SAXSdb — 5txe

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1. Protein Identity and Related Structures Protein Identity & Related Structures

AtxE2

Asticcacaulis excentricus (strain ATCC 15261 / DSM 4724 / VKM B-1370 / CB 48)

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 2	Astexin3-dC4 × 1 （E8RUP8） water × 2	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 2	Astexin3-dC4 × 1 （E8RUP8） water × 2	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 4	Astexin3-dC4 × 2 （E8RUP8） water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	E8RUP5_ASTEC
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–695； UniProt 1–695 Author chain B； PDBConstruct 1–695； UniProt 1–695

Astexin3-dC4

Asticcacaulis excentricus (strain ATCC 15261 / DSM 4724 / VKM B-1370 / CB 48)

UniProt E8RUP8

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 2	AtxE2 × 1 （E8RUP5） water × 2	Consistent with protein count
2	Protein heterocomplex	Heteromer	Protein 2	AtxE2 × 1 （E8RUP5） water × 2	Consistent with protein count
3	Protein heterocomplex	Heteromer	Protein 4	AtxE2 × 2 （E8RUP5） water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	E8RUP8_ASTEC
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain C； PDBConstruct 1–24； UniProt 26–49 Author chain D； PDBConstruct 1–24； UniProt 26–49

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	5txe
Deposition date deposition_date	2016-11-16
Structure title title	AtxE2 Isopeptidase - S527A Variant with Astexin3-dC4 Bound
Keywords keywords	Isopeptidase, Lasso peptide, Natural Product, HYDROLASE; HYDROLASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5txe__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5txe__assembly_2__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

5txe__assembly_2__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	26.88 Å
Rg (electron density)	25.72 Å
Total Rg	26.62 Å
Atom count	5282
Residues	670
Excluded volume	93134 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`5txe__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`5txe__assembly_2__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`5txe__assembly_3__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	AtxE2
Molecular weight formula_weight	78683.8 kDa
Mutation mutation	S527A
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	11501

MRSSKIRCPGAIRVGTLVTAFGCLPHVAFAAAREAPPVTPEVLVRLADIGTMSASETTPLLSLSPDGRYVAFQVRQADPVTNLNVFRMVVKATDGATDAIDVDVGGEYLFWTIPSWGYARNAPSGANLTIQPRWSPSGTHLAYLRQDQGRVRVWRASVKGEGASPVIEDAYDIEDVQWLDDNTLIYSGRPGFVEAEAEIEREGRRGWVYDERFHPLTGARPRVLEPISIVYQVLDLKTGTRRAATPTEVARLREKPDPLRAMVGRTTFSVSRTDPQNINAPTTLVARRGEGEPVRCDEEACQNITRMWGDETANVLYFLRREGWASNEMALYRMPADALKPVRIWHATGLLQGCERQAKRLICAQESALQPRRLVTLNLTSGQMSPLYDPNPDLSRYRLPKVERLTLRNRNGIEVFSDLVLPPDYQLGTRLPLVIVQYSSRGFLRGGTGDENPILPLATAGFAVLSFHSPRSEASYQRFTSPIAQSKAEYSNWRNRWNILHTLEDLIDDLDRRGVIDPARVGLTGLADGATTVHFGLINSHRFAAAVTSSCCTDSFTASVMNGPRISGALKAYGIETDQADDGPFWAATSFVVNASRLDTPLLIQSADEEYLGALPGFTALQQARKPVELIIYPNEHHVKWQPAHRLAVYNRTIDWFRFWLMDQSDPAPDKAAQYDRWRALRALRQKSPSPTPAPGSRSHHHHHH

Source Source

Source organism organism	Asticcacaulis excentricus (strain ATCC 15261 / DSM 4724 / VKM B-1370 / CB 48)
NCBI taxonomy ID ncbi_taxonomy_id	573065
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	Astexin3-dC4
Molecular weight formula_weight	2542.8 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,D

GPTPMVGLDSVSGQYWDQHAPLAD

Source Source

Source organism organism	Asticcacaulis excentricus (strain ATCC 15261 / DSM 4724 / VKM B-1370 / CB 48)
NCBI taxonomy ID ncbi_taxonomy_id	573065
Host organism host_organism	Escherichia coli

Entity 3 Entity 3 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	910

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Unit-cell length a length_a	151.253 Å
Unit-cell length b length_b	202.854 Å
Unit-cell length c length_c	110.399 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	16

Space group space_group	C 2 2 2
Space-group number int_tables_number	21
Crystal system crystal_system	—

Resolution resolution	2.200 Å
Low-resolution limit resolution_low	29.817 Å
R factor r_factor	0.1565
R_work r_work	0.1545
R_free r_free	0.1976

AtxE2 Isopeptidase - S527A Variant with Astexin3-dC4 Bound

1. Protein Identity and Related Structures Protein Identity & Related Structures

AtxE2

State in the Current Structure

Other States of the Same Protein in the Database

Astexin3-dC4

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

5txe__assembly_2__model_1

5txe__assembly_2__model_1 | I(q)

5txe__assembly_2__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 water

7. Citations (1)

AtxE2 Isopeptidase - S527A Variant with Astexin3-dC4 Bound

1. Protein Identity and Related Structures Protein Identity & Related Structures

AtxE2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Astexin3-dC4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

5txe__assembly_2__model_1

5txe__assembly_2__model_1 | I(q)

5txe__assembly_2__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

Entity 3 Entity 3 water

7. Citations (1)