5mi3

Structure of phosphorylated translation elongation factor EF-Tu from E. coli

Method: X-RAY DIFFRACTION
▼

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Elongation factor Tu 1

Escherichia coli (strain K12)

UniProt P0CE47

当前结构中的状态

Assembly 物理组成 蛋白状态 分子拷贝数 共同组分 数据核对
1 蛋白单体 单体 蛋白 1 MAGNESIUM ION × 1 GUANOSINE-5'-DIPHOSPHATE × 1 water × 1 与蛋白数一致
2 蛋白单体 单体 蛋白 1 MAGNESIUM ION × 1 GUANOSINE-5'-DIPHOSPHATE × 1 water × 1 与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据
UniProt名称 EFTU1_ECOLI
Isoform —
PDB实体 1
链与序列区间 作者链 A; PDB构建体 10–402; UniProt 2–394 作者链 B; PDB构建体 10–402; UniProt 2–394

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

▼

2. 结构基本信息 2. Structure Basics

条目编号 entry_id5mi3
沉积日期 deposition_date2016-11-27
结构标题 titleStructure of phosphorylated translation elongation factor EF-Tu from E. coli
关键词 keywords;translation elongation, EF-Tu, phosphorylation, nucleotide binding, protein dynamics, conformational cycle, toxin-antitoxin, TOXIN, hydrolase ;; HYDROLASE
实验方法 methodX-RAY DIFFRACTION
▼

3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

5mi3__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

5mi3__assembly_2__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 个绘图点;横轴和纵轴均采用对数尺度。

5mi3__assembly_2__model_1 | P(r) · Pending

新版 assembly/model P(r) 尚未计算 此处仅保留位置,不展示旧数据 r (Angstrom) P(r)
P(r) 将按相同 assembly/model 分别计算和展示。
Rg(Guinier)24.29 Å
Rg(电子密度)23.57 Å
总 Rg24.38 Å
原子数2912
残基数385
排除体积51682 ų
最大 q0.500 Å⁻¹
Assembly Model 结构单元 聚集说明 状态 CRYSOL 操作
1 1 5mi3__assembly_1__model_1 monomeric (1) 成功 4.1.3-1-20251215 (887e7ef) 查看 下载
2 1 5mi3__assembly_2__model_1 monomeric (1) 成功 4.1.3-1-20251215 (887e7ef) 查看 下载
▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合物信息 Entities & Polymers (4)

▼

6. 折叠分类 (SCOP + CATH) 12 domains

SCOP 2.08 (6 domains)

结构域编号 domain_idd5mi3a1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.8 — G proteins
结构域编号 domain_idd5mi3a2
类 Class classb — All beta proteins
折叠类型 Fold foldb.43 — Reductase/isomerase/elongation factor common domain
超家族 Superfamily superfamilyb.43.3 — Translation proteins
家族 Family familyb.43.3.0 — automated matches
结构域编号 domain_idd5mi3a3
类 Class classb — All beta proteins
折叠类型 Fold foldb.44 — Elongation factor/aminomethyltransferase common domain
超家族 Superfamily superfamilyb.44.1 — EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain
家族 Family familyb.44.1.0 — automated matches
结构域编号 domain_idd5mi3b1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.8 — G proteins
结构域编号 domain_idd5mi3b2
类 Class classb — All beta proteins
折叠类型 Fold foldb.43 — Reductase/isomerase/elongation factor common domain
超家族 Superfamily superfamilyb.43.3 — Translation proteins
家族 Family familyb.43.3.0 — automated matches
结构域编号 domain_idd5mi3b3
类 Class classb — All beta proteins
折叠类型 Fold foldb.44 — Elongation factor/aminomethyltransferase common domain
超家族 Superfamily superfamilyb.44.1 — EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain
家族 Family familyb.44.1.0 — automated matches

CATH v4.4 (6 domains)

结构域编号 domain_id5mi3A01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id5mi3A02
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology30 — Elongation Factor Tu (Ef-tu); domain 3
同源超家族 H-superfamily homologous superfamily10 — Translation factors
结构域编号 domain_id5mi3A03
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology30 — Elongation Factor Tu (Ef-tu); domain 3
同源超家族 H-superfamily homologous superfamily10 — Translation factors
结构域编号 domain_id5mi3B01
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id5mi3B02
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology30 — Elongation Factor Tu (Ef-tu); domain 3
同源超家族 H-superfamily homologous superfamily10 — Translation factors
结构域编号 domain_id5mi3B03
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology30 — Elongation Factor Tu (Ef-tu); domain 3
同源超家族 H-superfamily homologous superfamily10 — Translation factors
▶

7. 引用文献 (1)