Beta-lactamase TEM
Escherichia coli
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 24–286 | Mutation：A42G, N52A, I84V, R120G, M182T, L201A, T265M | CA CALCIUM ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K | Resolution 2.68 Å R-free 0.265 |
| 2 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain B； UniProt 24–286 | Mutation：A42G, N52A, I84V, R120G, M182T, L201A, T265M | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K | Resolution 2.68 Å R-free 0.265 |
| 3 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain C； UniProt 24–286 | Mutation：A42G, N52A, I84V, R120G, M182T, L201A, T265M | CA CALCIUM ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K | Resolution 2.68 Å R-free 0.265 |
| 4 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain D； UniProt 24–286 | Mutation：A42G, N52A, I84V, R120G, M182T, L201A, T265M | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K | Resolution 2.68 Å R-free 0.265 |
| 5 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain E； UniProt 24–286 | Mutation：A42G, N52A, I84V, R120G, M182T, L201A, T265M | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 6.2；293 K；9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K | Resolution 2.68 Å R-free 0.265 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 4IBX | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AXB TEM-1 BETA-LACTAMASE FROM ESCHERICHIA COLI INHIBITED WITH AN ACYLATION TRANSITION STATE ANALOG Deposited 1997-10-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：VARIANT V84I, A184V | FOS [[N-(BENZYLOXYCARBONYL)AMINO]METHYL]PHOSPHATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.8；BUFFER NA/K-PHOSPHATE 20MM PH 7.8 AMMONIUM SULFATE 45% SATURATION (4 DEGREES) ACETONE 4% (V/V) PROTEIN 14.5 MG/ML
|
Resolution 2.00 Å R-free 0.206 |
| 1BT5 CRYSTAL STRUCTURE OF THE IMIPENEM INHIBITED TEM-1 BETA-LACTAMASE FROM ESCHERICHIA COLI Deposited 1998-09-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：V82I, A182V | SO4 SULFATE ION × 8 IM2 (5R)-5-[(1S,2R)-1-formyl-2-hydroxypropyl]-3-[(2-{[(E)-iminomethyl]amino}ethyl)sulfanyl]-4,5-dihydro-1H-pyrrole-2-carbox ylic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.8；pH 7.8
|
Resolution 1.80 Å R-free 0.238 |
| 1BTL CRYSTAL STRUCTURE OF ESCHERICHIA COLI TEM1 BETA-LACTAMASE AT 1.8 ANGSTROMS RESOLUTION Deposited 1993-11-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.80 Å |
| 1CK3 N276D MUTANT OF ESCHERICHIA COLI TEM-1 BETA-LACTAMASE Deposited 1999-04-27 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：V84I, A184V, N276D | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.8；pH 7.80
|
Resolution 2.28 Å R-free 0.235 |
| 1ERM X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-1-ACETAMIDO-2-(3-CARBOXYPHENYL)ETHANE BORONIC ACID Deposited 2000-04-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | BJI 1(R)-1-ACETAMIDO-2-(3-CARBOXYPHENYL)ETHYL BORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.70 Å |
| 1ERO X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-2-PHENYLACETAMIDO-2-(3-CARBOXYPHENYL)ETHYL BORONIC ACID Deposited 2000-04-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | BJP (1R)-2-PHENYLACETAMIDO-2-(3-CARBOXYPHENYL)ETHYL BORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 2.10 Å |
| 1ERQ X-RAY CRYSTAL STRUCTURE OF TEM-1 BETA LACTAMASE IN COMPLEX WITH A DESIGNED BORONIC ACID INHIBITOR (1R)-1-ACETAMIDO-2-(3-CARBOXY-2-HYDROXYPHENYL)ETHYL BORONIC ACID Deposited 2000-04-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | BJH 1(R)-1-ACETAMIDO-2-(3-CARBOXY-2-HYDROXYPHENYL)ETHYL BORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.90 Å |
| 1ESU S235A MUTANT OF TEM1 BETA-LACTAMASE Deposited 2000-04-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：S235A | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；292 K；Imidazole 0.1M pH7.0, ammonium sulfate 43 to 48%, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.00 Å |
| 1FQG MOLECULAR STRUCTURE OF THE ACYL-ENZYME INTERMEDIATE IN TEM-1 BETA-LACTAMASE Deposited 2000-09-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：GLU166ASN MUTATION | PNM OPEN FORM - PENICILLIN G × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；1.4M sodium potassium phosphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.70 Å |
| 1JTD Crystal structure of beta-lactamase inhibitor protein-II in complex with TEM-1 beta-lactamase Deposited 2001-08-20 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Not recorded | CA CALCIUM ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.4；293 K；PEG8000, calcium acetate, sodium cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.230 |
| 1JTG CRYSTAL STRUCTURE OF TEM-1 BETA-LACTAMASE / BETA-LACTAMASE INHIBITOR PROTEIN COMPLEX Deposited 2001-08-20 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Mutation：I84V,V184A | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.8；pH 8.8
|
Resolution 1.73 Å R-free 0.205 |
| 1JTG CRYSTAL STRUCTURE OF TEM-1 BETA-LACTAMASE / BETA-LACTAMASE INHIBITOR PROTEIN COMPLEX Deposited 2001-08-20 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
24–286（263 aa）
|
Mutation：I84V,V184A | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.8；pH 8.8
|
Resolution 1.73 Å R-free 0.205 |
| 1JVJ CRYSTAL STRUCTURE OF N132A MUTANT OF TEM-1 BETA-LACTAMASE IN COMPLEX WITH A N-FORMIMIDOYL-THIENAMYCINE Deposited 2001-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1
|
Mutation：N132A | K POTASSIUM ION × 5 IM2 (5R)-5-[(1S,2R)-1-formyl-2-hydroxypropyl]-3-[(2-{[(E)-iminomethyl]amino}ethyl)sulfanyl]-4,5-dihydro-1H-pyrrole-2-carbox ylic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium Phospate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.73 Å R-free 0.193 |
| 1JWP Structure of M182T mutant of TEM-1 beta-lactamase Deposited 2001-09-04 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1
|
Mutation：M182T | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.75 Å R-free 0.220 |
| 1JWV Crystal structure of G238A mutant of TEM-1 beta-lactamase in complex with a boronic acid inhibitor (sefb4) Deposited 2001-09-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1
|
Mutation：G238A | K POTASSIUM ION × 5 CB4 PINACOL[[2-AMINO-ALPHA-(1-CARBOXY-1-METHYLETHOXYIMINO)-4-THIAZOLEACETYL]AMINO]METHANEBORONATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.85 Å R-free 0.203 |
| 1JWZ Crystal structure of TEM-64 beta-lactamase in complex with a boronic acid inhibitor (105) Deposited 2001-09-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-64
|
Mutation：E104K/M182T/R164S | 105 N-[5-METHYL-3-O-TOLYL-ISOXAZOLE-4-CARBOXYLIC ACID AMIDE] BORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium phosphate buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.80 Å R-free 0.189 |
| 1LHY Crystal structure of TEM-30 beta-Lactamase at 2.0 Angstrom Deposited 2002-04-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：R241S | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 2.00 Å R-free 0.212 |
| 1LI0 Crystal structure of TEM-32 beta-Lactamase at 1.6 Angstrom Deposited 2002-04-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M69I, M182T | BCT BICARBONATE ION × 1 K POTASSIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.61 Å R-free 0.217 |
| 1LI9 Crystal structure of TEM-34 beta-Lactamase at 1.5 Angstrom Deposited 2002-04-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M69V | PO4 PHOSPHATE ION × 2 K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium buffer, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.52 Å R-free 0.189 |
| 1M40 ULTRA HIGH RESOLUTION CRYSTAL STRUCTURE OF TEM-1 Deposited 2002-07-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | PO4 PHOSPHATE ION × 3 K POTASSIUM ION × 4 CB4 PINACOL[[2-AMINO-ALPHA-(1-CARBOXY-1-METHYLETHOXYIMINO)-4-THIAZOLEACETYL]AMINO]METHANEBORONATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；sodium-potassium buffer, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 0.85 Å R-free 0.112 |
| 1NXY Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (SM2) Deposited 2003-02-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | K POTASSIUM ION × 1 SM2 (1R)-1-(2-THIENYLACETYLAMINO)-1-(3-CARBOXYPHENYL)METHYLBORONIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；sodium-potassium buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.60 Å R-free 0.196 |
| 1NY0 Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (NBF) Deposited 2003-02-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | PO4 PHOSPHATE ION × 1 K POTASSIUM ION × 1 NBF [(2-ETHOXY-1-NAPHTHOYL)AMINO]METHYLBORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；sodium-potassium buffer , pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.75 Å R-free 0.198 |
| 1NYM Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (CXB) Deposited 2003-02-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | K POTASSIUM ION × 3 PO4 PHOSPHATE ION × 2 CXB [(2-AMINO-ALPHA-METHOXYIMINO-4-THIAZOLYLACETYL)AMINO]METHYLBORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；sodium-potassium buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.20 Å R-free 0.148 |
| 1NYY Crystal Structure of the complex between M182T mutant of TEM-1 and a boronic acid inhibitor (105) Deposited 2003-02-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | 105 N-[5-METHYL-3-O-TOLYL-ISOXAZOLE-4-CARBOXYLIC ACID AMIDE] BORONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；sodium-potassium buffer , pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
|
Resolution 1.90 Å R-free 0.230 |
| 1PZO TEM-1 Beta-Lactamase in Complex with a Novel, Core-Disrupting, Allosteric Inhibitor Deposited 2003-07-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | CBT N,N-BIS(4-CHLOROBENZYL)-1H-1,2,3,4-TETRAAZOL-5-AMINE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；potassium phosphate buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.90 Å R-free 0.247 |
| 1PZP TEM-1 Beta-Lactamase in Complex with a Novel, Core-Disrupting, Allosteric Inhibitor Deposited 2003-07-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：R100N | FTA 3-(4-PHENYLAMINO-PHENYLAMINO)-2-(1H-TETRAZOL-5-YL)-ACRYLONITRILE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；295 K；potassium phosphate buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.45 Å R-free 0.245 |
| 1TEM 6 ALPHA HYDROXYMETHYL PENICILLOIC ACID ACYLATED ON THE TEM-1 BETA-LACTAMASE FROM ESCHERICHIA COLI Deposited 1996-05-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | ALP 2-(1-CARBOXY-2-HYDROXY-ETHYL)-5,5-DIMETHYL-THIAZOLIDINE-4-CARBOXYLIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.8；pH 7.8
|
Resolution 1.95 Å R-free 0.202 |
| 1XPB STRUCTURE OF BETA-LACTAMASE TEM1 Deposited 1997-01-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å R-free 0.158 |
| 1XXM The modular architecture of protein-protein binding site Deposited 2004-11-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Mutation：E104A; Y105A | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 5；298 K；LiCl; sodium Acetate; PEG 6000, pH 5., Microbatch, temperature 298K
|
Resolution 1.90 Å R-free 0.247 |
| 1XXM The modular architecture of protein-protein binding site Deposited 2004-11-07 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
24–286（263 aa）
|
Mutation：E104A; Y105A | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 5；298 K；LiCl; sodium Acetate; PEG 6000, pH 5., Microbatch, temperature 298K
|
Resolution 1.90 Å R-free 0.247 |
| 1YT4 Crystal structure of TEM-76 beta-lactamase at 1.4 Angstrom resolution Deposited 2005-02-09 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM
|
Mutation：S130G | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.3；296 K；sodium potassium phosphate, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.40 Å R-free 0.223 |
| 1ZG4 TEM1 beta lactamase Deposited 2005-04-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–286（286 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.55 Å R-free 0.240 |
| 1ZG6 TEM1 beta lactamase mutant S70G Deposited 2005-04-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–286（286 aa）
|
Mutation：S70G | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.275 |
| 2B5R 1B Lactamase / B Lactamase Inhibitor Deposited 2005-09-29 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Mutation：V84I, E104Y, Y105N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.5；298 K；PEG 3350, NH4 Acetate, pH 8.5, Microbatch, temperature 298K
|
Resolution 1.65 Å R-free 0.222 |
| 2B5R 1B Lactamase / B Lactamase Inhibitor Deposited 2005-09-29 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
24–286（263 aa）
|
Mutation：V84I, E104Y, Y105N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.5；298 K；PEG 3350, NH4 Acetate, pH 8.5, Microbatch, temperature 298K
|
Resolution 1.65 Å R-free 0.222 |
| 2V1Z Structure of a TEM-1 beta-lactamase insertant allosterically regulated by kanamycin and anions. Deposited 2007-05-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
25–38（14 aa）
Fragment：RESIDUES 25-38,41-286
Chain A
41–286（246 aa）
Fragment：RESIDUES 25-38,41-286
|
Mutation：YES Mutation：YES | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.2；RESERVOIR: BIS-TRIS 0.1M PH6.2, PEG6000 25%(W/V), NACL 0.3M, NAN3 0.02%(W/V). HANGING DROP: 1UL PROTEIN AND 1 UL RESERVOIR
|
Resolution 1.60 Å R-free 0.206 |
| 2V20 Structure of a TEM-1 beta-lactamase insertant allosterically regulated by kanamycin and anions. Complex with sulfate. Deposited 2007-05-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
25–38（14 aa）
Fragment：RESIDUES 25-38,41-286
Chain A
41–286（246 aa）
Fragment：RESIDUES 25-38,41-286
|
Mutation：YES Mutation：YES | ZN ZINC ION × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；RESERVOIR: MES 0.1M PH 6.5, PEG5000MME 30%(W/V), AMMONIUM SULFATE 0.2M. DROP: 0.5UL PROTEIN SOLUTION AND 0.5 UL RESERVOIR
|
Resolution 1.67 Å R-free 0.223 |
| 3CMZ TEM-1 Class-A beta-lactamase L201P mutant apo structure Deposited 2008-03-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：L201P | PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.7；293 K；1.6M potassium phosphate, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.92 Å R-free 0.242 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 3JYI Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis Deposited 2009-09-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
24–286（263 aa）
|
Mutation：N170G | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG6000, 0.2 M LiCl, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.70 Å R-free 0.260 |
| 4DXB 2.29A structure of the engineered MBP TEM-1 fusion protein RG13 in complex with zinc, P1 space group Deposited 2012-02-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
227–286（60 aa）
Fragment：SEE REMARK 999
Chain A
24–226（203 aa）
Fragment：SEE REMARK 999
|
Not recorded | ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；A 1.0 uL drop was prepared using 0.5 uL protein mixture (13.8 mg/mL RG13, 2.5 mM zinc chloride) and 0.5 uL reservoir solution (0.2 M ammonium acetate, 0.1 M Tris, pH 8.5-9.5, 15-30% PEG3350) and equilibrated over a 1 ml reservoir solution, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
|
Resolution 2.29 Å R-free 0.292 |
| 4DXB 2.29A structure of the engineered MBP TEM-1 fusion protein RG13 in complex with zinc, P1 space group Deposited 2012-02-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
227–286（60 aa）
Fragment：SEE REMARK 999
Chain B
24–226（203 aa）
Fragment：SEE REMARK 999
|
Not recorded | ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；A 1.0 uL drop was prepared using 0.5 uL protein mixture (13.8 mg/mL RG13, 2.5 mM zinc chloride) and 0.5 uL reservoir solution (0.2 M ammonium acetate, 0.1 M Tris, pH 8.5-9.5, 15-30% PEG3350) and equilibrated over a 1 ml reservoir solution, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
|
Resolution 2.29 Å R-free 0.292 |
| 4DXC Crystal structure of the engineered MBP TEM-1 fusion protein RG13, C2 space group Deposited 2012-02-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
227–286（60 aa）
Fragment：SEE REMARK 999
Chain A
24–226（203 aa）
Fragment：SEE REMARK 999
|
Not recorded | ZN ZINC ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；A 1.0 uL drop was prepared using 0.5 uL protein mixture (13.8 mg/mL RG13, 2.5 mM zinc chloride) and 0.5 uL reservoir solution (0.2 M ammonium acetate, 0.1 M Tris, pH 8.5-9.5, 15-30% PEG3350) and equilibrated over a 1 ml reservoir solution, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
|
Resolution 2.30 Å R-free 0.291 |
| 4GKU Crystal structure of beta lactamase in PET-15B Deposited 2012-08-13 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；293 K；0.2M magnesium chloride, 0.1M BIS-Tris, 25% PEG 3350, pH 6.5, VAPOR DIFFUSION, temperature 293K
|
Resolution 1.92 Å R-free 0.197 |
| 4IBR Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying G238S/E104K mutations Deposited 2012-12-09 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：A41G, N51A, R119G, M181T, L200A, T262M, G237S, E103K | CA CALCIUM ION × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；11% (wt/vol) polyethylene glycol (PEG) 8000, 100 mM
MES buffer pH 6.7, 200mM Ca(OAc)2 and 10 M ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.247 |
| 4IBR Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying G238S/E104K mutations Deposited 2012-12-09 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Mutation：A41G, N51A, R119G, M181T, L200A, T262M, G237S, E103K | CA CALCIUM ION × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.7；293 K；11% (wt/vol) polyethylene glycol (PEG) 8000, 100 mM
MES buffer pH 6.7, 200mM Ca(OAc)2 and 10 M ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.247 |
| 4ID4 Crystal structure of chimeric beta-lactamase cTEM-17m Deposited 2012-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–147（124 aa）
Chain A
189–286（98 aa）
|
Not recorded | CL CHLORIDE ION × 5 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；26% PEG 4000, 0.25M magnesium chloride, 0.1M TrisHCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.05 Å R-free 0.138 |
| 4MEZ Crystal structure of M68L/M69T double mutant TEM-1 Deposited 2013-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M66L, M67T | CL CHLORIDE ION × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295.15 K；1.5M ammonium sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K
|
Resolution 2.05 Å R-free 0.248 |
| 4MEZ Crystal structure of M68L/M69T double mutant TEM-1 Deposited 2013-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M66L, M67T | CL CHLORIDE ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295.15 K；1.5M ammonium sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K
|
Resolution 2.05 Å R-free 0.248 |
| 4QY5 Crystal structures of chimeric beta-lactamase cTEM-19m showing different conformations Deposited 2014-07-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–65（42 aa）
Chain A
68–147（80 aa）
Chain A
189–286（98 aa）
|
Not recorded | CL CHLORIDE ION × 4 MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
Resolution 1.50 Å R-free 0.161 |
| 4QY6 Crystal structures of chimeric beta-lactamase cTEM-19m showing different conformations Deposited 2014-07-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–65（42 aa）
Chain A
68–147（80 aa）
Chain A
189–286（98 aa）
|
Not recorded | CL CHLORIDE ION × 6 MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.15 Å R-free 0.139 |
| 4R4R Crystal structure of chimeric beta-lactamase cTEM-19m at 1.2 angstrom resolution Deposited 2014-08-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–65（42 aa）
Chain A
68–147（80 aa）
Chain A
189–286（98 aa）
|
Not recorded | CL CHLORIDE ION × 4 MG MAGNESIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.20 Å R-free 0.138 |
| 4R4S Crystal structure of chimeric beta-lactamase cTEM-19m at 1.1 angstrom resolution Deposited 2014-08-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–65（42 aa）
Chain A
68–147（80 aa）
Chain A
189–286（98 aa）
|
Not recorded | CL CHLORIDE ION × 5 MG MAGNESIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；30% PEG 4000, 0.2M magnesium chloride, 0.1M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.10 Å R-free 0.128 |
| 4RVA A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for deacylation Deposited 2014-11-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1
|
Mutation：W165Y/E166Y/P167G/L201P | BCT BICARBONATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；20% PEG 3350, 0.2M sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.44 Å R-free 0.194 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain G
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX2 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain H
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：W165Y/E166Y/P167G/M182T | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.1M HEPES pH6.5, 30% W/V PEG 6,000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.31 Å R-free 0.268 |
| 4RX3 A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis Deposited 2014-12-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：TEM-1 beta-lactamase
|
Mutation：S70G/W165Y/E166Y/P167G | FLC CITRATE ANION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；298 K；0.24M citrate, 25% w/v PEG 4,000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.39 Å R-free 0.194 |
| 4ZJ1 Crystal Structure of p-acrylamido-phenylalanine modified TEM1 beta-lactamase from Escherichia coli : V216AcrF mutant Deposited 2015-04-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–286（286 aa）
|
Mutation：V216AcrF Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 1 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；295 K；0.1M 2-(N-morpholino) ethanesulfonic acid (MES) pH 6.5, 15% PEG 20.000 and 15% PEG 550MME
|
Resolution 1.54 Å R-free 0.170 |
| 4ZJ2 Crystal Structure of p-acrylamido-phenylalanine modified TEM1 beta-lactamase from Escherichia coli :E166N mutant Deposited 2015-04-28 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–286（286 aa）
|
Mutation：E166N Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；295 K；0.1M 2-(N-morpholino) ethanesulfonic acid (MES) pH 6.5, 15% PEG 20.000 and 15% PEG 550MME
|
Resolution 1.80 Å R-free 0.209 |
| 4ZJ3 Crystal structure of cephalexin bound acyl-enzyme intermediate of Val216AcrF mutant TEM1 beta-lactamase from Escherichia coli: E166N and V216AcrF mutant. Deposited 2015-04-29 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–286（286 aa）
|
Mutation：E166N,V216AcrF Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；295 K；0.1M 2-(N-morpholino) ethanesulfonic acid (MES) pH 6.5, 15% PEG 20.000 and 15% PEG 550MME
|
Resolution 1.70 Å R-free 0.188 |
| 5HVI Crystal structure of TEM1 beta-lactamase Deposited 2016-01-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.64 Å R-free 0.206 |
| 5HVI Crystal structure of TEM1 beta-lactamase Deposited 2016-01-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.64 Å R-free 0.206 |
| 5HVI Crystal structure of TEM1 beta-lactamase Deposited 2016-01-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.64 Å R-free 0.206 |
| 5HVI Crystal structure of TEM1 beta-lactamase Deposited 2016-01-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.64 Å R-free 0.206 |
| 5HW1 Crystal structure of TEM1 beta-lactamase in the presence of 1.2 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.70 Å R-free 0.190 |
| 5HW1 Crystal structure of TEM1 beta-lactamase in the presence of 1.2 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.70 Å R-free 0.190 |
| 5HW1 Crystal structure of TEM1 beta-lactamase in the presence of 1.2 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.70 Å R-free 0.190 |
| 5HW1 Crystal structure of TEM1 beta-lactamase in the presence of 1.2 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T | XE XENON × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.70 Å R-free 0.190 |
| 5HW5 Crystal structure of TEM1 beta-lactamase in the presence of 2.0 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.41 Å R-free 0.221 |
| 5HW5 Crystal structure of TEM1 beta-lactamase in the presence of 2.0 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T | XE XENON × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.41 Å R-free 0.221 |
| 5HW5 Crystal structure of TEM1 beta-lactamase in the presence of 2.0 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.41 Å R-free 0.221 |
| 5HW5 Crystal structure of TEM1 beta-lactamase in the presence of 2.0 MPa xenon Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.41 Å R-free 0.221 |
| 5I52 Crystal structure of TEM1 beta-lactamase mutant I263N Deposited 2016-02-13 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T, I259N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.75 Å R-free 0.218 |
| 5I52 Crystal structure of TEM1 beta-lactamase mutant I263N Deposited 2016-02-13 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T, I259N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.75 Å R-free 0.218 |
| 5I52 Crystal structure of TEM1 beta-lactamase mutant I263N Deposited 2016-02-13 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T, I259N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.75 Å R-free 0.218 |
| 5I52 Crystal structure of TEM1 beta-lactamase mutant I263N Deposited 2016-02-13 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T, I259N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.75 Å R-free 0.218 |
| 5I63 Crystal structure of TEM1 beta-lactamase mutant I263N in the presence of 1.2 MPa xenon Deposited 2016-02-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T, I259N | XE XENON × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.95 Å R-free 0.222 |
| 5I63 Crystal structure of TEM1 beta-lactamase mutant I263N in the presence of 1.2 MPa xenon Deposited 2016-02-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T, I259N | XE XENON × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.95 Å R-free 0.222 |
| 5I63 Crystal structure of TEM1 beta-lactamase mutant I263N in the presence of 1.2 MPa xenon Deposited 2016-02-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T, I259N | XE XENON × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.95 Å R-free 0.222 |
| 5I63 Crystal structure of TEM1 beta-lactamase mutant I263N in the presence of 1.2 MPa xenon Deposited 2016-02-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T, I259N | XE XENON × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；0.2 M sodium formate (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 1.95 Å R-free 0.222 |
| 5IQ8 Crystal structure of TEM1 beta-lactamase mutant A224C/G283C disulfide Deposited 2016-03-10 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T, A222C, G279C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M ammonium tartrate dibasic (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 2.06 Å R-free 0.241 |
| 5IQ8 Crystal structure of TEM1 beta-lactamase mutant A224C/G283C disulfide Deposited 2016-03-10 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T, A222C, G279C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M ammonium tartrate dibasic (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 2.06 Å R-free 0.241 |
| 5IQ8 Crystal structure of TEM1 beta-lactamase mutant A224C/G283C disulfide Deposited 2016-03-10 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T, A222C, G279C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M ammonium tartrate dibasic (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 2.06 Å R-free 0.241 |
| 5IQ8 Crystal structure of TEM1 beta-lactamase mutant A224C/G283C disulfide Deposited 2016-03-10 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T, A222C, G279C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M ammonium tartrate dibasic (pH 7.0), 20% (w/v) PEG 3350
|
Resolution 2.06 Å R-free 0.241 |
| 5KKF Crystal structure of TEM1 beta-lactamase mutant I263L Deposited 2016-06-21 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M180T, I259L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.82 Å R-free 0.227 |
| 5KKF Crystal structure of TEM1 beta-lactamase mutant I263L Deposited 2016-06-21 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M180T, I259L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.82 Å R-free 0.227 |
| 5KKF Crystal structure of TEM1 beta-lactamase mutant I263L Deposited 2016-06-21 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M180T, I259L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.82 Å R-free 0.227 |
| 5KKF Crystal structure of TEM1 beta-lactamase mutant I263L Deposited 2016-06-21 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M180T, I259L | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.82 Å R-free 0.227 |
| 5KPU Crystal structure of TEM1 beta-lactamase mutant I263L in the presence of 1.2 MPa xenon Deposited 2016-07-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T, I263L | XE XENON × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.50 Å R-free 0.193 |
| 5KPU Crystal structure of TEM1 beta-lactamase mutant I263L in the presence of 1.2 MPa xenon Deposited 2016-07-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M182T, I263L | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.50 Å R-free 0.193 |
| 5KPU Crystal structure of TEM1 beta-lactamase mutant I263L in the presence of 1.2 MPa xenon Deposited 2016-07-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M182T, I263L | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.50 Å R-free 0.193 |
| 5KPU Crystal structure of TEM1 beta-lactamase mutant I263L in the presence of 1.2 MPa xenon Deposited 2016-07-05 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M182T, I263L | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；294 K；2% (v/v) tacsimate (pH 6.0), 0.1 M BIS-TRIS (pH 6.5), 20% (w/v) PEG 3350
|
Resolution 1.50 Å R-free 0.193 |
| 5NPO Promiscuous Protein Self-Assembly as a Function of Protein Stability Deposited 2017-04-18 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Not recorded | MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；292 K；7.5% PEG 6000, 0.1M MgCl2 and 0.05M Sodium acetate pH=5.5
|
Resolution 1.95 Å R-free 0.256 |
| 6APA Crystal structure of TEM1 beta-lactamase mutant I263A Deposited 2017-08-17 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.86 Å R-free 0.249 |
| 6APA Crystal structure of TEM1 beta-lactamase mutant I263A Deposited 2017-08-17 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.86 Å R-free 0.249 |
| 6APA Crystal structure of TEM1 beta-lactamase mutant I263A Deposited 2017-08-17 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.86 Å R-free 0.249 |
| 6APA Crystal structure of TEM1 beta-lactamase mutant I263A Deposited 2017-08-17 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.86 Å R-free 0.249 |
| 6AYK Crystal structure of TEM1 beta-lactamase mutant I263A in the presence of 1.2 MPa xenon Deposited 2017-09-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v Tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.44 Å R-free 0.219 |
| 6AYK Crystal structure of TEM1 beta-lactamase mutant I263A in the presence of 1.2 MPa xenon Deposited 2017-09-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v Tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.44 Å R-free 0.219 |
| 6AYK Crystal structure of TEM1 beta-lactamase mutant I263A in the presence of 1.2 MPa xenon Deposited 2017-09-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | XE XENON × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v Tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.44 Å R-free 0.219 |
| 6AYK Crystal structure of TEM1 beta-lactamase mutant I263A in the presence of 1.2 MPa xenon Deposited 2017-09-08 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182T, I263A | XE XENON × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；2% v/v Tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350
|
Resolution 1.44 Å R-free 0.219 |
| 6B2N Crystal structure of TEM-1 beta-lactamase mutant M182N Deposited 2017-09-20 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.2；291 K；0.1 M sodium phosphate dibasic/citric acid, pH 4.2, 0.1 M lithium sulfate, 20% PEG1000
|
Resolution 2.00 Å R-free 0.283 |
| 6B2N Crystal structure of TEM-1 beta-lactamase mutant M182N Deposited 2017-09-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182N | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.2；291 K；0.1 M sodium phosphate dibasic/citric acid, pH 4.2, 0.1 M lithium sulfate, 20% PEG1000
|
Resolution 2.00 Å R-free 0.283 |
| 6B2N Crystal structure of TEM-1 beta-lactamase mutant M182N Deposited 2017-09-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182N | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.2；291 K；0.1 M sodium phosphate dibasic/citric acid, pH 4.2, 0.1 M lithium sulfate, 20% PEG1000
|
Resolution 2.00 Å R-free 0.283 |
| 6B2N Crystal structure of TEM-1 beta-lactamase mutant M182N Deposited 2017-09-20 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
Fragment：UNP residues 24-286
|
Mutation：M182N | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.2；291 K；0.1 M sodium phosphate dibasic/citric acid, pH 4.2, 0.1 M lithium sulfate, 20% PEG1000
|
Resolution 2.00 Å R-free 0.283 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 ACT ACETATE ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QLP Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.3 A resolution Deposited 2021-12-20 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.30 Å R-free 0.217 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
24–286（263 aa）
|
Mutation：V84I | TBE TAZOBACTAM INTERMEDIATE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QNK Structure of beta-lactamase TEM-171 complexed with tazobactam intermediate at 2.5 A resolution Deposited 2021-12-21 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5.
|
Resolution 2.50 Å R-free 0.248 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain AAA
24–286（263 aa）
|
Mutation：V84I | EDO 1,2-ETHANEDIOL × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain BBB
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain CCC
24–286（263 aa）
|
Mutation：V84I | EDO 1,2-ETHANEDIOL × 2 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain DDD
24–286（263 aa）
|
Mutation：V84I | ACT ACETATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain EEE
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 7QOR Structure of beta-lactamase TEM-171 Deposited 2021-12-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain FFF
24–286（263 aa）
|
Mutation：V84I | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.211 |
| 8DDZ TEM-1 beta-lactamase A237Y Deposited 2022-06-19 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T, A237Y | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG 3350, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)
|
Resolution 1.45 Å R-free 0.212 |
| 8DDZ TEM-1 beta-lactamase A237Y Deposited 2022-06-19 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M182T, A237Y | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG 3350, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)
|
Resolution 1.45 Å R-free 0.212 |
| 8DDZ TEM-1 beta-lactamase A237Y Deposited 2022-06-19 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M182T, A237Y | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG 3350, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)
|
Resolution 1.45 Å R-free 0.212 |
| 8DDZ TEM-1 beta-lactamase A237Y Deposited 2022-06-19 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M182T, A237Y | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG 3350, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)
|
Resolution 1.45 Å R-free 0.212 |
| 8DE0 TEM-1 beta-lactamase covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.72 Å R-free 0.198 |
| 8DE0 TEM-1 beta-lactamase covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M182T | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.72 Å R-free 0.198 |
| 8DE0 TEM-1 beta-lactamase covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M182T | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.72 Å R-free 0.198 |
| 8DE0 TEM-1 beta-lactamase covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M182T | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.72 Å R-free 0.198 |
| 8DE1 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.56 Å R-free 0.221 |
| 8DE1 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M182T A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.56 Å R-free 0.221 |
| 8DE1 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M182T A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.56 Å R-free 0.221 |
| 8DE1 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M182T A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 1.56 Å R-free 0.221 |
| 8DE2 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam, a room temperature structure Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
24–286（263 aa）
|
Mutation：M182T, A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 2.45 Å R-free 0.249 |
| 8DE2 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam, a room temperature structure Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
24–286（263 aa）
|
Mutation：M182T, A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 2.45 Å R-free 0.249 |
| 8DE2 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam, a room temperature structure Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
24–286（263 aa）
|
Mutation：M182T, A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 2.45 Å R-free 0.249 |
| 8DE2 TEM-1 beta-lactamase A237Y mutant covalently bound to avibactam, a room temperature structure Deposited 2022-06-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
24–286（263 aa）
|
Mutation：M182T, A237Y | NXL (2S,5R)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；PEG3350, HEPES(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
|
Resolution 2.45 Å R-free 0.249 |
| 9Q0C TEM-1 WT in complex with BLIP E73W Deposited 2025-08-12 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
24–286（263 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.1 M magnesium chloride, 0.1 M sodium acetate pH 4.6, 25% (w/v) PEG 400
|
Resolution 1.78 Å R-free 0.215 |
| 9Q0C TEM-1 WT in complex with BLIP E73W Deposited 2025-08-12 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
24–286（263 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；298 K；0.1 M magnesium chloride, 0.1 M sodium acetate pH 4.6, 25% (w/v) PEG 400
|
Resolution 1.78 Å R-free 0.215 |
72 other PDB entries and 151 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | BLAT_ECOLX |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–263； UniProt 24–286 Author chain B； PDBConstruct 1–263； UniProt 24–286 Author chain C； PDBConstruct 1–263； UniProt 24–286 Author chain D； PDBConstruct 1–263； UniProt 24–286 Author chain E； PDBConstruct 1–263； UniProt 24–286 |
