6ezn

Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 8 其他Polymer 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	OST1_YEAST
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–476； UniProt 1–476

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2

OrganismNot specified

UniProt P46964

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 8 其他Polymer 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	OST2_YEAST
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–130； UniProt 1–130

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3

OrganismNot specified

UniProt P48439

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 8 其他Polymer 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	OST3_YEAST
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–350； UniProt 1–350

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4

OrganismNot specified

UniProt Q99380

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 8 其他Polymer 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	OST4_YEAST
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–36； UniProt 1–36

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5

OrganismNot specified

UniProt Q92316

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 8 其他Polymer 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	OST5_YEAST
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–86； UniProt 1–86

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3

OrganismNot specified

UniProt P39007

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 8 其他Polymer 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	STT3_YEAST
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–718； UniProt 1–718

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

OrganismNot specified

UniProt P33767

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 8 其他Polymer 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	OSTB_YEAST
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–430； UniProt 1–430

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1

OrganismNot specified

UniProt Q02795

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 8 其他Polymer 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	OSTD_YEAST
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain H； PDBConstruct 1–286； UniProt 1–286

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	6ezn
Deposition date deposition_date	2017-11-16
Structure title title	Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex
Keywords keywords	OST complex, oligosaccharyltransferase, N-linked glycosylation, yeast, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

6ezn__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

6ezn__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

6ezn__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	46.41 Å
Rg (electron density)	46.21 Å
Total Rg	46.42 Å
Atom count	17008
Residues	2090
Excluded volume	307390 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`6ezn__assembly_1__model_1`	octameric （8）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (14)

Entity 1 Entity 1 polymer

Description description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Molecular weight formula_weight	54116.5 kDa
EC 编号 ec_number	2.4.99.18
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	17008

MRQVWFSWIVGLFLCFFNVSSAAQYEPPATWENVDYKRTIDVSNAYISETIEITIKNIASEPATEYFTAFESGIFSKVSFFSAYFTNEATFLNSQLLANSTTAPGDDGESEIRYGIIQFPNAISPQEEVSLVIKSFYNTVGIPYPEHVGMSEEQHLLWETNRLPLSAYDTKKASFTLIGSSSFEEYHPPNDESLLGKANGNSFEFGPWEDIPRFSSNETLAIVYSHNAPLNQVVNLRRDIWLSHWASTIQFEEYYELTNKAAKLSKGFSRLELMKQIQTQNMRQTHFVTVLDMLLPEGATDHYFTDLVGLVSTSHAERDHFFIRPRFPIFGGWNYNFTVGWTNKLSDFLHVSSGSDEKFVASIPILNGPPDTVYDNVELSVFLPEGAEIFDIDSPVPFTNVSIETQKSYFDLNKGHVKLTFSYRNLISQVANGQVLIKYDYPKSSFFKKPLSIACYIFTALMGVFVLKTLNMNVTN

Entity 2 Entity 2 polymer

Description description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2
Molecular weight formula_weight	14712.5 kDa
EC 编号 ec_number	2.4.99.18
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MAKAPKANTPKVTSTSSAVLTDFQETFKTSKRAYFAQIEKYPKLKLIDTFCFFLVLLGVIQCTFIILIRDNFPFNAFLAGFIICVGQFVLLMSLRLQLCNSFPGISKNRAFAEFIVASLILHFVCLHFIN

Entity 3 Entity 3 polymer

Description description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3
Molecular weight formula_weight	39518.2 kDa
EC 编号 ec_number	2.4.99.18
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MNWLFLVSLVFFCGVSTHPALAMSSNRLLKLANKSPKKIIPLKDSSFENILAPPHENAYIVALFTATAPEIGCSLCLELESEYDTIVASWFDDHPDAKSSNSDTSIFFTKVNLEDPSKTIPKAFQFFQLNNVPRLFIFKPNSPSILDHSVISISTDTGSERMKQIIQAIKQFSQVNDFSLHLPMDWTPIITSTIITFITVLLFKKQSKLMFSIISSRIIWATLSTFFIICMISAYMFNQIRNTQLAGVGPKGEVMYFLPNEFQHQFAIETQVMVLIYGTLAALVVVLVKGIQFLRSHLYPETKKAYFIDAILASFCALFIYVFFAALTTVFTIKSPAYPFPLLRLSAPFK

Entity 4 Entity 4 polymer

Description description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4
Molecular weight formula_weight	3986.7 kDa
EC 编号 ec_number	2.4.99.18
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MISDEQLNSLAITFGIVMMTLIVIYHAVDSTMSPKN

Entity 5 Entity 5 polymer

Description description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5
Molecular weight formula_weight	9525.1 kDa
EC 编号 ec_number	2.4.99.18
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MTYEQLYKEFHSSKSFQPFIHLDTQPKFAICGLIVTLAVLSSALFAVGSKSSYIKKLFFYTILSVIGSLFAGLTTVFASNSFGVYV

Entity 6 Entity 6 polymer

Description description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Molecular weight formula_weight	81604.5 kDa
EC 编号 ec_number	2.4.99.18
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MGSDRSCVLSVFQTILKLVIFVAIFGAAISSRLFAVIKFESIIHEFDPWFNYRATKYLVNNSFYKFLNWFDDRTWYPLGRVTGGTLYPGLMTTSAFIWHALRNWLGLPIDIRNVCVLFAPLFSGVTAWATYEFTKEIKDASAGLLAAGFIAIVPGYISRSVAGSYDNEAIAITLLMVTFMFWIKAQKTGSIMHATCAALFYFYMVSAWGGYVFITNLIPLHVFLLILMGRYSSKLYSAYTTWYAIGTVASMQIPFVGFLPIRSNDHMAALGVFGLIQIVAFGDFVKGQISTAKFKVIMMVSLFLILVLGVVGLSALTYMGLIAPWTGRFYSLWDTNYAKIHIPIIASVSEHQPVSWPAFFFDTHFLIWLFPAGVFLLFLDLKDEHVFVIAYSVLCSYFAGVMVRLMLTLTPVICVSAAVALSKIFDIYLDFKTSDRKYAIKPAALLAKLIVSGSFIFYLYLFVFHSTWVTRTAYSSPSVVLPSQTPDGKLALIDDFREAYYWLRMNSDEDSKVAAWWDYGYQIGGMADRTTLVDNNTWNNTHIAIVGKAMASPEEKSYEILKEHDVDYVLVIFGGLIGFGGDDINKFLWMIRISEGIWPEEIKERDFYTAEGEYRVDARASETMRNSLLYKMSYKDFPQLFNGGQATDRVRQQMITPLDVPPLDYFDEVFTSENWMVRIYQLKKDDAQGRTLRDVGELTRSSTKTRRSIKRPELGLRV

Entity 7 Entity 7 polymer

Description description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1
Molecular weight formula_weight	49444.4 kDa
EC 编号 ec_number	2.4.99.18
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MRTDWNFFFCILLQAIFVVGTQTSRTLVLYDQSTEPLEEYSVYLKDLEQRNYKLEYLDINSTSTTVDLYDKEQRLFDNIIVFPTKGGKNLARQIPVKQLIKFFENEGNILCMSSPGAVPNTIRLFLNELGIYPSPKGHVIRDYFSPSSEELVVSSNHLLNKYVYNARKSEDFVFGESSAALLENREQIVPILNAPRTSFTESKGKCNSWTSGSQGFLVVGFQNLNNARLVWIGSSDFLKNKNQDSNQEFAKELLKWTFNEKSVIKSVHAVHSHADGTSYDEEPYKIKDKVIYSVGFSEWNGEEWLPHIADDIQFELRQVDPYYRLTLSPSGNDSETQYYTTGEFILPDRHGVFTFLTDYRKIGLSFTTDKDVKAIRHLANDEYPRSWEISNSWVYISAICGVIVAWIFFVVSFVTTSSVGKKLETFKKTN

Entity 8 Entity 8 polymer

Description description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1
Molecular weight formula_weight	31682.8 kDa
EC 编号 ec_number	2.4.99.18
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MQFFKTLAALVSCISFVLAYVAQDVHVSFPSTAGKSRVMIGKVEPRIGIDETVPTTITVEDPNEVIQVNFAIESTNKPFQNTLLIGLPNKNLEMAFEPEIKDNGKLSMYKYRIDLAKLDAALLQEASRSPEPIKATLILASSTAKPKENLFREILQLNLNFDVDHSDSSLVDKFGIKPEIHHIFHAEPKRVAKPIAVIFVLIIFITILSLIVTWLNSCAAAFNNIPTGVTAVYFLGFIATIVGFEVIFARYYLGTSIFETLFSSLYLGAPGLLTSTKFLRSFGQTI

Entity 9 Entity 9 branched

Description description	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
Molecular weight formula_weight	586.5 kDa
Source method src_method	man
Copy count entity_count	1

Entity 10 Entity 10 branched

Description description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
Molecular weight formula_weight	424.4 kDa
Source method src_method	man
Copy count entity_count	2

Entity 11 Entity 11 branched

Description description	;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
Molecular weight formula_weight	1397.2 kDa
Source method src_method	man
Copy count entity_count	1

Entity 12 Entity 12 non-polymer

Description description	1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE
Molecular weight formula_weight	758.1 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 13 Entity 13 non-polymer

Description description	PHOSPHATIDYLETHANOLAMINE
Molecular weight formula_weight	734.0 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 14 Entity 14 non-polymer

Description description	2-acetamido-2-deoxy-beta-D-glucopyranose
Molecular weight formula_weight	221.2 kDa
Source method src_method	man
Copy count entity_count	1

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.30 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	110091

Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex

1. Protein Identity and Related Structures Protein Identity & Related Structures

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

6ezn__assembly_1__model_1

6ezn__assembly_1__model_1 | I(q)

6ezn__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (14)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer