6ezn

Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex

Method: ELECTRON MICROSCOPY

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

物种未注明

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	其他组合	异源复合物	蛋白 8 其他聚合物 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	OST1_YEAST
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–476； UniProt 1–476

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2

物种未注明

UniProt P46964

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	其他组合	异源复合物	蛋白 8 其他聚合物 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	OST2_YEAST
Isoform	—
PDB实体	2
链与序列区间	作者链 B； PDB构建体 1–130； UniProt 1–130

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3

物种未注明

UniProt P48439

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	其他组合	异源复合物	蛋白 8 其他聚合物 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	OST3_YEAST
Isoform	—
PDB实体	3
链与序列区间	作者链 C； PDB构建体 1–350； UniProt 1–350

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4

物种未注明

UniProt Q99380

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	其他组合	异源复合物	蛋白 8 其他聚合物 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	OST4_YEAST
Isoform	—
PDB实体	4
链与序列区间	作者链 D； PDB构建体 1–36； UniProt 1–36

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5

物种未注明

UniProt Q92316

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	其他组合	异源复合物	蛋白 8 其他聚合物 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	OST5_YEAST
Isoform	—
PDB实体	5
链与序列区间	作者链 E； PDB构建体 1–86； UniProt 1–86

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3

物种未注明

UniProt P39007

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	其他组合	异源复合物	蛋白 8 其他聚合物 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	STT3_YEAST
Isoform	—
PDB实体	6
链与序列区间	作者链 F； PDB构建体 1–718； UniProt 1–718

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

物种未注明

UniProt P33767

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	其他组合	异源复合物	蛋白 8 其他聚合物 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 × 1 （Q02795） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	OSTB_YEAST
Isoform	—
PDB实体	7
链与序列区间	作者链 G； PDB构建体 1–430； UniProt 1–430

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1

物种未注明

UniProt Q02795

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	其他组合	异源复合物	蛋白 8 其他聚合物 4	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 × 1 （P41543） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 × 1 （P46964） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 × 1 （P48439） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 × 1 （Q99380） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 × 1 （Q92316） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 × 1 （P39007） Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 × 1 （P33767） beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 1 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 4 PHOSPHATIDYLETHANOLAMINE × 1 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	与蛋白数一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	OSTD_YEAST
Isoform	—
PDB实体	8
链与序列区间	作者链 H； PDB构建体 1–286； UniProt 1–286

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	6ezn
沉积日期 deposition_date	2017-11-16
结构标题 title	Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex
关键词 keywords	OST complex, oligosaccharyltransferase, N-linked glycosylation, yeast, MEMBRANE PROTEIN; MEMBRANE PROTEIN
实验方法 method	ELECTRON MICROSCOPY

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3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

6ezn__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

6ezn__assembly_1__model_1 | I(q)

100 个绘图点；横轴和纵轴均采用对数尺度。

6ezn__assembly_1__model_1 | P(r) · Pending

P(r) 将按相同 assembly/model 分别计算和展示。

Rg（Guinier）	46.41 Å
Rg（电子密度）	46.21 Å
总 Rg	46.42 Å
原子数	17008
残基数	2090
排除体积	307390 Å³
最大 q	0.500 Å⁻¹

Assembly	Model	结构单元	聚集说明	状态	CRYSOL	操作
1	1	`6ezn__assembly_1__model_1`	octameric （8）	成功	4.1.3-1-20251215 (887e7ef)	查看下载

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合物信息 Entities & Polymers (14)

实体 1 Entity 1 polymer

描述 description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
分子量 formula_weight	54116.5 kDa
EC 编号 ec_number	2.4.99.18
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	17008

MRQVWFSWIVGLFLCFFNVSSAAQYEPPATWENVDYKRTIDVSNAYISETIEITIKNIASEPATEYFTAFESGIFSKVSFFSAYFTNEATFLNSQLLANSTTAPGDDGESEIRYGIIQFPNAISPQEEVSLVIKSFYNTVGIPYPEHVGMSEEQHLLWETNRLPLSAYDTKKASFTLIGSSSFEEYHPPNDESLLGKANGNSFEFGPWEDIPRFSSNETLAIVYSHNAPLNQVVNLRRDIWLSHWASTIQFEEYYELTNKAAKLSKGFSRLELMKQIQTQNMRQTHFVTVLDMLLPEGATDHYFTDLVGLVSTSHAERDHFFIRPRFPIFGGWNYNFTVGWTNKLSDFLHVSSGSDEKFVASIPILNGPPDTVYDNVELSVFLPEGAEIFDIDSPVPFTNVSIETQKSYFDLNKGHVKLTFSYRNLISQVANGQVLIKYDYPKSSFFKKPLSIACYIFTALMGVFVLKTLNMNVTN

实体 2 Entity 2 polymer

描述 description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2
分子量 formula_weight	14712.5 kDa
EC 编号 ec_number	2.4.99.18
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	B

MAKAPKANTPKVTSTSSAVLTDFQETFKTSKRAYFAQIEKYPKLKLIDTFCFFLVLLGVIQCTFIILIRDNFPFNAFLAGFIICVGQFVLLMSLRLQLCNSFPGISKNRAFAEFIVASLILHFVCLHFIN

实体 3 Entity 3 polymer

描述 description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3
分子量 formula_weight	39518.2 kDa
EC 编号 ec_number	2.4.99.18
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	C

MNWLFLVSLVFFCGVSTHPALAMSSNRLLKLANKSPKKIIPLKDSSFENILAPPHENAYIVALFTATAPEIGCSLCLELESEYDTIVASWFDDHPDAKSSNSDTSIFFTKVNLEDPSKTIPKAFQFFQLNNVPRLFIFKPNSPSILDHSVISISTDTGSERMKQIIQAIKQFSQVNDFSLHLPMDWTPIITSTIITFITVLLFKKQSKLMFSIISSRIIWATLSTFFIICMISAYMFNQIRNTQLAGVGPKGEVMYFLPNEFQHQFAIETQVMVLIYGTLAALVVVLVKGIQFLRSHLYPETKKAYFIDAILASFCALFIYVFFAALTTVFTIKSPAYPFPLLRLSAPFK

实体 4 Entity 4 polymer

描述 description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4
分子量 formula_weight	3986.7 kDa
EC 编号 ec_number	2.4.99.18
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	D

MISDEQLNSLAITFGIVMMTLIVIYHAVDSTMSPKN

实体 5 Entity 5 polymer

描述 description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5
分子量 formula_weight	9525.1 kDa
EC 编号 ec_number	2.4.99.18
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	E

MTYEQLYKEFHSSKSFQPFIHLDTQPKFAICGLIVTLAVLSSALFAVGSKSSYIKKLFFYTILSVIGSLFAGLTTVFASNSFGVYV

实体 6 Entity 6 polymer

描述 description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
分子量 formula_weight	81604.5 kDa
EC 编号 ec_number	2.4.99.18
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	F

MGSDRSCVLSVFQTILKLVIFVAIFGAAISSRLFAVIKFESIIHEFDPWFNYRATKYLVNNSFYKFLNWFDDRTWYPLGRVTGGTLYPGLMTTSAFIWHALRNWLGLPIDIRNVCVLFAPLFSGVTAWATYEFTKEIKDASAGLLAAGFIAIVPGYISRSVAGSYDNEAIAITLLMVTFMFWIKAQKTGSIMHATCAALFYFYMVSAWGGYVFITNLIPLHVFLLILMGRYSSKLYSAYTTWYAIGTVASMQIPFVGFLPIRSNDHMAALGVFGLIQIVAFGDFVKGQISTAKFKVIMMVSLFLILVLGVVGLSALTYMGLIAPWTGRFYSLWDTNYAKIHIPIIASVSEHQPVSWPAFFFDTHFLIWLFPAGVFLLFLDLKDEHVFVIAYSVLCSYFAGVMVRLMLTLTPVICVSAAVALSKIFDIYLDFKTSDRKYAIKPAALLAKLIVSGSFIFYLYLFVFHSTWVTRTAYSSPSVVLPSQTPDGKLALIDDFREAYYWLRMNSDEDSKVAAWWDYGYQIGGMADRTTLVDNNTWNNTHIAIVGKAMASPEEKSYEILKEHDVDYVLVIFGGLIGFGGDDINKFLWMIRISEGIWPEEIKERDFYTAEGEYRVDARASETMRNSLLYKMSYKDFPQLFNGGQATDRVRQQMITPLDVPPLDYFDEVFTSENWMVRIYQLKKDDAQGRTLRDVGELTRSSTKTRRSIKRPELGLRV

实体 7 Entity 7 polymer

描述 description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1
分子量 formula_weight	49444.4 kDa
EC 编号 ec_number	2.4.99.18
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	G

MRTDWNFFFCILLQAIFVVGTQTSRTLVLYDQSTEPLEEYSVYLKDLEQRNYKLEYLDINSTSTTVDLYDKEQRLFDNIIVFPTKGGKNLARQIPVKQLIKFFENEGNILCMSSPGAVPNTIRLFLNELGIYPSPKGHVIRDYFSPSSEELVVSSNHLLNKYVYNARKSEDFVFGESSAALLENREQIVPILNAPRTSFTESKGKCNSWTSGSQGFLVVGFQNLNNARLVWIGSSDFLKNKNQDSNQEFAKELLKWTFNEKSVIKSVHAVHSHADGTSYDEEPYKIKDKVIYSVGFSEWNGEEWLPHIADDIQFELRQVDPYYRLTLSPSGNDSETQYYTTGEFILPDRHGVFTFLTDYRKIGLSFTTDKDVKAIRHLANDEYPRSWEISNSWVYISAICGVIVAWIFFVVSFVTTSSVGKKLETFKKTN

实体 8 Entity 8 polymer

描述 description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1
分子量 formula_weight	31682.8 kDa
EC 编号 ec_number	2.4.99.18
来源方法 src_method	nat
拷贝数 entity_count	1

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	H

MQFFKTLAALVSCISFVLAYVAQDVHVSFPSTAGKSRVMIGKVEPRIGIDETVPTTITVEDPNEVIQVNFAIESTNKPFQNTLLIGLPNKNLEMAFEPEIKDNGKLSMYKYRIDLAKLDAALLQEASRSPEPIKATLILASSTAKPKENLFREILQLNLNFDVDHSDSSLVDKFGIKPEIHHIFHAEPKRVAKPIAVIFVLIIFITILSLIVTWLNSCAAAFNNIPTGVTAVYFLGFIATIVGFEVIFARYYLGTSIFETLFSSLYLGAPGLLTSTKFLRSFGQTI

实体 9 Entity 9 branched

描述 description	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	586.5 kDa
来源方法 src_method	man
拷贝数 entity_count	1

实体 10 Entity 10 branched

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	424.4 kDa
来源方法 src_method	man
拷贝数 entity_count	2

实体 11 Entity 11 branched

描述 description	;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
分子量 formula_weight	1397.2 kDa
来源方法 src_method	man
拷贝数 entity_count	1

实体 12 Entity 12 non-polymer

描述 description	1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE
分子量 formula_weight	758.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

实体 13 Entity 13 non-polymer

描述 description	PHOSPHATIDYLETHANOLAMINE
分子量 formula_weight	734.0 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

实体 14 Entity 14 non-polymer

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	221.2 kDa
来源方法 src_method	man
拷贝数 entity_count	1

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	1

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.30 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	110091

Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

6ezn__assembly_1__model_1

6ezn__assembly_1__model_1 | I(q)

6ezn__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合物信息 Entities & Polymers (14)

实体 1 Entity 1 polymer

聚合物 Polymer

实体 2 Entity 2 polymer

聚合物 Polymer