SAXSdb — 4wls

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1. Protein Identity and Related Structures Protein Identity & Related Structures

HTH-type transcriptional regulator CueR

Escherichia coli DH5[alpha]

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–DNA	Homooligomer	Protein 2 DNA 4	COPA PROMOTER DNA NON-TEMPLATE STRAND × 1 COPA PROMOTER DNA TEMPLATE STRAND × 1 COPA PROMOTER DNA TEMPLATE STRAND (ALTERNATE CONFORMATION) × 1 COPA PROMOTER DNA NON-TEMPLATE STRAND (ALTERNATE CONFORMATION) × 1 water × 4	Review required

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CUER_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–128； UniProt 1–128 Author chain B； PDBConstruct 1–128； UniProt 1–128

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	4wls
Deposition date deposition_date	2014-10-08
Structure title title	Crystal structure of the metal-free (repressor) form of E. Coli CUER, a copper efflux regulator, bound to COPA promoter DNA
Keywords keywords	;PROTEIN-DNA COMPLEX, MERR-FAMILY TRANSCRIPTION REGULATOR, METAL-FREE FORM, REPRESSOR, TRANSCRIPTION, Transcription Regulator-DNA complex ;; Transcription Regulator/DNA
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

4wls__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

4wls__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

4wls__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	25.60 Å
Rg (electron density)	24.49 Å
Total Rg	25.19 Å
Atom count	2854
Residues	268
Excluded volume	47207 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`4wls__assembly_1__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Description description	HTH-type transcriptional regulator CueR
Molecular weight formula_weight	14596.8 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	3973

MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHLNELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMRDQLLALANASPGDDSADSPIIENLSG

Source Source

Source organism organism	Escherichia coli DH5[alpha]
NCBI taxonomy ID ncbi_taxonomy_id	668369
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	COPA PROMOTER DNA NON-TEMPLATE STRAND
Molecular weight formula_weight	7945.1 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	X

TGACCTTCCCCTTGCTGGAAGGTTTA

Entity 3 Entity 3 polymer

Description description	COPA PROMOTER DNA TEMPLATE STRAND
Molecular weight formula_weight	8030.2 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	Y

TAAACCTTCCAGCAAGGGGAAGGTCA

Entity 4 Entity 4 polymer

Description description	COPA PROMOTER DNA TEMPLATE STRAND (ALTERNATE CONFORMATION)
Molecular weight formula_weight	8039.2 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	U

AAACCTTCCAGCAAGGGGAAGGTCAA

Entity 5 Entity 5 polymer

Description description	COPA PROMOTER DNA NON-TEMPLATE STRAND (ALTERNATE CONFORMATION)
Molecular weight formula_weight	7936.1 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	V

TTGACCTTCCCCTTGCTGGAAGGTTT

Entity 6 Entity 6 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	23

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6. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

Domain ID domain_id	4wlsA00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1660 — Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
Homologous superfamily homologous superfamily	10 —

Domain ID domain_id	4wlsB00
Class class	1 — Mainly Alpha
Architecture architecture	10 — Orthogonal Bundle
Topology topology	1660 — Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
Homologous superfamily homologous superfamily	10 —

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Unit-cell length a length_a	52.194 Å
Unit-cell length b length_b	63.653 Å
Unit-cell length c length_c	130.205 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	2.105 Å
Low-resolution limit resolution_low	45.513 Å
R factor r_factor	0.2142
R_work r_work	0.2115
R_free r_free	0.2638

Crystal structure of the metal-free (repressor) form of E. Coli CUER, a copper efflux regulator, bound to COPA promoter DNA

1. Protein Identity and Related Structures Protein Identity & Related Structures

HTH-type transcriptional regulator CueR

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

4wls__assembly_1__model_1

4wls__assembly_1__model_1 | I(q)

4wls__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer

Entity 5 Entity 5 polymer

Polymer Polymer

Entity 6 Entity 6 water

6. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

7. Citations (1)

Crystal structure of the metal-free (repressor) form of E. Coli CUER, a copper efflux regulator, bound to COPA promoter DNA

1. Protein Identity and Related Structures Protein Identity & Related Structures

HTH-type transcriptional regulator CueR

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

4wls__assembly_1__model_1

4wls__assembly_1__model_1 | I(q)

4wls__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer

Entity 5 Entity 5 polymer

Polymer Polymer

Entity 6 Entity 6 water

6. Fold Classification (SCOP + CATH) 2 domains

CATH v4.4 (2 domains)

7. Citations (1)