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4zrg

Visual arrestin mutant - R175E

Method: X-RAY DIFFRACTION Dmax: 93.1 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

S-arrestin

Bos taurus

UniProt P08168

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 1–404 Mutation：R175E CO2 CARBON DIOXIDE × 5 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；292.15 K；100mM Tris, 8-18% (w/v) PEG 4000 Resolution 2.70 Å R-free 0.241

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4ZRG Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1AYR ARRESTIN FROM BOVINE ROD OUTER SEGMENTS Deposited 1997-11-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–367（367 aa）
Chain B 1–367（367 aa）
Chain C 1–367（367 aa）
Chain D 1–367（367 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；pH 7.2
Resolution 3.30 Å R-free 0.316
1CF1 ARRESTIN FROM BOVINE ROD OUTER SEGMENTS Deposited 1999-03-23 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–404（404 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 2.80 Å R-free 0.244
1CF1 ARRESTIN FROM BOVINE ROD OUTER SEGMENTS Deposited 1999-03-23 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–404（404 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 2.80 Å R-free 0.244
1CF1 ARRESTIN FROM BOVINE ROD OUTER SEGMENTS Deposited 1999-03-23 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–404（404 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 2.80 Å R-free 0.244
1CF1 ARRESTIN FROM BOVINE ROD OUTER SEGMENTS Deposited 1999-03-23 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 1–404（404 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 2.80 Å R-free 0.244
1CF1 ARRESTIN FROM BOVINE ROD OUTER SEGMENTS Deposited 1999-03-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 1–404（404 aa）
Chain D 1–404（404 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；pH 7.5
Resolution 2.80 Å R-free 0.244
3UGU Crystal Structure of p44 (Splice Variant of Visual Arrestin) Deposited 2011-11-03 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–370（370 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；292.15 K；14% PEG 4000, 0.1M MES, 50 mM KH2PO4, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292.15K
Resolution 1.85 Å R-free 0.214
3UGX Crystal Structure of Visual Arrestin Deposited 2011-11-03 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–404（404 aa）
Not recorded PTD PENTANEDIAL × 8 NA SODIUM ION × 1 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；294.15 K；18mM PIPES,0.5M KCL,30% EG, 8% PEG 6000, 18% PEG200, 6% PEG 1000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K
Resolution 2.65 Å R-free 0.253
3UGX Crystal Structure of Visual Arrestin Deposited 2011-11-03 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–404（404 aa）
Not recorded PTD PENTANEDIAL × 2 NA SODIUM ION × 1 EDO 1,2-ETHANEDIOL × 1 IMD IMIDAZOLE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；294.15 K；18mM PIPES,0.5M KCL,30% EG, 8% PEG 6000, 18% PEG200, 6% PEG 1000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K
Resolution 2.65 Å R-free 0.253
3UGX Crystal Structure of Visual Arrestin Deposited 2011-11-03 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–404（404 aa）
Not recorded PTD PENTANEDIAL × 3 EDO 1,2-ETHANEDIOL × 3 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；294.15 K；18mM PIPES,0.5M KCL,30% EG, 8% PEG 6000, 18% PEG200, 6% PEG 1000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K
Resolution 2.65 Å R-free 0.253
3UGX Crystal Structure of Visual Arrestin Deposited 2011-11-03 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 1–404（404 aa）
Not recorded PTD PENTANEDIAL × 1 EDO 1,2-ETHANEDIOL × 1 IMD IMIDAZOLE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；294.15 K；18mM PIPES,0.5M KCL,30% EG, 8% PEG 6000, 18% PEG200, 6% PEG 1000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K
Resolution 2.65 Å R-free 0.253
4J2Q Crystal structure of C-terminally truncated arrestin reveals mechanism of arrestin activation Deposited 2013-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–369（369 aa） Fragment：UNP residues 1-369
Chain B 1–369（369 aa） Fragment：UNP residues 1-369
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；30% polyethylene glycol 200, 10 mM HEPES, 100 mM lithium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 3.00 Å R-free 0.279
4PXF Crystal structure of the active G-protein-coupled receptor opsin in complex with the finger-loop peptide derived from the full-length arrestin-1 Deposited 2014-03-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 67–77（11 aa）
Not recorded BOG octyl beta-D-glucopyranoside × 6 PLM PALMITIC ACID × 2 SO4 SULFATE ION × 2 ACT ACETATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；277 K；3.2 M (NH4)2SO4 in 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES), pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.75 Å R-free 0.251
7F1W X-ray crystal structure of visual arrestin complexed with inositol hexaphosphate Deposited 2021-06-10 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–404（404 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293.15 K；40 mM PIPES pH 7.2/0.6 M KCl/22% ethylene glycol/5.7% polyethylene glycol 6000/13% polyethylene glycol 200/4% polyethylene glycol 1000
Resolution 3.10 Å R-free 0.241
7F1W X-ray crystal structure of visual arrestin complexed with inositol hexaphosphate Deposited 2021-06-10 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–404（404 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293.15 K；40 mM PIPES pH 7.2/0.6 M KCl/22% ethylene glycol/5.7% polyethylene glycol 6000/13% polyethylene glycol 200/4% polyethylene glycol 1000
Resolution 3.10 Å R-free 0.241
7F1W X-ray crystal structure of visual arrestin complexed with inositol hexaphosphate Deposited 2021-06-10 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 1–404（404 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293.15 K；40 mM PIPES pH 7.2/0.6 M KCl/22% ethylene glycol/5.7% polyethylene glycol 6000/13% polyethylene glycol 200/4% polyethylene glycol 1000
Resolution 3.10 Å R-free 0.241
7F1W X-ray crystal structure of visual arrestin complexed with inositol hexaphosphate Deposited 2021-06-10 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–404（404 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293.15 K；40 mM PIPES pH 7.2/0.6 M KCl/22% ethylene glycol/5.7% polyethylene glycol 6000/13% polyethylene glycol 200/4% polyethylene glycol 1000
Resolution 3.10 Å R-free 0.241
7F1X X-ray crystal structure of visual arrestin complexed with inositol 1,4,5-triphosphate Deposited 2021-06-10 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–404（404 aa）
Not recorded I3P D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE × 1 PTD PENTANEDIAL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；40 mM PIPES pH 7.2/0.6 M KCl/22% ethylene glycol/5.7% polyethylene glycol 6000/13% polyethylene glycol 200/4% polyethylene glycol 1000
Resolution 3.00 Å R-free 0.239
7F1X X-ray crystal structure of visual arrestin complexed with inositol 1,4,5-triphosphate Deposited 2021-06-10 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–404（404 aa）
Not recorded I3P D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE × 1 PTD PENTANEDIAL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；40 mM PIPES pH 7.2/0.6 M KCl/22% ethylene glycol/5.7% polyethylene glycol 6000/13% polyethylene glycol 200/4% polyethylene glycol 1000
Resolution 3.00 Å R-free 0.239
7F1X X-ray crystal structure of visual arrestin complexed with inositol 1,4,5-triphosphate Deposited 2021-06-10 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–404（404 aa）
Not recorded I3P D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE × 1 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；40 mM PIPES pH 7.2/0.6 M KCl/22% ethylene glycol/5.7% polyethylene glycol 6000/13% polyethylene glycol 200/4% polyethylene glycol 1000
Resolution 3.00 Å R-free 0.239
7F1X X-ray crystal structure of visual arrestin complexed with inositol 1,4,5-triphosphate Deposited 2021-06-10 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 1–404（404 aa）
Not recorded I3P D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE × 1 PTD PENTANEDIAL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293.15 K；40 mM PIPES pH 7.2/0.6 M KCl/22% ethylene glycol/5.7% polyethylene glycol 6000/13% polyethylene glycol 200/4% polyethylene glycol 1000
Resolution 3.00 Å R-free 0.239
7JSM CRYSTAL STRUCTURE OF NATIVE BOVINE ARRESTIN 1 Deposited 2020-08-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–404（404 aa）
Chain B 1–404（404 aa）
Chain C 1–404（404 aa）
Chain D 1–404（404 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；281 K；0.1 M Bis-Tris propane, 35% 2-Ethoxyethanol, 0.001 M Magnesium acetate
Resolution 2.50 Å R-free 0.233
7JTB CRYSTAL STRUCTURE OF NATIVE BOVINE ARRESTIN 1 IN COMPLEX WITH INOSITOL HEXAKISPHOSPHATE Deposited 2020-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–404（404 aa）
Chain B 1–404（404 aa）
Chain C 1–404（404 aa）
Chain D 1–404（404 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；281 K；0.1 M Bis-Tris propane, 35% 2-Ethoxyethanol, 0.001 M Magnesium acetate, 0.0009 M Phytic acid sodium salt
Resolution 2.60 Å R-free 0.238
7JXA CRYSTAL STRUCTURE OF NATIVE BOVINE ARRESTIN 1 IN COMPLEX WITH INOSITOL 1,4,5-TRIPHOSPHATE Deposited 2020-08-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–404（404 aa）
Chain B 1–404（404 aa）
Chain C 1–404（404 aa）
Chain D 1–404（404 aa）
Not recorded I3P D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE × 4 NA SODIUM ION × 3 ETX 2-ETHOXYETHANOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；281 K；0.1 M Bis-Tris propane, 35% 2-Ethoxyethanol, 0.0025 M NaCl; 0.005 M IP3 overnight soak
Resolution 2.40 Å R-free 0.249
7MOR CRYSTAL STRUCTURE OF NATIVE BOVINE ARRESTIN 1 IN COMPLEX WITH 5-METHYLENEBIPHOSPHONATE INOSITOL PENTAKISPHAOPHATE (5-PCP IP5) Deposited 2021-05-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–404（404 aa）
Chain B 1–404（404 aa）
Chain C 1–404（404 aa）
Chain D 1–404（404 aa）
Not recorded 5A3 Methylenebisphosphonate inositol pentakisphosphate × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；281 K；0.1 M Bis-Tris propane, 35% 2-Ethoxyethanol, 0.001 M Magnesium acetate, 10 mM 5-Methylenebisphosphonate inositol pentakisphosphate
Resolution 2.80 Å R-free 0.246
7MP0 CRYSTAL STRUCTURE OF NATIVE BOVINE ARRESTIN 1 IN COMPLEX WITH 1D-MYO-INOSITOL 5-DIPHOSPHATE PENTAKISPHOSPHATE (5-PP IP5) Deposited 2021-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–404（404 aa）
Chain B 1–404（404 aa）
Chain C 1–404（404 aa）
Chain D 1–404（404 aa）
Not recorded ETX 2-ETHOXYETHANOL × 1 I7P (1r,2R,3S,4s,5R,6S)-2,3,4,5,6-pentakis(phosphonooxy)cyclohexyl trihydrogen diphosphate × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；281 K；0.1 M Bis-tris propane pH 7, 35% 2-Ethoxyethanol, 0.001 M Magnesium acetate, 5 mM 1D-myo-inositol 5-diphosphate pentakisphosphate
Resolution 2.60 Å R-free 0.248
7MP1 CRYSTAL STRUCTURE OF NATIVE BOVINE ARRESTIN 1 IN COMPLEX WITH 1,5-DI-METHYLENEBISPHOSPHONATE INOSITOL TETRAKISPHOSPHATE (1,5-PCP-IP4) Deposited 2021-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–404（404 aa）
Chain B 1–404（404 aa）
Chain C 1–404（404 aa）
Chain D 1–404（404 aa）
Not recorded 4WZ {[(1R,3S,4S,5R,6S)-2,4,5,6-tetrakis(phosphonooxy)cyclohexane-1,3-diyl]bis[oxy(hydroxyphosphoryl)methanediyl]}bis(phosphonic acid) × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；281 K；0.1 M Bis-Tris propane pH 7.0, 35% 2-Ethoxyethanol, 0.001 M Magnesium acetate, 5 mM 1,5-DI-METHYLENEBISPHOSPHONATE INOSITOL TETRAKISPHOSPHATE (1,5-PCP-IP4)
Resolution 2.66 Å R-free 0.248
7MP2 CRYSTAL STRUCTURE OF NATIVE BOVINE ARRESTIN 1 IN COMPLEX WITH 1D-MYO-INOSITOL 1,5-BISDIPHOSPHATE TETRAKISPHOSPHATE (1,5-PP IP4) Deposited 2021-05-04 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–404（404 aa）
Chain B 1–404（404 aa）
Chain C 1–404（404 aa）
Chain D 1–404（404 aa）
Not recorded I8P (1R,3S,4R,5S,6R)-2,4,5,6-tetrakis(phosphonooxy)cyclohexane-1,3-diyl bis[trihydrogen (diphosphate)] × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；281 K；0.1 M Bis-Tris propane, 35% 2-Ethoxyethanol, 0.001 mM Magnesium acetate, 5 mM 1D-MYO-INOSITOL 1,5-BISDIPHOSPHATE TETRAKISPHOSPHATE
Resolution 3.00 Å R-free 0.245
9C6E High-resolution structure of bovine (3-367)Arrestin-1 in a pre-activated conformation Deposited 2024-06-07 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 3–367（365 aa）
Not recorded GOL GLYCEROL × 1 GOA GLYCOLIC ACID × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.1 M imidazole, 0.1 M MES pH 6.5, 10% PEG 4000, 20% glycerol, 20 mM sodium formate; 20 mM ammonium acetate; 20 mM sodium citrate tribasic dihydrate; 20 mM potassium sodium tartrate tetrahydrate; 20 mM sodium oxamate
Resolution 1.40 Å R-free 0.180

16 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ARRS_BOVIN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 9–412； UniProt 1–404

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 4zrg

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 4zrg
Download Download
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2. Structure Basics 2. Structure Basics

Entry ID entry_id4zrg
Deposition date deposition_date2015-05-12
Structure title titleVisual arrestin mutant - R175E
Keywords keywordsarrestin fold, signal termination, GPCR, outer segment, signaling protein; SIGNALING PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier26.07 Å
Radius of gyration Rg (electron density) rg_electron25.94 Å
Forward intensity I(0) i022015900.00
Molecular weight molecular_weight37205.0 kDa
Excluded volume excluded_volume47214 ų
Envelope volume envelope_volume58490 ų
Hydration-shell volume shell_volume21200 ų
Envelope diameter envelope_diameter99.0 Å
Shell Rg shell_rg29.95 Å
Envelope Rg envelope_rg26.50 Å
Shape Rg shape_rg25.93 Å
Total Rg total_rg26.48 Å
Total atoms total_atoms2623
Residues n_residues343
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax93.1 Å
Rg (real space) rg_real26.46 Å
Rg uncertainty (real space) rg_real_error0.91 Å
I(0) (real space) i0_real2.2020e+07
I(0) uncertainty (real space) i0_real_error3.4560e+05
Rg (reciprocal space) rg_reciprocal26.34 Å
I(0) (reciprocal space) i0_reciprocal22010000.0000
Solution quality estimate total_estimate0.7919
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary22.9 Å
Skewness Skewness skewness0.727
Kurtosis Kurtosis kurtosis0.161
Angular range angular_range— – 0.3050 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha6198000.0000
Real-space data points n_real_points62
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.635; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.585; Smooth: 0.799
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 3 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd4zrga1
Class classb — All beta proteins
Fold Fold foldb.1 — Immunoglobulin-like beta-sandwich
Superfamily Superfamily superfamilyb.1.18 — E set domains
Family Family familyb.1.18.11 — Arrestin/Vps26-like

CATH v4.4 (2 domains)

Domain ID domain_id4zrgA01
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily840 —
Domain ID domain_id4zrgA02
Class class2 — Mainly Beta
Architecture architecture60 — Sandwich
Topology topology40 — Immunoglobulin-like
Homologous superfamily homologous superfamily640 —
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8. Citations (1)

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9. Files and Curves (10)