4eea

Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta1,4-Glc-BETA

Method: X-RAY DIFFRACTION D_max: 97.3 Å Quality: GOOD

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Beta-1,4-galactosyltransferase 1

Homo sapiens

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	其他组合单体蛋白 × 1 其他聚合物 1 PDB 声明：monomeric（1）与蛋白拷贝数一致	链 A； UniProt 126–398	片段：catalytic domain 突变：R337T, C338T, M340H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose × 1 UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 6	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 MM Mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.00 Å R-free 0.240
2	其他组合单体蛋白 × 1 其他聚合物 1 PDB 声明：monomeric（1）与蛋白拷贝数一致	链 B； UniProt 126–398	片段：catalytic domain 突变：R337T, C338T, M340H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose × 1 UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 8	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 MM Mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.00 Å R-free 0.240
3	其他组合单体蛋白 × 1 其他聚合物 1 PDB 声明：monomeric（1）与蛋白拷贝数一致	链 C； UniProt 126–398	片段：catalytic domain 突变：R337T, C338T, M340H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose × 1 UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 6	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 MM Mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.00 Å R-free 0.240
4	其他组合同源多聚体蛋白 × 6 其他聚合物 6 PDB 声明：hexameric（6）与蛋白拷贝数一致	链 A； UniProt 126–398 链 B； UniProt 126–398 链 C； UniProt 126–398	片段：catalytic domain 突变：R337T, C338T, M340H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose × 6 UDP URIDINE-5'-DIPHOSPHATE × 6 MN MANGANESE (II) ION × 6 GOL GLYCEROL × 8 SO4 SULFATE ION × 40	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 MM Mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.00 Å R-free 0.240
5	其他组合同源多聚体蛋白 × 3 其他聚合物 3 PDB 声明：trimeric（3）与蛋白拷贝数一致	链 A； UniProt 126–398 链 B； UniProt 126–398 链 C； UniProt 126–398	片段：catalytic domain 突变：R337T, C338T, M340H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose × 3 UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 4 SO4 SULFATE ION × 20	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 MM Mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.00 Å R-free 0.240

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 4EEA	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
2AE7 Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide 提交 2005-07-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.246
2AE7 Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide 提交 2005-07-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 6 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 5	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.246
2AE7 Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide 提交 2005-07-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.246
2AE7 Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide 提交 2005-07-21	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 4 其他组合同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 125–397（273 aa）片段：Catalytic domain, Residues 126-398 链 B 125–397（273 aa）片段：Catalytic domain, Residues 126-398 链 C 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T 突变：M340H, C338T, R337T 突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 14 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 3 GOL GLYCEROL × 7 DIO 1,4-DIETHYLENE DIOXIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.246
2AEC Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,6-Man-beta-OR 提交 2005-07-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.239
2AEC Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,6-Man-beta-OR 提交 2005-07-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 6 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.239
2AEC Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,6-Man-beta-OR 提交 2005-07-22	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.239
2AES Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,3-Man-beta-OR 提交 2005-07-23	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 3	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.232
2AES Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,3-Man-beta-OR 提交 2005-07-23	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 6 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.232
2AES Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,3-Man-beta-OR 提交 2005-07-23	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 3 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.232
2AGD Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I(M340H-B4Gal-T1) in Complex with GlcNAc-beta1,4-Man-alpha1,3-Man-beta-OR 提交 2005-07-26	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 3	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 1.90 Å R-free 0.228
2AGD Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I(M340H-B4Gal-T1) in Complex with GlcNAc-beta1,4-Man-alpha1,3-Man-beta-OR 提交 2005-07-26	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 7 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 1.90 Å R-free 0.228
2AGD Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I(M340H-B4Gal-T1) in Complex with GlcNAc-beta1,4-Man-alpha1,3-Man-beta-OR 提交 2005-07-26	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 5 UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 1.90 Å R-free 0.228
2AH9 Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with Chitotriose 提交 2005-07-27	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 4 DIO 1,4-DIETHYLENE DIOXIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.224
2AH9 Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with Chitotriose 提交 2005-07-27	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 4 SO4 SULFATE ION × 7	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.224
2AH9 Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with Chitotriose 提交 2005-07-27	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 5 DIO 1,4-DIETHYLENE DIOXIDE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.224
2AH9 Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with Chitotriose 提交 2005-07-27	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 4 其他组合同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 125–397（273 aa）片段：Catalytic domain, Residues 126-398 链 B 125–397（273 aa）片段：Catalytic domain, Residues 126-398 链 C 125–397（273 aa）片段：Catalytic domain, Residues 126-398	突变：M340H, C338T, R337T 突变：M340H, C338T, R337T 突变：M340H, C338T, R337T	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 7 SO4 SULFATE ION × 16 DIO 1,4-DIETHYLENE DIOXIDE × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；mes, ammonium sulfate, dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.00 Å R-free 0.224
2FY7 Crystal structure of the catalytic domain of the human beta1,4-galactosyltransferase mutant M339H in apo form 提交 2006-02-07	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 125–397（273 aa）片段：catalytic domain, residues 125-397	突变：R337T, C338T, M340H	PGE TRIETHYLENE GLYCOL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 5.6；298 K；50 mM sodium citrate buffer, 6% PEG 4000, pH 5.6, VAPOR DIFFUSION, temperature 298K	分辨率 1.70 Å R-free 0.222
2FYA Crystal structure of the catalytic domain of the human beta1, 4-galactosyltransferase mutant M339H complex with manganese 提交 2006-02-07	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 125–397（273 aa）片段：catalytic domain, residues 125-397	突变：R337T, C338T, M340H	MN MANGANESE (II) ION × 1 PGE TRIETHYLENE GLYCOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 5.6；298 K；10 mg/ml protein solution, 5 mM manganese chloride, 50 mM sodium citrate buffer, 6% PEG 4000, pH 5.6, VAPOR DIFFUSION, temperature 298K	分辨率 1.90 Å R-free 0.226
2FYB Crystal structure of the catalytic domain of the human beta1,4-galactosyltransferase mutant M339H in complex with Mn and UDP-galactose in open conformation 提交 2006-02-07	构建体不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 125–397（273 aa）片段：catalytic domain, residues 125-397	突变：R337T, C338T, M340H	MN MANGANESE (II) ION × 1 UDP URIDINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 5.6；298 K；10 mg/ml protein solution, 5mM manganese chloride, 5mM UDP-galactose, 50 mM sodium citrate, 6% PEG 4000, pH 5.6, VAPOR DIFFUSION, temperature 298K	分辨率 1.90 Å R-free 0.216
3EE5 Crystal structure of human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in complex with GLCNAC-Beta1,3-Gal-Beta-Naphthalenemethanol 提交 2008-09-04	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 126–398（273 aa）片段：Catalytic domain of Beta-1,4-Galactosyltransferase	突变：d126, M340H, C338T, R337T	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 2NA naphthalen-2-ylmethanol × 1 SO4 SULFATE ION × 5 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray结晶条件 EVAPORATION；pH 6.5；298 K；MES, AMMONIUM SULFATE, DIOXANE, pH 6.5, EVAPORATION, temperature 298K	分辨率 2.20 Å R-free 0.240
3EE5 Crystal structure of human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in complex with GLCNAC-Beta1,3-Gal-Beta-Naphthalenemethanol 提交 2008-09-04	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 126–398（273 aa）片段：Catalytic domain of Beta-1,4-Galactosyltransferase	突变：d126, M340H, C338T, R337T	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 2NA naphthalen-2-ylmethanol × 1 SO4 SULFATE ION × 7 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 4	X-RAY DIFFRACTION X-ray结晶条件 EVAPORATION；pH 6.5；298 K；MES, AMMONIUM SULFATE, DIOXANE, pH 6.5, EVAPORATION, temperature 298K	分辨率 2.20 Å R-free 0.240
3EE5 Crystal structure of human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in complex with GLCNAC-Beta1,3-Gal-Beta-Naphthalenemethanol 提交 2008-09-04	构建体不同突变/修饰不同配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 126–398（273 aa）片段：Catalytic domain of Beta-1,4-Galactosyltransferase	突变：d126, M340H, C338T, R337T	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 2NA naphthalen-2-ylmethanol × 1 SO4 SULFATE ION × 2 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1	X-RAY DIFFRACTION X-ray结晶条件 EVAPORATION；pH 6.5；298 K；MES, AMMONIUM SULFATE, DIOXANE, pH 6.5, EVAPORATION, temperature 298K	分辨率 2.20 Å R-free 0.240
4EE3 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 8 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.240
4EE3 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 6 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.240
4EE3 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.240
4EE3 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 4 其他组合同源多聚体；蛋白 × 6 PDB 声明：hexameric	链 A 126–398（273 aa）片段：Catalytic domain 链 B 126–398（273 aa）片段：Catalytic domain 链 C 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H 突变：R337T, C338T, M340H 突变：R337T, C338T, M340H	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 6 MN MANGANESE (II) ION × 6 SO4 SULFATE ION × 36 GOL GLYCEROL × 6	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.240
4EE3 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 5 其他组合同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 126–398（273 aa）片段：Catalytic domain 链 B 126–398（273 aa）片段：Catalytic domain 链 C 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H 突变：R337T, C338T, M340H 突变：R337T, C338T, M340H	UDH 6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 18 GOL GLYCEROL × 3	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.240
4EE4 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 7 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 1.95 Å R-free 0.232
4EE4 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 11 GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 1.95 Å R-free 0.232
4EE4 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 1.95 Å R-free 0.232
4EE4 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 4 其他组合同源多聚体；蛋白 × 6 PDB 声明：hexameric	链 A 126–398（273 aa）片段：Catalytic domain 链 B 126–398（273 aa）片段：Catalytic domain 链 C 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H 突变：R337T, C338T, M340H 突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 6 MN MANGANESE (II) ION × 6 SO4 SULFATE ION × 44 GOL GLYCEROL × 8	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 1.95 Å R-free 0.232
4EE4 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 5 其他组合同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 126–398（273 aa）片段：Catalytic domain 链 B 126–398（273 aa）片段：Catalytic domain 链 C 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H 突变：R337T, C338T, M340H 突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 22 GOL GLYCEROL × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 1.95 Å R-free 0.232
4EE5 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.250
4EE5 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 5	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.250
4EE5 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.250
4EE5 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 4 其他组合同源多聚体；蛋白 × 6 PDB 声明：hexameric	链 A 126–398（273 aa）片段：catalytic domain 链 B 126–398（273 aa）片段：catalytic domain 链 C 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H 突变：R337T, C338T, M340H 突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 6 MN MANGANESE (II) ION × 6 GOL GLYCEROL × 8 SO4 SULFATE ION × 22	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.250
4EE5 Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 5 其他组合同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 126–398（273 aa）片段：catalytic domain 链 B 126–398（273 aa）片段：catalytic domain 链 C 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H 突变：R337T, C338T, M340H 突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 4 SO4 SULFATE ION × 11	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.250
4EEG Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 3	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M Ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.244
4EEG Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 5	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M Ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.244
4EEG Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 GOL GLYCEROL × 1 SO4 SULFATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M Ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.244
4EEG Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 4 其他组合同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 126–398（273 aa）片段：catalytic domain 链 B 126–398（273 aa）片段：catalytic domain 链 C 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H 突变：R337T, C338T, M340H 突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 GOL GLYCEROL × 4 SO4 SULFATE ION × 10	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M Ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.244
4EEM Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-MAN-ALPHA-methyl 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.236
4EEM Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-MAN-ALPHA-methyl 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 7 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.236
4EEM Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-MAN-ALPHA-methyl 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 3 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.236
4EEM Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-MAN-ALPHA-methyl 提交 2012-03-28	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 4 其他组合同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 126–398（273 aa）片段：Catalytic domain 链 B 126–398（273 aa）片段：Catalytic domain 链 C 126–398（273 aa）片段：Catalytic domain	突变：R337T, C338T, M340H 突变：R337T, C338T, M340H 突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 14 GOL GLYCEROL × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM Mes buffer, 1.6 M ammonium sulfate, 2% Dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.236
4EEO Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-GlcNAc-ALPHA-benzyl 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.255
4EEO Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-GlcNAc-ALPHA-benzyl 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 2 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 B 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 5 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.255
4EEO Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-GlcNAc-ALPHA-benzyl 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 3 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 C 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 1 MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.255
4EEO Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-GlcNAc-ALPHA-benzyl 提交 2012-03-28	配体/离子不同实验环境不同结构质量不同	Assembly 4 其他组合同源多聚体；蛋白 × 3 PDB 声明：trimeric	链 A 126–398（273 aa）片段：catalytic domain 链 B 126–398（273 aa）片段：catalytic domain 链 C 126–398（273 aa）片段：catalytic domain	突变：R337T, C338T, M340H 突变：R337T, C338T, M340H 突变：R337T, C338T, M340H	UDP URIDINE-5'-DIPHOSPHATE × 3 MN MANGANESE (II) ION × 3 SO4 SULFATE ION × 11 GOL GLYCEROL × 3	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；100 mM mes buffer, 1.6 M ammonium sulfate, 2% dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.255
4L41 Human Lactose synthase: A 2:1 complex between human alpha-lactalbumin and human beta1,4-galactosyltransferase 提交 2013-06-07	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 C 126–398（273 aa）片段：UNP residues 126-398	突变：R337T, C338T	CA CALCIUM ION × 2 SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；100 mM mes buffer pH 7.0, 12% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K	分辨率 2.70 Å R-free 0.237
6FWT Crystal structure of human wild type beta-1,4-galactosyltransferase-1 (B4GalT1) in apo-open monomeric form 提交 2018-03-07	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 122–398（277 aa）	未记录	GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；293 K；0.2 M Potassium Nitrate, 20% (w/v) PEG 3350	分辨率 1.84 Å R-free 0.218
6FWU Crystal structure of human wild type beta-1,4-galactosyltransferase-1 (B4GalT1) in apo-closed dimeric form 提交 2018-03-07	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 126–398（273 aa）链 B 126–398（273 aa）	未记录	NO3 NITRATE ION × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Potassium Nitrate, 20% (w/v) PEG 3350	分辨率 2.35 Å R-free 0.256

共 18 个其他 PDB 条目、53 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	B4GT1_HUMAN
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 15–287； UniProt 126–398 作者链 B； PDB构建体 15–287； UniProt 126–398 作者链 C； PDB构建体 15–287； UniProt 126–398

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	4eea
沉积日期 deposition_date	2012-03-28
结构标题 title	Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta1,4-Glc-BETA
关键词 keywords	enzyme-carbohydrate complex, GT-A fold, glycosyltransferase, UDP-Galactose, Transferase; TRANSFERASE
实验方法 method	X-RAY DIFFRACTION

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	32.43 Å
回转半径 Rg (电子) rg_electron	31.68 Å
零角强度 I(0) i0	165535000.00
分子量 molecular_weight	99560.0 kDa
排除体积 excluded_volume	122910 Å³
包络体积 envelope_volume	147960 Å³
水化壳体积 shell_volume	39205 Å³
包络直径 envelope_diameter	97.6 Å
壳层 Rg shell_rg	38.50 Å
包络 Rg envelope_rg	31.45 Å
形状 Rg shape_rg	31.70 Å
总 Rg total_rg	32.12 Å
总原子数 total_atoms	6967
残基数 n_residues	819
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	97.3 Å
Rg (实空间) rg_real	32.35 Å
Rg 误差 (实空间) rg_real_error	0.55 Å
I(0) (实空间) i0_real	1.6550e+08
I(0) 误差 (实空间) i0_real_error	2.4400e+06
Rg (倒空间) rg_reciprocal	32.39 Å
I(0) (倒空间) i0_reciprocal	165500000.0000
解质量估计 total_estimate	0.8983
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	31.8 Å
偏度 Skewness skewness	0.153
峰度 Kurtosis kurtosis	-0.790
角度范围 angular_range	— – 0.2450 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	52290000.0000
实空间数据点数 n_real_points	50
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.970; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.994; Smooth: 0.768

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (7)

实体 1 Entity 1 polymer

描述 description	Beta-1,4-galactosyltransferase 1
分子量 formula_weight	32773.2 kDa
EC 编号 ec_number	2.4.1.-, 2.4.1.22, 2.4.1.90, 2.4.1.38
突变 mutation	R337T, C338T, M340H
来源方法 src_method	man
拷贝数 entity_count	3

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B,C
原子数 atom_count	7740

ASMTGGQQMGRGSASLPACPEESPLLVGPMLIEFNMPVDLELVAKQNPNVKMGGRYAPRDCVSPHKVAIIIPFRNRQEHLKYWLYYLHPVLQRQQLDYGIYVINQAGDTIFNRAKLLNVGFQEALKDYDYTCFVFSDVDLIPMNDHNAYRCFSQPRHISVAMDKFGFSLPYVQYFGGVSALSKQQFLTINGFPNNYWGWGGEDDDIFNRLVFRGMSISRPNAVVGTTRHIRHSRDKKNEPNPQRFDRIAHTKETMLSDGLNSLTYQVLDVQRYPLYTQITVDIGTPS

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

实体 2 Entity 2 branched

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose
分子量 formula_weight	545.5 kDa
来源方法 src_method	man
拷贝数 entity_count	3

实体 3 Entity 3 non-polymer

描述 description	URIDINE-5'-DIPHOSPHATE
分子量 formula_weight	404.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	3

实体 4 Entity 4 non-polymer

描述 description	MANGANESE (II) ION
分子量 formula_weight	54.9 kDa
来源方法 src_method	syn
拷贝数 entity_count	3

实体 5 Entity 5 non-polymer

描述 description	GLYCEROL
分子量 formula_weight	92.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

实体 6 Entity 6 non-polymer

描述 description	SULFATE ION
分子量 formula_weight	96.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	20

实体 7 Entity 7 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	724

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7. 折叠分类 (SCOP + CATH) 6 domains

SCOP 2.08 (3 domains)

结构域编号 domain_id	d4eeaa_
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.68 — Nucleotide-diphospho-sugar transferases
超家族 Superfamily superfamily	c.68.1 — Nucleotide-diphospho-sugar transferases
家族 Family family	c.68.1.2 — beta 1,4 galactosyltransferase (b4GalT1)

结构域编号 domain_id	d4eeab_
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.68 — Nucleotide-diphospho-sugar transferases
超家族 Superfamily superfamily	c.68.1 — Nucleotide-diphospho-sugar transferases
家族 Family family	c.68.1.2 — beta 1,4 galactosyltransferase (b4GalT1)

结构域编号 domain_id	d4eeac_
类 Class class	c — Alpha and beta proteins (a/b)
折叠类型 Fold fold	c.68 — Nucleotide-diphospho-sugar transferases
超家族 Superfamily superfamily	c.68.1 — Nucleotide-diphospho-sugar transferases
家族 Family family	c.68.1.2 — beta 1,4 galactosyltransferase (b4GalT1)

CATH v4.4 (3 domains)

结构域编号 domain_id	4eeaA00
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	550 — Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
同源超家族 H-superfamily homologous superfamily	10 — Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

结构域编号 domain_id	4eeaB00
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	550 — Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
同源超家族 H-superfamily homologous superfamily	10 — Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

结构域编号 domain_id	4eeaC00
类 Class class	3 — Alpha Beta
架构 Architecture architecture	90 — Alpha-Beta Complex
拓扑 Topology topology	550 — Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
同源超家族 H-superfamily homologous superfamily	10 — Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

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8. 引用文献 (1)

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晶胞边长 a length_a	107.322 Å
晶胞边长 b length_b	196.204 Å
晶胞边长 c length_c	143.594 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	24

空间群 space_group	C 2 2 21
空间群编号 int_tables_number	20
晶系 crystal_system	—

分辨率 resolution	2.000 Å
最低分辨率 resolution_low	40.500 Å
R 因子 r_factor	0.2021
R_work r_work	0.2000
R_free r_free	0.2398

fit	/app/data/saxs_computation/ee/4eea/4eea.fit
pddf	/app/data/pr_computation/ee/4eea/4eea.pr.out
profile	/app/data/saxs_computation/ee/4eea/4eea.dat