4lk0

Crystal Structure Analysis of the E.coli holoenzyme/T7 Gp2 complex

Method: X-RAY DIFFRACTION
▼

1. 蛋白身份与相关结构 Protein Identity & Related Structures

DNA-directed RNA polymerase subunit alpha

Escherichia coli

UniProt C9QXI7

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit beta × 1 (C9QV90) ;DNA-directed RNA polymerase subunit beta' ; × 1 (C5A0S8) DNA-directed RNA polymerase subunit omega × 1 (C9QUL2) RNA polymerase sigma factor RpoD × 1 (P00579) Bacterial RNA polymerase inhibitor × 1 (P03704) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致
2 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit beta × 1 (C9QV90) ;DNA-directed RNA polymerase subunit beta' ; × 1 (C5A0S8) DNA-directed RNA polymerase subunit omega × 1 (C9QUL2) RNA polymerase sigma factor RpoD × 1 (P00579) Bacterial RNA polymerase inhibitor × 1 (P03704) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 C9QXI7_ECOD1
Isoform —
PDB实体 1
链与序列区间 作者链 A; PDB构建体 1–234; UniProt 1–234 作者链 B; PDB构建体 1–234; UniProt 1–234 作者链 G; PDB构建体 1–234; UniProt 1–234 作者链 H; PDB构建体 1–234; UniProt 1–234

DNA-directed RNA polymerase subunit beta

Escherichia coli

UniProt C9QV90

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit alpha × 2 (C9QXI7) ;DNA-directed RNA polymerase subunit beta' ; × 1 (C5A0S8) DNA-directed RNA polymerase subunit omega × 1 (C9QUL2) RNA polymerase sigma factor RpoD × 1 (P00579) Bacterial RNA polymerase inhibitor × 1 (P03704) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致
2 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit alpha × 2 (C9QXI7) ;DNA-directed RNA polymerase subunit beta' ; × 1 (C5A0S8) DNA-directed RNA polymerase subunit omega × 1 (C9QUL2) RNA polymerase sigma factor RpoD × 1 (P00579) Bacterial RNA polymerase inhibitor × 1 (P03704) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 C9QV90_ECOD1
Isoform —
PDB实体 2
链与序列区间 作者链 C; PDB构建体 1–1342; UniProt 1–1342 作者链 I; PDB构建体 1–1342; UniProt 1–1342

;DNA-directed RNA polymerase subunit beta' ;

Escherichia coli

UniProt C5A0S8

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit alpha × 2 (C9QXI7) DNA-directed RNA polymerase subunit beta × 1 (C9QV90) DNA-directed RNA polymerase subunit omega × 1 (C9QUL2) RNA polymerase sigma factor RpoD × 1 (P00579) Bacterial RNA polymerase inhibitor × 1 (P03704) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致
2 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit alpha × 2 (C9QXI7) DNA-directed RNA polymerase subunit beta × 1 (C9QV90) DNA-directed RNA polymerase subunit omega × 1 (C9QUL2) RNA polymerase sigma factor RpoD × 1 (P00579) Bacterial RNA polymerase inhibitor × 1 (P03704) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 C5A0S8_ECOBW
Isoform —
PDB实体 3
链与序列区间 作者链 D; PDB构建体 1–1407; UniProt 1–1407 作者链 J; PDB构建体 1–1407; UniProt 1–1407

DNA-directed RNA polymerase subunit omega

Escherichia coli

UniProt C9QUL2

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit alpha × 2 (C9QXI7) DNA-directed RNA polymerase subunit beta × 1 (C9QV90) ;DNA-directed RNA polymerase subunit beta' ; × 1 (C5A0S8) RNA polymerase sigma factor RpoD × 1 (P00579) Bacterial RNA polymerase inhibitor × 1 (P03704) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致
2 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit alpha × 2 (C9QXI7) DNA-directed RNA polymerase subunit beta × 1 (C9QV90) ;DNA-directed RNA polymerase subunit beta' ; × 1 (C5A0S8) RNA polymerase sigma factor RpoD × 1 (P00579) Bacterial RNA polymerase inhibitor × 1 (P03704) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 C9QUL2_ECOD1
Isoform —
PDB实体 4
链与序列区间 作者链 E; PDB构建体 1–91; UniProt 1–91 作者链 K; PDB构建体 1–91; UniProt 1–91

RNA polymerase sigma factor RpoD

Escherichia coli

UniProt P00579

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit alpha × 2 (C9QXI7) DNA-directed RNA polymerase subunit beta × 1 (C9QV90) ;DNA-directed RNA polymerase subunit beta' ; × 1 (C5A0S8) DNA-directed RNA polymerase subunit omega × 1 (C9QUL2) Bacterial RNA polymerase inhibitor × 1 (P03704) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致
2 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit alpha × 2 (C9QXI7) DNA-directed RNA polymerase subunit beta × 1 (C9QV90) ;DNA-directed RNA polymerase subunit beta' ; × 1 (C5A0S8) DNA-directed RNA polymerase subunit omega × 1 (C9QUL2) Bacterial RNA polymerase inhibitor × 1 (P03704) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 RPOD_ECOLI
Isoform —
PDB实体 5
链与序列区间 作者链 F; PDB构建体 1–522; UniProt 92–613 作者链 L; PDB构建体 1–522; UniProt 92–613

Bacterial RNA polymerase inhibitor

Enterobacteria phage T7

UniProt P03704

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit alpha × 2 (C9QXI7) DNA-directed RNA polymerase subunit beta × 1 (C9QV90) ;DNA-directed RNA polymerase subunit beta' ; × 1 (C5A0S8) DNA-directed RNA polymerase subunit omega × 1 (C9QUL2) RNA polymerase sigma factor RpoD × 1 (P00579) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致
2 蛋白异源复合物 异源复合物 蛋白 7 DNA-directed RNA polymerase subunit alpha × 2 (C9QXI7) DNA-directed RNA polymerase subunit beta × 1 (C9QV90) ;DNA-directed RNA polymerase subunit beta' ; × 1 (C5A0S8) DNA-directed RNA polymerase subunit omega × 1 (C9QUL2) RNA polymerase sigma factor RpoD × 1 (P00579) MAGNESIUM ION × 1 ZINC ION × 2 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 VRPI_BPT7
Isoform —
PDB实体 6
链与序列区间 作者链 M; PDB构建体 1–64; UniProt 1–64 作者链 N; PDB构建体 1–64; UniProt 1–64

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

▼

2. 结构基本信息 2. Structure Basics

条目编号 entry_id4lk0
沉积日期 deposition_date2013-07-05
结构标题 titleCrystal Structure Analysis of the E.coli holoenzyme/T7 Gp2 complex
关键词 keywordsDNA directed RNA polymerase, TRANSFERASE; TRANSFERASE
实验方法 methodX-RAY DIFFRACTION
▼

3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

4lk0__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

4lk0__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 个绘图点;横轴和纵轴均采用对数尺度。

4lk0__assembly_1__model_1 | P(r) · Pending

新版 assembly/model P(r) 尚未计算 此处仅保留位置,不展示旧数据 r (Angstrom) P(r)
P(r) 将按相同 assembly/model 分别计算和展示。
Rg(Guinier)52.31 Å
Rg(电子密度)51.91 Å
总 Rg52.11 Å
原子数29363
残基数3739
排除体积523030 ų
最大 q0.500 Å⁻¹
Assembly Model 结构单元 聚集说明 状态 CRYSOL 操作
1 1 4lk0__assembly_1__model_1 heptameric (7) 成功 4.1.3-1-20251215 (887e7ef) 查看 下载
2 1 4lk0__assembly_2__model_1 heptameric (7) 成功 4.1.3-1-20251215 (887e7ef) 查看 下载
▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合物信息 Entities & Polymers (8)

▼

6. 折叠分类 (SCOP + CATH) 32 domains

CATH v4.4 (32 domains)

结构域编号 domain_id4lk0A01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1360 — Gyrase A; domain 2
同源超家族 H-superfamily homologous superfamily10 — RNA polymerase, RBP11-like subunit
结构域编号 domain_id4lk0A02
类 Class class2 — Mainly Beta
架构 Architecture architecture170 — Beta Complex
拓扑 Topology topology120 — RNA Polymerase Alpha Subunit; Chain A, domain 2
同源超家族 H-superfamily homologous superfamily12 — DNA-directed RNA polymerase, insert domain
结构域编号 domain_id4lk0B01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1360 — Gyrase A; domain 2
同源超家族 H-superfamily homologous superfamily10 — RNA polymerase, RBP11-like subunit
结构域编号 domain_id4lk0B02
类 Class class2 — Mainly Beta
架构 Architecture architecture170 — Beta Complex
拓扑 Topology topology120 — RNA Polymerase Alpha Subunit; Chain A, domain 2
同源超家族 H-superfamily homologous superfamily12 — DNA-directed RNA polymerase, insert domain
结构域编号 domain_id4lk0C02
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1110 — Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; domain 3
同源超家族 H-superfamily homologous superfamily10 — RNA polymerase Rpb2, domain 2
结构域编号 domain_id4lk0C04
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology150 — Rna Polymerase Beta Subunit; Chain: C, domain 4
同源超家族 H-superfamily homologous superfamily10 — DNA-directed RNA polymerase, beta subunit, external 1 domain
结构域编号 domain_id4lk0C05
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology270 — Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; Domain 6
同源超家族 H-superfamily homologous superfamily10 — DNA-directed RNA polymerase, subunit 2, domain 6
结构域编号 domain_id4lk0C06
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4lk0C07
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily150 — RNA polymerase II, Rpb2 subunit, wall domain
结构域编号 domain_id4lk0C08
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology140 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily1670 —
结构域编号 domain_id4lk0E00
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology940 — Eukaryotic RPB6 RNA polymerase subunit
同源超家族 H-superfamily homologous superfamily10 — RNA polymerase subunit, RPB6/omega
结构域编号 domain_id4lk0F01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology601 — RNA Polymerase Primary Sigma Factor
同源超家族 H-superfamily homologous superfamily10 — RNA Polymerase Primary Sigma Factor
结构域编号 domain_id4lk0F02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
结构域编号 domain_id4lk0F03
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
结构域编号 domain_id4lk0G01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1360 — Gyrase A; domain 2
同源超家族 H-superfamily homologous superfamily10 — RNA polymerase, RBP11-like subunit
结构域编号 domain_id4lk0G02
类 Class class2 — Mainly Beta
架构 Architecture architecture170 — Beta Complex
拓扑 Topology topology120 — RNA Polymerase Alpha Subunit; Chain A, domain 2
同源超家族 H-superfamily homologous superfamily12 — DNA-directed RNA polymerase, insert domain
结构域编号 domain_id4lk0H01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1360 — Gyrase A; domain 2
同源超家族 H-superfamily homologous superfamily10 — RNA polymerase, RBP11-like subunit
结构域编号 domain_id4lk0H02
类 Class class2 — Mainly Beta
架构 Architecture architecture170 — Beta Complex
拓扑 Topology topology120 — RNA Polymerase Alpha Subunit; Chain A, domain 2
同源超家族 H-superfamily homologous superfamily12 — DNA-directed RNA polymerase, insert domain
结构域编号 domain_id4lk0I01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology270 — Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; Domain 6
同源超家族 H-superfamily homologous superfamily10 — DNA-directed RNA polymerase, subunit 2, domain 6
结构域编号 domain_id4lk0I03
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1110 — Dna-directed Rna Polymerase Ii 140kd Polypeptide; Chain: B; domain 3
同源超家族 H-superfamily homologous superfamily10 — RNA polymerase Rpb2, domain 2
结构域编号 domain_id4lk0I05
类 Class class2 — Mainly Beta
架构 Architecture architecture30 — Roll
拓扑 Topology topology150 — Rna Polymerase Beta Subunit; Chain: C, domain 4
同源超家族 H-superfamily homologous superfamily10 — DNA-directed RNA polymerase, beta subunit, external 1 domain
结构域编号 domain_id4lk0I06
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4lk0I07
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily150 — RNA polymerase II, Rpb2 subunit, wall domain
结构域编号 domain_id4lk0I08
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology140 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily1670 —
结构域编号 domain_id4lk0J01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4lk0J02
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily100 — RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain
结构域编号 domain_id4lk0K00
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology940 — Eukaryotic RPB6 RNA polymerase subunit
同源超家族 H-superfamily homologous superfamily10 — RNA polymerase subunit, RPB6/omega
结构域编号 domain_id4lk0L01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology601 — RNA Polymerase Primary Sigma Factor
同源超家族 H-superfamily homologous superfamily10 — RNA Polymerase Primary Sigma Factor
结构域编号 domain_id4lk0L02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
结构域编号 domain_id4lk0L03
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
结构域编号 domain_id4lk0M00
类 Class class3 — Alpha Beta
架构 Architecture architecture10 — Roll
拓扑 Topology topology20 — Ubiquitin-like (UB roll)
同源超家族 H-superfamily homologous superfamily510 — RNA polymerase inhibitor
结构域编号 domain_id4lk0N00
类 Class class3 — Alpha Beta
架构 Architecture architecture10 — Roll
拓扑 Topology topology20 — Ubiquitin-like (UB roll)
同源超家族 H-superfamily homologous superfamily510 — RNA polymerase inhibitor
▶

7. 引用文献 (1)