distorted Sandwich
拓扑 Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
同源超家族 H-superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

结构域编号 domain_id	4a98F02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

结构域编号 domain_id	4a98G01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	70 — Distorted Sandwich
拓扑 Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
同源超家族 H-superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

结构域编号 domain_id	4a98G02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

结构域编号 domain_id	4a98H01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	70 — Distorted Sandwich
拓扑 Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
同源超家族 H-superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

结构域编号 domain_id	4a98H02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

结构域编号 domain_id	4a98I01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	70 — Distorted Sandwich
拓扑 Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
同源超家族 H-superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

结构域编号 domain_id	4a98I02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

结构域编号 domain_id	4a98J01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	70 — Distorted Sandwich
拓扑 Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
同源超家族 H-superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

结构域编号 domain_id	4a98J02
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

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晶胞边长 a length_a	106.400 Å
晶胞边长 b length_b	268.200 Å
晶胞边长 c length_c	111.400 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	108.10 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	20

空间群 space_group	P 1 21 1
空间群编号 int_tables_number	4
晶系 crystal_system	—

分辨率 resolution	3.610 Å
最低分辨率 resolution_low	25.470 Å
R 因子 r_factor	0.2314
R_work r_work	0.2306
R_free r_free	0.2454

描述 description	CYS-LOOP LIGAND-GATED ION CHANNEL
分子量 formula_weight	35383.4 kDa
来源方法 src_method	man
拷贝数 entity_count	10

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G,H,I,J
原子数 atom_count	25120

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromoflurazepam

1. 蛋白身份与相关结构 Protein Identity & Related Structures

CYS-LOOP LIGAND-GATED ION CHANNEL

当前结构中的状态

数据库中的同蛋白其他状态

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

4a98__assembly_1__model_1

4a98__assembly_1__model_1 | I(q)

4a98__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (2)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

6. 折叠分类 (SCOP + CATH) 20 domains

CATH v4.4 (20 domains)

7. 引用文献 (1)

来源生物 organism	ERWINIA CHRYSANTHEMI
NCBI 分类编号 ncbi_taxonomy_id	556
宿主生物 host_organism	ESCHERICHIA COLI

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromoflurazepam

1. 蛋白身份与相关结构 Protein Identity & Related Structures

CYS-LOOP LIGAND-GATED ION CHANNEL

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

4a98__assembly_1__model_1

4a98__assembly_1__model_1 | I(q)

4a98__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (2)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

6. 折叠分类 (SCOP + CATH) 20 domains

CATH v4.4 (20 domains)

7. 引用文献 (1)