Macrophage migration inhibitory factor
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein homooligomer Homooligomer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count | Chain A； UniProt 2–115 Chain B； UniProt 2–115 Chain C； UniProt 2–115 | Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 6 GOL GLYCEROL × 4 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；295 K；Tris, ammonium sulfate, isopropanol | Resolution 2.05 Å R-free 0.205 |
| 2 | Protein homooligomer Homooligomer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count | Chain D； UniProt 2–115 Chain E； UniProt 2–115 Chain F； UniProt 2–115 | Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 9 GOL GLYCEROL × 4 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；295 K；Tris, ammonium sulfate, isopropanol | Resolution 2.05 Å R-free 0.205 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 4Z1U | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1CA7 MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH HYDROXPHENYLPYRUVATE Deposited 1999-02-25 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | EN1 (2E)-2-hydroxy-3-(4-hydroxyphenyl)prop-2-enoic acid × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 2.50 Å R-free 0.263 |
| 1CGQ MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH ALANINE INSERTED BETWEEN PRO-1 AND MET-2 Deposited 1999-03-25 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：INS(A-M2) Mutation：INS(A-M2) Mutation：INS(A-M2) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.00 Å R-free 0.275 |
| 1GCZ MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) COMPLEXED WITH INHIBITOR. Deposited 2000-08-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 10 CIT CITRIC ACID × 3 YZ9 7-HYDROXY-2-OXO-CHROMENE-3-CARBOXYLIC ACID ETHYL ESTER × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；ammonium sulfate, sodium citrate, pH 5.0, vapor diffusion/hanging drop, temperature 298.0K
|
Resolution 1.90 Å R-free 0.240 |
| 1GD0 HUMAN MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) Deposited 2000-08-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 7 CIT CITRIC ACID × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；298 K；ammonium sulfate, sodium citrate, pH 5.0, vapor diffusion : hanging drop, temperature 298.0K
|
Resolution 1.50 Å R-free 0.214 |
| 1GIF HUMAN GLYCOSYLATION-INHIBITING FACTOR Deposited 1996-02-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–114（114 aa）
Chain B
1–114（114 aa）
Chain C
1–114（114 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1LJT Crystal Structure of Macrophage Migration Inhibitory Factor complexed with (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole-acetic acid methyl ester (ISO-1) Deposited 2002-04-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–114（114 aa）
Chain B
1–114（114 aa）
Chain C
1–114（114 aa）
|
Not recorded | HDI 3-(4-HYDROXYPHENYL)-4,5-DIHYDRO-5-ISOXAZOLE-ACETIC ACID METHYL ESTER × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；ammonium sulfate, isopropanol, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.262 |
| 1MIF MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) Deposited 1996-01-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1P1G MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH PRO-1 MUTATED TO GLY-1 Deposited 1999-03-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：P1G Mutation：P1G Mutation：P1G | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 2.50 Å R-free 0.270 |
| 2OOH Crystal Structure of MIF bound to a Novel Inhibitor, OXIM-11 Deposited 2007-01-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–114（114 aa）
Chain B
1–114（114 aa）
Chain C
1–114（114 aa）
|
Not recorded | SO4 SULFATE ION × 8 OX3 4-HYDROXYBENZALDEHYDE O-(CYCLOHEXYLCARBONYL)OXIME × 2 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；296 K；50% saturated ammonium sulfate, 4% isopropanol, 0.1 M tris(hydroxymethyl)aminomethane,mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.85 Å R-free 0.218 |
| 2OOW MIF Bound to a Fluorinated OXIM Derivative Deposited 2007-01-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–114（114 aa）
Chain B
1–114（114 aa）
Chain C
1–114（114 aa）
|
Not recorded | SO4 SULFATE ION × 5 OX4 3-FLUORO-4-HYDROXYBENZALDEHYDE O-(CYCLOHEXYLCARBONYL)OXIME × 2 GOL GLYCEROL × 6 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；296 K；50% saturated ammonium sulfate, 4% isopropanol, 0.1
M tris(hydroxymethyl)aminomethan; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.75 Å R-free 0.204 |
| 2OOZ Macrophage Migration Inhibitory Factor (MIF) Complexed with OXIM6 (an OXIM Derivative Not Containing a Ring in its R-group) Deposited 2007-01-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–114（114 aa）
Chain B
1–114（114 aa）
Chain C
1–114（114 aa）
|
Not recorded | SO4 SULFATE ION × 5 OX5 4-HYDROXYBENZALDEHYDE O-(3,3-DIMETHYLBUTANOYL)OXIME × 2 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；296 K；50% saturated ammonium sulfate, 4% isopropanol, 0.1
M tris(hydroxymethyl)aminomethan; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.80 Å R-free 0.225 |
| 3B9S Macrophage Migration Inhibitory Factor (MIF) complexed with Inhibitor, 4-IPP. Deposited 2007-11-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | GOL GLYCEROL × 3 RW1 4-phenylpyrimidine × 3 SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2M ammonium sulfate, 4% isopropanol, 0.1 M tris(hydroxymethyl)aminomethan; mixed with protein:inhibitor complex in a 1:10 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.230 |
| 3CE4 Structure of Macrophage Migration Inhibitory Factor Covalently Inhibited by PMSF Treatment Deposited 2008-02-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 5 PMF PHENYLMETHYLSULFONYL FLUORIDE × 3 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；293 K；50% saturated ammonium sulfate, 4% isopropanol, 0.1M tris(hydroxymethyl)aminomethane; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.55 Å R-free 0.209 |
| 3DJH Macrophage Migration Inhibitory Factor (MIF) at 1.25 A Resolution Deposited 2008-06-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 GOL GLYCEROL × 4 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；50% Saturated ammonium sulfate, 3% Isopropanol, 0.1 M Tris(hydroxymethyl)aminomethane, mixed with protein in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.25 Å R-free 0.186 |
| 3DJI Crystal Structure of Macrophage Migration Inhibitory Factor Bound to an Acetaminophen Dimer Derived from NAPQI Deposited 2008-06-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 9 3E1 N,N'-(6,6'-dihydroxybiphenyl-3,3'-diyl)diacetamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；2 M Ammonium sulfate, 3% Isopropanol, 0.1 M Tris(hydroxymethyl)aminomethane, mixed with protein-inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.95 Å R-free 0.247 |
| 3DJI Crystal Structure of Macrophage Migration Inhibitory Factor Bound to an Acetaminophen Dimer Derived from NAPQI Deposited 2008-06-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；2 M Ammonium sulfate, 3% Isopropanol, 0.1 M Tris(hydroxymethyl)aminomethane, mixed with protein-inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.95 Å R-free 0.247 |
| 3HOF Structure of macrophage migration inhibitory factor (MIF) with caffeic acid at 1.9A resolution Deposited 2009-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Not recorded | DHC CAFFEIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；296 K；30-42.5% MPD_P1K_P3350 mix (MDL Morpheus screen), 0.1M buffer 1 mix (MDL morpheus screen), pH 5.5-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.90 Å R-free 0.200 |
| 3IJG Macrophage Migration Inhibitory Factor (MIF) Bound to the (R)-Stereoisomer of AV1013 Deposited 2009-08-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 10 AVR (2R)-2-amino-1-[2-(1-methylethyl)pyrazolo[1,5-a]pyridin-3-yl]propan-1-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；310 K；2 M ammonium sulfate, 3% isopropanol, 0.5 M NaCl, 0.1 M tris(hydroxymethyl)aminomethane; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K
|
Resolution 1.70 Å R-free 0.206 |
| 3IJJ Ternary Complex of Macrophage Migration Inhibitory Factor (MIF) Bound Both to 4-hydroxyphenylpyruvate and to the Allosteric Inhibitor AV1013 (R-stereoisomer) Deposited 2009-08-04 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | EN1 (2E)-2-hydroxy-3-(4-hydroxyphenyl)prop-2-enoic acid × 3 ENO 3-(4-HYDROXY-PHENYL)PYRUVIC ACID × 3 CL CHLORIDE ION × 8 AVR (2R)-2-amino-1-[2-(1-methylethyl)pyrazolo[1,5-a]pyridin-3-yl]propan-1-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；310 K；2 M ammonium sulfate, 3% isopropanol, 0.5 M NaCl, 0.1 M tris(hydroxymethyl)aminomethane; mixed in a 1:1 ratio with the protein:substrate:inhibitor complex, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K
|
Resolution 1.25 Å R-free 0.185 |
| 3JSF Crystal structure of macrophage migration inhibitory factor (mif) with hydroxyquinoline inhibitor 638 at 1.93a resolution Deposited 2009-09-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | XV1 7-(2-fluorobenzyl)quinolin-8-ol × 3 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M AMMONIUM SULFATE, 2 mM EDTA, 0.1 M HEPES, PH 6.5, VAPOR DIFFUSION/HANGING DROP, TEMPERATURE 298.0K , VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.93 Å R-free 0.218 |
| 3JSG Crystal structure of macrophage migration inhibitory factor (mif) with hydroxyquinoline inhibitor 707 at 1.58a resolution Deposited 2009-09-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 0IN 7-(pyridin-3-ylmethyl)quinolin-8-ol × 3 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M AMMONIUM SULFATE, 2 mM EDTA, 0.1 M HEPES, PH 6.5, VAPOR DIFFUSION/HANGING DROP, TEMPERATURE 298.0K , VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.58 Å R-free 0.201 |
| 3JTU Crystal structure of macrophage migration inhibitory factor (mif) with hydroxyquinoline inhibitor 708 at 1.86a resolution Deposited 2009-09-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | ZIN 7-(pyridin-2-ylmethyl)quinolin-8-ol × 3 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.5 M AMMONIUM SULFATE, 2 mM EDTA, 0.1 M HEPES, PH 6.5, VAPOR DIFFUSION/HANGING DROP, , VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.86 Å R-free 0.192 |
| 3L5P Crystal structure of macrophage migration inhibitory factor (MIF) with imidazopyridazinol inhibitor at 1.80A resolution Deposited 2009-12-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 428 2-(1-methylethyl)imidazo[1,2-b]pyridazin-6-ol × 1 GOL GLYCEROL × 3 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.221 |
| 3L5R Crystal structure of macrophage migration inhibitory factor (MIF) with phenylchromenone inhibitor at 1.94A resolution Deposited 2009-12-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 47X 3-(3,4-dihydroxyphenyl)-7-hydroxy-4H-chromen-4-one × 1 SO4 SULFATE ION × 5 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.94 Å R-free 0.233 |
| 3L5S Crystal structure of macrophage migration inhibitory factor (MIF) with imidazopyrimidinylphenyl inhibitor at 1.86A resolution Deposited 2009-12-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 88X 5-ethyl-2-(phenylcarbonyl)imidazo[1,2-a]pyrimidin-7(1H)-one × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.86 Å R-free 0.201 |
| 3L5T Crystal structure of macrophage migration inhibitory factor (MIF) with thiophenepiperazinylquinolinone inhibitor at 1.86A resolution Deposited 2009-12-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 956 1-methyl-2-oxo-4-[4-(thiophen-2-ylcarbonyl)piperazin-1-yl]-1,2-dihydroquinoline-3-carbonitrile × 1 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.86 Å R-free 0.214 |
| 3L5U Crystal structure of macrophage migration inhibitory factor (MIF) with benzothiazole inhibitor at 1.90A resolution Deposited 2009-12-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | ZEC 6-HYDROXY-1,3-BENZOTHIAZOLE-2-SULFONAMIDE × 3 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.209 |
| 3L5V Crystal structure of macrophage migration inhibitory factor (MIF) with glycerol at 1.70A resolution Deposited 2009-12-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | GOL GLYCEROL × 3 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；0.6 M AMMONIUM SULFATE, 0.1 M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.211 |
| 3SMB Phenethylisothiocyanate Covalently Bound to Macrophage Migration Inhibitory Factor (MIF) Deposited 2011-06-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | LE2 N-(2-phenylethyl)thioformamide × 3 CL CHLORIDE ION × 9 NA SODIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% isopropanol, 0.1
M tris(hydroxymethyl)aminomethane; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.197 |
| 3SMC Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane Deposited 2011-06-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | LE3 N-{4-[(R)-methylsulfinyl]butyl}thioformamide × 3 CL CHLORIDE ION × 7 NA SODIUM ION × 4 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% isopropanol, 0.1
M tris(hydroxymethyl)aminomethane; mixed with protein:inhibitor complex in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.186 |
| 3U18 Chicago Sky Blue 6B, A Novel Inhibitor for Macrophage Migration Inhibitory Factor Deposited 2011-09-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | Y0X 6,6'-[(3,3'-dimethoxybiphenyl-4,4'-diyl)di(E)diazene-2,1-diyl]bis(4-amino-5-hydroxynaphthalene-1,3-disulfonic acid) × 1 IPA ISOPROPYL ALCOHOL × 4 GOL GLYCEROL × 9 EDO 1,2-ETHANEDIOL × 3 NA SODIUM ION × 4 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8；310 K；100 mM p425 dissolved in 2.0 M ammonium sulfate, 4% 2-propanol, 0.1 M Tris, VAPOR DIFFUSION, temperature 310K, pH 8
|
Resolution 1.90 Å R-free 0.177 |
| 3WNR Multiple binding modes of benzyl isothiocyanate inhibitor complexed with Macrophage Migration Inhibitory Factor Deposited 2013-12-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 9BE N-benzylthioformamide × 3 SO4 SULFATE ION × 5 GOL GLYCEROL × 5 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.9M ammonium sulfate, 100mM Tris pH 8.0, 200mM NaCl, 4%(v/v) 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.01 Å R-free 0.200 |
| 3WNS Allyl isothiocyanate inhibitor complexed with Macrophage Migration Inhibitory Factor Deposited 2013-12-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 9AI N-prop-2-en-1-ylthioformamide × 3 SO4 SULFATE ION × 6 IPA ISOPROPYL ALCOHOL × 1 CL CHLORIDE ION × 1 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.9M ammonium sulfate, 100mM Tris pH 8.0, 200mM NaCl, 4%(v/v) 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.66 Å R-free 0.258 |
| 3WNT Multiple binding modes of benzyl isothiocyanate inhibitor complexed with Macrophage Migration Inhibitory Factor Deposited 2013-12-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 9BE N-benzylthioformamide × 3 SO4 SULFATE ION × 5 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.9M ammonium sulfate, 100mM Tris pH 8.0, 200mM NaCl, 4%(v/v) 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.07 Å R-free 0.263 |
| 4ETG Crystal Structure of MIF L46G mutant Deposited 2012-04-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：L46G Mutation：L46G Mutation：L46G | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；291 K；1.8M Ammonium sulfate in 0.1M Tris and 3% isopropanol, pH 7.5, VAPOR DIFFUSION, temperature 291K
|
Resolution 1.61 Å R-free 0.249 |
| 4EUI Crystal Structure of MIF L46F mutant Deposited 2012-04-25 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：L46F Mutation：L46F Mutation：L46F | SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；291 K；1.8M Ammonium sulfate in 0.1M Tris and 3% isopropanol, pH 7.5, VAPOR DIFFUSION, temperature 291K
|
Resolution 1.70 Å R-free 0.259 |
| 4EVG Crystal Structure of MIF L46A mutant Deposited 2012-04-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：L46A Mutation：L46A Mutation：L46A | SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；291 K；1.8M Ammonium sulfate in 0.1M Tris (pH 7.5) and 3% isopropanol, VAPOR DIFFUSION, temperature 291K
|
Resolution 1.70 Å R-free 0.214 |
| 4F2K Macrophage Migration Inhibitory Factor covalently complexed with phenethylisothiocyanate Deposited 2012-05-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | LE2 N-(2-phenylethyl)thioformamide × 3 SO4 SULFATE ION × 4 IPA ISOPROPYL ALCOHOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；1.9M (NH4)2SO4, 100mM Tris pH 8.0, 200mM NaCl, 4% v/v isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.53 Å R-free 0.228 |
| 4GRN crystal structure of PAAM mutant of human MIF Deposited 2012-08-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 12 SO4 SULFATE ION × 4 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2M NH42SO4, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.25 Å R-free 0.141 |
| 4GRO Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0 ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.230 |
| 4GRO Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
Chain D
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0 ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.230 |
| 4GRO Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
Chain H
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | CL CHLORIDE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0 ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.230 |
| 4GRO Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain G
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions | CL CHLORIDE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0 ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.230 |
| 4GRP Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | SO4 SULFATE ION × 5 CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.6；293 K；1.6M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.27 Å R-free 0.169 |
| 4GRQ Characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | CL CHLORIDE ION × 6 SO4 SULFATE ION × 1 AVL 2-methyl-1-[2-(propan-2-yl)pyrazolo[1,5-a]pyridin-3-yl]propan-1-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；310 K；1.6M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K
|
Resolution 1.65 Å R-free 0.161 |
| 4GRR characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
3–115（113 aa）
Chain B
3–115（113 aa）
Chain C
3–115（113 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | SO4 SULFATE ION × 5 CL CHLORIDE ION × 11 AVR (2R)-2-amino-1-[2-(1-methylethyl)pyrazolo[1,5-a]pyridin-3-yl]propan-1-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；310 K；1.6M ammmonium sualfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K
|
Resolution 1.47 Å R-free 0.172 |
| 4GRU crystallographic and biological characterization of N- and C- terminus mutants of human MIF Deposited 2012-08-26 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions Mutation：Pro1-Ala2 are insertions | CL CHLORIDE ION × 12 DIO 1,4-DIETHYLENE DIOXIDE × 3 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.0M ammonium sulfate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.92 Å R-free 0.230 |
| 4GUM Cystal structure of locked-trimer of human MIF Deposited 2012-08-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：N110C Mutation：N110C Mutation：N110C | CL CHLORIDE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；0.2M LiSO4, 3% DMSO, pH8.0, 33% PEG4000 , VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.33 Å R-free 0.272 |
| 4GUM Cystal structure of locked-trimer of human MIF Deposited 2012-08-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Mutation：N110C Mutation：N110C Mutation：N110C | CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；0.2M LiSO4, 3% DMSO, pH8.0, 33% PEG4000 , VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.33 Å R-free 0.272 |
| 4GUM Cystal structure of locked-trimer of human MIF Deposited 2012-08-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain G
2–115（114 aa）
Chain H
2–115（114 aa）
Chain I
2–115（114 aa）
|
Mutation：N110C Mutation：N110C Mutation：N110C | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；0.2M LiSO4, 3% DMSO, pH8.0, 33% PEG4000 , VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.33 Å R-free 0.272 |
| 4K9G 1.55 A Crystal Structure of Macrophage Migration Inhibitory Factor bound to ISO-66 and a related compound Deposited 2013-04-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 1Q2 (4R,6Z)-6-(3-fluoro-4-hydroxyphenyl)-4-hydroxy-6-iminohexan-2-one × 3 1Q1 1-[(5S)-3-(3-fluoro-4-hydroxyphenyl)-4,5-dihydro-1,2-oxazol-5-yl]propan-2-one × 1 CL CHLORIDE ION × 6 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；293 K；1.1 mM (14 mg/ml) MIF , 10 mM inhibitor in 10% DMSO, 18 mM NaCl, 18 mM tris(hydroxymethyl)aminomethane mixed 1:1 with reservoir containing 2 M ammonium sulfate, 0.1 M tris(hydroxymethyl)aminomethane, 3% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.55 Å R-free 0.220 |
| 4OSF 4-(2-isothiocyanatoethyl)phenol inhibitor complexed with Macrophage Migration Inhibitory Factor Deposited 2014-02-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 4MT N-[2-(4-hydroxyphenyl)ethyl]thioformamide × 3 SO4 SULFATE ION × 12 CL CHLORIDE ION × 2 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.9M ammonium sulfate, 100mM Tris pH 8.0, 200mM NaCl, 4%(v/v) 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.62 Å R-free 0.223 |
| 4OYQ (6-isothiocyanatohexyl)benzene inhibitor complexed with Macrophage Migration Inhibitory Factor Deposited 2014-02-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 1X2 6-isothiocyanatohexylbenzene × 3 SO4 SULFATE ION × 4 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；1.9 M ammonium sulfate, 100 mM Tris pH 8.0, 200 mM NaCl, 4%(v/v) 2-propanol
|
Resolution 1.70 Å R-free 0.195 |
| 4P01 Crystal Structure Analysis of Macrophage Migration Inhibitory Factor in complex with N-[(4-cyanophenyl)methyl]methanethioamide Deposited 2014-02-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 1ZW N-[(4-cyanophenyl)methyl]methanethioamide × 2 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 1 CL CHLORIDE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol, 20 mM Tris.HCl, pH 7.5
|
Resolution 2.07 Å R-free 0.228 |
| 4P0H Crystal Structure Analysis of Macrophage Migration Inhibitory Factor in complex with Dimethylformamide Deposited 2014-02-21 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | DMF DIMETHYLFORMAMIDE × 3 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol,20 mM Tris.HCl pH 7.5
|
Resolution 1.93 Å R-free 0.217 |
| 4PKK Crystal structure of Macrophage Migration inhibitory factor in complex with furan-2-ylmethyl)imino methanethiol Deposited 2014-05-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 31E (Z)-[(furan-2-ylmethyl)imino]methanethiol × 3 GOL GLYCEROL × 4 SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol, 20 mM Tris.HCl, pH 7.5
|
Resolution 1.78 Å R-free 0.181 |
| 4PKZ Crystal structure of P1M mutant of Macrophage Migration Inhibitory Factor Deposited 2014-05-15 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：P1M Mutation：P1M Mutation：P1M | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol, 20 mM Tris.HCl, pH 7.5
|
Resolution 1.90 Å R-free 0.251 |
| 4PLU Crystal structure of Macrophage Migration Inhibitory Factor in complex with benzaldehyde Deposited 2014-05-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | HBX benzaldehyde × 3 SO4 SULFATE ION × 2 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol,20 mM Tris.HCl pH 7.5
|
Resolution 1.63 Å R-free 0.214 |
| 4TRF Crystal structure of Macrophage Migration Inhibitory Factor in complex with N-(pyridin-3-ylmethyl)thioformamide Deposited 2014-06-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 35J N-(pyridin-3-ylmethyl)thioformamide × 3 SO4 SULFATE ION × 3 GOL GLYCEROL × 2 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol,20 mM Tris.HCl pH 7.5
|
Resolution 1.63 Å R-free 0.181 |
| 4TRU Crystal structure of Q24A Q25A mutant of human macrophage migration inhibitory factor Deposited 2014-06-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Q24A, Q25A Mutation：Q24A, Q25A Mutation：Q24A, Q25A | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 3 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol,20 mM Tris.HCl pH 7.5
|
Resolution 1.81 Å R-free 0.191 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 9 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 9 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain G
2–115（114 aa）
Chain H
2–115（114 aa）
Chain I
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 9 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain J
2–115（114 aa）
Chain K
2–115（114 aa）
Chain L
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 9 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain M
2–115（114 aa）
Chain N
2–115（114 aa）
Chain O
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 8 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WR8 Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180) Deposited 2014-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain P
2–115（114 aa）
Chain Q
2–115（114 aa）
Chain R
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 9 3TX 4-[4-(quinolin-2-yl)-1H-1,2,3-triazol-1-yl]phenol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 2.60 Å R-free 0.280 |
| 4WRB Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-190) Deposited 2014-10-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 3TW 4-{4-[6-(2-methoxyethoxy)quinolin-2-yl]-1H-1,2,3-triazol-1-yl}phenol × 1 SO4 SULFATE ION × 6 IPA ISOPROPYL ALCOHOL × 4 GOL GLYCEROL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.2M ammonium sulfate, 0.1M Tris-HCl pH 7, 3% isopropanol
|
Resolution 1.81 Å R-free 0.205 |
| 4XX7 Crystal structure of M2A mutant of human macrophage migration inhibitory factor Deposited 2015-01-29 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Fragment：UNP residues
Chain B
2–115（114 aa）
Fragment：UNP residues
Chain C
2–115（114 aa）
Fragment：UNP residues
|
Mutation：M2A Mutation：M2A Mutation：M2A | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；2 M ammonium Sulphate, 3% 2-propanol, 20 mM Tris.HCl, pH 7.5
|
Resolution 1.77 Å R-free 0.187 |
| 4XX8 Crystal structure of Pro1 deletion mutant of human macrophage migration inhibitory factor Deposited 2015-01-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
3–115（113 aa）
Chain B
3–115（113 aa）
Chain C
3–115（113 aa）
|
Mutation：Pro1 deletion Mutation：Pro1 deletion Mutation：Pro1 deletion | SO4 SULFATE ION × 5 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；291 K；2 M ammonium Sulphate, 3% 2-propanol, 20 mM Tris.HCl, pH 7.5
|
Resolution 1.77 Å R-free 0.188 |
| 4Z15 MIF in complex with 3-(2-furylmethyl)-2-thioxo-1,3-thiazolan-4-one Deposited 2015-03-26 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | IPA ISOPROPYL ALCOHOL × 1 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；Tris, ammonium sulfate, isopropanol
|
Resolution 1.60 Å R-free 0.210 |
| 4Z1T MIF in complex with 4-[(4-oxo-2-thioxo-1,3-thiazolan-3-yl)methyl]benzonitrile Deposited 2015-03-27 | Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | IPA ISOPROPYL ALCOHOL × 2 SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；Tris, ammonium sulfate, isopropanol
|
Resolution 1.50 Å R-free 0.225 |
| 5B4O Crystal structure of Macrophage Migration Inhibitory Factor in complex with BTZO-14 Deposited 2016-04-07 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Fragment：UNP residues 2-115
Chain B
2–115（114 aa）
Fragment：UNP residues 2-115
Chain C
2–115（114 aa）
Fragment：UNP residues 2-115
|
Not recorded | 6DQ 2-pyridin-3-yl-1,3-benzothiazin-4-one × 4 EDO 1,2-ETHANEDIOL × 1 SO4 SULFATE ION × 9 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；2.4M ammonium sulfate, 0.1M bicine pH 9.0
, 2.4 mM BTZO-14
|
Resolution 1.37 Å R-free 0.198 |
| 5BS9 Crystal structure of N109A mutant of human macrophage migration inhibitory factor Deposited 2015-06-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：N109A Mutation：N109A Mutation：N109A | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 6 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.98 Å R-free 0.185 |
| 5BSC Crystal structure of K66A mutant of human macrophage migration inhibitory factor Deposited 2015-06-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：K66A Mutation：K66A Mutation：K66A | IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.89 Å R-free 0.218 |
| 5BSI Crystal structure of Y36A mutant of human macrophage migration inhibitory factor Deposited 2015-06-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Y36A Mutation：Y36A Mutation：Y36A | SO4 SULFATE ION × 3 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.221 |
| 5BSI Crystal structure of Y36A mutant of human macrophage migration inhibitory factor Deposited 2015-06-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Mutation：Y36A Mutation：Y36A Mutation：Y36A | SO4 SULFATE ION × 3 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.221 |
| 5BSI Crystal structure of Y36A mutant of human macrophage migration inhibitory factor Deposited 2015-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain G
2–115（114 aa）
|
Mutation：Y36A | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.221 |
| 5BSI Crystal structure of Y36A mutant of human macrophage migration inhibitory factor Deposited 2015-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain J
2–115（114 aa）
|
Mutation：Y36A | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.221 |
| 5BSJ Crystal structure of S63A mutant of human macrophage migration inhibitory factor Deposited 2015-06-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：S63A Mutation：S63A Mutation：S63A | SO4 SULFATE ION × 4 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.00 Å R-free 0.210 |
| 5EIZ Crystal structure of Y99A mutant of human macrophage migration inhibitory factor Deposited 2015-10-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Y99A Mutation：Y99A Mutation：Y99A | GOL GLYCEROL × 3 SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.96 Å R-free 0.181 |
| 5HVS Crystal Structure of Macrophage Migration Inhibitory Factor (MIF) with a Biaryltriazole Inhibitor (3i-305) Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 65V 3-({2-[1-(3-fluoro-4-hydroxyphenyl)-1H-1,2,3-triazol-4-yl]quinolin-5-yl}oxy)benzoic acid × 3 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；2.0M ammonium sulfate, 0.1M Tris-HCl pH 8, 3% isopropanol
|
Resolution 1.75 Å R-free 0.206 |
| 5HVT Crystal Structure of Macrophage Migration Inhibitory Factor (MIF) with a Potent Inhibitor (NVS-2) Deposited 2016-01-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 NVS 7-hydroxy-3-(4-methoxyphenyl)-3,4-dihydro-2H-1,3-benzoxazin-2-one × 3 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.0 M Ammonium Sulfate, 3% isopropanol and 0.1 M Tris pH 7.0
|
Resolution 1.75 Å R-free 0.203 |
| 5HVV Crystal structure of Pro1 deletion and M2A double mutant of Macrophage Migration Inhibitory Factor Deposited 2016-01-28 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
3–115（113 aa）
Fragment：UNP residues 3-113
Chain B
3–115（113 aa）
Fragment：UNP residues 3-113
Chain C
3–115（113 aa）
Fragment：UNP residues 3-113
|
Mutation：M2A Mutation：M2A Mutation：M2A | SO4 SULFATE ION × 1 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.90 Å R-free 0.191 |
| 5J7P Macrophage Migration Inhibitory Factor bound to Covalent Inhibitor RDR03785 Deposited 2016-04-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 6H1 6-{[4-(trifluoromethyl)phenyl]methyl}-2H-1,3-benzodioxol-5-ol × 3 SO4 SULFATE ION × 7 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.2 M Ammonium Sulfate, 3% isopropanol and 0.1 M Tris pH 7.0
|
Resolution 1.85 Å R-free 0.201 |
| 5J7Q Macrophage Migration Inhibitory Factor bound to Inhibitor K664 Derivative Deposited 2016-04-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 6H2 4-(imidazo[1,2-a]pyridin-2-yl)benzene-1,2-diol × 1 SO4 SULFATE ION × 7 GOL GLYCEROL × 2 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；2.4 M Ammonium Sulfate, 3% isopropanol and 0.1 M Tris pH 7.0
|
Resolution 2.05 Å R-free 0.233 |
| 5UMJ Crystal structure of H62A mutant of human macrophage migration inhibitory factor Deposited 2017-01-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：H62A Mutation：H62A Mutation：H62A | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.61 Å R-free 0.179 |
| 5UMK Crystal structure of H62Y mutant of human macrophage migration inhibitory factor Deposited 2017-01-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：H62Y Mutation：H62Y Mutation：H62Y | SO4 SULFATE ION × 2 IPA ISOPROPYL ALCOHOL × 4 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.73 Å R-free 0.175 |
| 5UZY Crystal structure of N97A mutant of human macrophage migration inhibitory factor Deposited 2017-02-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：N97A Mutation：N97A Mutation：N97A | SO4 SULFATE ION × 4 IPA ISOPROPYL ALCOHOL × 3 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.71 Å R-free 0.178 |
| 5V70 Crystal structure of N102A mutant of human macrophage migration inhibitory factor Deposited 2017-03-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：N102A Mutation：N102A Mutation：N102A | SO4 SULFATE ION × 5 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.94 Å R-free 0.198 |
| 5V73 Crystal structure of N110A mutant of human macrophage migration inhibitory factor Deposited 2017-03-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：N110A Mutation：N110A Mutation：N110A | SO4 SULFATE ION × 4 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.0 - 8.0
|
Resolution 1.68 Å R-free 0.175 |
| 5XEJ Crystal Structure of Macrophage Migration Inhibitory Factor bound to MTX Deposited 2017-04-05 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Fragment：UNP residues 2-115
Chain B
2–115（114 aa）
Fragment：UNP residues 2-115
Chain C
2–115（114 aa）
Fragment：UNP residues 2-115
|
Not recorded | SO4 SULFATE ION × 4 6UV (2~{R})-2-[[4-[[2,4-bis(azanyl)pteridin-6-yl]methyl-methyl-amino]phenyl]carbonylamino]pentanedioic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1M HEPES(pH7.5), 65% Sat. AS, 2% PEG400
|
Resolution 2.50 Å R-free 0.227 |
| 6B1C Macrophage Migration Inhibitory Factor in complex with a Naphthyridinone Inhibitor (4a) Deposited 2017-09-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 4 C9J 2-[1-(3-fluoro-4-hydroxyphenyl)-1H-1,2,3-triazol-4-yl]-7-methyl-1,7-naphthyridin-8(7H)-one × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；2.6 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 7, 3% ISOPROPANOL, 2% DMSO
|
Resolution 2.16 Å R-free 0.254 |
| 6B1K Macrophage Migration Inhibitory Factor in Complex with a Naphthyridinone Inhibitor (3a) Deposited 2017-09-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | C9G 2-[1-(4-hydroxyphenyl)-1H-1,2,3-triazol-4-yl]-7-methyl-1,7-naphthyridin-8(7H)-one × 3 SO4 SULFATE ION × 4 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293.15 K；2.1 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 7.5, 3% ISOPROPANOL, 1.4% DMSO
|
Resolution 1.17 Å R-free 0.154 |
| 6B2C Macrophage Migration Inhibitory Factor in Complex with a Naphthyridinone Inhibitor (4b) Deposited 2017-09-19 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 1 C9Y {2-[1-(3-fluoro-4-hydroxyphenyl)-1H-1,2,3-triazol-4-yl]-8-oxo-1,7-naphthyridin-7(8H)-yl}acetic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293.15 K；2.1 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 7.5, 3% ISOPROPANOL, 2% DMSO
|
Resolution 2.00 Å R-free 0.222 |
| 6BG6 Crystal structure of S111A mutant of human macrophage migration inhibitory factor Deposited 2017-10-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：S111A Mutation：S111A Mutation：S111A | SO4 SULFATE ION × 3 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M Ammonium Sulphate, 3% 2-propanol, 100 mM Tris.HCl, pH 7.5
|
Resolution 1.52 Å R-free 0.174 |
| 6BG7 Crystal structure of G107A mutant of human macrophage migration inhibitory factor Deposited 2017-10-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：G107A Mutation：G107A Mutation：G107A | SO4 SULFATE ION × 4 IPA ISOPROPYL ALCOHOL × 3 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 2.54 Å R-free 0.225 |
| 6CB5 Macrophage Migration Inhibitory Factor in complex with a Pyrazole Inhibitor (8g) Deposited 2018-02-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | EV7 2-[(naphthalen-2-yl)oxy]-5-(1H-pyrazol-4-yl)benzoic acid × 3 SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；2.0 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 7.0, 3% ISOPROPANOL
|
Resolution 1.78 Å R-free 0.212 |
| 6CBF Macrophage Migration Inhibitory Factor in Complex with a Pyrazole Inhibitor (6a) Deposited 2018-02-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | EWD 2-phenoxy-5-(1H-pyrazol-4-yl)benzoic acid × 2 SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；2.4M ammonium sulfate, 3% isopropanol, 0.1M Tris pH 6.5
|
Resolution 2.30 Å R-free 0.282 |
| 6CBG Macrophage Migration Inhibitory Factor in Complex with a Pyrazole Inhibitor (5) Deposited 2018-02-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 GOL GLYCEROL × 9 EWG 3-(1H-pyrazol-4-yl)benzoic acid × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293.15 K；2.4M ammonium sulfate, 3% isopropanol, 0.1M Tris pH 7
|
Resolution 2.00 Å R-free 0.205 |
| 6CBH Macrophage Migration Inhibitory Factor in Complex with a Pyrazole Inhibitor (8m) Deposited 2018-02-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 2 EWJ 5-(3-fluoro-1H-pyrazol-4-yl)-2-[(naphthalen-2-yl)oxy]benzoic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.4M ammonium sulfate, 3% isopropanol, 0.1M Tris pH 7
|
Resolution 2.00 Å R-free 0.214 |
| 6FVE Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound FITC Deposited 2018-03-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | 6B9 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-5-[(E)-(sulfanylmethylidene)amino]benzoic acid × 3 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Ammonium Sulfate, 0.1M Hepes pH 7.0
|
Resolution 1.41 Å R-free 0.189 |
| 6FVH Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound PITC Deposited 2018-03-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 6 0FI N-phenylthioformamide × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；Ammonium Sulfate, Hepes
|
Resolution 1.40 Å R-free 0.166 |
| 6OY8 Crystal structure of Y99G mutant of human macrophage migration inhibitory factor Deposited 2019-05-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Y99G Mutation：Y99G Mutation：Y99G | SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 2 GOL GLYCEROL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.53 Å R-free 0.175 |
| 6OYB Crystal structure of H62G mutant of human macrophage migration inhibitory factor Deposited 2019-05-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：H62G Mutation：H62G Mutation：H62G | SO4 SULFATE ION × 4 GOL GLYCEROL × 5 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.53 Å R-free 0.161 |
| 6OYE Crystal structure of Y99F mutant of human macrophage migration inhibitory factor Deposited 2019-05-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Y99F Mutation：Y99F Mutation：Y99F | GOL GLYCEROL × 4 SO4 SULFATE ION × 3 IPA ISOPROPYL ALCOHOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.53 Å R-free 0.168 |
| 6OYG Crystal structure of H62F mutant of human macrophage migration inhibitory factor Deposited 2019-05-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：H62F Mutation：H62F Mutation：H62F | SO4 SULFATE ION × 3 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.55 Å R-free 0.154 |
| 6PEG MIF with a allosteric inhibitor Deposited 2019-06-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain D
2–115（114 aa）
Chain E
2–115（114 aa）
Chain F
2–115（114 aa）
|
Not recorded | ACT ACETATE ION × 4 GOL GLYCEROL × 2 SO4 SULFATE ION × 3 4FQ 4-amino-5-hydroxy-6-[(E)-(3-{[3-(2-methylpropanoyl)pyrazolo[1,5-a]pyridin-2-yl]methyl}phenyl)diazenyl]naphthalene-1,3-disulfonic acid × 1 PO4 PHOSPHATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；100microMolar 4048, 2M Ammonium sulfate, 4% isopropanol, 50 mM Tris pH 8.0, 12.5% Glycerol, 50mM sodium acetate
Protein 100mg/ml MIF in 50mM citrate pH 5, 50microMolar 4048
|
Resolution 2.00 Å R-free 0.188 |
| 7E45 Crystal structure of compound 7 bound to MIF Deposited 2021-02-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 13 HWO (2R)-7-[(2R,3R,4R,5S,6S)-6-(hydroxymethyl)-3-[(2R,3R,4R,5R,6S)-6-methyl-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,5-bis(oxidanyl)oxan-2-yl]oxy-2-(4-methoxy-3-oxidanyl-phenyl)-5-oxidanyl-2,3-dihydrochromen-4-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.0 M ammonium sulfate, 0.1 M Tris pH 7.0, 3% isopropanol
|
Resolution 1.43 Å R-free 0.186 |
| 7E47 Crystal structure of compound 6 bound to MIF Deposited 2021-02-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 7 HWR (2S,3R,4R,5R,6R)-2-[(2S,3R,4S,5R,6S)-6-(hydroxymethyl)-2-[[(2R,4R)-2-(4-hydroxyphenyl)-4,5-bis(oxidanyl)-3,4-dihydro-2H-chromen-7-yl]oxy]-4,5-bis(oxidanyl)oxan-3-yl]oxy-6-methyl-oxane-3,4,5-triol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.0M ammonium sulfate, 3% isopropanol
|
Resolution 1.38 Å R-free 0.179 |
| 7E49 Crystal structure of MIF bound to compound10 Deposited 2021-02-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 8 MYC 3,5,7-TRIHYDROXY-2-(3,4,5-TRIHYDROXYPHENYL)-4H-CHROMEN-4-ONE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；2.0M ammonium sulfate, 3% isopropanol
|
Resolution 1.57 Å R-free 0.235 |
| 7E4A Crystal structure of MIF bound to compound 13 Deposited 2021-02-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 11 HWL Flavoxate × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；310 K；2.0M ammonium sulfate
|
Resolution 1.48 Å R-free 0.207 |
| 7E4B Crystal structure of MIF bound to compound 5 Deposited 2021-02-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 4 MRI 2-[2,4-bis(oxidanyl)phenyl]-3,5,7-tris(oxidanyl)chromen-4-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；310 K；2.0M ammonium, 3% isopropanol
|
Resolution 1.77 Å R-free 0.232 |
| 7E4C Crystal structure of MIF bound to compound11 Deposited 2021-02-11 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 13 SO4 SULFATE ION × 4 GBJ 4-[(3R)-8,8-dimethyl-3,4-dihydro-2H,8H-pyrano[2,3-f]chromen-3-yl]benzene-1,3-diol × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；310 K；2.0M ammonium sulfate, 3% isopropanol
|
Resolution 1.64 Å R-free 0.191 |
| 7EDQ MIF complex to compound7 Deposited 2021-03-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | HFC 6,7-bis(oxidanyl)chromen-2-one × 1 CL CHLORIDE ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；290 K；2.0M Ammonium sulfate
|
Resolution 1.27 Å R-free 0.190 |
| 7EE8 The crystal structure of MIF bound to compound D5 Deposited 2021-03-17 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | CL CHLORIDE ION × 9 7Y8 3-[(2R)-2-azanyl-1-oxidanyl-propyl]phenol × 1 J23 3-[(2S)-2-azanyl-1-oxidanyl-propyl]phenol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；310 K；2.0M Ammonium sulfate, pH7.50
|
Resolution 1.22 Å R-free 0.242 |
| 7KQX MIF Y99C homotrimeric mutant Deposited 2020-11-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：Y99C Mutation：Y99C Mutation：Y99C | SO4 SULFATE ION × 3 GOL GLYCEROL × 15 IPA ISOPROPYL ALCOHOL × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.60 Å R-free 0.180 |
| 7XTX High resolution crystal structure of human macrophage migration inhibitory factor in complex with methotrexate Deposited 2022-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 4 EDO 1,2-ETHANEDIOL × 11 IPA ISOPROPYL ALCOHOL × 3 MT1 N-(4-{[(2,4-DIAMINOPTERIDIN-1-IUM-6-YL)METHYL](METHYL)AMINO}BENZOYL)-L-GLUTAMIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；283 K；2.0 M Ammonium sulfate
0.1 M Tris-HCl (pH 8.0)
4 % isopropanol
|
Resolution 1.28 Å R-free 0.188 |
| 7XVX Neutron crystal structure of human macrophage migration inhibitory factor Deposited 2022-05-25 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | No recorded non-water small molecule |
Experimental method not declared
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；283 K；0.49 M Monosodium phosphate, 0.91 M Dipotassium phosphate
|
Resolution not provided |
| 8CA0 Macrophage inhibitory factor (MIF) in complex with small molecule PAV174 Deposited 2023-01-24 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | VIU 1-ethyl-9-methyl-3,7-dipyrrolidin-1-yl-phenothiazine × 1 |
SOLUTION NMR
NMR measurement conditions
pH 7.2；298 K；Ionic strength (raw mmCIF value) 20；Pressure 1
NMR sample composition
1.0 mM [U-13C; U-15N] MIF, 1.0 mM PAV, 100% D2O | 100% D2O
|
Resolution not provided |
| 8IMR Structure of ligand-free human macrophage migration inhibitory factor Deposited 2023-03-07 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | SO4 SULFATE ION × 2 EDO 1,2-ETHANEDIOL × 6 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；283 K；2.0 M Ammonium sulfate
0.1 M Tris-HCl (pH 8.0)
4 % Isopropanol
|
Resolution 1.30 Å R-free 0.217 |
| 8SON Crystal structure of macrophage migration inhibitory factor in complex with N-[3-(Trifluoromethyl)phenyl]-3-(2-chloroanilino)-2-cyano-3-thioxopropanamide Deposited 2023-04-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | GOL GLYCEROL × 3 SO4 SULFATE ION × 3 30Y (2R)-3-[(2-chlorophenyl)amino]-2-cyano-3-thioxo-N-[3-(trifluoromethyl)phenyl]propanamide × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；20 mM Tris.HCl, pH 7.5, 2 M ammonium sulfate, and 3% 2-propanol
|
Resolution 1.63 Å R-free 0.199 |
| 8SPN Crystal structure of macrophage migration inhibitory factor in complex with T614 Deposited 2023-05-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Mutation：No Mutation：No Mutation：No | SO4 SULFATE ION × 2 IPA ISOPROPYL ALCOHOL × 1 GOL GLYCEROL × 1 7TN Iguratimod × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2 M ammonium sulfate, 3 % 2-propanol, 0.1 M Tris-HCl, pH 7.5
|
Resolution 1.73 Å R-free 0.202 |
| 8TT8 Joint Xray/Neutron structure of Macrophage Migration Inhibitory Factor (MIF) Bound to 4-hydroxyphenylpyruvate at room temperature Deposited 2023-08-13 | Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | ENO 3-(4-HYDROXY-PHENYL)PYRUVIC ACID × 2 IPA ISOPROPYL ALCOHOL × 1 EN1 (2E)-2-hydroxy-3-(4-hydroxyphenyl)prop-2-enoic acid × 1 |
Experimental method not declared
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；295 K；Protein: 1 mM MIF, 10 mM deuterated HPP, 20 mM NaCl in 20 mM Tris (pH 7.4) mixed 1:1 with reservoir containing 2M (NH4)2SO4, 1% isopropanol in 100 mM Tris (pH 7.4).
|
Resolution not provided |
| 8TT9 X-ray structure of Macrophage Migration Inhibitory Factor (MIF) Covalently Bound to 4-hydroxyphenylpyruvate (HPP) Deposited 2023-08-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | ENO 3-(4-HYDROXY-PHENYL)PYRUVIC ACID × 3 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；2M AMMONIUM SULFATE, 4% ISOPROPANOL, 0.1 M TRIS(HYDROXYMETHYL)AMINOMETHAN; MIXED WITH PROTEIN:INHIBITOR COMPLEX IN A 1:10 RATIO
|
Resolution 1.68 Å R-free 0.199 |
| 9BNQ N-(4-(isothiocyanatomethyl)phenyl)methanesulfonamide complexed with Macrophage Migration Inhibitory Factor Deposited 2024-05-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | A1AQU N-{[4-(methanesulfonamido)phenyl]methyl}methanethioamide × 3 SO4 SULFATE ION × 5 IPA ISOPROPYL ALCOHOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289.15 K；0.2 uL 11 mg/mL MIF (100 mM Tris-HCl pH 8.0, 200 mM NaCl) were mixed with 0.2 uL of reservoir solution. Sitting drop reservoir contained 33 uL of 2.1 M Ammonium sulfate, 0.1 M Tris pH 7.5, 3% Isopropanol and <5mM N-(4-(isothiocyanatomethyl)phenyl)methanesulfonamide (10% DMSO final). Crystal was frozen in a solution of ~25% Glycerol, 75% reservoir
|
Resolution 1.09 Å R-free 0.154 |
| 9BNR 4-(2-isothiocyanatoethyl)benzenesulfonamide complexed with Macrophage Migration Inhibitory Factor Deposited 2024-05-02 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
2–115（114 aa）
Chain B
2–115（114 aa）
Chain C
2–115（114 aa）
|
Not recorded | A1AQT N-[2-(4-sulfamoylphenyl)ethyl]methanethioamide × 3 SO4 SULFATE ION × 9 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；289.15 K；0.2 uL 11 mg/mL MIF (100 mM Tris-HCl pH 8.0, 200 mM NaCl) were mixed with 0.2 uL of reservoir solution. Sitting drop reservoir contained 33 uL of 2.0 M Ammonium sulfate, 0.1 M HEPES pH 7.0, 3% Isopropanol and <5mM 4-(2-isothiocyanatoethyl)benzenesulfonamide (10% DMSO final). Crystal was frozen in a solution of ~25% Glycerol, 75% reservoir
|
Resolution 1.53 Å R-free 0.178 |
| 9JIT Macrophage migration inhibitory factor S61H/Y100H mutant (MIF(S61H/Y100H)) Deposited 2024-09-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Mutation：S61H/Y100H Mutation：S61H/Y100H Mutation：S61H/Y100H | SO4 SULFATE ION × 4 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2.0M ammonium sulfate, 20mM Tris-HCl buffer (pH 7.5), 3% isopropanol
|
Resolution 1.08 Å R-free 0.155 |
| 9JIV Macrophage migration inhibitory factor Y100H mutant (MIF(Y100H)) Deposited 2024-09-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Mutation：Y100H Mutation：Y100H Mutation：Y100H | SO4 SULFATE ION × 4 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2.0M ammonium sulfate, 20mM Tris-HCl buffer (pH 7.5), 3% isopropanol
|
Resolution 1.25 Å R-free 0.152 |
| 9JIY Macrophage migration inhibitory factor S61H/Y100H mutant complexed with three Zinc ions (Zn3-MIF(S61H/Y100H)-L) Deposited 2024-09-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Mutation：S61H/Y100H Mutation：S61H/Y100H Mutation：S61H/Y100H | ZN ZINC ION × 3 CO3 CARBONATE ION × 1 SO4 SULFATE ION × 5 GOL GLYCEROL × 4 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；2.0M ammonium sulfate, 20mM Tris-HCl buffer (pH 7.5), 3% isopropanol
|
Resolution 1.20 Å R-free 0.174 |
| 9JIZ Macrophage migration inhibitory factor S61H/Y100H mutant complexed with three Zinc ions (Zn3-MIF(S61H/Y100H)) Deposited 2024-09-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Mutation：S61H/Y100H Mutation：S61H/Y100H Mutation：S61H/Y100H | ZN ZINC ION × 3 CO3 CARBONATE ION × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2.0M ammonium sulfate, 20mM Tris-HCl buffer (pH 7.5), 3% isopropanol
|
Resolution 1.30 Å R-free 0.167 |
| 9JJ0 Macrophage migration inhibitory factor Y100H mutant complexed with three Zinc ions (Zn3-MIF(Y100H)) Deposited 2024-09-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–115（115 aa）
Chain B
1–115（115 aa）
Chain C
1–115（115 aa）
|
Mutation：Y100H Mutation：Y100H Mutation：Y100H | ZN ZINC ION × 3 CL CHLORIDE ION × 3 CO3 CARBONATE ION × 1 SO4 SULFATE ION × 3 GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2.0M ammonium sulfate, 20mM Tris-HCl buffer (pH 7.5), 3% isopropanol
|
Resolution 1.25 Å R-free 0.146 |
117 other PDB entries and 131 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | MIF_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–114； UniProt 2–115 Author chain B； PDBConstruct 1–114； UniProt 2–115 Author chain C； PDBConstruct 1–114； UniProt 2–115 Author chain D； PDBConstruct 1–114； UniProt 2–115 Author chain E； PDBConstruct 1–114； UniProt 2–115 Author chain F； PDBConstruct 1–114； UniProt 2–115 |
