Carbonic anhydrase 2
Bos taurus
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain X； UniProt 4–260 | Fragment：UNP residues 4-260 Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；277.15 K；1.6M ammonium sulfate, tris | Resolution 1.54 Å R-free 0.239 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 5EZT | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1G6V Complex of the camelid heavy-chain antibody fragment CAB-CA05 with bovine carbonic anhydrase Deposited 2000-11-08 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
0–260（261 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；PEG 8000, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.50 Å R-free 0.276 |
| 1V9E Crystal Structure Analysis of Bovine Carbonic Anhydrase II Deposited 2004-01-26 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–259（259 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 7.5；277 K；50mM Tris-HCl, 2.4M Ammonium sulfate, pH 7.5, SMALL TUBES, temperature 277K
|
Resolution 1.95 Å R-free 0.298 |
| 1V9E Crystal Structure Analysis of Bovine Carbonic Anhydrase II Deposited 2004-01-26 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–259（259 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 7.5；277 K；50mM Tris-HCl, 2.4M Ammonium sulfate, pH 7.5, SMALL TUBES, temperature 277K
|
Resolution 1.95 Å R-free 0.298 |
| 1V9I Crystal Structure Analysis of the site specific mutant (Q253C) of bovine carbonic anhydrase II Deposited 2004-01-26 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–259（259 aa）
|
Mutation：Q253C | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
SMALL TUBES；pH 7.5；277 K；50mM Tris-HCl, 3mM NaN3, 2.3M Ammonium Sulfate, pH 7.5, SMALL TUBES, temperature 277K
|
Resolution 2.95 Å R-free 0.346 |
| 23OM Crystal Structure Analysis of Bovine Carbonic Anhydrase II to 5-(2-morpholin-4-ylcarbonyl-1,3-oxazol-5-yl)thiophene-2-sulfonamide. Deposited 2026-02-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–260（259 aa）
|
Not recorded | 8V5 5-(2-morpholin-4-ylcarbonyl-1,3-oxazol-5-yl)thiophene-2-sulfonamide × 1 BOG octyl beta-D-glucopyranoside × 1 GOL GLYCEROL × 3 ZN ZINC ION × 1 ACT ACETATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；2M ammonium sulfate, 0.1M Tris base Buffer
|
Resolution 1.70 Å R-free 0.250 |
| 23OM Crystal Structure Analysis of Bovine Carbonic Anhydrase II to 5-(2-morpholin-4-ylcarbonyl-1,3-oxazol-5-yl)thiophene-2-sulfonamide. Deposited 2026-02-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
2–260（259 aa）
|
Not recorded | 8V5 5-(2-morpholin-4-ylcarbonyl-1,3-oxazol-5-yl)thiophene-2-sulfonamide × 1 BOG octyl beta-D-glucopyranoside × 1 GOL GLYCEROL × 3 ZN ZINC ION × 1 ACT ACETATE ION × 1 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；2M ammonium sulfate, 0.1M Tris base Buffer
|
Resolution 1.70 Å R-free 0.250 |
| 23ON Crystal Structure Analysis of Bovine Carbonic Anhydrase II to 4-2-methyl-1,3-oxazol-5-ylbenzenesulfonamide Deposited 2026-02-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–260（259 aa）
|
Not recorded | 8V8 4-(2-methyl-1,3-oxazol-5-yl)benzenesulfonamide × 1 GOL GLYCEROL × 3 ACT ACETATE ION × 1 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；2M ammonium sulfate, 0.1M Tris-base Buffer
|
Resolution 1.50 Å R-free 0.200 |
| 23ON Crystal Structure Analysis of Bovine Carbonic Anhydrase II to 4-2-methyl-1,3-oxazol-5-ylbenzenesulfonamide Deposited 2026-02-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
2–260（259 aa）
|
Not recorded | 8V8 4-(2-methyl-1,3-oxazol-5-yl)benzenesulfonamide × 1 GOL GLYCEROL × 3 ACT ACETATE ION × 2 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；2M ammonium sulfate, 0.1M Tris-base Buffer
|
Resolution 1.50 Å R-free 0.200 |
| 4CNR Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture Deposited 2014-01-24 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–260（260 aa）
|
Mutation：YES | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.5；281 K；10 MG/ML PROTEIN WITH 30% PEG 1500 AT 8C, pH 5.5
|
Resolution 2.29 Å R-free 0.236 |
| 4CNR Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture Deposited 2014-01-24 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–260（260 aa）
|
Mutation：YES | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.5；281 K；10 MG/ML PROTEIN WITH 30% PEG 1500 AT 8C, pH 5.5
|
Resolution 2.29 Å R-free 0.236 |
| 4CNR Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture Deposited 2014-01-24 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–260（260 aa）
|
Mutation：YES | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.5；281 K；10 MG/ML PROTEIN WITH 30% PEG 1500 AT 8C, pH 5.5
|
Resolution 2.29 Å R-free 0.236 |
| 4CNR Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture Deposited 2014-01-24 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
1–260（260 aa）
|
Mutation：YES | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.5；281 K；10 MG/ML PROTEIN WITH 30% PEG 1500 AT 8C, pH 5.5
|
Resolution 2.29 Å R-free 0.236 |
| 4CNV Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture Deposited 2014-01-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–260（260 aa）
|
Mutation：YES | ZN ZINC ION × 1 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6；PROTEIN WAS AT 10 MG/ML; THE RESERVOIR SOLUTION WAS 5% PEG 1000, 30% PEG 600, 10% GLYCEROL, 100 MM SODIUM MES BUFFER AT PH 6. SEEDS OF THE MUT1 FORM WERE USED TO OBTAIN THESE CRYSTALS.
|
Resolution 1.62 Å R-free 0.182 |
| 4CNW Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture Deposited 2014-01-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–260（260 aa）
|
Mutation：YES | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；PROTEIN WAS AT 10 MG/ML. THE RESERVOIR CONDITIONS WERE: 25% PEG MME 2000, 200 MM CALCIUM ACETATE, 50 MM TRIS BUFFER PH 8.0. THE CRYSTALS WERE OBTAINED THROUGH CROSS SEEDING FROM A DIFFERENT MUTANT CA-II CRYSTAL.
|
Resolution 2.03 Å R-free 0.259 |
| 4CNW Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture Deposited 2014-01-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–260（260 aa）
|
Mutation：YES | ZN ZINC ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；PROTEIN WAS AT 10 MG/ML. THE RESERVOIR CONDITIONS WERE: 25% PEG MME 2000, 200 MM CALCIUM ACETATE, 50 MM TRIS BUFFER PH 8.0. THE CRYSTALS WERE OBTAINED THROUGH CROSS SEEDING FROM A DIFFERENT MUTANT CA-II CRYSTAL.
|
Resolution 2.03 Å R-free 0.259 |
| 4CNX Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture Deposited 2014-01-25 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–260（260 aa）
|
Mutation：YES | ZN ZINC ION × 1 PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.5；281 K；PROTEIN WAS AT 10 MG/ML. THE RESERVOIR CONDITIONS WERE: 40% PEG 600, 100 MM SODIUM CITRATE AT PH 5.5 SET UP AT 8C. CROSS SEEDING FROM OTHER MUTANT CA-II CRYSTALS WAS USED TO OBTAIN THESE CRYSTALS.
|
Resolution 1.23 Å R-free 0.147 |
| 5A25 Rational engineering of a mesophilic carbonic anhydrase to an extreme halotolerant biocatalyst Deposited 2015-05-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–260（260 aa）
|
Not recorded | ZN ZINC ION × 1 GOL GLYCEROL × 1 NA SODIUM ION × 2 BOG octyl beta-D-glucopyranoside × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.2；150 NL 20 MG/ML PROTEIN PLUS 150 NL RESERVOIR CONSISTING OF 50 MM TRIS PH 8.2, 2.3 M AMMONIUM SULFATE, 0.023 (W/V) N-OCTYL-B-D-GLUCOPYRANOSIDE
|
Resolution 1.90 Å R-free 0.197 |
| 5A25 Rational engineering of a mesophilic carbonic anhydrase to an extreme halotolerant biocatalyst Deposited 2015-05-12 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–260（260 aa）
|
Not recorded | ZN ZINC ION × 1 NA SODIUM ION × 2 BOG octyl beta-D-glucopyranoside × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.2；150 NL 20 MG/ML PROTEIN PLUS 150 NL RESERVOIR CONSISTING OF 50 MM TRIS PH 8.2, 2.3 M AMMONIUM SULFATE, 0.023 (W/V) N-OCTYL-B-D-GLUCOPYRANOSIDE
|
Resolution 1.90 Å R-free 0.197 |
| 6SKS Crystal structure of bovine carbonic anhydrase II in complex with a benzenesulfonamide-based ligand (SH1) Deposited 2019-08-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–260（260 aa）
|
Not recorded | CU COPPER (II) ION × 4 ZN ZINC ION × 1 GOL GLYCEROL × 2 E68 ~{N}-[2-[2-(1~{H}-imidazol-4-yl)ethylamino]-2-oxidanylidene-ethyl]-4-sulfamoyl-benzamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；100mM HEPES pH7.5, 14% PEG3350, 20% Glycerol, 5mM CuCl2
|
Resolution 1.75 Å R-free 0.194 |
| 6SKT Crystal structure of bovine carbonic anhydrase II in complex with a benzenesulfonamide-based ligand (SH0) Deposited 2019-08-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–260（260 aa）
|
Not recorded | CU COPPER (II) ION × 2 ZN ZINC ION × 1 GOL GLYCEROL × 1 E6B ~{N}-[2-(1~{H}-imidazol-4-yl)ethyl]-4-sulfamoyl-benzamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；100mM HEPES pH7.5, 14% PEG3350, 20% Glycerol, 5mM CuCl2
|
Resolution 1.90 Å R-free 0.204 |
| 6SKV Crystal structure of bovine carbonic anhydrase II in complex with a benzenesulfonamide-based ligand (SH2) Deposited 2019-08-16 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–260（260 aa）
|
Not recorded | ZN ZINC ION × 1 CU COPPER (II) ION × 3 GOL GLYCEROL × 2 E5Z ~{N}-[3-[(~{E})-2-(1~{H}-imidazol-5-yl)ethylideneamino]-3-oxidanylidene-propyl]-4-sulfamoyl-benzamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；100mM HEPES pH7.5, 14% PEG3350, 20% Glycerol, 5mM CuCl2.
|
Resolution 1.75 Å R-free 0.189 |
13 other PDB entries and 21 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | CAH2_BOVIN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain X； PDBConstruct 1–257； UniProt 4–260 |
