4ejq

Crystal structure of KIF1A C-CC1-FHA

Method: X-RAY DIFFRACTION D_max: 127.3 Å Quality: GOOD

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Kinesin-like protein KIF1A

Homo sapiens

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白同源多聚体同源多聚体蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 A； UniProt 458–607 链 B； UniProt 458–607	片段：C-CC1-FHA, UNP residues 458-607	无其他共同聚合物	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 7.2；289 K；0.2 M calcium acetate hydrate, 18% (w/v) PEG 3000, 0.1 M Bis-Tris, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K	分辨率 1.89 Å R-free 0.231
2	蛋白同源多聚体同源多聚体蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 C； UniProt 458–607 链 D； UniProt 458–607	片段：C-CC1-FHA, UNP residues 458-607	无其他共同聚合物	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 7.2；289 K；0.2 M calcium acetate hydrate, 18% (w/v) PEG 3000, 0.1 M Bis-Tris, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K	分辨率 1.89 Å R-free 0.231
3	蛋白同源多聚体同源多聚体蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 E； UniProt 458–607 链 F； UniProt 458–607	片段：C-CC1-FHA, UNP residues 458-607	无其他共同聚合物	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 7.2；289 K；0.2 M calcium acetate hydrate, 18% (w/v) PEG 3000, 0.1 M Bis-Tris, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K	分辨率 1.89 Å R-free 0.231
4	蛋白同源多聚体同源多聚体蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 G； UniProt 458–607 链 H； UniProt 458–607	片段：C-CC1-FHA, UNP residues 458-607	无其他共同聚合物	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, HANGING DROP；pH 7.2；289 K；0.2 M calcium acetate hydrate, 18% (w/v) PEG 3000, 0.1 M Bis-Tris, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K	分辨率 1.89 Å R-free 0.231

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 4EJQ	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
4EGX Crystal structure of KIF1A CC1-FHA tandem 提交 2012-04-02	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 430–607（178 aa）片段：CC1-FHA tandem, UNP residues 430-607 链 B 430–607（178 aa）片段：CC1-FHA tandem, UNP residues 430-607	未记录	PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 5.8；289 K；18% (w/v) PEG 5000 MME, 8% (v/v) Tacsimate (pH 6.0), 0.1 M Bis-Tris, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K	分辨率 2.51 Å R-free 0.283
4EGX Crystal structure of KIF1A CC1-FHA tandem 提交 2012-04-02	构建体不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 C 430–607（178 aa）片段：CC1-FHA tandem, UNP residues 430-607 链 D 430–607（178 aa）片段：CC1-FHA tandem, UNP residues 430-607	未记录	PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 5.8；289 K；18% (w/v) PEG 5000 MME, 8% (v/v) Tacsimate (pH 6.0), 0.1 M Bis-Tris, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K	分辨率 2.51 Å R-free 0.283
4UXO Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins 提交 2014-08-27	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 C 1–361（361 aa）	未记录	ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 10 U/ML APYRASE；pH 6.8；20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 10 U/ML APYRASE cryo-EM玻璃化条件冷冻剂 ETHANE；LIQUID ETHANE	分辨率 6.30 Å
4UXP Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins 提交 2014-08-27	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 C 1–361（361 aa）	未记录	ZN ZINC ION × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 10 U/ML APYRASE；pH 6.8；20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 10 U/ML APYRASE cryo-EM玻璃化条件冷冻剂 ETHANE；LIQUID ETHANE	分辨率 6.30 Å
4UXR Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins 提交 2014-08-27	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 C 1–361（361 aa）	未记录	ZN ZINC ION × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ALF TETRAFLUOROALUMINATE ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 7.00 Å
4UXS Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins 提交 2014-08-27	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 C 1–361（361 aa）	未记录	ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 2MM ADP；pH 6.8；20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 2MM ADP cryo-EM玻璃化条件冷冻剂 ETHANE；LIQUID ETHANE	分辨率 7.00 Å
8UTN KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule (class T23L1) 提交 2023-10-31	构建体不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 7 PDB 声明：heptameric	链 K 1–393（393 aa）片段：residues 1-393 链 N 1–393（393 aa）片段：residues 1-393	未记录	MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.10 Å
8UTO KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule - class T2L1 提交 2023-10-31	构建体不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 7 PDB 声明：heptameric	链 K 1–393（393 aa）片段：residues 1-393 链 N 1–393（393 aa）片段：residues 1-393	未记录	MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.20 Å
8UTP KIF1A[1-393] - AMP-PNP two-heads-bound state in complex with a microtubule - class T3L1 提交 2023-10-31	构建体不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 7 PDB 声明：heptameric	链 K 1–393（393 aa）片段：residues 1-393 链 N 1–393（393 aa）片段：residues 1-393	未记录	MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.20 Å
8UTQ KIF1A[1-393] AMP-PNP bound one-head-bound state in complex with a microtubule - class T1L02* 提交 2023-10-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 5 PDB 声明：pentameric	链 K 1–393（393 aa）片段：residues 1-393	未记录	MG MAGNESIUM ION × 3 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.10 Å
8UTR KIF1A[1-393] ADP bound in complex with a microtubule 提交 2023-10-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 K 1–393（393 aa）片段：residues 1-393	未记录	ADP ADENOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.30 Å
8UTS KIF1A[1-393] APO in complex with a microtubule 提交 2023-10-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 K 1–393（393 aa）片段：residues 1-393	未记录	GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.70 Å
8UTT KIF1A[1-393] P305L mutant AMP-PNP bound two-heads-bound state in complex with a microtubule 提交 2023-10-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 7 PDB 声明：heptameric	链 K 1–393（393 aa）片段：residues 1-393 链 N 1–393（393 aa）片段：residues 1-393	突变：P305L 突变：P305L	MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.10 Å
8UTU KIF1A[1-393] P305L mutant AMP-PNP bound one and two heads bound states merged, in complex with a microtubule 提交 2023-10-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 K 1–393（393 aa）片段：residues 1-393	突变：P305L	MG MAGNESIUM ION × 3 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.00 Å
8UTV KIF1A[1-393] P305L mutant ADP bound in complex with a microtubule 提交 2023-10-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 4 PDB 声明：tetrameric	链 K 1–393（393 aa）片段：residues 1-393	突变：P305L	GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.00 Å
8UTW KIF1A[1-393] P305L mutant APO in complex with a microtubule 提交 2023-10-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 K 1–393（393 aa）片段：residues 1-393	突变：P305L	GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.40 Å
8UTY KIF1A[1-393] P364L mutant AMP-PNP bound two-heads-bound state in complex with a microtubule 提交 2023-10-31	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 7 PDB 声明：heptameric	链 K 1–393（393 aa）片段：residues 1-393 链 N 1–393（393 aa）片段：residues 1-393	突变：P364L 突变：P364L	GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.30 Å
9YA5 KIF1A R350G bound to microtubules in two-heads-bound state with AMP-PNP 提交 2025-09-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 K 1–393（393 aa）链 N 1–393（393 aa）	突变：R350G 突变：R350G	GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8；BRB80 buffer composed of 80 mM PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 20uM KIF1A-R350G in BRB80 supplemented with 20uM paclitaxel and 5mM AMP-PNP was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.	分辨率 2.95 Å
9YA7 KIF1A R350G bound to microtubules in the apo state 提交 2025-09-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 K 1–393（393 aa）	突变：R350	GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8；BRB80 buffer composed of 80 mM PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 20uM KIF1A-R350G in BRB80 supplemented with 20uM paclitaxel and 0.005 units per uL apyrase was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.	分辨率 3.29 Å
9YAB KIF1A R350W bound to microtubules in the apo state 提交 2025-09-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 K 1–393（393 aa）	突变：R350W	GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8；BRB80 buffer composed of 80 mM PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 17.5uM KIF1A-R350W in BRB80 supplemented with 20uM paclitaxel and 0.005 units per uL apyrase was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.	分辨率 3.21 Å
9YAI KIF1A R350W bound to microtubules in two-heads-bound state with AMP-PNP 提交 2025-09-16	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 6 PDB 声明：hexameric	链 K 1–393（393 aa）链 N 1–393（393 aa）	突变：R350W 突变：R350W	MG MAGNESIUM ION × 3 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 6.8；BRB80 buffer composed of 80 mM PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8 cryo-EM玻璃化条件冷冻剂 ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 17.5uM KIF1A-R350W in BRB80 supplemented with 20uM paclitaxel and 5mM AMP-PNP was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.	分辨率 3.12 Å

共 20 个其他 PDB 条目、21 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	KIF1A_HUMAN
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 5–154； UniProt 458–607 作者链 B； PDB构建体 5–154； UniProt 458–607 作者链 C； PDB构建体 5–154； UniProt 458–607 作者链 D； PDB构建体 5–154； UniProt 458–607 作者链 E； PDB构建体 5–154； UniProt 458–607 作者链 F； PDB构建体 5–154； UniProt 458–607 作者链 G； PDB构建体 5–154； UniProt 458–607 作者链 H； PDB构建体 5–154； UniProt 458–607

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	4ejq
沉积日期 deposition_date	2012-04-06
结构标题 title	Crystal structure of KIF1A C-CC1-FHA
关键词 keywords	HOMODIMER, FHA DOMAIN, TRANSPORT PROTEIN; TRANSPORT PROTEIN
实验方法 method	X-RAY DIFFRACTION

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	40.75 Å
回转半径 Rg (电子) rg_electron	40.43 Å
零角强度 I(0) i0	300846000.00
分子量 molecular_weight	134120.0 kDa
排除体积 excluded_volume	165270 Å³
包络体积 envelope_volume	240610 Å³
水化壳体积 shell_volume	51067 Å³
包络直径 envelope_diameter	131.9 Å
壳层 Rg shell_rg	44.37 Å
包络 Rg envelope_rg	39.59 Å
形状 Rg shape_rg	40.43 Å
总 Rg total_rg	40.62 Å
总原子数 total_atoms	9396
残基数 n_residues	1194
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	127.3 Å
Rg (实空间) rg_real	40.77 Å
Rg 误差 (实空间) rg_real_error	1.01 Å
I(0) (实空间) i0_real	3.0080e+08
I(0) 误差 (实空间) i0_real_error	5.0460e+06
Rg (倒空间) rg_reciprocal	40.75 Å
I(0) (倒空间) i0_reciprocal	300800000.0000
解质量估计 total_estimate	0.8768
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	1
主峰位置 r_peak_primary	39.2 Å
偏度 Skewness skewness	0.260
峰度 Kurtosis kurtosis	-0.702
角度范围 angular_range	— – 0.1950 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	35640000.0000
实空间数据点数 n_real_points	40
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.959; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.972; Smooth: 0.547

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (2)

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7. 折叠分类 (SCOP + CATH) 16 domains

CATH v4.4 (16 domains)

结构域编号 domain_id	4ejqA01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	2530 —

结构域编号 domain_id	4ejqA02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	200 — Tumour Suppressor Smad4
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	4ejqB01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	2530 —

结构域编号 domain_id	4ejqB02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	200 — Tumour Suppressor Smad4
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	4ejqC01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	2530 —

结构域编号 domain_id	4ejqC02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	200 — Tumour Suppressor Smad4
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	4ejqD01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	2530 —

结构域编号 domain_id	4ejqD02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	200 — Tumour Suppressor Smad4
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	4ejqE01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	2530 —

结构域编号 domain_id	4ejqE02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	200 — Tumour Suppressor Smad4
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	4ejqF01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	2530 —

结构域编号 domain_id	4ejqF02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	200 — Tumour Suppressor Smad4
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	4ejqG01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	2530 —

结构域编号 domain_id	4ejqG02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	200 — Tumour Suppressor Smad4
同源超家族 H-superfamily homologous superfamily	20 —

结构域编号 domain_id	4ejqH01
类 Class class	6 — Special
架构 Architecture architecture	10 — Helix non-globular
拓扑 Topology topology	250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily	2530 —

结构域编号 domain_id	4ejqH02
类 Class class	2 — Mainly Beta
架构 Architecture architecture	60 — Sandwich
拓扑 Topology topology	200 — Tumour Suppressor Smad4
同源超家族 H-superfamily homologous superfamily	20 —

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8. 引用文献 (1)

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晶胞边长 a length_a	42.945 Å
晶胞边长 b length_b	57.844 Å
晶胞边长 c length_c	110.394 Å
晶胞夹角 α angle_alpha	89.98 °
晶胞夹角 β angle_beta	89.96 °
晶胞夹角 γ angle_gamma	90.63 °
晶胞分子数 Z z_pdb	8

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	1.893 Å
最低分辨率 resolution_low	33.906 Å
R 因子 r_factor	0.1883
R_work r_work	0.1872
R_free r_free	0.2313

描述 description	Kinesin-like protein KIF1A
分子量 formula_weight	17378.6 kDa
来源方法 src_method	man
拷贝数 entity_count	8

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B,C,D,E,F,G,H
原子数 atom_count	10054

Crystal structure of KIF1A C-CC1-FHA

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Kinesin-like protein KIF1A

当前结构中的状态

数据库中的同蛋白其他状态

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

2. 结构基本信息 2. Structure Basics

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

5. 晶体学与实验 5. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

6. 实体与聚合物信息 Entities & Polymers (2)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 water

7. 折叠分类 (SCOP + CATH) 16 domains

CATH v4.4 (16 domains)

8. 引用文献 (1)

9. 文件与曲线 (10)

Files (7)

Curves (3)

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	658

fit	/app/data/saxs_computation/ej/4ejq/4ejq.fit
pddf	/app/data/pr_computation/ej/4ejq/4ejq.pr.out
profile	/app/data/saxs_computation/ej/4ejq/4ejq.dat