4b9q

Open conformation of ATP-bound Hsp70 homolog DnaK

Method: X-RAY DIFFRACTION Dmax: 190.2 Å Quality: GOOD

1. 蛋白身份与相关结构 Protein Identity & Related Structures

CHAPERONE PROTEIN DNAK

ESCHERICHIA COLI

UniProt P0A6Y8

当前结构中的状态

Assembly 聚集状态 构建体 突变与修饰 配体、离子与共同组分 实验方法与环境 结构质量
1 蛋白单体 单体 蛋白 × 1 PDB 声明:monomeric(1) 与蛋白拷贝数一致 链 A; UniProt 1–605 突变:YES 非标准单体:是(mmCIF未提供具体位点) ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION X-ray结晶条件:pH 7.6;291 K;28% PEG4000, 5% GLYCEROL, 0.1 M AMMONIUM ACETATE, 0.085 M SODIUM CITRATE PH 5.6, 5 MM ATP, 10 MM MGCL2, 291 K 分辨率 2.40 Å R-free 0.239
2 蛋白单体 单体 蛋白 × 1 PDB 声明:monomeric(1) 与蛋白拷贝数一致 链 B; UniProt 1–605 突变:YES 非标准单体:是(mmCIF未提供具体位点) ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION X-ray结晶条件:pH 7.6;291 K;28% PEG4000, 5% GLYCEROL, 0.1 M AMMONIUM ACETATE, 0.085 M SODIUM CITRATE PH 5.6, 5 MM ATP, 10 MM MGCL2, 291 K 分辨率 2.40 Å R-free 0.239
3 蛋白单体 单体 蛋白 × 1 PDB 声明:monomeric(1) 与蛋白拷贝数一致 链 C; UniProt 1–605 突变:YES 非标准单体:是(mmCIF未提供具体位点) ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION X-ray结晶条件:pH 7.6;291 K;28% PEG4000, 5% GLYCEROL, 0.1 M AMMONIUM ACETATE, 0.085 M SODIUM CITRATE PH 5.6, 5 MM ATP, 10 MM MGCL2, 291 K 分辨率 2.40 Å R-free 0.239
4 蛋白单体 单体 蛋白 × 1 PDB 声明:monomeric(1) 与蛋白拷贝数一致 链 D; UniProt 1–605 突变:YES 非标准单体:是(mmCIF未提供具体位点) ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION X-ray结晶条件:pH 7.6;291 K;28% PEG4000, 5% GLYCEROL, 0.1 M AMMONIUM ACETATE, 0.085 M SODIUM CITRATE PH 5.6, 5 MM ATP, 10 MM MGCL2, 291 K 分辨率 2.40 Å R-free 0.239

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly, “相对当前条目”直接指出证据层面的不同;没有差异标签表示当前已读取字段一致。

共 52 个其他 PDB 条目、122 个 assembly。 打开独立比较页并筛选聚集状态

查看构建体与数据证据
UniProt名称 DNAK_ECOLI
Isoform
PDB实体 1
链与序列区间 作者链 A; PDB构建体 1–605; UniProt 1–605 作者链 B; PDB构建体 1–605; UniProt 1–605 作者链 C; PDB构建体 1–605; UniProt 1–605 作者链 D; PDB构建体 1–605; UniProt 1–605

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

SAXS profile for 4b9q

P(r) 距离分布 P(r) Distribution

P(r) distribution for 4b9q
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2. 结构基本信息 2. Structure Basics

条目编号 entry_id4b9q
沉积日期 deposition_date2012-09-06
结构标题 titleOpen conformation of ATP-bound Hsp70 homolog DnaK
关键词 keywordsCHAPERONE; CHAPERONE
实验方法 methodX-RAY DIFFRACTION

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier57.70
回转半径 Rg (电子) rg_electron58.07
零角强度 I(0) i01075820000.00
分子量 molecular_weight263140.0 kDa
排除体积 excluded_volume324500 ų
包络体积 envelope_volume494030 ų
水化壳体积 shell_volume73040 ų
包络直径 envelope_diameter205.3
壳层 Rg shell_rg57.25
包络 Rg envelope_rg56.90
形状 Rg shape_rg58.05
总 Rg total_rg58.10
总原子数 total_atoms18246
残基数 n_residues2332
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax190.2
Rg (实空间) rg_real58.20
Rg 误差 (实空间) rg_real_error1.99
I(0) (实空间) i0_real1.0760e+09
I(0) 误差 (实空间) i0_real_error2.0950e+07
Rg (倒空间) rg_reciprocal57.23
I(0) (倒空间) i0_reciprocal1074000000.0000
解质量估计 total_estimate0.7858
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary42.3
偏度 Skewness skewness0.449
峰度 Kurtosis kurtosis-0.594
角度范围 angular_range— – 0.1350 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha96350000.0000
实空间数据点数 n_real_points28
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.767; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.784; Smooth: 0.127

5. 晶体学与实验 5. Crystallography & Experiment

6. 实体与聚合物信息 Entities & Polymers (4)

7. 折叠分类 (SCOP + CATH) 20 domains

CATH v4.4 (20 domains)

结构域编号 domain_id4b9qA01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily40 — ATPase, nucleotide binding domain
结构域编号 domain_id4b9qA02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily40 — ATPase, nucleotide binding domain
结构域编号 domain_id4b9qA03
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id4b9qA04
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology34 — Substrate Binding Domain Of DNAk; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — Substrate Binding Domain Of DNAk; Chain A, domain 1
结构域编号 domain_id4b9qA05
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1270 — Substrate Binding Domain Of Dnak; Chain:A; Domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id4b9qB01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily40 — ATPase, nucleotide binding domain
结构域编号 domain_id4b9qB02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily40 — ATPase, nucleotide binding domain
结构域编号 domain_id4b9qB03
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id4b9qB04
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology34 — Substrate Binding Domain Of DNAk; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — Substrate Binding Domain Of DNAk; Chain A, domain 1
结构域编号 domain_id4b9qB05
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1270 — Substrate Binding Domain Of Dnak; Chain:A; Domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id4b9qC01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily40 — ATPase, nucleotide binding domain
结构域编号 domain_id4b9qC02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily40 — ATPase, nucleotide binding domain
结构域编号 domain_id4b9qC03
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id4b9qC04
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology34 — Substrate Binding Domain Of DNAk; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — Substrate Binding Domain Of DNAk; Chain A, domain 1
结构域编号 domain_id4b9qC05
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1270 — Substrate Binding Domain Of Dnak; Chain:A; Domain 2
同源超家族 H-superfamily homologous superfamily10
结构域编号 domain_id4b9qD01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily40 — ATPase, nucleotide binding domain
结构域编号 domain_id4b9qD02
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily40 — ATPase, nucleotide binding domain
结构域编号 domain_id4b9qD03
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology640 — Actin; Chain A, domain 4
同源超家族 H-superfamily homologous superfamily10 — ATPase, substrate binding domain, subdomain 4
结构域编号 domain_id4b9qD04
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology34 — Substrate Binding Domain Of DNAk; Chain A, domain 1
同源超家族 H-superfamily homologous superfamily10 — Substrate Binding Domain Of DNAk; Chain A, domain 1
结构域编号 domain_id4b9qD05
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1270 — Substrate Binding Domain Of Dnak; Chain:A; Domain 2
同源超家族 H-superfamily homologous superfamily10

8. 引用文献 (1)

9. 文件与曲线 (10)